Gene Symbol: tsx
Description: nucleoside channel, receptor of phage T6 and colicin K
Alias: ECK0405, JW0401, T6rec, nupA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Lucht J, Boos W, Bremer E. Alignment of genes from the 9-minute region (araJ to tsx) of the Escherichia coli K-12 linkage map to the physical map. J Bacteriol. 1992;174:1709-10 pubmed
  2. Manning P, Reeves P. Outer membrane of Escherichia coli K-12: TSX mutants (resistant to bacteriophage T6 and colicin K) lack an outer membrane protein. Biochem Biophys Res Commun. 1976;71:466-71 pubmed
  3. Hantke K. Phage T6--colicin K receptor and nucleoside transport in Escherichia coli. FEBS Lett. 1976;70:109-12 pubmed
  4. Bremer E, Middendorf A, Martinussen J, Valentin Hansen P. Analysis of the tsx gene, which encodes a nucleoside-specific channel-forming protein (Tsx) in the outer membrane of Escherichia coli. Gene. 1990;96:59-65 pubmed
    The tsx gene of Escherichia coli encodes an outer membrane protein, Tsx, which constitutes the receptor for colicin K and bacteriophage T6, and functions as a substrate-specific channel for nucleosides and deoxynucleosides...
  5. Benz R, Schmid A, Maier C, Bremer E. Characterization of the nucleoside-binding site inside the Tsx channel of Escherichia coli outer membrane. Reconstitution experiments with lipid bilayer membranes. Eur J Biochem. 1988;176:699-705 pubmed
    Reconstitution of purified Tsx protein from Escherichia coli into lipid bilayer membranes showed that Tsx formed small ion-permeable channels with a single-channel conductance of 10 pS in 1 M KCl...
  6. Bremer E, Gerlach P, Middendorf A. Double negative and positive control of tsx expression in Escherichia coli. J Bacteriol. 1988;170:108-16 pubmed
    The Escherichia coli tsx gene encodes an outer membrane protein that is involved in nucleoside uptake and serves as the receptor protein for colicin K and several bacteriophages...
  7. Maier C, Bremer E, Schmid A, Benz R. Pore-forming activity of the Tsx protein from the outer membrane of Escherichia coli. Demonstration of a nucleoside-specific binding site. J Biol Chem. 1988;263:2493-9 pubmed
    The Tsx protein from the outer membrane of Escherichia coli is known to be involved in the permeation of nucleosides across the outer membrane under limiting substrate conditions. We purified Tsx from an E...
  8. Pugsley A. Escherichia coli K12 strains for use in the identification and characterization of colicins. J Gen Microbiol. 1985;131:369-76 pubmed
    ..The strains can be used in typing schemes based on the identification of colicins, in the detection of new types of colicins/microcins, and in the further characterization of previously identified colicins/microcins and their plasmids. ..
  9. Xu C, Lin X, Ren H, Zhang Y, Wang S, Peng X. Analysis of outer membrane proteome of Escherichia coli related to resistance to ampicillin and tetracycline. Proteomics. 2006;6:462-73 pubmed
    ..proteins, 3 were known as antibiotic-resistant proteins, including TolC, OmpC and YhiU, while FimD precursor, LamB, Tsx, YfiO, OmpW, NlpB were first reported here to be antibiotic-resistance-related proteins...

More Information


  1. Ye J, van den Berg B. Crystal structure of the bacterial nucleoside transporter Tsx. EMBO J. 2004;23:3187-95 pubmed
    b>Tsx is a nucleoside-specific outer membrane (OM) transporter of Gram-negative bacteria. We present crystal structures of Escherichia coli Tsx in the absence and presence of nucleosides...
  2. Ranquin A, van Gelder P. Maltoporin: sugar for physics and biology. Res Microbiol. 2004;155:611-6 pubmed
    ..This trimeric bacterial outer membrane channel allows permeation of sugars such as maltodextrins. Its structure is described and functional studies resulting in a mechanistic transport model are critically discussed. ..
  3. Wu L, Lin X, Peng X. From proteome to genome for functional characterization of pH-dependent outer membrane proteins in Escherichia coli. J Proteome Res. 2009;8:1059-70 pubmed publisher
    ..Among the differentially expressed proteins identified, OstA, TolC, OmpT, OmpP OmpC, Trak, OmpX, Dps, LamB, Tsx, FadL, OmpW, and OmpF were characterized as pH-related OM proteins...
  4. Zhang D, Jiang B, Xiang Z, Wang S. Functional characterisation of altered outer membrane proteins for tetracycline resistance in Escherichia coli. Int J Antimicrob Agents. 2008;32:315-9 pubmed publisher
    ..In this study, upregulation of FimD, Tsx, OmpW, OmpC and TolC and downregulation of LamB in response to tetracycline were detected and confirmed by two-..
  5. Klammt C, Schwarz D, Fendler K, Haase W, Dötsch V, Bernhard F. Evaluation of detergents for the soluble expression of alpha-helical and beta-barrel-type integral membrane proteins by a preparative scale individual cell-free expression system. FEBS J. 2005;272:6024-38 pubmed
    ..proteins, namely the bacterial alpha-helical multidrug transporter, EmrE, the beta-barrel nucleoside transporter, Tsx, and the porcine vasopressin receptor of the eukaryotic superfamily of G-protein coupled receptors...
  6. Acimovic Y, Coe I. Molecular evolution of the equilibrative nucleoside transporter family: identification of novel family members in prokaryotes and eukaryotes. Mol Biol Evol. 2002;19:2199-210 pubmed
    ..Finally, we propose an evolutionary history for the ENT family which clarifies the origin(s) of multiple isoforms in different taxa. ..
  7. Schneider H, Fsihi H, Kottwitz B, Mygind B, Bremer E. Identification of a segment of the Escherichia coli Tsx protein that functions as a bacteriophage receptor area. J Bacteriol. 1993;175:2809-17 pubmed
    The Escherichia coli outer membrane protein Tsx functions as a nucleoside-specific channel and serves as the receptor for colicin K and a number of T-even-type bacteriophages, including phage T6...
  8. Fsihi H, Kottwitz B, Bremer E. Single amino acid substitutions affecting the substrate specificity of the Escherichia coli K-12 nucleoside-specific Tsx channel. J Biol Chem. 1993;268:17495-503 pubmed
    The Tsx protein from the Escherichia coli outer membrane is a channel-forming protein containing a nucleoside-specific binding site. The antibiotic albicidin enters the cell via this substrate-specific channel...
  9. Birch R, Pemberton J, Basnayake W. Stable albicidin resistance in Escherichia coli involves an altered outer-membrane nucleoside uptake system. J Gen Microbiol. 1990;136:51-8 pubmed
    ..coli simultaneously became resistant to phage T6. The locus determining albicidin sensitivity mapped at tsx, the structural gene for an outer-membrane protein used as a receptor by phage T6 and involved in transport through ..