tus

Summary

Gene Symbol: tus
Description: inhibitor of replication at Ter, DNA-binding protein
Alias: ECK1605, JW1602, tau
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Hill T, Pelletier A, Tecklenburg M, Kuempel P. Identification of the DNA sequence from the E. coli terminus region that halts replication forks. Cell. 1988;55:459-66 pubmed
    ..contains two loci, T1 and T2, that inhibit the progress of replication forks and require the trans-acting factor tus. We have identified a 23 bp terminator signal at T1 and T2 that is within 100 bp of the sites of replication arrest...
  2. Nedved M, Gottlieb P, Moe G. CD and DNA binding studies of a proline repeat-containing segment of the replication arrest protein Tus. Nucleic Acids Res. 1994;22:5024-30 pubmed
    b>Tus is a sequence-specific DNA binding protein that regulates its own transcription and can arrest Escherichia coli replication when bound to Ter sites on the chromosome...
  3. Kamada K, Horiuchi T, Ohsumi K, Shimamoto N, Morikawa K. Structure of a replication-terminator protein complexed with DNA. Nature. 1996;383:598-603 pubmed
    The crystal structure of the Escherichia coli replication-terminator protein (Tus) bound to terminus-site (Ter) DNA has been determined at 2.7 A resolution...
  4. Wake R, King G. A tale of two terminators: crystal structures sharpen the debate on DNA replication fork arrest mechanisms. Structure. 1997;5:1-5 pubmed
    The structure of the Tus-Ter DNA replication fork arrest complex of Escherichia coli reveals a novel architecture for the bound Tus protein and a new type of DNA-binding motif...
  5. Andersen P, Griffiths A, Duggin I, Wake R. Functional specificity of the replication fork-arrest complexes of Bacillus subtilis and Escherichia coli: significant specificity for Tus-Ter functioning in E. coli. Mol Microbiol. 2000;36:1327-35 pubmed
    ..After transferring these new plasmids into B. subtilis, which could overproduce the E. coli terminator protein Tus, it was shown that the E...
  6. Gottlieb P, Wu S, Zhang X, Tecklenburg M, Kuempel P, Hill T. Equilibrium, kinetic, and footprinting studies of the Tus-Ter protein-DNA interaction. J Biol Chem. 1992;267:7434-43 pubmed
    ..in the terminus region of the Escherichia coli chromosome is mediated by protein-DNA complexes composed of the Tus protein and 23 base pair sequences generically called Ter sites...
  7. Duggin I, Bell S. Termination structures in the Escherichia coli chromosome replication fork trap. J Mol Biol. 2009;387:532-9 pubmed publisher
    ..Quantification of fork pausing at the Ter sites in both their native chromosomal context and the plasmid context further supported the fork trap model. ..
  8. Duval Valentin G, Marty Cointin B, Chandler M. Requirement of IS911 replication before integration defines a new bacterial transposition pathway. EMBO J. 2004;23:3897-906 pubmed
    ..the replicative mechanism, we have also used the Escherichia coli terminators (terC) which, when bound by the Tus protein, inhibit replication forks in a polarised manner...
  9. Natarajan S, Kelley W, Bastia D. Replication terminator protein of Escherichia coli is a transcriptional repressor of its own synthesis. Proc Natl Acad Sci U S A. 1991;88:3867-71 pubmed
    ..Analysis of the tus gene that encodes the Ter protein revealed that transcription was initiated from a single promoter located within ..

More Information

Publications60

  1. Henderson T, Nilles A, Valjavec Gratian M, Hill T. Site-directed mutagenesis and phylogenetic comparisons of the Escherichia coli Tus protein: DNA-protein interactions alone can not account for Tus activity. Mol Genet Genomics. 2001;265:941-53 pubmed
    The Tus protein of Escherichia coli is capable of arresting DNA replication in an orientation-dependent manner when bound to specific sequences in the bacterial chromosome called Ter sites...
  2. Khatri G, MacAllister T, Sista P, Bastia D. The replication terminator protein of E. coli is a DNA sequence-specific contra-helicase. Cell. 1989;59:667-74 pubmed
    We have cloned the tus gene that encodes the replication terminator protein of Escherichia coli and have efficiently expressed its gene product...
  3. Kaplan D, Bastia D. Mechanisms of polar arrest of a replication fork. Mol Microbiol. 2009;72:279-85 pubmed publisher
    ..In yeast, terminator sequences are present in rDNA non-transcribed spacers and a region immediately preceding the mating type switch locus Mat1, and the mechanism of polar arrest at these regions is beginning to be elucidated. ..
