Gene Symbol: ugpA
Description: sn-glycerol-3-phosphate ABC transporter permease
Alias: ECK3436, JW3417, psiB, psiC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Mildener B, Fondy T, Engel R, Tropp B. Effects of halo analogs of glycerol 3-phosphate and dihydroxyacetone phosphate upon Escherichia coli. Antimicrob Agents Chemother. 1981;19:678-81 pubmed
    ..The fluoro and chloro analogs of glycerol 3-phosphate inhibit the growth of Escherichia coli and affect bacterial enzymes involved in lipid synthesis. ..
  2. Argast M, Ludtke D, Silhavy T, Boos W. A second transport system for sn-glycerol-3-phosphate in Escherichia coli. J Bacteriol. 1978;136:1070-83 pubmed
    ..The mutation ugp+, which was responsible for the appearance of the new transport system and the appearance of GP 1, 2, and 3 in the periplasm was cotransducible with araD by phage P1 transduction and was recessive in merodiploids. ..
  3. Kasahara M, Makino K, Amemura M, Nakata A, Shinagawa H. Dual regulation of the ugp operon by phosphate and carbon starvation at two interspaced promoters. J Bacteriol. 1991;173:549-58 pubmed
    ..These results suggest that the two promoters function alternately in responding to phosphate or carbon starvation, thus providing the cell with a means to adapt to these physiological stresses. ..
  4. Boos W. Binding protein-dependent ABC transport system for glycerol 3-phosphate of Escherichia coli. Methods Enzymol. 1998;292:40-51 pubmed
  5. Schweizer H, Boos W. Cloning of the ugp region containing the structural genes for the pho regulon-dependent sn-glycerol-3-phosphate transport system of Escherichia coli. Mol Gen Genet. 1983;192:177-86 pubmed
    ..Using ugp-lacZ fusions, it could be shown that the ugp region consists of at least two different operons that are transcribed in the same direction (counterclockwise) on the E. coli chromosome. ..
  6. Su T, Schweizer H, Oxender D. Carbon-starvation induction of the ugp operon, encoding the binding protein-dependent sn-glycerol-3-phosphate transport system in Escherichia coli. Mol Gen Genet. 1991;230:28-32 pubmed
    ..This conclusion is supported by the fact that pho-independent ugp expression correlated with the presence of the cya and crp gene products. ..
  7. Yang K, Wang M, Metcalf W. Uptake of glycerol-2-phosphate via the ugp-encoded transporter in Escherichia coli K-12. J Bacteriol. 2009;191:4667-70 pubmed publisher
    ..Mutations in the ugpBAECQ operon eliminated this phenotype, suggesting that G2P is a previously unrecognized substrate for the binding protein-dependent Ugp transporter...
  8. Brzoska P, Rimmele M, Brzostek K, Boos W. The pho regulon-dependent Ugp uptake system for glycerol-3-phosphate in Escherichia coli is trans inhibited by Pi. J Bacteriol. 1994;176:15-20 pubmed
    ..5 mM. Cells had to be starved for Pi in order to observe this inhibition. We conclude that the activity of the Ugp transport system is controlled by the level of internal phosphate. ..
  9. Schweizer H, Boos W. Characterization of the ugp region containing the genes for the phoB dependent sn-glycerol-3-phosphate transport system of Escherichia coli. Mol Gen Genet. 1984;197:161-8 pubmed
    ..are organized in two separate operons which comprise at least four genes: ugpB and ugpD constitute one operon, ugpA and ugpC constitute the other. The structural gene for the G3P binding protein (G3PBP) is ugpB...

More Information


  1. Schweizer H, Grussenmeyer T, Boos W. Mapping of two ugp genes coding for the pho regulon-dependent sn-glycerol-3-phosphate transport system of Escherichia coli. J Bacteriol. 1982;150:1164-71 pubmed
    Two genes, ugpA and ugpB, coding for a binding protein-dependent sn-glycerol-3-phosphate transport system, were mapped at 75.3 min on the Escherichia coli chromosome...
  2. Brzoska P, Boos W. Characteristics of a ugp-encoded and phoB-dependent glycerophosphoryl diester phosphodiesterase which is physically dependent on the ugp transport system of Escherichia coli. J Bacteriol. 1988;170:4125-35 pubmed
    ..The product of the ugpQ gene, expressed in minicells, has an apparent molecular weight of 17,500. We present evidence that only one major phoB-dependent promoter controls all ugp genes. ..
  3. Pritchard R, Ahmad S. Fluorouracil and the isolation of mutants lacking uridine phosphorylase in Escherichia coli: location of the gene. Mol Gen Genet. 1971;111:84-8 pubmed
  4. Hekstra D, Tommassen J. Functional exchangeability of the ABC proteins of the periplasmic binding protein-dependent transport systems Ugp and Mal of Escherichia coli. J Bacteriol. 1993;175:6546-52 pubmed
    ..that it was expressed at a sufficiently high level in the absence of the integral inner membrane components UgpA and/or UgpE of the Ugp system...
  5. Wuttge S, Bommer M, J├Ąger F, Martins B, Jacob S, Licht A, et al. Determinants of substrate specificity and biochemical properties of the sn-glycerol-3-phosphate ATP binding cassette transporter (UgpB-AEC2 ) of Escherichia coli. Mol Microbiol. 2012;86:908-20 pubmed publisher an ATP binding cassette importer composed of the periplasmic binding protein, UgpB, the transmembrane subunits, UgpA and UgpE, and a homodimer of the nucleotide binding subunit, UgpC...
  6. Xavier K, Kossmann M, Santos H, Boos W. Kinetic analysis by in vivo 31P nuclear magnetic resonance of internal Pi during the uptake of sn-glycerol-3-phosphate by the pho regulon-dependent Ugp system and the glp regulon-dependent GlpT system. J Bacteriol. 1995;177:699-704 pubmed
    ..Both systems were inversely controlled by internal Pi. Whereas the Ugp system was inhibited, the GlpT system was stimulated by elevated internal Pi. ..
  7. Overduin P, Boos W, Tommassen J. Nucleotide sequence of the ugp genes of Escherichia coli K-12: homology to the maltose system. Mol Microbiol. 1988;2:767-75 pubmed
    ..The sequences of the proteins contain the characteristics of several other binding protein-dependent transport systems, but they seem to be particularly closely related to the maltose system. ..
  8. Lemieux M, Huang Y, Wang D. The structural basis of substrate translocation by the Escherichia coli glycerol-3-phosphate transporter: a member of the major facilitator superfamily. Curr Opin Struct Biol. 2004;14:405-12 pubmed
    ..The structure and mechanism of the glycerol-3-phosphate transporter form a paradigm for other members of the major facilitator superfamily. ..
  9. Abramson J, Kaback H, Iwata S. Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily. Curr Opin Struct Biol. 2004;14:413-9 pubmed
    ..All three structures reveal 12 transmembrane helices forming two distinct domains, and could imply that members of this superfamily have preserved both secondary and tertiary structure elements during evolution. ..
  10. Ezaki B, Mori H, Ogura T, Hiraga S. Possible involvement of the ugpA gene product in the stable maintenance of mini-F plasmid in Escherichia coli. Mol Gen Genet. 1990;223:361-8 pubmed
    ..the seg-3 mutant carried two mutations that additively affected the maintenance of mini-F plasmid; one was in the ugpA gene and the other was presumably in the rpoH gene...