tgt

Summary

Gene Symbol: tgt
Description: queuine tRNA-ribosyltransferase
Species: Escherichia coli O157:H7 str. Sakai

Top Publications

  1. Iwata Reuyl D. Biosynthesis of the 7-deazaguanosine hypermodified nucleosides of transfer RNA. Bioorg Chem. 2003;31:24-43 pubmed
    ..emphasis on the only two enzymes that have been discovered in the pathways: tRNA-guanine transglycosylase (TGT), which catalyzes the insertion of a modified base into the polynucleotide with the concomitant elimination of the ..
  2. Curnow A, Garcia G. tRNA-guanine transglycosylase from Escherichia coli. Minimal tRNA structure and sequence requirements for recognition. J Biol Chem. 1995;270:17264-7 pubmed
    Previously, we have demonstrated that the tRNA-guanine transglycosylase (TGT) from Escherichia coli is capable of utilizing an in vitro generated minihelix consisting of the anticodon stem and loop sequence of E...
  3. Nonekowski S, Kung F, Garcia G. The Escherichia coli tRNA-guanine transglycosylase can recognize and modify DNA. J Biol Chem. 2002;277:7178-82 pubmed
    tRNA-guanine transglycosylase (TGT) catalyzes the exchange of queuine (or a precursor) for guanine 34 in tRNA...
  4. Gardel C, Johnson K, Jacq A, Beckwith J. The secD locus of E.coli codes for two membrane proteins required for protein export. EMBO J. 1990;9:3209-16 pubmed
    ..We suggest that these two proteins may form a complex in the membrane which acts at late steps in the export process. ..
  5. Nonekowski S, Garcia G. tRNA recognition by tRNA-guanine transglycosylase from Escherichia coli: the role of U33 in U-G-U sequence recognition. RNA. 2001;7:1432-41 pubmed
    ..One of the key enzymes in this pathway, tRNA-guanine transglycosylase (TGT), exchanges the genetically encoded guanine at position 34 with a queuine precursor, preQ1...
  6. Dineshkumar T, Thanedar S, Subbulakshmi C, Varshney U. An unexpected absence of queuosine modification in the tRNAs of an Escherichia coli B strain. Microbiology. 2002;148:3779-87 pubmed
    ..However, the lack of queuosine in tRNAs was not a common feature of all E. coli B strains. The tgt and queA genes in B105 were shown to be functional by their ability to complement tgt and queA mutant strains...
  7. Kung F, Nonekowski S, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: recognition of noncognate-cognate chimeric tRNA and discovery of a novel recognition site within the TpsiC arm of tRNA(Phe). RNA. 2000;6:233-44 pubmed
    tRNA-guanine transglycosylase (TGT) is a key enzyme involved in the posttranscriptional modification of tRNA across the three kingdoms of life...
  8. Jänel G, Michelsen U, Nishimura S, Kersten H. Queuosine modification in tRNA and expression of the nitrate reductase in Escherichia coli. EMBO J. 1984;3:1603-8 pubmed
    ..Isogenic pairs of Escherichia coli, containing or lacking the tRNA-transglycosylase (JE 7335, tgt+ lacZ+ and JE 7337, tgt- lacZ+; JE 7334, tgt+ lacZ- and JE 7336, tgt- lacZ-), have been employed to study the ..
  9. Garcia G, Koch K, Chong S. tRNA-guanine transglycosylase from Escherichia coli. Overexpression, purification and quaternary structure. J Mol Biol. 1993;231:489-97 pubmed
    tRNA-guanine transglycosylase (TGT) is the enzyme responsible for the posttranscriptional modification of specific tRNAs (Asn, Asp, His and Tyr) with queuine. In E...

