Gene Symbol: truB
Description: tRNA pseudouridine synthase B
Species: Escherichia coli O157:H7 str. Sakai

Top Publications

  1. Sands J, Regnier P, Cummings H, Grunberg Manago M, Hershey J. The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping. Nucleic Acids Res. 1988;16:10803-16 pubmed
    ..The results indicate that P15B and P35 are expressed as part of the metY operon, but that some transcriptional read through into the rpsO operon also occurs, thereby, functionally linking the expression of these two complex systems. ..
  2. Ramamurthy V, Swann S, Spedaliere C, Mueller E. Role of cysteine residues in pseudouridine synthases of different families. Biochemistry. 1999;38:13106-11 pubmed
    ..We examined the enzymes RluA and TruB, which are members of different families than TruA and each other...
  3. Gutgsell N, Englund N, Niu L, Kaya Y, Lane B, Ofengand J. Deletion of the Escherichia coli pseudouridine synthase gene truB blocks formation of pseudouridine 55 in tRNA in vivo, does not affect exponential growth, but confers a strong selective disadvantage in competition with wild-type cells. RNA. 2000;6:1870-81 pubmed
    Previous work from this laboratory (Nurse et al., RNA, 1995, 1:102-112) established that TruB, a pseudouridine (psi) synthase from Escherichia coli, was able to make psi55 in tRNA transcripts but not in transcripts of full-length or ..
  4. Hoang C, Ferr D Amar A. Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme. Cell. 2001;107:929-39 pubmed
    ..b>TruB is responsible for the Psi residue present in the T loops of virtually all tRNAs...
  5. Kinghorn S, O Byrne C, Booth I, Stansfield I. Physiological analysis of the role of truB in Escherichia coli: a role for tRNA modification in extreme temperature resistance. Microbiology. 2002;148:3511-20 pubmed publisher
    The truB gene of Escherichia coli encodes the pseudouridine-55 (psi55) synthase and is responsible for modifying all tRNA molecules in the cell at the U55 position...
  6. Urbonavicius J, Stahl G, Durand J, Ben Salem S, Qian Q, Farabaugh P, et al. Transfer RNA modifications that alter +1 frameshifting in general fail to affect -1 frameshifting. RNA. 2003;9:760-8 pubmed
    Using mutants (tgt, mnmA(asuE, trmU), mnmE(trmE), miaA, miaB, miaE, truA(hisT), truB) of either Escherichia coli or Salmonella enterica serovar Typhimurium and the trm5 mutant of Saccharomyces cerevisiae, we have analyzed the influence by ..
  7. Pan H, Agarwalla S, Moustakas D, Finer Moore J, Stroud R. Structure of tRNA pseudouridine synthase TruB and its RNA complex: RNA recognition through a combination of rigid docking and induced fit. Proc Natl Acad Sci U S A. 2003;100:12648-53 pubmed publisher
    RNA pseudouridine synthase, TruB, catalyzes pseudouridine formation at U55 in tRNA. This posttranscriptional modification is almost universally conserved and occurs in the T arm of most tRNAs...
  8. Nurse K, Wrzesinski J, Bakin A, Lane B, Ofengand J. Purification, cloning, and properties of the tRNA psi 55 synthase from Escherichia coli. RNA. 1995;1:102-12 pubmed
    ..86 min on the E. coli chromosome between the infB and rpsO genes. The proposed name for this gene is truB. There is very little protein sequence homology between the truB gene product and the hisT (truA) product, which ..
  9. Spedaliere C, Hamilton C, Mueller E. Functional importance of motif I of pseudouridine synthases: mutagenesis of aligned lysine and proline residues. Biochemistry. 2000;39:9459-65 pubmed
    ..of these alignments by substituting the nearly invariant lysine and proline residues in Motif I of RluA and TruB, pseudouridine synthases belonging to different families...

More Information


  1. Zucchini C, Strippoli P, Biolchi A, Solmi R, Lenzi L, D Addabbo P, et al. The human TruB family of pseudouridine synthase genes, including the Dyskeratosis Congenita 1 gene and the novel member TRUB1. Int J Mol Med. 2003;11:697-704 pubmed
    A novel human gene denominated TruB pseudouridine (psi) synthase homolog 1 (E. coli) (approved symbol, TRUB1) has been identified and characterized...
  2. Urbonavicius J, Durand J, Bj rk G. Three modifications in the D and T arms of tRNA influence translation in Escherichia coli and expression of virulence genes in Shigella flexneri. J Bacteriol. 2002;184:5348-57 pubmed
    ..The formation of these modified nucleosides is catalyzed by the products of genes trmH (Gm18), trmA (m(5)U54), and truB (Psi55)...