  4. Coskun Ari F, Skokotas A, Moe G, Hill T. Biophysical characteristics of Tus, the replication arrest protein of Escherichia coli. J Biol Chem. 1994;269:4027-34 pubmed
    b>Tus, a DNA-binding protein, mediates arrest of DNA replication in Escherichia coli...
  5. Bastia D, Zzaman S, Krings G, Saxena M, Peng X, Greenberg M. Replication termination mechanism as revealed by Tus-mediated polar arrest of a sliding helicase. Proc Natl Acad Sci U S A. 2008;105:12831-6 pubmed publisher
    The replication terminator protein Tus of Escherichia coli promotes polar fork arrest at sequence-specific replication termini (Ter) by antagonizing DNA unwinding by the replicative helicase DnaB...
  6. Guajardo R, Sousa R. Characterization of the effects of Escherichia coli replication terminator protein (Tus) on transcription reveals dynamic nature of the tus block to transcription complex progression. Nucleic Acids Res. 1999;27:2814-24 pubmed
    We have characterized the blocks to progression of T7 and T3 RNA polymerase transcription complexes created when a Tus protein is bound to the template...
  7. Pelletier A, Hill T, Kuempel P. Location of sites that inhibit progression of replication forks in the terminus region of Escherichia coli. J Bacteriol. 1988;170:4293-8 pubmed
    ..Inhibition at T1 was not detectable in our assay in strains lacking the trans-acting locus tus, which is located near T2 and is required for T1 to function...
  8. Neylon C, Brown S, Kralicek A, Miles C, Love C, Dixon N. Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA-binding studies of mutant proteins by surface plasmon resonance. Biochemistry. 2000;39:11989-99 pubmed
    The Escherichia coli replication terminator protein (Tus) binds tightly and specifically to termination sites such as TerB in order to halt DNA replication...
  9. Lee E, Kornberg A, Hidaka M, Kobayashi T, Horiuchi T. Escherichia coli replication termination protein impedes the action of helicases. Proc Natl Acad Sci U S A. 1989;86:9104-8 pubmed
    ..Thus, the intrinsic nature of the oriented TBP-ter complex is responsible for impeding the helicases, rather than any protein-protein interactions. ..
  10. Kobayashi T, Hidaka M, Horiuchi T. Evidence of a ter specific binding protein essential for the termination reaction of DNA replication in Escherichia coli. EMBO J. 1989;8:2435-41 pubmed
    ..and subsequent replacement of the corresponding chromosomal region with the resulting mutant alleles, we isolated tau- mutants completely defective in ter binding activity...
  11. Sharma B, Hill T. TerF, the sixth identified replication arrest site in Escherichia coli, is located within the rcsC gene. J Bacteriol. 1992;174:7854-8 pubmed
    ..demonstrate that the TerF site is functional in both chromosomal and plasmid environments and that the stability of the Tus-TerF protein-DNA complex more closely resembles the plasmid R6K Ter sites than the chromosomal TerB site.
  12. Hill T, Tecklenburg M, Pelletier A, Kuempel P. tus, the trans-acting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein. Proc Natl Acad Sci U S A. 1989;86:1593-7 pubmed
    ..of DNA replication in Escherichia coli include the terminator signals T1 and T2 and the trans-acting gene tus. We have shown previously that tus maps in a 4-kilobase region of the chromosomal terminus near T2...
  13. Skokotas A, Hiasa H, Marians K, O DONNELL L, Hill T. Mutations in the Escherichia coli Tus protein define a domain positioned close to the DNA in the Tus-Ter complex. J Biol Chem. 1995;270:30941-8 pubmed
    A new genetic screen for mutations in the tus gene of Escherichia coli has been devised that selects for Tus proteins with altered ability to arrest DNA replication...
  14. Moolman M, Tiruvadi Krishnan S, Kerssemakers J, de Leeuw R, Lorent V, Sherratt D, et al. The progression of replication forks at natural replication barriers in live bacteria. Nucleic Acids Res. 2016;44:6262-73 pubmed publisher
    ..One such barrier is the Tus-ter complex, which is a direction dependent barrier for replication fork progression...
  15. Skokotas A, Wrobleski M, Hill T. Isolation and characterization of mutants of Tus, the replication arrest protein of Escherichia coli. J Biol Chem. 1994;269:20446-55 pubmed
    Mutations in the tus gene of Escherichia coli, which encodes the replication arrest protein Tus, were isolated using a selection scheme based on the plasmid pHV750T2+, which transforms tus mutants at a much higher frequency than wild ..