More Information

Publications32

  1. Chong S, Curnow A, Huston T, Garcia G. tRNA-guanine transglycosylase from Escherichia coli is a zinc metalloprotein. Site-directed mutagenesis studies to identify the zinc ligands. Biochemistry. 1995;34:3694-701 pubmed
    tRNA-guanine transglycosylase (TGT) from Escherichia coli catalyzes the exchange of the queuine precursor, preQ1, into tRNA as part of the biosynthetic pathway for the posttranscriptionally modified base, queuine...
  2. Pogliano K, Beckwith J. Genetic and molecular characterization of the Escherichia coli secD operon and its products. J Bacteriol. 1994;176:804-14 pubmed
    ..operon, and found that, in addition to secD and secF, it contains the upstream gene yajC, but not the genes queA or tgt, in contrast to previous reports...
  3. Garcia G, Chong S. Cysteine 265 is in the active site of, but is not essential for catalysis by tRNA-guanine transglycosylase (TGT) from Escherichia coli. J Protein Chem. 1997;16:11-7 pubmed
    ..mutagenesis and X-ray absorption spectroscopy studies have previously shown that the tRNA-guanine transglycosylase (TGT) from Escherichia coli is a zinc metalloprotein and identified the enzymic ligands to the zinc [Chong et al...
  4. Reuter K, Slany R, Ullrich F, Kersten H. Structure and organization of Escherichia coli genes involved in biosynthesis of the deazaguanine derivative queuine, a nutrient factor for eukaryotes. J Bacteriol. 1991;173:2256-64 pubmed
    The plasmid pPR20 contains the gene tgt, which encodes tRNA guanine transglycosylase (Tgt), on a 33-kbp DNA insert from a region around 9 min on the Escherichia coli linkage map...
  5. Curnow A, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: recognition of dimeric, unmodified tRNA(Tyr). Biochimie. 1994;76:1183-91 pubmed
    In order to probe the interaction between tRNA and the tRNA hypermodifying enzyme, tRNA-guanine transglycosylase (TGT) from Escherichia coli, we have undertaken the generation of E coli tRNA(Tyr) and analogues...
  6. Frey B, Jänel G, Michelsen U, Kersten H. Mutations in the Escherichia coli fnr and tgt genes: control of molybdate reductase activity and the cytochrome d complex by fnr. J Bacteriol. 1989;171:1524-30 pubmed
    In eubacteria, the tRNA transglycosylase (Tgt) in specific tRNAs exchanges a guanine in the anticodon for 7-aminomethyl-7-deazaguanine, which is finally converted to queuosine...
  7. Chong S, Garcia G. A versatile and general prokaryotic expression vector, pLACT7. Biotechniques. 1994;17:686, 688, 690-1 pubmed
    We have previously reported the constitutive over-expression of the tRNA-guanine transglycosylase (TGT) from plasmid pTGT1 in Escherichia coli...
  8. Gardel C, Johnson K, Jacq A, Beckwith J. The secD locus of E. coli codes for two membrane proteins required for protein export. EMBO J. 1990;9:4205-6 pubmed
  9. Kittendorf J, Barcomb L, Nonekowski S, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: molecular mechanism and role of aspartate 89. Biochemistry. 2001;40:14123-33 pubmed
    The enzyme tRNA-guanine transglycosylase (TGT, EC 2.4.2...
  10. Urbonavicius J, Stahl G, Durand J, Ben Salem S, Qian Q, Farabaugh P, et al. Transfer RNA modifications that alter +1 frameshifting in general fail to affect -1 frameshifting. RNA. 2003;9:760-8 pubmed
    Using mutants (tgt, mnmA(asuE, trmU), mnmE(trmE), miaA, miaB, miaE, truA(hisT), truB) of either Escherichia coli or Salmonella enterica serovar Typhimurium and the trm5 mutant of Saccharomyces cerevisiae, we have analyzed the influence ..
  11. Nakanishi S, Ueda T, Hori H, Yamazaki N, Okada N, Watanabe K. A UGU sequence in the anticodon loop is a minimum requirement for recognition by Escherichia coli tRNA-guanine transglycosylase. J Biol Chem. 1994;269:32221-5 pubmed
    ..Our results suggest that the enzyme recognizes the U33-G34-U35 sequence in the anti-codon loop and not the tertiary structure of tRNA itself. ..
  12. Curnow A, Kung F, Koch K, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: gross tRNA structural requirements for recognition. Biochemistry. 1993;32:5239-46 pubmed
    tRNA-guanine transglycosylase (TGT) is the enzyme responsible for the post-transcriptional modification of specific tRNAs (those for Asn, Asp, His, and Tyr) with the hypermodified base, queuine...
