tolC

Summary

Gene Symbol: tolC
Description: transport channel
Alias: ECK3026, JW5503, colE1-i, mtcB, mukA, refI, toc, weeA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Misra R, Reeves P. Role of micF in the tolC-mediated regulation of OmpF, a major outer membrane protein of Escherichia coli K-12. J Bacteriol. 1987;169:4722-30 pubmed
    Mutation in the tolC locus greatly reduces normal synthesis of OmpF, a major porin protein of Escherichia coli K-12...
  2. Morona R, Reeves P. The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins. J Bacteriol. 1982;150:1016-23 pubmed
    b>tolC mutants, which are resistant to colicin E1 and also highly sensitive to detergents and dyes, were shown to lack the OmpF outer membrane protein...
  3. Masi M, Vuong P, Humbard M, Malone K, Misra R. Initial steps of colicin E1 import across the outer membrane of Escherichia coli. J Bacteriol. 2007;189:2667-76 pubmed
    ..ColE1 initially binds to the vitamin B(12) receptor BtuB and then translocates through the TolC channel-tunnel, presumably in a mostly unfolded state. Here, we studied the early events in the import of ColE1...
  4. Fralick J. Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli. J Bacteriol. 1996;178:5803-5 pubmed
    A study examining the influence of TolC on AcrA, AcrR, and MarR1 mutants indicates that functional TolC is required for the operation of the AcrAB efflux system and for the expression of the Mar phenotype...
  5. Andersen C, Hughes C, Koronakis V. Electrophysiological behavior of the TolC channel-tunnel in planar lipid bilayers. J Membr Biol. 2002;185:83-92 pubmed
    Escherichia coli TolC assembles into the unique channel-tunnel structure spanning the outer membrane and periplasmic space...
  6. Koronakis V, Eswaran J, Hughes C. Structure and function of TolC: the bacterial exit duct for proteins and drugs. Annu Rev Biochem. 2004;73:467-89 pubmed
    The bacterial TolC protein plays a common role in the expulsion of diverse molecules, which include protein toxins and antibacterial drugs, from the cell...
  7. Werner J, Misra R. YaeT (Omp85) affects the assembly of lipid-dependent and lipid-independent outer membrane proteins of Escherichia coli. Mol Microbiol. 2005;57:1450-9 pubmed
    ..the two distinct groups of OMPs that follow either SurA- and lipopolysaccharide-dependent (OmpF/C) or -independent (TolC) folding pathways were affected, suggesting that YaeT may act as a general OMP assembly factor...
  8. Elkins C, Mullis L. Substrate competition studies using whole-cell accumulation assays with the major tripartite multidrug efflux pumps of Escherichia coli. Antimicrob Agents Chemother. 2007;51:923-9 pubmed
    AcrAB-TolC is the major, constitutively expressed tripartite multidrug efflux system in Escherichia coli that recognizes various structurally unrelated molecules, including many antibiotics, dyes, and steroids...
  9. Koronakis V, Li J, Koronakis E, Stauffer K. Structure of TolC, the outer membrane component of the bacterial type I efflux system, derived from two-dimensional crystals. Mol Microbiol. 1997;23:617-26 pubmed
    b>TolC is an outer membrane protein required for the export of virulence proteins and toxic compounds without a periplasmic intermediate...

More Information

Publications73

  1. Lobedanz S, Bokma E, Symmons M, Koronakis E, Hughes C, Koronakis V. A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps. Proc Natl Acad Sci U S A. 2007;104:4612-7 pubmed
    ..A key event in pump assembly is the recruitment of an outer membrane-anchored TolC exit duct by the adaptor protein of a cognate inner membrane translocase, establishing a contiguous transenvelope ..
  2. Hackett J, Misra R, Reeves P. The TolC protein of Escherichia coli K12 is synthesised in a precursor form. FEBS Lett. 1983;156:307-10 pubmed
    We examined the biosynthesis of the TolC protein of Escherichia coli K12 in a pulse-chase experiment, followed by immunoprecipitation with anti-TolC antibody and SDS-PAGE of the immunoprecipitate...