  16. Lee S, Xie A, Jiang W, Etchegaray J, Jones P, Inouye M. Family of the major cold-shock protein, CspA (CS7.4), of Escherichia coli, whose members show a high sequence similarity with the eukaryotic Y-box binding proteins. Mol Microbiol. 1994;11:833-9 pubmed
    ..A similar cold-shock induction to cspA was observed for cspB but not cspC and cspD. These results indicate that E. coli has a family of the cspA gene, some of which are induced by cold shock. ..
  17. Pandey M, Elshenawy M, Jergic S, Takahashi M, Dixon N, Hamdan S, et al. Two mechanisms coordinate replication termination by the Escherichia coli Tus-Ter complex. Nucleic Acids Res. 2015;43:5924-35 pubmed publisher
    The Escherichia coli replication terminator protein (Tus) binds to Ter sequences to block replication forks approaching from one direction...
  18. DasGupta S, Bernander R, Nordstrom K. In vivo effect of the tus mutation on cell division in an Escherichia coli strain where chromosome replication is under the control of plasmid R1. Res Microbiol. 1991;142:177-80 pubmed
    The phenotypic effect of the tus::kan mutation in an Escherichia coli strain, where the chromosome is replicated unidirectionally by an integrated R1 miniplasmid, was examined by flow cytometry and phase fluorescence microscopy...
  19. Maisnier Patin S, Nordstrom K, DasGupta S. Replication arrests during a single round of replication of the Escherichia coli chromosome in the absence of DnaC activity. Mol Microbiol. 2001;42:1371-82 pubmed
    ..The forks at the terminal Ter sites disappeared in the absence of Tus protein, as the active forks could then pass through the terminus to reach the arrest site, and the unfinished ..
  20. Sahoo T, Mohanty B, Lobert M, Manna A, Bastia D. The contrahelicase activities of the replication terminator proteins of Escherichia coli and Bacillus subtilis are helicase-specific and impede both helicase translocation and authentic DNA unwinding. J Biol Chem. 1995;270:29138-44 pubmed
    ..These results are significant because of their relevance to the mechanistic aspects of replication termination. ..
  21. Woods S, Miles J, Roberts R, Guest J. Structural and functional relationships between fumarase and aspartase. Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12. Biochem J. 1986;237:547-57 pubmed
    ..coli W. Remarkable homologies were found between the primary structures of the fumarase (fumC and citG) and aspartase (aspA) genes and their products, suggesting close structural and evolutionary relationships. ..
  22. Zhou P, Bogan J, Welch K, Pickett S, Wang H, Zaritsky A, et al. Gene transcription and chromosome replication in Escherichia coli. J Bacteriol. 1997;179:163-9 pubmed
    ..The transcriptional levels for two genes, minE and tus, did not fluctuate significantly, whereas the transcripts for one gene, iciA, appeared to increase transiently...
  23. Bidnenko V, Lestini R, Michel B. The Escherichia coli UvrD helicase is essential for Tus removal during recombination-dependent replication restart from Ter sites. Mol Microbiol. 2006;62:382-96 pubmed
    ..We propose that UvrD is required for Tus removal from Ter sites...
  24. Hiasa H, Marians K. Tus prevents overreplication of oriC plasmid DNA. J Biol Chem. 1994;269:26959-68 pubmed
    ..and two Ter sites, oriented as they are on the Escherichia coli chromosome, have been used to study the role of Tus in termination of bidirectional replication...
  25. Bierne H, Ehrlich S, Michel B. Flanking sequences affect replication arrest at the Escherichia coli terminator TerB in vivo. J Bacteriol. 1994;176:4165-7 pubmed
    We have analyzed the effect of flanking sequences on Tus-induced replication arrest. pBR322 plasmid derivatives which carry the Escherichia coli replication terminator TerB at different locations were used...
  26. Souter S, Lee G. Microtubule-associated protein tau in human prostate cancer cells: isoforms, phosphorylation, and interactions. J Cell Biochem. 2009;108:555-64 pubmed publisher
    b>Tau is a microtubule-associated protein whose function has been investigated primarily in neurons. Recently, tau expression has been correlated with increased drug resistance in various cancers of non-neuronal tissues...
