Gene Symbol: ubiE
Description: bifunctional 2-octaprenyl-6-methoxy-1,4-benzoquinone methylase/ S-adenosylmethionine:2-DMK methyltransferase
Alias: ECK3827, JW5581, menG, menH, yigO
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Alexander K, Young I. Alternative hydroxylases for the aerobic and anaerobic biosynthesis of ubiquinone in Escherichia coli. Biochemistry. 1978;17:4750-5 pubmed
    ..whereas mutants lacking the octaprenyltransferase (ubiA), carboxy-lyase (ubiD), or methyltransferases (ubiE or ubiG) of the aerobic pathway remain blocked anaerobically. The demonstration that E...
  2. Meganathan R. Ubiquinone biosynthesis in microorganisms. FEMS Microbiol Lett. 2001;203:131-9 pubmed
    ..While the prenyl side chain is formed by the 2-C-methyl-D-erythritol 4-phosphate (non-mevalonate) pathway in E. coli, it is formed by the mevalonate pathway in the yeast. ..
  3. Jiang M, Chen X, Guo Z, Cao Y, Chen M, Guo Z. Identification and characterization of (1R,6R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase in the menaquinone biosynthesis of Escherichia coli. Biochemistry. 2008;47:3426-34 pubmed publisher
    ..Here, we report the identification and characterization of MenH (or YfbB), an enzyme previously proposed to catalyze a late step in menaquinone biosynthesis, as the SHCHC synthase...
  4. Wissenbach U, Ternes D, Unden G. An Escherichia coli mutant containing only demethylmenaquinone, but no menaquinone: effects on fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate respiration. Arch Microbiol. 1992;158:68-73 pubmed
    The mutant strain AN70 (ubiE) of Escherichia coli which is known to lack ubiquinone (Young IG et al. 1971), was analyzed for menaquinone (MK) and demethylmenaquinone (DMK) contents...
  5. Tyson K, Metheringham R, Griffiths L, Cole J. Characterisation of Escherichia coli K-12 mutants defective in formate-dependent nitrite reduction: essential roles for hemN and the menFDBCE operon. Arch Microbiol. 1997;168:403-11 pubmed
    ..The effects of men, ubiA and ubiE mutations on other cytochrome-c-dependent electron transfer pathways were also determined...
  6. Meganathan R. Biosynthesis of menaquinone (vitamin K2) and ubiquinone (coenzyme Q): a perspective on enzymatic mechanisms. Vitam Horm. 2001;61:173-218 pubmed
    ..The current status of the pathways with particular emphasis on the reaction mechanisms, is discussed in this review. ..
  7. Poon W, Davis D, Ha H, Jonassen T, Rather P, Clarke C. Identification of Escherichia coli ubiB, a gene required for the first monooxygenase step in ubiquinone biosynthesis. J Bacteriol. 2000;182:5139-46 pubmed
    ..reveals no mutations within the ubiB gene, but instead shows the presence of an IS1 element at position +516 of the ubiE gene...
  8. Kouzminova E, Rotman E, Macomber L, Zhang J, Kuzminov A. RecA-dependent mutants in Escherichia coli reveal strategies to avoid chromosomal fragmentation. Proc Natl Acad Sci U S A. 2004;101:16262-7 pubmed
    ..The tdk and rdgB mutants affect synthesis of DNA precursors. The fur, ubiE, and ubiH mutants are likely to have increased levels of reactive oxygen species...
  9. Wallace B, Young I. Aerobic respiration in mutants of Escherichia coli accumulating quinone analogues of ubiquinone. Biochim Biophys Acta. 1977;461:75-83 pubmed
    ..differ from ubiquinone in terms of the substituents on the quinone ring, accumulate in the cytoplasmic membranes of ubiE-, ubiF- and ubiG- mutants...

More Information


  1. Young I, McCann L, Stroobant P, Gibson F. Characterization and genetic analysis of mutant strains of Escherichia coli K-12 accumulating the biquinone precursors 2-octaprenyl-6-methoxy-1,4-benzoquinone and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone. J Bacteriol. 1971;105:769-78 pubmed
    ..and 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinone were shown to carry mutations in genes designated ubiE and ubiF, respectively...
  2. Lee P, Hsu A, Ha H, Clarke C. A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene. J Bacteriol. 1997;179:1748-54 pubmed
    Strains of Escherichia coli with mutations in the ubiE gene are not able to catalyze the carbon methylation reaction in the biosynthesis of ubiquinone (coenzyme Q) and menaquinone (vitamin K2), essential isoprenoid quinone components of ..
  3. Bekker M, Kramer G, Hartog A, Wagner M, de Koster C, Hellingwerf K, et al. Changes in the redox state and composition of the quinone pool of Escherichia coli during aerobic batch-culture growth. Microbiology. 2007;153:1974-80 pubmed
    ..Moreover, in exponentially growing cultures in a well-shaken Erlenmeyer flask, it is observed that the assumption of a pseudo-steady state does not hold with respect to the redox state of the quinone pool. ..
  4. Lee K, Zhan X, Gao J, Qiu J, Feng Y, Meganathan R, et al. RraA. a protein inhibitor of RNase E activity that globally modulates RNA abundance in E. coli. Cell. 2003;114:623-34 pubmed
    ..Our results reveal a possible mechanism for the dynamic regulation of RNA decay and processing by inhibitory RNase binding proteins...
  5. Pacheco C, Passos J, Castro A, Moradas Ferreira P, De Marco P. Role of respiration and glutathione in cadmium-induced oxidative stress in Escherichia coli K-12. Arch Microbiol. 2008;189:271-8 pubmed