Gene Symbol: tpiA
Description: triosephosphate isomerase
Alias: ECK3911, JW3890, tpi
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Hellinga H, Evans P. Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase. Eur J Biochem. 1985;149:363-73 pubmed
    ..coli. The region which has been sequenced comprises the complete pfkA--tpi interval on the E. coli genetic map...
  2. Mainfroid V, Goraj K, Rentier Delrue F, Houbrechts A, Loiseau A, Gohimont A, et al. Replacing the (beta alpha)-unit 8 of E.coli TIM with its chicken homologue leads to a stable and active hybrid enzyme. Protein Eng. 1993;6:893-900 pubmed
    ..Our kinetic studies show that the modification performed here leads to an active enzyme. The stability studies indicate that the stability of ETIM8CHI is comparable to that of the wild type TIM. ..
  3. Siddiquee K, Arauzo Bravo M, Shimizu K. Effect of a pyruvate kinase (pykF-gene) knockout mutation on the control of gene expression and metabolic fluxes in Escherichia coli. FEMS Microbiol Lett. 2004;235:25-33 pubmed
    ..RT-PCR revealed that the glycolytic genes such as glk, pgi, pfkA and tpiA were down regulated, that ppc, pckA, maeB and mdh genes were strongly up-regulated, and that the oxidative pentose ..
  4. Shimosaka M, Fukuda Y, Murata K, Kimura A. Application of hybrid plasmids carrying glycolysis genes to ATP production by Escherichia coli. J Bacteriol. 1982;152:98-103 pubmed
    The closely linked structural genes of phosphofructokinase (pfkA) and triosephosphate isomerase (tpi) of Escherichia coli were separately cloned onto plasmid pBR322. By gene dosage effects, transformed cells of E...
  5. Anderson A, Cooper R. Gluconeogenesis in Escherichia coli The role of triose phosphate isomerase. FEBS Lett. 1969;4:19-20 pubmed
  6. Fong S, Nanchen A, Palsson B, Sauer U. Latent pathway activation and increased pathway capacity enable Escherichia coli adaptation to loss of key metabolic enzymes. J Biol Chem. 2006;281:8024-33 pubmed
    ..evolution of four metabolic gene deletion mutants of Escherichia coli (delta pgi, delta ppc, delta pta, and delta tpi) in parallel evolution experiments of each mutant...
  7. Thomson J, Gerstenberger P, Goldberg D, Gociar E, Orozco de Silva A, Fraenkel D. ColE1 hybrid plasmids for Escherichia coli genes of glycolysis and the hexose monophosphate shunt. J Bacteriol. 1979;137:502-6 pubmed
    ..Plasmids were identified for phosphofructokinase (pfkA), triose phosphate isomerase (tpi), phosphoglucose isomerase (pgi), glucose-6-phosphate dehydrogenase (zwf), gluconate-6-phosphate dehydrogenase (gnd)..
  8. Bairoch A. PROSITE: a dictionary of sites and patterns in proteins. Nucleic Acids Res. 1992;20 Suppl:2013-8 pubmed
  9. Richard J. Restoring a metabolic pathway. ACS Chem Biol. 2008;3:605-7 pubmed publisher
    ..The origin of cellular L-GAP remains to be determined. ..

More Information


  1. Pichersky E, Gottlieb L, Hess J. Nucleotide sequence of the triose phosphate isomerase gene of Escherichia coli. Mol Gen Genet. 1984;195:314-20 pubmed
    ..To our knowledge, this is the first report of the sequence of a gene coding a glycolytic enzyme from a prokaryotic organism. ..
  2. Fong S, Palsson B. Metabolic gene-deletion strains of Escherichia coli evolve to computationally predicted growth phenotypes. Nat Genet. 2004;36:1056-8 pubmed
    ..These results show that computational models can be used to predict the eventual effects of genetic modifications. ..
  3. Otsuka Y, Koga M, Iwamoto A, Yonesaki T. A role of RnlA in the RNase LS activity from Escherichia coli. Genes Genet Syst. 2007;82:291-9 pubmed
    ..Among these, triose phosphate isomerase exhibited a remarkable affinity to RnlA. These results suggest that RnlA plays a central role in RNase LS activity and that its activity is regulated by multiple components...
  4. Hauser R, Ceol A, Rajagopala S, Mosca R, Siszler G, Wermke N, et al. A second-generation protein-protein interaction network of Helicobacter pylori. Mol Cell Proteomics. 2014;13:1318-29 pubmed publisher
    ..coli of which one third turned out to be conserved in both species. ..
  5. Kirkpatrick C, Maurer L, Oyelakin N, Yoncheva Y, Maurer R, Slonczewski J. Acetate and formate stress: opposite responses in the proteome of Escherichia coli. J Bacteriol. 2001;183:6466-77 pubmed
  6. Tiwari N, Woods L, Haley R, Kight A, Goforth R, Clark K, et al. Identification and characterization of native proteins of Escherichia coli BL-21 that display affinity towards Immobilized Metal Affinity Chromatography and Hydrophobic Interaction Chromatography Matrices. Protein Expr Purif. 2010;70:191-5 pubmed publisher
    ..coli strain(s) deficient in certain genomic proteins, and the design of an IMAC-HIC affinity tail for recombinant protein isolation based on the very proteins deleted from the genome. ..