umuD

Summary

Gene Symbol: umuD
Description: translesion error-prone DNA polymerase V subunit; RecA-activated auto-protease
Alias: ECK1171, JW1172
Species: Escherichia coli str. K-12 substr. MG1655
Products:     umuD

Top Publications

  1. Pham P, Seitz E, Saveliev S, Shen X, Woodgate R, Cox M, et al. Two distinct modes of RecA action are required for DNA polymerase V-catalyzed translesion synthesis. Proc Natl Acad Sci U S A. 2002;99:11061-6 pubmed
    ..The RecA1730 mutant protein, which was identified on the basis of its inability to promote pol V (UmuD'(2)C)-dependent UV-mutagenesis, appears proficient for the first mode of RecA action but is deficient in the second ..
  2. Maor Shoshani A, Livneh Z. Analysis of the stimulation of DNA polymerase V of Escherichia coli by processivity proteins. Biochemistry. 2002;41:14438-46 pubmed
    ..lesions in Escherichia coli is carried out by DNA polymerase V (UmuC) in a reaction that requires UmuD', RecA, and single-strand DNA-binding protein (SSB)...
  3. Silva M, Nevin P, Ronayne E, Beuning P. Selective disruption of the DNA polymerase III ?-? complex by the umuD gene products. Nucleic Acids Res. 2012;40:5511-22 pubmed publisher
    ..The umuD gene products, which are up-regulated during the SOS response, were previously shown to bind to the ?, ? and ? ..
  4. O Grady P, Borden A, Vandewiele D, Ozgenc A, Woodgate R, Lawrence C. Intrinsic polymerase activities of UmuD'(2)C and MucA'(2)B are responsible for their different mutagenic properties during bypass of a T-T cis-syn cyclobutane dimer. J Bacteriol. 2000;182:2285-91 pubmed
    In wild-type Escherichia coli, translesion replication is largely dependent upon the UmuD'(2)C complex (DNA polymerase V [polV]) or its plasmid-encoded homologs, such as MucA'(2)B...
  5. Friedberg E, Fischhaber P, Kisker C. Error-prone DNA polymerases: novel structures and the benefits of infidelity. Cell. 2001;107:9-12 pubmed
  6. Bruck I, Woodgate R, McEntee K, Goodman M. Purification of a soluble UmuD'C complex from Escherichia coli. Cooperative binding of UmuD'C to single-stranded DNA. J Biol Chem. 1996;271:10767-74 pubmed
    The Escherichia coli UmuD' and UmuC proteins play essential roles in SOS-induced mutagenesis. Previous studies investigating the molecular mechanisms of mutagenesis have been hindered by the lack of availability of a soluble UmuC protein...
  7. Rajagopalan M, Lu C, Woodgate R, O Donnell M, Goodman M, Echols H. Activity of the purified mutagenesis proteins UmuC, UmuD', and RecA in replicative bypass of an abasic DNA lesion by DNA polymerase III. Proc Natl Acad Sci U S A. 1992;89:10777-81 pubmed
    ..is a marked increase in mutation rate, dependent on RecA protein and the induced mutagenesis proteins UmuC and UmuD. A variety of previous indirect approaches have indicated that SOS mutagenesis results from replicative bypass of ..
  8. Gonzalez M, Frank E, Levine A, Woodgate R. Lon-mediated proteolysis of the Escherichia coli UmuD mutagenesis protein: in vitro degradation and identification of residues required for proteolysis. Genes Dev. 1998;12:3889-99 pubmed
    Most SOS mutagenesis in Escherichia coli is dependent on the UmuD and UmuC proteins. Perhaps as a consequence, the activity of these proteins is exquisitely regulated...
  9. Delmas S, Matic I. Interplay between replication and recombination in Escherichia coli: impact of the alternative DNA polymerases. Proc Natl Acad Sci U S A. 2006;103:4564-9 pubmed

More Information

Publications66

  1. Simon S, Sousa F, Mohana Borges R, Walker G. Regulation of Escherichia coli SOS mutagenesis by dimeric intrinsically disordered umuD gene products. Proc Natl Acad Sci U S A. 2008;105:1152-7 pubmed publisher
    Products of the umuD gene in Escherichia coli play key roles in coordinating the switch from accurate DNA repair to mutagenic translesion DNA synthesis (TLS) during the SOS response to DNA damage...
