Gene Symbol: tisB
Description: toxic membrane persister formation peptide, LexA-regulated
Alias: ECK4427, ysdA, ysdB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Fernandez de Henestrosa A, Ogi T, Aoyagi S, Chafin D, Hayes J, Ohmori H, et al. Identification of additional genes belonging to the LexA regulon in Escherichia coli. Mol Microbiol. 2000;35:1560-72 pubmed
    ..These experiments have allowed us to identify seven new LexA-regulated genes, thus bringing the present number of genes in the E. coli LexA regulon to 31. The potential function of each newly identified LexA-regulated gene is discussed. ..
  2. Vogel J, Argaman L, Wagner E, Altuvia S. The small RNA IstR inhibits synthesis of an SOS-induced toxic peptide. Curr Biol. 2004;14:2271-6 pubmed
    ..This locus encodes two sRNAs, IstR-1 and IstR-2, and a toxic peptide, TisB, encoded by tisAB mRNA...
  3. Unoson C, Wagner E. A small SOS-induced toxin is targeted against the inner membrane in Escherichia coli. Mol Microbiol. 2008;70:258-70 pubmed publisher
    We previously reported on an SOS-induced toxin, TisB, in Escherichia coli and its regulation by the RNA antitoxin IstR-1. Here, we addressed the mode of action of TisB...
  4. Darfeuille F, Unoson C, Vogel J, Wagner E. An antisense RNA inhibits translation by competing with standby ribosomes. Mol Cell. 2007;26:381-92 pubmed
    ..Standby binding is required for initiation at the highly structured tisB TIR. This may involve ribosome sliding to a transiently open tisB TIR...
  5. Fozo E, Kawano M, Fontaine F, Kaya Y, Mendieta K, Jones K, et al. Repression of small toxic protein synthesis by the Sib and OhsC small RNAs. Mol Microbiol. 2008;70:1076-93 pubmed publisher
    ..Whole genome expression analysis showed that overexpression of IbsC and ShoB, as well as the small hydrophobic LdrD and TisB proteins, has both overlapping and unique consequences for the cell.
  6. Weel Sneve R, Bjørås M, Kristiansen K. Overexpression of the LexA-regulated tisAB RNA in E. coli inhibits SOS functions; implications for regulation of the SOS response. Nucleic Acids Res. 2008;36:6249-59 pubmed publisher
    ..These data shed new light on the complexity of SOS regulation in which the uxaA gene could link sugar metabolism to the SOS response via antisense regulation of the tisAB gene. ..
  7. Dörr T, Vulic M, Lewis K. Ciprofloxacin causes persister formation by inducing the TisB toxin in Escherichia coli. PLoS Biol. 2010;8:e1000317 pubmed publisher
    ..administration of ciprofloxacin induced persister formation in a tisAB-dependent manner, and cells producing TisB toxin were tolerant to multiple antibiotics...
  8. Gurnev P, Ortenberg R, Dörr T, Lewis K, Bezrukov S. Persister-promoting bacterial toxin TisB produces anion-selective pores in planar lipid bilayers. FEBS Lett. 2012;586:2529-34 pubmed publisher
    We studied membrane activity of the bacterial peptide TisB involved in persister cell formation. TisB and its analogs form multi-state ion-conductive pores in planar lipid bilayers with all states displaying similar anionic selectivity...
  9. Gerdes K, Wagner E. RNA antitoxins. Curr Opin Microbiol. 2007;10:117-24 pubmed
    ..Thus, it is possible that an ancestral antitoxin gene evolved into the present toxin gene (symE) whose translation is repressed by an RNA antitoxin (SymR). ..

More Information


  1. Berghoff B, Hoekzema M, Aulbach L, Wagner E. Two regulatory RNA elements affect TisB-dependent depolarization and persister formation. Mol Microbiol. 2017;103:1020-1033 pubmed publisher
    ..We investigated the TisB/IstR-1 toxin-antitoxin system of Escherichia coli to appreciate the role of the RNA antitoxin IstR-1 in TisB-..