Gene Symbol: truB
Description: tRNA pseudouridine synthase B: tRNA pseudouridine(55) synthase and putative tmRNA pseudouridine(342) synthase
Alias: ECK3155, JW3135, yhbA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Keffer Wilkes L, Veerareddygari G, Kothe U. RNA modification enzyme TruB is a tRNA chaperone. Proc Natl Acad Sci U S A. 2016;113:14306-14311 pubmed
    ..unclear, such as for pseudouridine formation in the tRNA TΨC arm by the bacterial tRNA pseudouridine synthase TruB. Here we test the hypothesis that RNA modification enzymes also act as RNA chaperones...
  2. Hamma T, Ferré D Amaré A. Pseudouridine synthases. Chem Biol. 2006;13:1125-35 pubmed
  3. Urbonavicius J, Durand J, Bj rk G. Three modifications in the D and T arms of tRNA influence translation in Escherichia coli and expression of virulence genes in Shigella flexneri. J Bacteriol. 2002;184:5348-57 pubmed
    ..The formation of these modified nucleosides is catalyzed by the products of genes trmH (Gm18), trmA (m(5)U54), and truB (Psi55)...
  4. Urbonavicius J, Stahl G, Durand J, Ben Salem S, Qian Q, Farabaugh P, et al. Transfer RNA modifications that alter +1 frameshifting in general fail to affect -1 frameshifting. RNA. 2003;9:760-8 pubmed
    Using mutants (tgt, mnmA(asuE, trmU), mnmE(trmE), miaA, miaB, miaE, truA(hisT), truB) of either Escherichia coli or Salmonella enterica serovar Typhimurium and the trm5 mutant of Saccharomyces cerevisiae, we have analyzed the influence by ..
  5. Wright J, Keffer Wilkes L, Dobing S, Kothe U. Pre-steady-state kinetic analysis of the three Escherichia coli pseudouridine synthases TruB, TruA, and RluA reveals uniformly slow catalysis. RNA. 2011;17:2074-84 pubmed publisher
    ..A novel stopped-flow absorbance assay revealed that substrate tRNA binding by TruB takes place in two steps with an overall rate of 6 sec(-1)...
  6. Hamilton C, Spedaliere C, Ginter J, Johnston M, Mueller E. The roles of the essential Asp-48 and highly conserved His-43 elucidated by the pH dependence of the pseudouridine synthase TruB. Arch Biochem Biophys. 2005;433:322-34 pubmed
    ..synthases have a conserved aspartic acid residue that is essential for catalysis, Asp-48 in Escherichia coli TruB. To probe the role of this residue, inactive D48C TruB was oxidized to generate the sulfinic acid cognate of ..
  7. Spedaliere C, Ginter J, Johnston M, Mueller E. The pseudouridine synthases: revisiting a mechanism that seemed settled. J Am Chem Soc. 2004;126:12758-9 pubmed
    ..studies with [18O]water are now reported that rule out such ester hydrolysis in one pseudouridine synthase, TruB. The aspartic acid residue does not become labeled, and the hydroxyl group in the hydrated product of f 5U derives ..
  8. Pan H, Agarwalla S, Moustakas D, Finer Moore J, Stroud R. Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit. Proc Natl Acad Sci U S A. 2003;100:12648-53 pubmed publisher
    RNA pseudouridine synthase, TruB, catalyzes pseudouridine formation at U55 in tRNA. This posttranscriptional modification is almost universally conserved and occurs in the T arm of most tRNAs...
  9. Sands J, Regnier P, Cummings H, Grunberg Manago M, Hershey J. The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping. Nucleic Acids Res. 1988;16:10803-16 pubmed
    ..The results indicate that P15B and P35 are expressed as part of the metY operon, but that some transcriptional read through into the rpsO operon also occurs, thereby, functionally linking the expression of these two complex systems. ..