  27. Dahdah D, Morin I, Moreau M, Dixon N, Schaeffer P. Site-specific covalent attachment of DNA to proteins using a photoactivatable Tus-Ter complex. Chem Commun (Camb). 2009;:3050-2 pubmed publisher
    Investigations into the photocrosslinking kinetics of the protein Tus with various bromodeoxyuridine-substituted Ter DNA variants highlight the potential use of this complex as a photoactivatable connector between proteins of interest ..
  28. Hill T, Kopp B, Kuempel P. Termination of DNA replication in Escherichia coli requires a trans-acting factor. J Bacteriol. 1988;170:662-8 pubmed
    ..We have called this newly identified terminus function the termination utilization substance (tus).
  29. Kamada K, Ohsumi K, Horiuchi T, Shimamoto N, Morikawa K. Crystallization and preliminary X-ray analysis of the Escherichia coli replication terminator protein complexed with DNA. Proteins. 1996;24:402-3 pubmed
    Crystals of the Escherichia coli replication terminator protein (Tus) complexed with its binding site DNA were obtained by a microdialysis method using PEG 4000...
  30. Duggan L, Hill T, Wu S, Garrison K, Zhang X, Gottlieb P. Using modified nucleotides to map the DNA determinants of the Tus-TerB complex, the protein-DNA interaction associated with termination of replication in Escherichia coli. J Biol Chem. 1995;270:28049-54 pubmed
    ..of modified nucleotides was used to map the hydrogen-bonding and hydrophobic sites of the TerB DNA required for Tus interaction...
  31. Krabbe M, Zabielski J, Bernander R, Nordstrom K. Inactivation of the replication-termination system affects the replication mode and causes unstable maintenance of plasmid R1. Mol Microbiol. 1997;24:723-35 pubmed
    Two so-called Ter sites, which bind the Escherichia coli Tus protein, are located near the replication origin of plasmid R1...
  32. Francois V, Louarn J, Patte J, Rebollo J, Louarn J. Constraints in chromosomal inversions in Escherichia coli are not explained by replication pausing at inverted terminator-like sequences. Mol Microbiol. 1990;4:537-42 pubmed
    ..A mutation in the tus gene is known to abolish replication pausing at terminators...
  33. Mulcair M, Schaeffer P, Oakley A, Cross H, Neylon C, Hill T, et al. A molecular mousetrap determines polarity of termination of DNA replication in E. coli. Cell. 2006;125:1309-19 pubmed
    During chromosome synthesis in Escherichia coli, replication forks are blocked by Tus bound Ter sites on approach from one direction but not the other...
  34. Hill T, Marians K. Escherichia coli Tus protein acts to arrest the progression of DNA replication forks in vitro. Proc Natl Acad Sci U S A. 1990;87:2481-5 pubmed
    A polar DNA replication barrier is formed when the DNA-binding protein Tus forms a complex with any of the four 23-base-pair terminator (ter) sites found in the terminus region of the Escherichia coli chromosome...
  35. Guzmán E, Caballero J, Jimenez Sanchez A. Ribonucleoside diphosphate reductase is a component of the replication hyperstructure in Escherichia coli. Mol Microbiol. 2002;43:487-95 pubmed
    ..The absence of Tus protein, which specifically stops the replication complex by inhibiting replicative helicase activity, allows forks ..
  36. Roecklein B, Kuempel P. In vivo characterization of tus gene expression in Escherichia coli. Mol Microbiol. 1992;6:1655-61 pubmed
    The tus gene encodes a DNA-binding protein (Tus) that inhibits replication forks at specific block-sites within the terminus region of the Escherichia coli chromosome. One of these block-sites, TerB, is adjacent to the tus gene...
  37. Markovitz A. A new in vivo termination function for DNA polymerase I of Escherichia coli K12. Mol Microbiol. 2005;55:1867-82 pubmed
    Escherichia coli deleted for the tus gene are viable. Thus there must be at least one other mechanism for terminating chromosome synthesis...
  38. Schaeffer P, Headlam M, Dixon N. Protein--protein interactions in the eubacterial replisome. IUBMB Life. 2005;57:5-12 pubmed
    ..fork that arrives first in the terminus region is halted by its encounter with a correctly-oriented complex of the Tus replication terminator protein bound at one of several Ter sites, where it is trapped until the other fork arrives...
  39. Hiasa H, Marians K. Differential inhibition of the DNA translocation and DNA unwinding activities of DNA helicases by the Escherichia coli Tus protein. J Biol Chem. 1992;267:11379-85 pubmed
    Binding of the Escherichia coli Tus protein to its cognate nonpalindromic binding site on duplex DNA (a Ter sequence) is sufficient to arrest the progression of replication forks in a Ter orientation-dependent manner in vivo and in vitro...