  2. Marsh L, Walker G. Cold sensitivity induced by overproduction of UmuDC in Escherichia coli. J Bacteriol. 1985;162:155-61 pubmed
    The UmuD and UmuC proteins of Escherichia coli are essential for mutagenesis by UV and most chemicals. Their mode of action is presently unknown...
  3. Shinagawa H, Iwasaki H, Kato T, Nakata A. RecA protein-dependent cleavage of UmuD protein and SOS mutagenesis. Proc Natl Acad Sci U S A. 1988;85:1806-10 pubmed
    ..Products of the recA and umuD,C genes are required for mutagenesis induced by radiation and many chemicals...
  4. Borden A, O Grady P, Vandewiele D, Fernández de Henestrosa A, Lawrence C, Woodgate R. Escherichia coli DNA polymerase III can replicate efficiently past a T-T cis-syn cyclobutane dimer if DNA polymerase V and the 3' to 5' exonuclease proofreading function encoded by dnaQ are inactivated. J Bacteriol. 2002;184:2674-81 pubmed
    ..This mutant polymerase does not replicate past the much more distorted T-T (6-4) photoadduct, however, suggesting that it may only replicate past lesions, like the T-T dimer, that form base pairs normally. ..
  5. Fujii S, Gasser V, Fuchs R. The biochemical requirements of DNA polymerase V-mediated translesion synthesis revisited. J Mol Biol. 2004;341:405-17 pubmed
    ..Genetic studies have established that mutagenesis requires both UmuC and a proteolytic product of UmuD (UmuD')...
  6. Tang M, Pham P, Shen X, Taylor J, O Donnell M, Woodgate R, et al. Roles of E. coli DNA polymerases IV and V in lesion-targeted and untargeted SOS mutagenesis. Nature. 2000;404:1014-8 pubmed
    The expression of the Escherichia coli DNA polymerases pol V (UmuD'2C complex) and pol IV (DinB) increases in response to DNA damage...
  7. Tang M, Shen X, Frank E, O Donnell M, Woodgate R, Goodman M. UmuD'(2)C is an error-prone DNA polymerase, Escherichia coli pol V. Proc Natl Acad Sci U S A. 1999;96:8919-24 pubmed
    The damage-inducible UmuD' and UmuC proteins are required for most SOS mutagenesis in Escherichia coli...
  8. Sommer S, Bailone A, Devoret R. The appearance of the UmuD'C protein complex in Escherichia coli switches repair from homologous recombination to SOS mutagenesis. Mol Microbiol. 1993;10:963-71 pubmed
    ..in Escherichia coli requires (i) the replisome enzymes, (ii) RecA protein, and (iii) the formation of the UmuD'C protein complex which appears to help the replisome to resume DNA synthesis across a lesion...
  9. Tang M, Bruck I, Eritja R, Turner J, Frank E, Woodgate R, et al. Biochemical basis of SOS-induced mutagenesis in Escherichia coli: reconstitution of in vitro lesion bypass dependent on the UmuD'2C mutagenic complex and RecA protein. Proc Natl Acad Sci U S A. 1998;95:9755-60 pubmed
    Damage-induced SOS mutagenesis requiring the UmuD'C proteins occurs as part of the cells' global response to DNA damage...
  10. McDonald J, Maury E, Levine A, Woodgate R. Regulation of UmuD cleavage: role of the amino-terminal tail. J Mol Biol. 1998;282:721-30 pubmed
    An essential step in SOS mutagenesis is the RecA-mediated posttranslational processing of UmuD-like proteins to the shorter, but mutagenically active, UmuD'-like proteins...
  11. Schlacher K, Leslie K, Wyman C, Woodgate R, Cox M, Goodman M. DNA polymerase V and RecA protein, a minimal mutasome. Mol Cell. 2005;17:561-72 pubmed
    ..TLS requires DNA polymerase V (UmuD'2C) and RecA. Here, we show that pol V and RecA interact by two distinct mechanisms...
  12. Becherel O, Fuchs R, Wagner J. Pivotal role of the beta-clamp in translesion DNA synthesis and mutagenesis in E. coli cells. DNA Repair (Amst). 2002;1:703-8 pubmed
    ..Thus, in addition to its crucial role as the processivity factor of the PolIII replicase, the beta-clamp plays a pivotal role during the TLS process. ..