  3. Li H, Wang B, Xu W, Lin X, Peng X. Identification and network of outer membrane proteins regulating streptomysin resistance in Escherichia coli. J Proteome Res. 2008;7:4040-9 pubmed publisher
    ..b>TolC, OmpT and LamB were found to be up-regulated, and FadL, OmpW and a location-unknown protein Dps were down-regulated ..
  4. Wu L, Lin X, Peng X. From proteome to genome for functional characterization of pH-dependent outer membrane proteins in Escherichia coli. J Proteome Res. 2009;8:1059-70 pubmed publisher
    ..Among the differentially expressed proteins identified, OstA, TolC, OmpT, OmpP OmpC, Trak, OmpX, Dps, LamB, Tsx, FadL, OmpW, and OmpF were characterized as pH-related OM proteins...
  5. Stegmeier J, Polleichtner G, Brandes N, Hotz C, Andersen C. Importance of the adaptor (membrane fusion) protein hairpin domain for the functionality of multidrug efflux pumps. Biochemistry. 2006;45:10303-12 pubmed
    ..we have modeled the interaction of the hairpin domain and the channel tunnel on a molecular level for AcrA and TolC as well as MexA and OprM, respectively...
  6. Nagakubo S, Nishino K, Hirata T, Yamaguchi A. The putative response regulator BaeR stimulates multidrug resistance of Escherichia coli via a novel multidrug exporter system, MdtABC. J Bacteriol. 2002;184:4161-7 pubmed
    ..A BLAST search suggested that such a heteromultimer-type RND exporter constitutes a unique family among gram-negative organisms. ..
  7. Wandersman C, Delepelaire P. TolC, an Escherichia coli outer membrane protein required for hemolysin secretion. Proc Natl Acad Sci U S A. 1990;87:4776-80 pubmed
    ..B. (1986) Curr. Top. Microbiol. Immunol. 125, 159-181]. We show here that an E. coli outer membrane protein, the TolC protein, encoded by a gene not located in the hly cluster, is specifically required for hemolysin secretion...
  8. Fernandez Recio J, Walas F, Federici L, Venkatesh Pratap J, Bavro V, Miguel R, et al. A model of a transmembrane drug-efflux pump from Gram-negative bacteria. FEBS Lett. 2004;578:5-9 pubmed
    ..A representative pump from Escherichia coli is an assembly of the trimeric outer-membrane protein TolC, which is an allosteric channel, the trimeric inner-membrane proton-antiporter AcrB, and the periplasmic protein, ..
  9. Tatsumi R, Wachi M. TolC-dependent exclusion of porphyrins in Escherichia coli. J Bacteriol. 2008;190:6228-33 pubmed publisher
    We found that Escherichia coli tolC mutants showed increased sensitivity to 5-aminolevulinic acid (ALA), a precursor of porphyrins...
  10. Bleuel C, Grosse C, Taudte N, Scherer J, Wesenberg D, Krauss G, et al. TolC is involved in enterobactin efflux across the outer membrane of Escherichia coli. J Bacteriol. 2005;187:6701-7 pubmed
    ..and high-performance liquid chromatography analyses demonstrated that the outer membrane channel tunnel protein TolC but none of the respective seven resistance nodulation cell division (RND) proteins CusA, AcrB, AcrD, AcrF, MdtF (..
  11. Higgins M, Bokma E, Koronakis E, Hughes C, Koronakis V. Structure of the periplasmic component of a bacterial drug efflux pump. Proc Natl Acad Sci U S A. 2004;101:9994-9 pubmed publisher
    ..consist of an inner membrane transporter such as the AcrB proton antiporter, an outer membrane exit duct of the TolC family, and a periplasmic protein known as the adaptor...
  12. Rosner J, Martin R. An excretory function for the Escherichia coli outer membrane pore TolC: upregulation of marA and soxS transcription and Rob activity due to metabolites accumulated in tolC mutants. J Bacteriol. 2009;191:5283-92 pubmed publisher
    Efflux pumps function to rid bacteria of xenobiotics, including antibiotics, bile salts, and organic solvents. TolC, which forms an outer membrane channel, is an essential component of several efflux pumps in Escherichia coli...