  13. Yagi S, Yagi Tanaka K, Yoshioka J, Suzuki M. Expression enhancement of the Tn5 neomycin-resistance gene by removal of upstream ATG sequences and its use for probing heterologous upstream activating sequences in yeast. Curr Genet. 1993;24:12-20 pubmed
    ..coli DNA sequences that clearly function as UASs in yeast cells. Two of these prokaryotic sequences with UAS activity were identified as a part of the coding region of the tgt and the hydG gene, respectively.
  14. Deshpande K, Katze J. Characterization of cDNA encoding the human tRNA-guanine transglycosylase (TGT) catalytic subunit. Gene. 2001;265:205-12 pubmed
    ..Q by the base-for-base exchange of queuine (Q base) for guanine in the unmodified tRNA, a reaction catalyzed by TGT. A search of the human EST database for sequences with significant homology to the well studied TGT from ..
  15. Urbonavicius J, Qian Q, Durand J, Hagervall T, Bj rk G. Improvement of reading frame maintenance is a common function for several tRNA modifications. EMBO J. 2001;20:4863-73 pubmed publisher
    ..However, since the frequency of frameshifting in several different mutants [mnmA, mnmE, tgt, truA (hisT), trmD, miaA, miaB and miaE] defective in tRNA modification was higher compared with the corresponding ..
  16. Bereswill S, Fassbinder F, Völzing C, Haas R, Reuter K, Ficner R, et al. Cloning and functional characterization of the genes encoding 3-dehydroquinate synthase (aroB) and tRNA-guanine transglycosylase (tgt) from Helicobacter pylori. Med Microbiol Immunol. 1997;186:125-34 pubmed
    ..and genes present in databases, whereas the second was found to share a significant degree of homology with the tgt gene encoding tRNA-guanine transglycosylase from a variety of other bacteria (40-50% identity and 60-70% similarity)..
  17. Kung F, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: recognition of full-length 'queuine-cognate' tRNAs. FEBS Lett. 1998;431:427-32 pubmed
    ..in the incorporation of the modified base queuine into tRNA (position 34) is tRNA-guanine transglycosylase (TGT)...
  18. Garcia G, Tierney D, Chong S, Clark K, Penner Hahn J. X-ray absorption spectroscopy of the zinc site in tRNA-guanine transglycosylase from Escherichia coli. Biochemistry. 1996;35:3133-9 pubmed
    ..This reaction is catalyzed by tRNA-guanine transglycosylase (TGT). We have previously shown that the E. coli TGT is a zinc metalloprotein [Chong et al...
  19. Mueller S, Slany R. Structural analysis of the interaction of the tRNA modifying enzymes Tgt and QueA with a substrate tRNA. FEBS Lett. 1995;361:259-64 pubmed
    The enzymes tRNA guanine-transglycosylase (Tgt) and S-adenosylmethionine :tRNA ribosyltransferase-isomerase (QueA) participate in the biosynthesis of the hypermodified tRNA nucleoside queuosine (Q) in Escherichia coli...
  20. Noguchi S, Nishimura Y, Hirota Y, Nishimura S. Isolation and characterization of an Escherichia coli mutant lacking tRNA-guanine transglycosylase. Function and biosynthesis of queuosine in tRNA. J Biol Chem. 1982;257:6544-50 pubmed
    ..The defective gene, named tgt, was mapped at about 9 min on the E. coli chromosome, and the gene order was shown to be phoB-tgt-tsx...
  21. Slany R, Kersten H. The promoter of the tgt/sec operon in Escherichia coli is preceded by an upstream activation sequence that contains a high affinity FIS binding site. Nucleic Acids Res. 1992;20:4193-8 pubmed
    The tgt/sec operon in E. coli consists of five genes: queA, tgt, ORF12, secD, and secF. QueA and Tgt participate in the biosynthesis of the hypermodified t-RNA nucleoside Queuosine, whereas SecD and SecF are involved in protein secretion...
  22. Hoops G, Townsend L, Garcia G. tRNA-guanine transglycosylase from Escherichia coli: structure-activity studies investigating the role of the aminomethyl substituent of the heterocyclic substrate PreQ1. Biochemistry. 1995;34:15381-7 pubmed
    ..have been synthesized in order to study the substrate specificity of the tRNA-guanine transglycosylase (TGT) from Escherichia coli...
  23. Reuter K, Chong S, Ullrich F, Kersten H, Garcia G. Serine 90 is required for enzymic activity by tRNA-guanine transglycosylase from Escherichia coli. Biochemistry. 1994;33:7041-6 pubmed
    ..tRNA lacking the hypermodified wobble nucleoside queuosine (Q) due to an inactive tRNA-guanine transglycosylase (TGT)...