  40. Valjavec Gratian M, Henderson T, Hill T. Tus-mediated arrest of DNA replication in Escherichia coli is modulated by DNA supercoiling. Mol Microbiol. 2005;58:758-73 pubmed
    In the absence of RecA, expression of the Tus protein of Escherichia coli is lethal when ectopic Ter sites are inserted into the chromosome in an orientation that blocks completion of chromosome replication...
  41. Kapur A, Beck J, Brown S, Dixon N, Sheil M. Use of electrospray ionization mass spectrometry to study binding interactions between a replication terminator protein and DNA. Protein Sci. 2002;11:147-57 pubmed
    b>Tus protein binds tightly to specific DNA sequences (Ter) on the Escherichia coli chromosome halting replication. We report here conditions for detecting the 1 : 1 Tus-Ter complex by electrospray ionization mass spectrometry (ESI-MS)...
  42. Mulugu S, Potnis A, Shamsuzzaman -, Taylor J, Alexander K, Bastia D. Mechanism of termination of DNA replication of Escherichia coli involves helicase-contrahelicase interaction. Proc Natl Acad Sci U S A. 2001;98:9569-74 pubmed
    Using yeast forward and reverse two-hybrid analyses, we have discovered that the replication terminator protein Tus of Escherichia coli physically interacts with DnaB helicase in vivo...
  43. Coskun Ari F, Hill T. Sequence-specific interactions in the Tus-Ter complex and the effect of base pair substitutions on arrest of DNA replication in Escherichia coli. J Biol Chem. 1997;272:26448-56 pubmed
    Arrest of DNA replication in Escherichia coli is mediated by specific interactions between the Tus protein and terminator (Ter) sequences...
  44. Pelletier A, Hill T, Kuempel P. Termination sites T1 and T2 from the Escherichia coli chromosome inhibit DNA replication in ColE1-derived plasmids. J Bacteriol. 1989;171:1739-41 pubmed
    ..These characteristics included polarity and dependence on tus, a trans-acting factor required for inhibition...
  45. Roecklein B, Pelletier A, Kuempel P. The tus gene of Escherichia coli: autoregulation, analysis of flanking sequences and identification of a complementary system in Salmonella typhimurium. Res Microbiol. 1991;142:169-75 pubmed
    The tus gene of Escherichia coli encodes a DNA-binding protein that, when bound to terminator sites, blocks replication forks...
  46. Neylon C, Kralicek A, Hill T, Dixon N. Replication termination in Escherichia coli: structure and antihelicase activity of the Tus-Ter complex. Microbiol Mol Biol Rev. 2005;69:501-26 pubmed
    The arrest of DNA replication in Escherichia coli is triggered by the encounter of a replisome with a Tus protein-Ter DNA complex...
  47. Kaplan D. Replication termination: mechanism of polar arrest revealed. Curr Biol. 2006;16:R684-6 pubmed
    The Tus-Ter protein-DNA complex of Escherichia coli blocks progression of DNA replication from only one direction at the replication terminus...
  48. Butcher D, Nedved M, Neiss T, Moe G. Proline pipe helix: structure of the tus proline repeat determined by 1H NMR. Biochemistry. 1996;35:698-703 pubmed
    ..22, 5024-5030], that is similar to the proline repeat segment of the replication arrest protein, Tus, has been determined by 1H NMR in 50% trifluroethanol. The structure is a novel left-handed helix having 5...
  49. Hidaka M, Kobayashi T, Takenaka S, Takeya H, Horiuchi T. Purification of a DNA replication terminus (ter) site-binding protein in Escherichia coli and identification of the structural gene. J Biol Chem. 1989;264:21031-7 pubmed
    ..We reported that extract of the cells carrying the plasmid with the tau gene, which was identified to be an essential gene for the termination reaction at the ter site, contained about an ..
  50. Kuempel P, Pelletier A, Hill T. Tus and the terminators: the arrest of replication in prokaryotes. Cell. 1989;59:581-3 pubmed
  51. Lee E, Kornberg A. Features of replication fork blockage by the Escherichia coli terminus-binding protein. J Biol Chem. 1992;267:8778-84 pubmed
    ..The ter sequences of plasmids R1-terR and -terL and of plasmids R6K and R100 have been compared with those of E. coli-terA and -terB. ..