  13. Andersen C. Channel-tunnels: outer membrane components of type I secretion systems and multidrug efflux pumps of Gram-negative bacteria. Rev Physiol Biochem Pharmacol. 2003;147:122-65 pubmed
    ..Prototypes of these export systems are the hemolysin secretion system and the AcrAB/TolC drug efflux pump of Escherichia coli, which both employ TolC as an outer membrane component...
  14. Zhang A, Rosner J, Martin R. Transcriptional activation by MarA, SoxS and Rob of two tolC promoters using one binding site: a complex promoter configuration for tolC in Escherichia coli. Mol Microbiol. 2008;69:1450-5 pubmed publisher
    The Escherichia coli tolC encodes a major outer membrane protein with multiple functions in export (e.g. diverse xenobiotics, haemolysin) and as an attachment site for phage and colicins...
  15. Lomovskaya O, Lewis K, Matin A. EmrR is a negative regulator of the Escherichia coli multidrug resistance pump EmrAB. J Bacteriol. 1995;177:2328-34 pubmed
    ..Some of the chemicals that induce the pump serve as its substrates, suggesting that their extrusion is the natural function of the pump. ..
  16. Yamanaka H, Tadokoro S, Miyano M, Takahashi E, Kobayashi H, Okamoto K. Studies on the region involved in the transport activity of Escherichia coli TolC by chimeric protein analysis. Microb Pathog. 2007;42:184-92 pubmed
    Gram-negative bacteria possess the outer membrane protein TolC which acts as an exit duct across the outer membrane. However, the region involved in the transport activity of TolC has remained unclear...
  17. Vediyappan G, Borisova T, Fralick J. Isolation and characterization of VceC gain-of-function mutants that can function with the AcrAB multiple-drug-resistant efflux pump of Escherichia coli. J Bacteriol. 2006;188:3757-62 pubmed
    ..b>TolC is the outer membrane component of the resistance-nodulation-division AcrAB-TolC efflux pump of Escherichia coli...
  18. Eguchi Y, Oshima T, Mori H, Aono R, Yamamoto K, Ishihama A, et al. Transcriptional regulation of drug efflux genes by EvgAS, a two-component system in Escherichia coli. Microbiology. 2003;149:2819-28 pubmed
    ..The observed MDR also depended on an outer-membrane channel, TolC. Microarray and S1 mapping assays indicated that, in the presence of this constitutive mutant EvgS, the level of ..
  19. Seeger M, Schiefner A, Eicher T, Verrey F, Diederichs K, Pos K. Structural asymmetry of AcrB trimer suggests a peristaltic pump mechanism. Science. 2006;313:1295-8 pubmed
    The AcrA/AcrB/TolC complex spans the inner and outer membranes of Escherichia coli and serves as its major drug-resistance pump...
  20. Koronakis V, Sharff A, Koronakis E, Luisi B, Hughes C. Crystal structure of the bacterial membrane protein TolC central to multidrug efflux and protein export. Nature. 2000;405:914-9 pubmed
    ..by the reversible interaction of substrate-specific inner-membrane proteins with an outer-membrane protein of the TolC family, thus bypassing the intervening periplasm. Here we report the 2...
  21. Andersen C, Koronakis E, Bokma E, Eswaran J, Humphreys D, Hughes C, et al. Transition to the open state of the TolC periplasmic tunnel entrance. Proc Natl Acad Sci U S A. 2002;99:11103-8 pubmed
    The TolC channel-tunnel spans the bacterial outer membrane and periplasm, providing a large exit duct for protein export and multidrug efflux when recruited by substrate-engaged inner membrane complexes...
  22. Dhamdhere G, Zgurskaya H. Metabolic shutdown in Escherichia coli cells lacking the outer membrane channel TolC. Mol Microbiol. 2010;77:743-54 pubmed publisher
    The outer membrane channel TolC is a key component of multidrug efflux and type I secretion transporters in Escherichia coli...
  23. Wiriyathanawudhiwong N, Ohtsu I, Li Z, Mori H, Takagi H. The outer membrane TolC is involved in cysteine tolerance and overproduction in Escherichia coli. Appl Microbiol Biotechnol. 2009;81:903-13 pubmed publisher
    ..coli K-12 mutants (the Keio collection). Among the 3,985 nonessential gene mutants, tolC-disrupted cells showed hypersensitivity to L-cysteine relative to wild-type cells...
