Gene Symbol: uhpA
Description: response regulator in two-component regulatory system wtih UhpB
Alias: ECK3660, JW3644
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Parkinson J, Kofoid E. Communication modules in bacterial signaling proteins. Annu Rev Genet. 1992;26:71-112 pubmed
  2. Merkel T, Nelson D, Brauer C, Kadner R. Promoter elements required for positive control of transcription of the Escherichia coli uhpT gene. J Bacteriol. 1992;174:2763-70 pubmed
    ..The expression of uhpT, the gene encoding the transport protein, is regulated by the uhpABC gene products. The UhpA protein is required for expression; its deduced amino acid sequence shows that it belongs to a subfamily of ..
  3. Olekhnovich I, Kadner R. Mutational scanning and affinity cleavage analysis of UhpA-binding sites in the Escherichia coli uhpT promoter. J Bacteriol. 2002;184:2682-91 pubmed
    b>UhpA, a member of the NarL family of response regulators, activates transcription of the Escherichia coli uhpT gene for the sugar phosphate transporter UhpT in response to extracellular glucose-6-phosphate...
  4. Weston L, Kadner R. Identification of uhp polypeptides and evidence for their role in exogenous induction of the sugar phosphate transport system of Escherichia coli K-12. J Bacteriol. 1987;169:3546-55 pubmed
    ..The products of uhpB and uhpC are not required when uhpA is present on a multicopy plasmid...
  5. Verhamme D, Arents J, Postma P, Crielaard W, Hellingwerf K. Investigation of in vivo cross-talk between key two-component systems of Escherichia coli. Microbiology. 2002;148:69-78 pubmed
    ..UhpT expression was demonstrated when cells were grown on pyruvate, through non-cognate phosphorylation of UhpA by acetyl phosphate.
  6. Olekhnovich I, Dahl J, Kadner R. Separate contributions of UhpA and CAP to activation of transcription of the uhpT promoter of Escherichia coli. J Mol Biol. 1999;292:973-86 pubmed
    ..of the Escherichia coli uhpT gene, encoding the organophosphate transporter, requires the response regulator UhpA and is stimulated by the global regulator protein CAP...
  7. Webber C, Kadner R. Involvement of the amino-terminal phosphorylation module of UhpA in activation of uhpT transcription in Escherichia coli. Mol Microbiol. 1997;24:1039-48 pubmed
    The UhpA protein is required for expression of the sugar phosphate transporter UhpT in Escherichia coli and is regulated by phosphate transfer from the transmembrane UhpBC sensor kinase complex...
  8. Dahl J, Wei B, Kadner R. Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter. J Biol Chem. 1997;272:1910-9 pubmed
    ..These proteins are thought to regulate the phosphorylation of the transcription activator, UhpA. To examine the effect of protein phosphorylation on the binding of UhpA to target sequences in the uhpT promoter ..
  9. Park J, Lee K, Kim T, Lee S. Metabolic engineering of Escherichia coli for the production of L-valine based on transcriptome analysis and in silico gene knockout simulation. Proc Natl Acad Sci U S A. 2007;104:7797-802 pubmed
    ..These results suggest that an industrially competitive strain can be efficiently developed by metabolic engineering based on combined rational modification, transcriptome profiling, and systems-level in silico analysis. ..

More Information


  1. Verhamme D, Arents J, Postma P, Crielaard W, Hellingwerf K. Glucose-6-phosphate-dependent phosphoryl flow through the Uhp two-component regulatory system. Microbiology. 2001;147:3345-52 pubmed
    ..Subsequently, this phosphoryl group is transferred to a conserved aspartate residue in the response-regulator UhpA, which then initiates uhpT transcription, via binding to the uhpT promoter region...
  2. Olekhnovich I, Kadner R. RNA polymerase alpha and sigma(70) subunits participate in transcription of the Escherichia coli uhpT promoter. J Bacteriol. 1999;181:7266-73 pubmed
    ..coli uhpT gene, encoding the inducible sugar phosphate transporter, is dependent on the response regulator UhpA and is stimulated by the cyclic AMP receptor protein (CAP)...
  3. Kadner R, Webber C, Island M. The family of organo-phosphate transport proteins includes a transmembrane regulatory protein. J Bioenerg Biomembr. 1993;25:637-45 pubmed
    ..Of emphasis here is the unusual involvement in transmembrane signaling of the UhpC protein which is related in sequence and structure to some transport proteins, including the very protein whose synthesis it helps regulate. ..
