Gene Symbol: trkH
Description: potassium transporter
Alias: ECK3841, JW5576, sapJ
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Trchounian A, Kobayashi H. K+ uptake by fermenting Escherichia coli cells: pH dependent mode of the TrkA system operating. Biosci Rep. 2000;20:277-88 pubmed
    ..0 than that for cells grown at pH 7.3; (4) the DCCD-inhibited ATPase activity of membrane vesicles from bacteria grown at pH 9.0 was reduced a little in the presence of 100 mM K+, but stimulated more than 2.4-fold at pH 7.3. ..
  2. Stumpe S, Bakker E. Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch Microbiol. 1997;167:126-36 pubmed
    ..Cells that cannot take up K+ rapidly remain metabolically compromised to such an extent that extracytoplasmic protease activity is not induced, leading to a prolonged susceptibility of the cells to the toxic peptide. ..
  3. Schl sser A, Meldorf M, Stumpe S, Bakker E, Epstein W. TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli. J Bacteriol. 1995;177:1908-10 pubmed
    The corrected sequence of the trkH gene of Escherichia coli predicts that the TrkH protein is a hydrophobic membrane protein of 483 amino acid residues, of which 41% are identical to those of the homologous and functionally analogous TrkG ..
  4. Trchounian A, Vassilian A. Relationship between the F0F1-ATPase and the K(+)-transport system within the membrane of anaerobically grown Escherichia coli. N,N'-dicyclohexylcarbodiimide-sensitive ATPase activity in mutants with defects in K(+)-transport. J Bioenerg Biomembr. 1994;26:563-71 pubmed
    ..This effect was observed only in parent and in the trkG, but not in the trkA, trkE, or trkH mutants. The trkG or the trkH mutant with an unc deletion had a residual ATPase activity not sensitive to DCCD...
  5. Stumpe -, Bakker -. Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch Microbiol. 1997;167:126-36 pubmed
    ..Cells that cannot take up K+ rapidly remain metabolically compromised to such an extent that extracytoplasmic protease activity is not induced, leading to a prolonged susceptibility of the cells to the toxic peptide. ..
  6. Bossemeyer D, Borchard A, Dosch D, Helmer G, Epstein W, Booth I, et al. K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane. J Biol Chem. 1989;264:16403-10 pubmed
    ..Mutations in the three other genes implicated in Trk activity, trkE, trkG, and trkH, reduced or abolished the binding of TrkA to the membrane...
  7. Schlosser A, Hamann A, Bossemeyer D, Schneider E, Bakker E. NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport. Mol Microbiol. 1993;9:533-43 pubmed
    ..The nucleotide sequence of trkA, a gene encoding a surface component of the constitutive K(+)-uptake systems TrkG and TrkH from Escherichia coli, was determined...
  8. Parra Lopez C, Baer M, Groisman E. Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium. EMBO J. 1993;12:4053-62 pubmed
    ..The SapABCDF system constitutes a novel transporter for enteric bacteria and the first one harboring a periplasmic component with a role in virulence...
  9. Groisman E, Parra Lopez C, Salcedo M, Lipps C, Heffron F. Resistance to host antimicrobial peptides is necessary for Salmonella virulence. Proc Natl Acad Sci U S A. 1992;89:11939-43 pubmed
    ..Our data indicate that the ability to resist the killing effect of host antimicrobial peptides is a virulence property and that several resistance mechanisms operate in S. typhimurium. ..

More Information


  1. Guo Y, Xue Y, Liu J, Wang Q, Ma Y. Characterization and function analysis of a Halo-alkaline-adaptable Trk K+ uptake system in Alkalimonas amylolytica strain N10. Sci China C Life Sci. 2009;52:949-57 pubmed publisher
    ..These two genes, Aa-trkA (1337 bp) and Aa-trkH (1452 bp), were adjacent on the A. amylolytica N10 chromosome and transcribed in opposite directions...
  2. Trchunian A, Durgar ian S, Ogandzhanian E, Ter Nikogosian V, Vardanian A. [The capacity of anaerobically grown bacteria to exchange the 2H+ of the cell for the K+ of the medium and to maintain a high K+ distribution between cell and medium]. Nauchnye Doki Vyss Shkoly Biol Nauki. 1986;:82-8 pubmed
  3. Bagramyan K, Martirosov S. Formation of an ion transport supercomplex in Escherichia coli. An experimental model of direct transduction of energy. FEBS Lett. 1989;246:149-52 pubmed
    ..Within this supercomplex direct transduction of energy is accomplished via oxidation of 2 SH to S-S. ..
