Gene Symbol: tdh
Description: L-threonine 3-dehydrogenase, NAD(P)-binding
Alias: ECK3606, JW3591
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Boylan S, Dekker E. L-threonine dehydrogenase. Purification and properties of the homogeneous enzyme from Escherichia coli K-12. J Biol Chem. 1981;256:1809-15 pubmed
    ..43 and 0.19 mM, respectively. The enzyme has a pH optimum of 10.3, is activated by Mn2+, and shows a substantial loss of activity when treated with certain sulfhydryl-reacting reagents. ..
  2. Craig P, Dekker E. Cd2+ activation of L-threonine dehydrogenase from Escherichia coli K-12. Biochim Biophys Acta. 1988;957:222-9 pubmed
    ..Km values for L-threonine and NAD+ as well as the Vmax for 'demetallized', Cd2+-activated, and Mn2+-activated threonine dehydrogenase were determined and compared. ..
  3. Epperly B, Dekker E. L-threonine dehydrogenase from Escherichia coli. Identification of an active site cysteine residue and metal ion studies. J Biol Chem. 1991;266:6086-92 pubmed
    ..coli as a member of the zinc-containing long chain alcohol/polyol dehydrogenases; it is unique among these enzymes in that its activity is stimulated by Mn2+ or Cd2+. ..
  4. Chen Y, Dekker E, Somerville R. Functional analysis of E. coli threonine dehydrogenase by means of mutant isolation and characterization. Biochim Biophys Acta. 1995;1253:208-14 pubmed
    ..coli threonine dehydrogenase, 35 alleles of tdh that imparted a no-growth or slow-growth phenotype on appropriate indicator media were isolated after mutagenesis ..
  5. Craig P, Dekker E. L-threonine dehydrogenase from Escherichia coli K-12: thiol-dependent activation by Mn2+. Biochemistry. 1986;25:1870-6 pubmed
    ..A theoretical curve fit to data for the pH-activity profile of Mn2+-saturated enzyme has a pKa = 7.95 for one proton ionization. The data establish L-threonine dehydrogenase of E. coli to be a metal ion activated enzyme. ..
  6. Epperly B, Dekker E. Inactivation of Escherichia coli L-threonine dehydrogenase by 2,3-butanedione. Evidence for a catalytically essential arginine residue. J Biol Chem. 1989;264:18296-301 pubmed
    ..L. (1962) Sci. Sin. 11, 1535-1558) demonstrates that inactivation of threonine dehydrogenase correlates with the loss of 1 "essential" arginine residue/subunit which quite likely is located in the NAD+/NADH binding site...
  7. Ravnikar P, Somerville R. Structural and functional analysis of a cloned segment of Escherichia coli DNA that specifies proteins of a C4 pathway of serine biosynthesis. J Bacteriol. 1987;169:4716-21 pubmed
    ..This suggests that at least one other protein encoded within pDR121 plays an essential role in the conversion of threonine to serine. ..
  8. Ravnikar P, Somerville R. Genetic characterization of a highly efficient alternate pathway of serine biosynthesis in Escherichia coli. J Bacteriol. 1987;169:2611-7 pubmed
    ..ser genes plus a second lesion in glyA (serine hydroxymethyltransferase), gcv (the glycine cleavage system), or tdh (threonine dehydrogenase)...
  9. Aronson B, Somerville R, Epperly B, Dekker E. The primary structure of Escherichia coli L-threonine dehydrogenase. J Biol Chem. 1989;264:5226-32 pubmed
    ..complete primary structures of Escherichia coli L-threonine dehydrogenase has been deduced by sequencing the cloned tdh gene...

More Information


  1. Ravnikar P, Somerville R. Localization of the structural gene for threonine dehydrogenase in Escherichia coli. J Bacteriol. 1986;168:434-6 pubmed
    The threonine dehydrogenase (tdh) gene of Escherichia coli, cloned within the plasmid pDR121, was inactivated in vitro by inserting a segment of DNA carrying the chloramphenicol acetyltransferase (cat) gene...
  2. Boylan S, Dekker E. L-Threonine dehydrogenase of Escherichia coli K-12. Biochem Biophys Res Commun. 1978;85:190-7 pubmed
  3. Kim Y, Park J, Cho J, Cho K, Park Y, Lee J. Proteomic response analysis of a threonine-overproducing mutant of Escherichia coli. Biochem J. 2004;381:823-9 pubmed
    ..coli mutant. ..
  4. Anjem A, Imlay J. Mononuclear iron enzymes are primary targets of hydrogen peroxide stress. J Biol Chem. 2012;287:15544-56 pubmed publisher
    ..The implication is that mononuclear metalloproteins are common targets of H(2)O(2) and that both structural and metabolic arrangements exist to protect them. ..
  5. Boylan S, Dekker E. Growth, enzyme levels, and some metabolic properties of an Escherichia coli mutant grown on L-threonine as the sole carbon source. J Bacteriol. 1983;156:273-80 pubmed
    ..SBD-76 cells grown on L-threonine excreted glycine and aminoacetone into the medium, and extracts of the mutant strain catalyzed a quantitative conversion of L-threonine to glycine and aminoacetone. ..
  6. Craig P, Dekker E. The sulfhydryl content of L-threonine dehydrogenase from Escherichia coli K-12: relation to catalytic activity and Mn2+ activation. Biochim Biophys Acta. 1990;1037:30-8 pubmed
    ..Specific alkylation of one -SH group/enzyme subunit apparently causes protein conformational changes that entail a loss of catalytic activity and the ability to bind Mn2+. ..
  7. Landgraf J, Levinthal M, Danchin A. The role of H-NS in one carbon metabolism. Biochimie. 1994;76:1063-70 pubmed
    ..We show that H-NS is involved in the transcriptional regulation of the kbl/tdh operon, which is also Lrp regulated...
  8. Marcus J, Dekker E. Threonine formation via the coupled activity of 2-amino-3-ketobutyrate coenzyme A lyase and threonine dehydrogenase. J Bacteriol. 1993;175:6505-11 pubmed
    ..A constructed strain of E. coli, MD901 (glyA thrB/C tdh), was unable to grow unless both glycine and threonine were added to defined rich medium...