  24. Tamura N, Murakami S, Oyama Y, Ishiguro M, Yamaguchi A. Direct interaction of multidrug efflux transporter AcrB and outer membrane channel TolC detected via site-directed disulfide cross-linking. Biochemistry. 2005;44:11115-21 pubmed
    The AcrAB-TolC system exports a wide variety of drugs and toxic compounds, and confers intrinsic drug tolerance on Escherichia coli. The crystal structures suggested that AcrB and TolC directly dock with each other...
  25. Augustus A, Celaya T, Husain F, Humbard M, Misra R. Antibiotic-sensitive TolC mutants and their suppressors. J Bacteriol. 2004;186:1851-60 pubmed
    The TolC protein of Escherichia coli, through its interaction with AcrA and AcrB, is thought to form a continuous protein channel that expels inhibitors from the cell. Consequently, tolC null mutations display a hypersensitive phenotype...
  26. Touze T, Eswaran J, Bokma E, Koronakis E, Hughes C, Koronakis V. Interactions underlying assembly of the Escherichia coli AcrAB-TolC multidrug efflux system. Mol Microbiol. 2004;53:697-706 pubmed
    The major Escherichia coli multidrug efflux pump AcrAB-TolC expels a wide range of antibacterial agents...
  27. Yamanaka H, Morisada N, Miyano M, Tsuge H, Shinoda S, Takahashi E, et al. Amino-acid residues involved in the expression of the activity of Escherichia coli TolC. Microbiol Immunol. 2004;48:713-22 pubmed
    The Escherichia coli TolC, composed of 471 amino-acid residues, functions as a channel tunnel in the transport of various molecules across the outer membrane...
  28. Werner J, Augustus A, Misra R. Assembly of TolC, a structurally unique and multifunctional outer membrane protein of Escherichia coli K-12. J Bacteriol. 2003;185:6540-7 pubmed
    b>TolC is a multifunctional outer membrane protein of Escherichia coli that folds into a novel alpha-beta-barrel conformation absent in the other model outer membrane proteins used in assembly studies...
  29. Okusu H, Ma D, Nikaido H. AcrAB efflux pump plays a major role in the antibiotic resistance phenotype of Escherichia coli multiple-antibiotic-resistance (Mar) mutants. J Bacteriol. 1996;178:306-8 pubmed
    ..This experiment identified the AcrAB system as the major pump responsible for making the Mar mutants resistant to many agents, including tetracycline, chloramphenicol, ampicillin, nalidixic acid, and rifampin. ..
  30. Murakami S, Nakashima R, Yamashita E, Matsumoto T, Yamaguchi A. Crystal structures of a multidrug transporter reveal a functionally rotating mechanism. Nature. 2006;443:173-9 pubmed
    AcrB is a principal multidrug efflux transporter in Escherichia coli that cooperates with an outer-membrane channel, TolC, and a membrane-fusion protein, AcrA. Here we describe crystal structures of AcrB with and without substrates...
  31. Tikhonova E, Dastidar V, Rybenkov V, Zgurskaya H. Kinetic control of TolC recruitment by multidrug efflux complexes. Proc Natl Acad Sci U S A. 2009;106:16416-21 pubmed publisher
    ..to the membrane fusion protein (MFP) family; and outer membrane channels exemplified by the Escherichia coli TolC. The three-component complexes span the entire two-membrane envelope of Gram-negative bacteria and expel toxic ..
  32. Zgurskaya H, Nikaido H. Cross-linked complex between oligomeric periplasmic lipoprotein AcrA and the inner-membrane-associated multidrug efflux pump AcrB from Escherichia coli. J Bacteriol. 2000;182:4264-7 pubmed
    ..multiple antimicrobial agents are produced through expression of the three-component multidrug efflux system AcrAB-TolC. AcrB is a proton-motive-force-dependent transporter located in the inner membrane, and AcrA and TolC are accessory ..
  33. Vakharia H, German G, Misra R. Isolation and characterization of Escherichia coli tolC mutants defective in secreting enzymatically active alpha-hemolysin. J Bacteriol. 2001;183:6908-16 pubmed
    This study describes the isolation and characterization of a unique class of TolC mutants that, under steady-state growth conditions, secreted normal levels of largely inactive alpha-hemolysin...