More Information


  1. Kinghorn S, O Byrne C, Booth I, Stansfield I. Physiological analysis of the role of truB in Escherichia coli: a role for tRNA modification in extreme temperature resistance. Microbiology. 2002;148:3511-20 pubmed publisher
    The truB gene of Escherichia coli encodes the pseudouridine-55 (psi55) synthase and is responsible for modifying all tRNA molecules in the cell at the U55 position...
  2. Nurse K, Wrzesinski J, Bakin A, Lane B, Ofengand J. Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli. RNA. 1995;1:102-12 pubmed
    ..86 min on the E. coli chromosome between the infB and rpsO genes. The proposed name for this gene is truB. There is very little protein sequence homology between the truB gene product and the hisT (truA) product, which ..
  3. Hoang C, Ferr D Amar A. Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme. Cell. 2001;107:929-39 pubmed
    ..b>TruB is responsible for the Psi residue present in the T loops of virtually all tRNAs...
  4. Gutgsell N, Englund N, Niu L, Kaya Y, Lane B, Ofengand J. Deletion of the Escherichia coli pseudouridine synthase gene truB blocks formation of pseudouridine 55 in tRNA in vivo, does not affect exponential growth, but confers a strong selective disadvantage in competition with wild-type cells. RNA. 2000;6:1870-81 pubmed
    Previous work from this laboratory (Nurse et al., RNA, 1995, 1:102-112) established that TruB, a pseudouridine (psi) synthase from Escherichia coli, was able to make psi55 in tRNA transcripts but not in transcripts of full-length or ..
  5. Spedaliere C, Mueller E. Not all pseudouridine synthases are potently inhibited by RNA containing 5-fluorouridine. RNA. 2004;10:192-9 pubmed
    ..of pseudouridine synthases by RNA containing 5-fluorouridine, the Escherichia coli pseudouridine synthase TruB, which has physiologically critical eukaryotic homologs, is not inhibited by such RNA...
  6. Spedaliere C, Hamilton C, Mueller E. Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues. Biochemistry. 2000;39:9459-65 pubmed
    ..of these alignments by substituting the nearly invariant lysine and proline residues in Motif I of RluA and TruB, pseudouridine synthases belonging to different families...
  7. Ramamurthy V, Swann S, Spedaliere C, Mueller E. Role of cysteine residues in pseudouridine synthases of different families. Biochemistry. 1999;38:13106-11 pubmed
    ..We examined the enzymes RluA and TruB, which are members of different families than TruA and each other...
  8. Hoang C, Hamilton C, Mueller E, Ferré D Amaré A. Precursor complex structure of pseudouridine synthase TruB suggests coupling of active site perturbations to an RNA-sequestering peripheral protein domain. Protein Sci. 2005;14:2201-6 pubmed
    The pseudouridine synthase TruB is responsible for the universally conserved post-transcriptional modification of residue 55 of elongator tRNAs...
  9. Ramamurthy V, Swann S, Paulson J, Spedaliere C, Mueller E. Critical aspartic acid residues in pseudouridine synthases. J Biol Chem. 1999;274:22225-30 pubmed
  10. Phannachet K, Elias Y, Huang R. Dissecting the roles of a strictly conserved tyrosine in substrate recognition and catalysis by pseudouridine 55 synthase. Biochemistry. 2005;44:15488-94 pubmed publisher
    Sequence alignment of the TruA, TruB, RsuA, and RluA families of pseudouridine synthases (PsiS) identifies a strictly conserved aspartic acid, which has been shown to be the critical nucleophile for the PsiS-catalyzed formation of ..
  11. Hur S, Stroud R, Finer Moore J. Substrate recognition by RNA 5-methyluridine methyltransferases and pseudouridine synthases: a structural perspective. J Biol Chem. 2006;281:38969-73 pubmed
  12. Nakamura Y, Mizusawa S. In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins. EMBO J. 1985;4:527-32 pubmed
    ..The in vivo attenuation site of this operon is described. ..