  13. Gonzalez M, Rasulova F, Maurizi M, Woodgate R. Subunit-specific degradation of the UmuD/D' heterodimer by the ClpXP protease: the role of trans recognition in UmuD' stability. EMBO J. 2000;19:5251-8 pubmed
    The Escherichia coli UmuD' protein is a subunit of the recently described error-prone DNA polymerase, pol V. UmuD' is initially synthesized as an unstable and mutagenically inactive pro-protein, UmuD...
  14. Tago Y, Imai M, Ihara M, Atofuji H, Nagata Y, Yamamoto K. Escherichia coli mutator (Delta)polA is defective in base mismatch correction: the nature of in vivo DNA replication errors. J Mol Biol. 2005;351:299-308 pubmed
    ..The mutation rate of this strain was 219x10(-8), about a 100-fold increase relative to the wild-type strain. Uncorrected polymerase III replication errors were predominantly frameshifts and base substitutions followed by deletions. ..
  15. Sutton M, Opperman T, Walker G. The Escherichia coli SOS mutagenesis proteins UmuD and UmuD' interact physically with the replicative DNA polymerase. Proc Natl Acad Sci U S A. 1999;96:12373-8 pubmed
    ..b>UmuD protein then undergoes an RecA-facilitated self-cleavage reaction that removes its N-terminal 24 residues to yield ..
  16. Rangarajan S, Woodgate R, Goodman M. A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli. Proc Natl Acad Sci U S A. 1999;96:9224-9 pubmed
    ..strains containing pol II, the restart process is delayed for >90 min in cells lacking both pol II and UmuD'(2)C...
  17. Neher S, Sauer R, Baker T. Distinct peptide signals in the UmuD and UmuD' subunits of UmuD/D' mediate tethering and substrate processing by the ClpXP protease. Proc Natl Acad Sci U S A. 2003;100:13219-24 pubmed
    The Escherichia coli UmuD' protein is a component of DNA polymerase V, an error-prone polymerase that carries out translesion synthesis on damaged DNA templates...
  18. Beuning P, Sawicka D, Barsky D, Walker G. Two processivity clamp interactions differentially alter the dual activities of UmuC. Mol Microbiol. 2006;59:460-74 pubmed
    ..cause loss of the cold-sensitive and beta-dependent synthetic lethal phenotypes associated with increased levels of UmuD and UmuC that are thought to represent an exaggeration of a DNA damage checkpoint...
  19. Maor Shoshani A, Reuven N, Tomer G, Livneh Z. Highly mutagenic replication by DNA polymerase V (UmuC) provides a mechanistic basis for SOS untargeted mutagenesis. Proc Natl Acad Sci U S A. 2000;97:565-70 pubmed
    ..This reaction required, in addition to pol V, UmuD', RecA, and single-stranded DNA (ssDNA)-binding protein. pol V produced point mutations at a frequency of 2...
  20. Al Mamun A, Humayun M. Spontaneous mutagenesis is elevated in protease-defective cells. Mol Microbiol. 2009;71:629-39 pubmed publisher
    ..In contrast, mutagenesis is unaffected or stimulated in cells deleted for umuC or umuD, which code for critical components of DNA polymerase V...
  21. Fernandez de Henestrosa A, Ogi T, Aoyagi S, Chafin D, Hayes J, Ohmori H, et al. Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol Microbiol. 2000;35:1560-72 pubmed
    ..These experiments have allowed us to identify seven new LexA-regulated genes, thus bringing the present number of genes in the E. coli LexA regulon to 31. The potential function of each newly identified LexA-regulated gene is discussed. ..
  22. Frank E, Ennis D, Gonzalez M, Levine A, Woodgate R. Regulation of SOS mutagenesis by proteolysis. Proc Natl Acad Sci U S A. 1996;93:10291-6 pubmed
    DNA damage-inducible mutagenesis in Escherichia coli is largely dependent upon the activity of the UmuD (UmuD') and UmuC proteins...
  23. Burckhardt S, Woodgate R, Scheuermann R, Echols H. UmuD mutagenesis protein of Escherichia coli: overproduction, purification, and cleavage by RecA. Proc Natl Acad Sci U S A. 1988;85:1811-5 pubmed
    ..This enhanced mutation rate requires the action of the UmuD and UmuC proteins, which are induced as part of the SOS response to DNA damage...