  34. Tal N, Schuldiner S. A coordinated network of transporters with overlapping specificities provides a robust survival strategy. Proc Natl Acad Sci U S A. 2009;106:9051-6 pubmed publisher
    ..in an organism such as Escherichia coli mutations in genes coding for transporters other than the major AcrAB-TolC multidrug efflux transporter have only a marginal effect on phenotype...
  35. Bokma E, Koronakis E, Lobedanz S, Hughes C, Koronakis V. Directed evolution of a bacterial efflux pump: adaptation of the E. coli TolC exit duct to the Pseudomonas MexAB translocase. FEBS Lett. 2006;580:5339-43 pubmed
    Bacterial multidrug efflux pumps operate by periplasmic recruitment and opening of TolC family outer membrane exit ducts by cognate inner membrane translocases...
  36. Aono R, Tsukagoshi N, Yamamoto M. Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12. J Bacteriol. 1998;180:938-44 pubmed
    Escherichia coli mutants with improved organic solvent tolerance levels showed high levels of outer membrane protein TolC and inner membrane protein AcrA. The TolC level was regulated positively by MarA, Rob, or SoxS...
  37. Kobayashi N, Nishino K, Yamaguchi A. Novel macrolide-specific ABC-type efflux transporter in Escherichia coli. J Bacteriol. 2001;183:5639-44 pubmed
    ..In addition, MacAB required TolC for its function in a way similar to that of most of the MFP-dependent transporters in E. coli...
  38. Hackett J, Reeves P. Primary structure of the tolC gene that codes for an outer membrane protein of Escherichia coli K12. Nucleic Acids Res. 1983;11:6487-95 pubmed
    We present the nucleotide sequence of the tolC gene of Escherichia coli K12, and the amino acid sequence of the TolC protein (an outer membrane protein) as deduced from it...
  39. Yamanaka H, Izawa H, Okamoto K. Carboxy-terminal region involved in activity of Escherichia coli TolC. J Bacteriol. 2001;183:6961-4 pubmed
    The Escherichia coli TolC acts as a channel tunnel in the transport of various molecules across the outer membrane...
  40. Gerken H, Misra R. Genetic evidence for functional interactions between TolC and AcrA proteins of a major antibiotic efflux pump of Escherichia coli. Mol Microbiol. 2004;54:620-31 pubmed
    Genetic data have suggested that TolC, AcrA and AcrB constitute a major antibiotic efflux system in Escherichia coli...
  41. Bavro V, Pietras Z, Furnham N, Perez Cano L, Fernandez Recio J, Pei X, et al. Assembly and channel opening in a bacterial drug efflux machine. Mol Cell. 2008;30:114-21 pubmed publisher
    ..We describe two crystal structures of the Escherichia coli outer membrane protein TolC in its partially open state...
  42. Davies J, Reeves P. Genetics of resistance to colicins in Escherichia coli K-12: cross-resistance among colicins of group A. J Bacteriol. 1975;123:102-17 pubmed
    ..We have confirmed the position of previously isolated mutations on the Escherichia coli K-12 genetic map, and located approximately the loci conferring colicin resistance in some of the newly isolated mutants. ..
  43. Symmons M, Bokma E, Koronakis E, Hughes C, Koronakis V. The assembled structure of a complete tripartite bacterial multidrug efflux pump. Proc Natl Acad Sci U S A. 2009;106:7173-8 pubmed publisher
    ..a periplasmic adaptor protein connects a substrate-binding inner membrane transporter to an outer membrane-anchored TolC-type exit duct...
  44. Elkins C, Nikaido H. Chimeric analysis of AcrA function reveals the importance of its C-terminal domain in its interaction with the AcrB multidrug efflux pump. J Bacteriol. 2003;185:5349-56 pubmed
    AcrAB-TolC is the major, constitutively expressed efflux protein complex that provides resistance to a variety of antimicrobial agents in Escherichia coli...
