Gene Symbol: uhpB
Description: sensory histidine kinase in two-component regulatory sytem with UhpA
Alias: ECK3659, JW3643
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Island M, Kadner R. Interplay between the membrane-associated UhpB and UhpC regulatory proteins. J Bacteriol. 1993;175:5028-34 pubmed
    ..The UhpA and UhpB proteins are related to the response-regulator and sensor-kinase proteins of two-component regulatory systems, ..
  2. Verhamme D, Postma P, Crielaard W, Hellingwerf K. Cooperativity in signal transfer through the Uhp system of Escherichia coli. J Bacteriol. 2002;184:4205-10 pubmed
    ..of extracellular glucose 6-phosphate by membrane-bound UhpC, which in turn modulates the histidine-protein kinase UhpB. Together with the cytoplasmic response regulator UhpA, they constitute a typical two-component regulatory system ..
  3. Island M, Wei B, Kadner R. Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1992;174:2754-62 pubmed
    ..Whereas the deletion of uhpA completely abolished the expression of a uhpT-lacZ reporter gene, the deletion of uhpB or uhpC resulted in a partially elevated basal level of expression that was not further inducible...
  4. Friedrich M, Kadner R. Nucleotide sequence of the uhp region of Escherichia coli. J Bacteriol. 1987;169:3556-63 pubmed
    ..The 518-amino-acid UhpB and the 199-amino-acid UhpC regulatory proteins contain substantial segments of hydrophobic character...
  5. Verhamme D, Arents J, Postma P, Crielaard W, Hellingwerf K. Glucose-6-phosphate-dependent phosphoryl flow through the Uhp two-component regulatory system. Microbiology. 2001;147:3345-52 pubmed
    ..of extracellular glucose 6-phosphate (G6P), by membrane-bound UhpC, modulates a second membrane-bound protein, UhpB, resulting in autophosphorylation of a conserved histidine residue in the cytoplasmic (transmitter) domain of the ..
  6. Park J, Lee K, Kim T, Lee S. Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation. Proc Natl Acad Sci U S A. 2007;104:7797-802 pubmed
    ..These results suggest that an industrially competitive strain can be efficiently developed by metabolic engineering based on combined rational modification, transcriptome profiling, and systems-level in silico analysis. ..
  7. Merkel T, Nelson D, Brauer C, Kadner R. Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene. J Bacteriol. 1992;174:2763-70 pubmed
    ..The two halves of the -64 inverted repeat did not contribute equally to promoter function and did not have to be intact for UhpA titration. The sequence recognized by UhpA is predicted to be 5' -GGCAAAACNNNGAAA. ..
  8. Weston L, Kadner R. Identification of uhp polypeptides and evidence for their role in exogenous induction of the sugar phosphate transport system of Escherichia coli K-12. J Bacteriol. 1987;169:3546-55 pubmed
    ..The products of uhpB and uhpC are not required when uhpA is present on a multicopy plasmid...
  9. Weston L, Kadner R. Role of uhp genes in expression of the Escherichia coli sugar-phosphate transport system. J Bacteriol. 1988;170:3375-83 pubmed
    ..Expression of uhpT-lacZ fusions depended on the function of uhpA, uhpB, and uhpC but not of uhpT...

More Information


  1. Webber C, Kadner R. Action of receiver and activator modules of UhpA in transcriptional control of the Escherichia coli sugar phosphate transport system. Mol Microbiol. 1995;15:883-93 pubmed
    ..generated by in vitro manipulations and missense mutations selected for independence from the requirement for UhpB kinase activity...
  2. Kadner R, Webber C, Island M. The family of organo-phosphate transport proteins includes a transmembrane regulatory protein. J Bioenerg Biomembr. 1993;25:637-45 pubmed
    ..Of emphasis here is the unusual involvement in transmembrane signaling of the UhpC protein which is related in sequence and structure to some transport proteins, including the very protein whose synthesis it helps regulate. ..
  3. Wright J, Olekhnovich I, Touchie G, Kadner R. The histidine kinase domain of UhpB inhibits UhpA action at the Escherichia coli uhpT promoter. J Bacteriol. 2000;182:6279-86 pubmed
    ..sugar phosphate transporter in Escherichia coli in response to external glucose 6-phosphate includes the HK protein UhpB and the polytopic membrane protein UhpC, a UhpT homolog which is required for responsiveness to an inducer and ..
  4. Wright J, Kadner R. The phosphoryl transfer domain of UhpB interacts with the response regulator UhpA. J Bacteriol. 2001;183:3149-59 pubmed
    ..b>UhpB is the HK protein which controls production of the sugar phosphate transporter UhpT...
  5. Anantharaman V, Aravind L. Application of comparative genomics in the identification and analysis of novel families of membrane-associated receptors in bacteria. BMC Genomics. 2003;4:34 pubmed
    ..is predicted to be a potential sensor of murein derivatives, whereas the other one typified by the Escherichia coli UhpB protein is predicted to function as sensor of conformational changes occurring in associated membrane proteins We ..