  24. Sutton M, Walker G. umuDC-mediated cold sensitivity is a manifestation of functions of the UmuD(2)C complex involved in a DNA damage checkpoint control. J Bacteriol. 2001;183:1215-24 pubmed
    ..After induction, they help to promote cell survival via two temporally separate pathways. First, UmuD and UmuC together participate in a cell cycle checkpoint control; second, UmuD'(2)C enables translesion DNA ..
  25. Ollivierre J, Budil D, Beuning P. Electron spin labeling reveals the highly dynamic N-terminal arms of the SOS mutagenesis protein UmuD. Mol Biosyst. 2011;7:3183-6 pubmed publisher
    ..resonance (EPR) spectroscopy was used to probe the conformational dynamics of the N-terminal arms of the umuD gene products...
  26. Rehrauer W, Bruck I, Woodgate R, Goodman M, Kowalczykowski S. Modulation of RecA nucleoprotein function by the mutagenic UmuD'C protein complex. J Biol Chem. 1998;273:32384-7 pubmed
    The RecA, UmuC, and UmuD' proteins are essential for error-prone, replicative bypass of DNA lesions. Normally, RecA protein mediates homologous pairing of DNA. We show that purified Umu(D')2C blocks this recombination function...
  27. Beuning P, Simon S, Godoy V, Jarosz D, Walker G. Characterization of Escherichia coli translesion synthesis polymerases and their accessory factors. Methods Enzymol. 2006;408:318-40 pubmed
    ..Escherichia coli possesses two members of the Y family, DNA pol IV (DinB) and DNA pol V (UmuD'(2)C), and several regulatory factors, including those encoded by the umuD gene that influence the activity of UmuC...
  28. Sutton M, Smith B, Godoy V, Walker G. The SOS response: recent insights into umuDC-dependent mutagenesis and DNA damage tolerance. Annu Rev Genet. 2000;34:479-497 pubmed
    ..Recent advances have focused attention on the umuD(+)C(+)-dependent, translesion DNA synthesis (TLS) process that is responsible for SOS mutagenesis ()...
  29. Napolitano R, Janel Bintz R, Wagner J, Fuchs R. All three SOS-inducible DNA polymerases (Pol II, Pol IV and Pol V) are involved in induced mutagenesis. EMBO J. 2000;19:6259-65 pubmed
    ..Therefore, in response to the vast diversity of existing DNA damage, the cell uses a pool of 'translesional' DNA polymerases in order to bypass the various DNA lesions. ..
  30. Beuning P, Simon S, Zemla A, Barsky D, Walker G. A non-cleavable UmuD variant that acts as a UmuD' mimic. J Biol Chem. 2006;281:9633-40 pubmed
    b>UmuD(2) cleaves and removes its N-terminal 24 amino acids to form UmuD'(2), which activates UmuC for its role in UV-induced mutagenesis in Escherichia coli...
  31. Yasuda T, Morimatsu K, Kato R, Usukura J, Takahashi M, Ohmori H. Physical interactions between DinI and RecA nucleoprotein filament for the regulation of SOS mutagenesis. EMBO J. 2001;20:1192-202 pubmed
    ..Its overexpression conferred severe UV sensitivity on wild-type cells and resulted in the inhibition of LexA and UmuD processing, reactions that are normally dependent on activated RecA in a complex with single-stranded (ss)DNA...
  32. Sutton M, Guzzo A, Narumi I, Costanzo M, Altenbach C, Ferentz A, et al. A model for the structure of the Escherichia coli SOS-regulated UmuD2 protein. DNA Repair (Amst). 2002;1:77-93 pubmed
    ..it is becoming evident that a sophisticated series of protein-protein interactions involving the two forms of the umuD gene product, UmuD and UmuD' and components of the replicative DNA polymerase serve to manage the actions of the ..
  33. López de Saro F, Georgescu R, Goodman M, O Donnell M. Competitive processivity-clamp usage by DNA polymerases during DNA replication and repair. EMBO J. 2003;22:6408-18 pubmed
    ..Given the limited amounts of clamps in the cell, these results suggest that clamp binding may be competitive and regulated, and that the different polymerases may use the same clamp sequentially during replication and repair. ..
  34. Friedman N, Vardi S, Ronen M, Alon U, Stavans J. Precise temporal modulation in the response of the SOS DNA repair network in individual bacteria. PLoS Biol. 2005;3:e238 pubmed
    ..umuDC operon in maintaining the pattern of PA and its temporal precision, providing further evidence for the role UmuD cleavage plays in effecting a timed pause during the SOS response, as previously proposed...