  45. Moore S, Sauer R. Revisiting the mechanism of macrolide-antibiotic resistance mediated by ribosomal protein L22. Proc Natl Acad Sci U S A. 2008;105:18261-6 pubmed publisher
    ..mutation reduces antibiotic occupancy of the target site on ribosomes in a manner largely dependent on the AcrAB-TolC efflux system...
  46. Gotoh N, Murata T, Ozaki T, Kimura T, Kondo A, Nishino T. Intrinsic resistance of Escherichia coli to mureidomycin A and C due to expression of the multidrug efflux system AcrAB-TolC: comparison with the efflux systems of mureidomycin-susceptible Pseudomonas aeruginosa. J Infect Chemother. 2003;9:101-3 pubmed
    ..These studies showed that the differences regarding the susceptibility of E. coli and P. aeruginosa to mureidomycin A and C may be explained by the expression of efflux systems that mediate resistance to mureidomycin A and C. ..
  47. Wang C, Yang L, Shah A, Choi E, Kim S. Dynamic interplay of multidrug transporters with TolC for isoprenol tolerance in Escherichia coli. Sci Rep. 2015;5:16505 pubmed publisher
    ..AcrAB is the major transporter forming tripartite transperiplasmic complex with outer membrane channel TolC for direct extrusion of toxic molecules in E. coli...
  48. Zhang D, Jiang B, Xiang Z, Wang S. Functional characterisation of altered outer membrane proteins for tetracycline resistance in Escherichia coli. Int J Antimicrob Agents. 2008;32:315-9 pubmed publisher
    ..In this study, upregulation of FimD, Tsx, OmpW, OmpC and TolC and downregulation of LamB in response to tetracycline were detected and confirmed by two-dimensional gel ..
  49. Thanabalu T, Koronakis E, Hughes C, Koronakis V. Substrate-induced assembly of a contiguous channel for protein export from E.coli: reversible bridging of an inner-membrane translocase to an outer membrane exit pore. EMBO J. 1998;17:6487-96 pubmed
    ..IM) translocase of two proteins, HlyD and the traffic ATPase HlyB, and the outer-membrane (OM) porin-like TolC. These and the toxin substrate were expressed separately to reconstitute export and, via affinity tags on the IM ..
  50. Tikhonova E, Zgurskaya H. AcrA, AcrB, and TolC of Escherichia coli Form a Stable Intermembrane Multidrug Efflux Complex. J Biol Chem. 2004;279:32116-24 pubmed
    ..interactions between the inner and outer membrane components of the tri-partite multidrug efflux pump AcrAB-TolC from Escherichia coli...
  51. Takahashi E, Okamoto K, Arimoto S, Yamanaka H, Negishi T. Involvement of the drug efflux protein TolC in mutagenicity induced by MNNG or Trp-P-2. Mutat Res. 2006;605:42-50 pubmed
    ..In this study, we constructed a series of Escherichia coli CC strains lacking the TolC protein to determine if mutation is actually enhanced by the inhibition of mutagen reflux...
  52. Elkins C, Beenken K. Modeling the tripartite drug efflux pump archetype: structural and functional studies of the macromolecular constituents reveal more than their names imply. J Chemother. 2005;17:581-92 pubmed
    ..However, correct assembly of the components is still a matter of debate as is the functional contribution of each to the translocation of drug substrates over long distances spanning the Gram-negative cell envelope. ..
  53. Kincses A, Szabó A, Saijo R, Watanabe G, Kawase M, Molnar J, et al. Fluorinated Beta-diketo Phosphorus Ylides Are Novel Efflux Pump Inhibitors in Bacteria. In Vivo. 2016;30:813-817 pubmed
    ..subunits acridine-resistance proteins A and B (AcrA and AcrB) and the multidrug efflux pump outer membrane factor TolC (TolC) of Escherichia coli K-12 AG100 strain and its AcrAB-TolC-deleted strain...
  54. Delgado M, Solbiati J, Chiuchiolo M, Farias R, Salomon R. Escherichia coli outer membrane protein TolC is involved in production of the peptide antibiotic microcin J25. J Bacteriol. 1999;181:1968-70 pubmed
    A Tn5 insertion in tolC eliminated microcin J25 production. The mutation had little effect on the expression of the microcin structural gene and presumably acted by blocking microcin secretion...