  4. Parkinson J. Signal transduction schemes of bacteria. Cell. 1993;73:857-71 pubmed
  5. Shattuck Eidens D, Kadner R. Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli. J Bacteriol. 1983;155:1062-70 pubmed
    ..Plasmid pLC40-33 conferred inducible Uhp expression, which required the presence of the uhpA(+) gene on the chromosome...
  6. Friedrich M, Kadner R. Nucleotide sequence of the uhp region of Escherichia coli. J Bacteriol. 1987;169:3556-63 pubmed
    ..The 196-amino-acid UhpA polypeptide has the composition characteristic of a soluble protein and bears homology to the DNA-binding regions ..
  7. Island M, Wei B, Kadner R. Structure and function of the uhp genes for the sugar phosphate transport system in Escherichia coli and Salmonella typhimurium. J Bacteriol. 1992;174:2754-62 pubmed
    ..Whereas the deletion of uhpA completely abolished the expression of a uhpT-lacZ reporter gene, the deletion of uhpB or uhpC resulted in a ..
  8. Pao G, Saier M. Response regulators of bacterial signal transduction systems: selective domain shuffling during evolution. J Mol Evol. 1995;40:136-54 pubmed
    ..Sequence data were correlated with structure/function when such information was available.(ABSTRACT TRUNCATED AT 250 WORDS) ..
  9. Webber C, Kadner R. Action of receiver and activator modules of UhpA in transcriptional control of the Escherichia coli sugar phosphate transport system. Mol Microbiol. 1995;15:883-93 pubmed
    ..b>UhpA protein is required for uhpT transcription and is related to response regulators of two-component regulatory ..
  10. Wright J, Olekhnovich I, Touchie G, Kadner R. The histidine kinase domain of UhpB inhibits UhpA action at the Escherichia coli uhpT promoter. J Bacteriol. 2000;182:6279-86 pubmed
    ..could not result solely from the phosphatase activity of UhpB, because interference affected both overexpressed UhpA and UhpA variants which are active in the absence of phosphorylation...
  11. Wright J, Kadner R. The phosphoryl transfer domain of UhpB interacts with the response regulator UhpA. J Bacteriol. 2001;183:3149-59 pubmed
    ..This dominant-negative interference could result from the ability of GST-Bc to bind and sequester the RR UhpA and to accelerate its dephosphorylation...
  12. Weston L, Kadner R. Role of uhp genes in expression of the Escherichia coli sugar-phosphate transport system. J Bacteriol. 1988;170:3375-83 pubmed
    ..Expression of uhpT-lacZ fusions depended on the function of uhpA, uhpB, and uhpC but not of uhpT...
  13. Stock J, Stock A, Mottonen J. Signal transduction in bacteria. Nature. 1990;344:395-400 pubmed publisher
    ..This phosphotransfer network couples environmental signals to an array of response elements that control cell motility and regulate gene expression...
  14. Verhamme D, Postma P, Crielaard W, Hellingwerf K. Cooperativity in signal transfer through the Uhp system of Escherichia coli. J Bacteriol. 2002;184:4205-10 pubmed
    ..Together with the cytoplasmic response regulator UhpA, they constitute a typical two-component regulatory system based on His-to-Asp phosphoryl transfer. Activated (i.e...
  15. Nilsson A, Berg O, Aspevall O, Kahlmeter G, Andersson D. Biological costs and mechanisms of fosfomycin resistance in Escherichia coli. Antimicrob Agents Chemother. 2003;47:2850-8 pubmed
    ..Resistance in the mutants isolated in vitro was caused by ptsI, cyaA, glpT, uhpA/T, and unknown mutations, whereas no cyaA or ptsI mutants could be found in vivo...
  16. Kadner R, Shattuck Eidens D. Genetic control of the hexose phosphate transport system of Escherichia coli: mapping of deletion and insertion mutations in the uhp region. J Bacteriol. 1983;155:1052-61 pubmed
    ..Mapping experiments were consistent with the gene order pyrE-gltS-uhpTRA-ilvB, where uhpT encodes the transport system and uhpR and uhpA are regulatory genes whose products are necessary for proper uhp regulation.
  17. Chen Q, Kadner R. Effect of altered spacing between uhpT promoter elements on transcription activation. J Bacteriol. 2000;182:4430-6 pubmed
    ..sugar phosphate transport system in Escherichia coli, possesses multiple sites for its specific activator protein, UhpA, and a single site for binding of the global regulator, the catabolite gene activator protein (CAP)...
  18. Stock A, Robinson V, Goudreau P. Two-component signal transduction. Annu Rev Biochem. 2000;69:183-215 pubmed
    ..Thus detailed analyses of a relatively small number of representative proteins provide a foundation for understanding this large family of signaling proteins. ..