  35. Schlacher K, Cox M, Woodgate R, Goodman M. RecA acts in trans to allow replication of damaged DNA by DNA polymerase V. Nature. 2006;442:883-7 pubmed
  36. Goodman M. Error-prone repair DNA polymerases in prokaryotes and eukaryotes. Annu Rev Biochem. 2002;71:17-50 pubmed
    ..Macromolecular assemblies of replication-repair "factories" could enable a cell to handle the complex logistics governing the rapid migration and exchange of polymerases. ..
  37. Sutton M, Narumi I, Walker G. Posttranslational modification of the umuD-encoded subunit of Escherichia coli DNA polymerase V regulates its interactions with the beta processivity clamp. Proc Natl Acad Sci U S A. 2002;99:5307-12 pubmed
    ..Interactions of the two umuD gene products, the 139-aa UmuD and the 115-aa UmuD' proteins, with components of the replicative DNA polymerase (..
  38. Yeiser B, Pepper E, Goodman M, Finkel S. SOS-induced DNA polymerases enhance long-term survival and evolutionary fitness. Proc Natl Acad Sci U S A. 2002;99:8737-41 pubmed
    ..These polymerases contribute to survival by providing essential functions to ensure replication of the chromosome and by generating genetic diversity. ..
  39. Woodgate R, Ennis D. Levels of chromosomally encoded Umu proteins and requirements for in vivo UmuD cleavage. Mol Gen Genet. 1991;229:10-6 pubmed
    ..observed in Escherichia coli after treatment with many DNA damaging agents is dependent upon the products of the umuD,C operon...
  40. Woodgate R, Rajagopalan M, Lu C, Echols H. UmuC mutagenesis protein of Escherichia coli: purification and interaction with UmuD and UmuD'. Proc Natl Acad Sci U S A. 1989;86:7301-5 pubmed
    ..process dependent on two RecA-mediated proteolytic events: cleavage of the LexA repressor to induce the UmuC and UmuD mutagenesis proteins, and cleavage of UmuD to UmuD' to activate the mutation pathway...
  41. Sutton M, Farrow M, Burton B, Walker G. Genetic interactions between the Escherichia coli umuDC gene products and the beta processivity clamp of the replicative DNA polymerase. J Bacteriol. 2001;183:2897-909 pubmed
    ..distinct roles of the umuDC gene products are regulated by RecA-single-stranded DNA-facilitated self-cleavage of UmuD (which participates in the checkpoint control) to yield UmuD' (which enables TLS)...
  42. Opperman T, Murli S, Walker G. The genetic requirements for UmuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis. J Bacteriol. 1996;178:4400-11 pubmed
    ..Following the posttranslational processing of UmuD to UmuD' by RecA-mediated cleavage, UmuD' acts in concert with UmuC, RecA, and DNA polymerase III to facilitate the ..
  43. Frank E, Hauser J, Levine A, Woodgate R. Targeting of the UmuD, UmuD', and MucA' mutagenesis proteins to DNA by RecA protein. Proc Natl Acad Sci U S A. 1993;90:8169-73 pubmed
    ..depression of the SOS regulon by mediating the posttranslational cleavage of the LexA repressor, (ii) activation of UmuD'-like proteins by mediating cleavage of the UmuD-like proteins, and (iii) a direct step, possibly to interact with ..
  44. Erdem A, Jaszczur M, Bertram J, Woodgate R, Cox M, Goodman M. DNA polymerase V activity is autoregulated by a novel intrinsic DNA-dependent ATPase. elife. 2014;3:e02384 pubmed publisher
    Escherichia coli DNA polymerase V (pol V), a heterotrimeric complex composed of UmuD'2C, is marginally active. ATP and RecA play essential roles in the activation of pol V for DNA synthesis including translesion synthesis (TLS)...
  45. Nohmi T, Battista J, Dodson L, Walker G. RecA-mediated cleavage activates UmuD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation. Proc Natl Acad Sci U S A. 1988;85:1816-20 pubmed
    ..It has been shown that the UmuD protein shares homology with LexA, the repressor of the SOS genes...
  46. Godoy V, Jarosz D, Simon S, Abyzov A, Ilyin V, Walker G. UmuD and RecA directly modulate the mutagenic potential of the Y family DNA polymerase DinB. Mol Cell. 2007;28:1058-70 pubmed
    ..We show that the DNA damage-inducible proteins UmuD(2) and RecA act in concert to modulate this mutagenic activity...