  4. Trchunian A, Ogandzhanian E, Mironova G, Martirosov S. [K+-dependent enzyme activity of H+-ATPase in the E. coli membrane]. Biofizika. 1991;36:102-4 pubmed
    ..coli depended on K+ activity. F1F0 include two additional proteins which correspond to the Trk system. The data improve the possibility to form supercomplex (F1F0-Trk) functioning as the H(+)-K(+)-pump. ..
  5. Buurman E, McLaggan D, Naprstek J, Epstein W. Multiple paths for nonphysiological transport of K+ in Escherichia coli. J Bacteriol. 2004;186:4238-45 pubmed
    ..In contrast, expression of wild-type ProP, TrkG, and TrkH proteins increased uptake when encoded on multicopy plasmids...
  6. Ukai H, Matsuzawa H, Ito K, Yamada M, Nishimura A. ftsE(Ts) affects translocation of K+-pump proteins into the cytoplasmic membrane of Escherichia coli. J Bacteriol. 1998;180:3663-70 pubmed
    ..We expressed alkaline phosphatase (PhoA) fusion proteins of KdpA, Kup, and TrkH, all of which proved functional in vivo as K+ ion pumps, in the mutant cells...
  7. Trchunian A, Ogandzhanian E, Martirosov S. [N,N'-dicyclohexylcarbodiimide-sensitive K+-dependent ATPase activity in Escherichia coli]. Nauchnye Doki Vyss Shkoly Biol Nauki. 1989;:90-5 pubmed
  8. Harms C, Domoto Y, Celik C, Rahe E, Stumpe S, Schmid R, et al. Identification of the ABC protein SapD as the subunit that confers ATP dependence to the K+-uptake systems Trk(H) and Trk(G) from Escherichia coli K-12. Microbiology. 2001;147:2991-3003 pubmed
    ..coli ATP dependence of Trk activity can be independent of Sap proteins. These data are interpreted to mean that Trk systems can interact with an ABC protein other than SapD...
  9. Nakahigashi K, Inokuchi H. Nucleotide sequence between the fadB gene and the rrnA operon from Escherichia coli. Nucleic Acids Res. 1990;18:6439 pubmed
  10. Trchounian A. Ion exchange in facultative anaerobes: does a proton-potassium pump exist in anaerobic Escherichia Coli?. Anaerobe. 1997;3:355-71 pubmed
  11. Epstein W, Buurman E, McLaggan D, Naprstek J. Multiple mechanisms, roles and controls of K+ transport in Escherichia coli. Biochem Soc Trans. 1993;21:1006-10 pubmed
  12. Trchunian A, Vasilian A. [ATPase activity and K+ transport in membranes of anaerobically grown trk-mutants of Escherichia coli]. Biokhimiia. 1993;58:1062-70 pubmed
    ..but not that of Na+, CL- or NO3-) and only in the wild precursor strain and trkG (but not in trkA, trkE, or trkH mutants)...
  13. Stewart L, Bakker E, Booth I. Energy coupling to K+ uptake via the Trk system in Escherichia coli: the role of ATP. J Gen Microbiol. 1985;131:77-85 pubmed
    ..Using inhibitors and unc mutants we show that Trk is not an ATPase but may be regulated by ATP. Possible mechanisms of energy coupling to Trk are discussed. ..
  14. Parra Lopez C, Lin R, Aspedon A, Groisman E. A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium. EMBO J. 1994;13:3964-72 pubmed
    ..Strains with mutations in sapG and either sapJ or the sapABCDF operon were as susceptible as sapG single mutants, suggesting that the proteins encoded by these ..
  15. Trchounian A, Bagramyan K, Vassilian A, Poladian A. Relationship between formate hydrogen lyase and proton-potassium pump under heterolactic fermentation in Escherichia coli: functional multienzyme associations in the cell membrane. Membr Cell Biol. 2000;13:511-26 pubmed
    ..The number of SH groups decreased in the presence of DCC. These results indicate a significance of the thiol groups for the function of the proposed association. ..
  16. Dosch D, Helmer G, Sutton S, Salvacion F, Epstein W. Genetic analysis of potassium transport loci in Escherichia coli: evidence for three constitutive systems mediating uptake potassium. J Bacteriol. 1991;173:687-96 pubmed
    ..of K+ for growth has revealed two new genes, trkG, near minute 30 within the cryptic rac prophage, and trkH, near minute 87, the products of which affect constitutive K+ transport...