Gene Symbol: tpx
Description: lipid hydroperoxide peroxidase
Alias: ECK1320, JW1317, yzzJ
Species: Escherichia coli str. K-12 substr. MG1655
Products:     tpx

Top Publications

  1. Dubbs J, Mongkolsuk S. Peroxiredoxins in bacterial antioxidant defense. Subcell Biochem. 2007;44:143-93 pubmed
    ..Studies examining the regulation and physiological roles of AhpC, Tpx, Ohr and OsmC reveal the multilayered nature of bacterial peroxide defense...
  2. Choi J, Choi S, Choi J, Cha M, Kim I, Shin W. Crystal structure of Escherichia coli thiol peroxidase in the oxidized state: insights into intramolecular disulfide formation and substrate binding in atypical 2-Cys peroxiredoxins. J Biol Chem. 2003;278:49478-86 pubmed
    Thioredoxin-dependent thiol peroxidase (Tpx) from Escherichia coli represents a group of antioxidant enzymes that are widely distributed in pathogenic bacterial species and which belong to the peroxiredoxin (Prx) family...
  3. Cha M, Kim H, Kim I. Mutation and Mutagenesis of thiol peroxidase of Escherichia coli and a new type of thiol peroxidase family. J Bacteriol. 1996;178:5610-4 pubmed
    ..In an attempt to perform physiological and biochemical characterizations of the thiol Px, a thiol Px null (tpx) mutant and a functional-residue mutant of thiol Px were produced...
  4. Baker L, Poole L. Catalytic mechanism of thiol peroxidase from Escherichia coli. Sulfenic acid formation and overoxidation of essential CYS61. J Biol Chem. 2003;278:9203-11 pubmed
    Escherichia coli thiol peroxidase (Tpx, p20, scavengase) is part of an oxidative stress defense system that uses reducing equivalents from thioredoxin (Trx1) and thioredoxin reductase to reduce alkyl hydroperoxides...
  5. Fourquet S, Huang M, D Autréaux B, Toledano M. The dual functions of thiol-based peroxidases in H2O2 scavenging and signaling. Antioxid Redox Signal. 2008;10:1565-76 pubmed publisher
    ..We then discuss possible thiol peroxidase physiologic functions, based on selected observations made in microorganisms and mammals. ..
  6. Tao K. Subcellular localization and in vivo oxidation-reduction kinetics of thiol peroxidase in Escherichia coli. FEMS Microbiol Lett. 2008;289:41-5 pubmed publisher
    ..Thiol peroxidase (Tpx) is one of the peroxiredoxins identified in Escherichia coli...
  7. Hofmann B, Hecht H, Flohe L. Peroxiredoxins. Biol Chem. 2002;383:347-64 pubmed
    ..In higher animals peroxiredoxins appear to be involved in the redox-regulation of cellular signaling and differentiation, displaying in part opposite effects. ..
  8. Choi J, Choi S, Choi J, Cha M, Kim I, Shin W. Crystallization and preliminary X-ray analysis of Escherichia coli p20, a novel thiol peroxidase. Acta Crystallogr D Biol Crystallogr. 2003;59:1064-6 pubmed
    ..97, b = 58.97, c = 127.59 A. The asymmetric unit contains two p20 molecules, with a corresponding V(M) of 2.06 A(3) Da(-1) and a solvent content of 40.4%. ..
  9. Hall A, Sankaran B, Poole L, Karplus P. Structural changes common to catalysis in the Tpx peroxiredoxin subfamily. J Mol Biol. 2009;393:867-81 pubmed publisher
    ..Here, we present three structures of Escherichia coli Tpx representing the fully folded (peroxide binding site intact), locally unfolded (disulfide bond), and partially ..

More Information


  1. Zhou Y, Wan X, Wang H, Yan Z, Hou Y, Jin D. Bacterial scavengase p20 is structurally and functionally related to peroxiredoxins. Biochem Biophys Res Commun. 1997;233:848-52 pubmed
    ..Taken together, our findings strongly suggest that the p20 scavengases are structurally and functionally related to peroxiredoxins. ..
  2. Beckham K, Byron O, Roe A, Gabrielsen M. The structure of an orthorhombic crystal form of a 'forced reduced' thiol peroxidase reveals lattice formation aided by the presence of the affinity tag. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:522-6 pubmed publisher
    Thiol peroxidase (Tpx) is an atypical 2-Cys peroxiredoxin, which has been suggested to be important for cell survival and virulence in Gram-negative pathogens...
  3. Cha M, Kim W, Lim C, Kim K, Kim I. Escherichia coli periplasmic thiol peroxidase acts as lipid hydroperoxide peroxidase and the principal antioxidative function during anaerobic growth. J Biol Chem. 2004;279:8769-78 pubmed
    ..Taken together, present data uncover in vivo function for p20 as a lipid hydroperoxide peroxidase and demonstrate that, as the result, p20 acts as the principal antioxidant in the anaerobic habitats. ..
  4. Kim S, Han Y, Kim I, Kim H. Involvement of ArcA and Fnr in expression of Escherichia coli thiol peroxidase gene. IUBMB Life. 1999;48:215-8 pubmed
    To explore the oxygen response regulators involved in thiol peroxidase gene (tpx) expression in Escherichia coli, we constructed a single-copy tpx-lacZ operon fusion and monitored tpx-lacZ expression in various genetic backgrounds...
  5. Baglioni P, Bini L, Liberatori S, Pallini V, Marri L. Proteome analysis of Escherichia coli W3110 expressing an heterologous sigma factor. Proteomics. 2003;3:1060-5 pubmed
    ..Among the proteins identified, thiol peroxidase (Tpx) was the only one up-regulated. The possible involvement of bgl and tpx in the survival of the pathogen E...
  6. Wood Z, Schröder E, Robin Harris J, Poole L. Structure, mechanism and regulation of peroxiredoxins. Trends Biochem Sci. 2003;28:32-40 pubmed
    ..Using crystal structures, a detailed catalytic cycle has been derived for typical 2-Cys Prxs, including a model for the redox-regulated oligomeric state proposed to control enzyme activity. ..
  7. Cha M, Kim H, Kim I. Thioredoxin-linked "thiol peroxidase" from periplasmic space of Escherichia coli. J Biol Chem. 1995;270:28635-41 pubmed
    ..This 20-kDa protein is thus named "thiol peroxidase," which could act as an antioxidant enzyme removing peroxides or H2O2 within the catalase- and peroxidase-deficient periplasmic space of E. coli...
  8. Kim H, Kim S, Lee J, Cha M, Kim I. Identification of promoter in the 5'-flanking region of the E. coli thioredoxin-linked thiol peroxidase gene: evidence for the existence of oxygen-related transcriptional regulatory protein. Biochem Biophys Res Commun. 1996;221:641-6 pubmed
    E. coli thiol peroxidase (Tpx) linked to the thioredoxin as an in vivo thiol regenerating system acts as an antioxidant enzyme removing peroxides and H2O2. In order to elucidate the mechanism regulating tpx gene expression in E...