  47. Peat T, Frank E, McDonald J, Levine A, Woodgate R, Hendrickson W. Structure of the UmuD' protein and its regulation in response to DNA damage. Nature. 1996;380:727-30 pubmed publisher
    ..Essential proteins in this mutagenic process are RecA, DNA polymerase III, UmuD, UmuD' and UmuC (umu: UV mutagenesis)...
  48. Boudsocq F, Campbell M, Devoret R, Bailone A. Quantitation of the inhibition of Hfr x F- recombination by the mutagenesis complex UmuD'C. J Mol Biol. 1997;270:201-11 pubmed
    The UmuD'C complex and RecA protein are two essential components in mutagenic repair of gaps produced by the replication of damaged DNA. In this process, the UmuD'C complex might help DNA polymerase to synthesize DNA across a lesion...
  49. Opperman T, Murli S, Smith B, Walker G. A model for a umuDC-dependent prokaryotic DNA damage checkpoint. Proc Natl Acad Sci U S A. 1999;96:9218-23 pubmed
    ..The UmuD protein shares homology with LexA, the repressor of SOS-regulated loci, and similarly undergoes a facilitated ..
  50. Woodgate R. Construction of a umuDC operon substitution mutation in Escherichia coli. Mutat Res. 1992;281:221-5 pubmed
    ..It is concluded that the UmuDC proteins, which are normally required for inducible mutagenesis, are not essential for cell survival. ..
  51. Sutton M, Walker G. Managing DNA polymerases: coordinating DNA replication, DNA repair, and DNA recombination. Proc Natl Acad Sci U S A. 2001;98:8342-9 pubmed
    ..In particular, we discuss these issues in the context of the Gram-negative bacterium, Escherichia coli, that contains a DNA polymerase (Pol V) known to participate in most, if not all, of these processes. ..
  52. Sommer S, Becherel O, Coste G, Bailone A, Fuchs R. Altered translesion synthesis in E. coli Pol V mutants selected for increased recombination inhibition. DNA Repair (Amst). 2003;2:1361-9 pubmed
    ..depending on the nature of the lesion, it generally requires the Pol V DNA polymerase (formed by two SOS proteins, UmuD' and UmuC) and the RecA protein...
  53. Rangarajan S, Woodgate R, Goodman M. Replication restart in UV-irradiated Escherichia coli involving pols II, III, V, PriA, RecA and RecFOR proteins. Mol Microbiol. 2002;43:617-28 pubmed
    ..In con-trast, only RecF is required for the activation of RecA that leads to the formation of pol V (UmuD'2C) and facilitates replication readthrough.
  54. Reuven N, Arad G, Maor Shoshani A, Livneh Z. The mutagenesis protein UmuC is a DNA polymerase activated by UmuD', RecA, and SSB and is specialized for translesion replication. J Biol Chem. 1999;274:31763-6 pubmed
    ..coli this process is regulated by the SOS stress response, and requires the mutagenesis proteins UmuC and UmuD'. Analysis of translesion replication using a recently reconstituted in vitro system (Reuven, N. B., Tomer, G...
  55. Sommer S, Coste G, Bailone A. Specific amino acid changes enhance the anti-recombination activity of the UmuD'C complex. Mol Microbiol. 2000;35:1443-53 pubmed
    In addition to being an essential component of trans-lesion synthesis, the UmuD'C complex is an antagonist of RecA-mediated homologous recombination...
  56. Jiang Q, Karata K, Woodgate R, Cox M, Goodman M. The active form of DNA polymerase V is UmuD'(2)C-RecA-ATP. Nature. 2009;460:359-63 pubmed publisher
    ..We show that RecA* transfers a single RecA-ATP stoichiometrically from its DNA 3'-end to free pol V (UmuD'(2)C) to form an active mutasome (pol V Mut) with the composition UmuD'(2)C-RecA-ATP...
  57. Duzen J, Walker G, Sutton M. Identification of specific amino acid residues in the E. coli beta processivity clamp involved in interactions with DNA polymerase III, UmuD and UmuD'. DNA Repair (Amst). 2004;3:301-12 pubmed
    ..match to this motif, which appears to be required for their respective interactions with the clamp, neither UmuD not UmuD' do. As part of an ongoing effort to understand how interactions involving the different E...