Gene Symbol: tmk
Description: thymidylate kinase
Alias: ECK1084, JW1084, ycfG
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Dong Z, Onrust R, Skangalis M, O Donnell M. DNA polymerase III accessory proteins. I. holA and holB encoding delta and delta'. J Biol Chem. 1993;268:11758-65 pubmed
    ..The predicted amino acid sequence of delta' is homologous to the sequences of the tau and gamma subunits revealing a large amount of structural redundancy within the holoenzyme. ..
  2. Carter J, Franden M, Aebersold R, McHenry C. Identification, isolation, and characterization of the structural gene encoding the delta' subunit of Escherichia coli DNA polymerase III holoenzyme. J Bacteriol. 1993;175:3812-22 pubmed
    ..The gene, when placed into a tac promoter-based expression plasmid, directed expression of two proteins of similar size. By immunodetection with anti-holoenzyme immunoglobulin G, both proteins are judged to be products of holB. ..
  3. Chenal Francisque V, Tourneux L, Carniel E, Christova P, Li de la Sierra I, B rzu O, et al. The highly similar TMP kinases of Yersinia pestis and Escherichia coli differ markedly in their AZTMP phosphorylating activity. Eur J Biochem. 1999;265:112-9 pubmed
    ..To characterize the TMP kinase of Yersinia pestis, a chromosomal region encompassing its gene (tmk) was cloned and sequenced; a high degree of conservation with the corresponding region of Escherichia coli was ..
  4. Chaperon D. Construction and complementation of in-frame deletions of the essential Escherichia coli thymidylate kinase gene. Appl Environ Microbiol. 2006;72:1288-94 pubmed
    This work reports the construction of Escherichia coli in-frame deletion strains of tmk, which encodes thymidylate kinase, Tmk. The tmk gene is located at the third position of a putative five-gene operon at 24.9 min on the E...
  5. Bock A, Forchhammer K, Heider J, Baron C. Selenoprotein synthesis: an expansion of the genetic code. Trends Biochem Sci. 1991;16:463-7 pubmed
    ..In E. coli, this requires a specific translation factor. Selenocysteine thus represents a unique expansion of the genetic code. ..
  6. Binkley J, Kuempel P. Genetic mapping in Escherichia coli of tmk, the locus for dTMP kinase. J Bacteriol. 1986;168:1457-8 pubmed
    The genetic location of tmk, the gene for dTMP kinase, has been mapped at min 24.0 on the Escherichia coli map.
  7. Nelson D, Carter C. Purification and characterization of Thymidine 5-monophosphate kinase from Escherichia coli B. J Biol Chem. 1969;244:5254-62 pubmed
  8. Daws T, Fuchs J. Isolation and characterization of an Escherichia coli mutant deficient in dTMP kinase activity. J Bacteriol. 1984;157:440-4 pubmed
    ..All three of these phenotypes are cotransducible. The tmk gene was mapped by cotransduction to approximately 30 min on the E. coli map.
  9. Reynes J, Tiraby M, Baron M, Drocourt D, Tiraby G. Escherichia coli thymidylate kinase: molecular cloning, nucleotide sequence, and genetic organization of the corresponding tmk locus. J Bacteriol. 1996;178:2804-12 pubmed
    ..The nucleotide sequence of the tmk gene encoding this essential Escherichia coli enzyme is the last one among all the E...

More Information


  1. Echtenkamp P, Wilson D, Shuler M. Cell cycle progression in Escherichia coli B/r affects transcription of certain genes: Implications for synthetic genome design. Biotechnol Bioeng. 2009;102:902-9 pubmed publisher
    ..In conclusion, gene position, with regard to the C period, and gene function are important factors to incorporate into design criteria for synthetic bacterial genomes. ..