  55. Liu J, Pan Y, Yuan L, Wu H, Hu G, Chen Y. Genetic variations in the active efflux pump genes acrA/B and tolC in different drug-induced strains of Escherichia coli CVCC 1547. Genet Mol Res. 2013;12:2829-36 pubmed publisher
    This study aimed to investigate the properties of mutations of the active efflux pump genes acrA/B and tolC in Escherichia coli CVCC 1547 when induced by different drugs. The mutations were isolated in vitro by exposing E...
  56. Nishino K, Senda Y, Yamaguchi A. CRP regulator modulates multidrug resistance of Escherichia coli by repressing the mdtEF multidrug efflux genes. J Antibiot (Tokyo). 2008;61:120-7 pubmed publisher
    ..caused by crp deletion was completely suppressed by deletion of the multifunctional outer membrane channel gene tolC. TolC interacts with different drug efflux pumps. Among the twenty drug efflux pumps in E...
  57. Muela A, Seco C, Camafeita E, Arana I, Orruño M, Lopez J, et al. Changes in Escherichia coli outer membrane subproteome under environmental conditions inducing the viable but nonculturable state. FEMS Microbiol Ecol. 2008;64:28-36 pubmed publisher
    ..However, proteins exclusive to the VBNC state were not detected. ..
  58. Schmidt T, Raunest M, Fischer N, Reith D, Kandt C. Computer simulations suggest direct and stable tip to tip interaction between the outer membrane channel TolC and the isolated docking domain of the multidrug RND efflux transporter AcrB. Biochim Biophys Acta. 2016;1858:1419-26 pubmed publisher
    ..of multi-drug efflux pumps of the resistance nodulation division (RND) protein super family of which AcrAB-TolC in Escherichia coli is a prominent example...
  59. Raji A, Zabel D, Laufer C, Depew R. Genetic analysis of mutations that compensate for loss of Escherichia coli DNA topoisomerase I. J Bacteriol. 1985;162:1173-9 pubmed
    ..loci: gyrA and gyrB, the genes that encode the subunits of DNA gyrase, and a previously unidentified locus near tolC. Mutations at this third locus, referred to as toc (topoisomerase one compensatory) mutations, do not behave as ..
  60. Corbalán N, Adler C, de Cristóbal R, Pomares M, Delgado M, Vincent P. The tolC locus affects the expression of sbmA through ?E activity increase. FEMS Microbiol Lett. 2010;311:185-92 pubmed publisher
    ..Previously, we found that a combination of sbmA and tolC mutations in Tn10-carrying E. coli K-12 strains results in hypersusceptibility to tetracycline...
  61. Wang Z, Fan G, Hryc C, Blaza J, Serysheva I, Schmid M, et al. An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump. elife. 2017;6: pubmed publisher
    ..Here, we report the near-atomic resolution cryoEM structures of the Escherichia coli AcrAB-TolC multidrug efflux pump in resting and drug transport states, revealing a quaternary structural switch that ..
  62. Ge Q, Yamada Y, Zgurskaya H. The C-terminal domain of AcrA is essential for the assembly and function of the multidrug efflux pump AcrAB-TolC. J Bacteriol. 2009;191:4365-71 pubmed publisher
    ..role of the C-terminal domain of Escherichia coli AcrA, a periplasmic component of the multidrug efflux pump AcrAB-TolC. Using trypsin proteolysis, we identified the proteolytically labile sites in the C-terminal domain (amino acid ..
  63. Hirakawa H, Inazumi Y, Masaki T, Hirata T, Yamaguchi A. Indole induces the expression of multidrug exporter genes in Escherichia coli. Mol Microbiol. 2005;55:1113-26 pubmed
    ..BaeR and CpxR directly bound to different sequences of the acrD and mdtA promoter regions. These observations indicate that BaeR is a primary regulator, and CpxR enhances the effect of BaeR. ..
  64. Morona R, Manning P, Reeves P. Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli. J Bacteriol. 1983;153:693-9 pubmed
    We used the cloned tolC gene to identify, locate, and purify its gene product. Strains carrying pPR13 or pPR42 overproduced a cell envelope protein (molecular weight, 52,000)...