Gene Symbol: sufD
Description: component of SufBCD Fe-S cluster assembly scaffold
Alias: ECK1677, JW1671, ynhC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Zheng M, Wang X, Templeton L, Smulski D, LaRossa R, Storz G. DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide. J Bacteriol. 2001;183:4562-70 pubmed
    ..These results expand our understanding of the oxidative stress response and raise interesting questions regarding the nature of other regulators that modulate gene expression in response to hydrogen peroxide. ..
  2. Takahashi Y, Tokumoto U. A third bacterial system for the assembly of iron-sulfur clusters with homologs in archaea and plastids. J Biol Chem. 2002;277:28380-3 pubmed
    ..The genes homologous to sufBC are present in a wide range of bacteria, Archaea, and plastids, suggesting that this type of system is almost ubiquitous in nature. ..
  3. Lee K, Yeo W, Roe J. Oxidant-responsive induction of the suf operon, encoding a Fe-S assembly system, through Fur and IscR in Escherichia coli. J Bacteriol. 2008;190:8244-7 pubmed publisher
    ..For apo-IscR to bind, oxidation-mediated dissociation of Fur is required. Therefore, a peroxide-responsive signal is transduced through OxyR, IscR, and Fur to achieve oxidation-sensitive and maximal induction of this operon. ..
  4. Layer G, Gaddam S, Ayala Castro C, Ollagnier de Choudens S, Lascoux D, Fontecave M, et al. SufE transfers sulfur from SufS to SufB for iron-sulfur cluster assembly. J Biol Chem. 2007;282:13342-50 pubmed
    ..The interaction occurs only if SufC is present. Furthermore, SufB can act as a site for Fe-S cluster assembly in the Suf system. This provides the first evidence of a novel site for Fe-S cluster assembly in the SufBCD complex. ..
  5. Johnson D, Dean D, Smith A, Johnson M. Structure, function, and formation of biological iron-sulfur clusters. Annu Rev Biochem. 2005;74:247-81 pubmed
  6. Wada K, Sumi N, Nagai R, Iwasaki K, Sato T, Suzuki K, et al. Molecular dynamism of Fe-S cluster biosynthesis implicated by the structure of the SufC(2)-SufD(2) complex. J Mol Biol. 2009;387:245-58 pubmed publisher
    ..This machinery is encoded in Escherichia coli by the sufABCDSE operon, where three Suf components, SufB, SufC, and SufD, form a complex and appear to provide an intermediary site for the Fe-S cluster assembly...
  7. Kang Y, Weber K, Qiu Y, Kiley P, Blattner F. Genome-wide expression analysis indicates that FNR of Escherichia coli K-12 regulates a large number of genes of unknown function. J Bacteriol. 2005;187:1135-60 pubmed publisher
    ..We suggest that E. coli MG1655 has a larger metabolic potential under anaerobic conditions than has been previously recognized...
  8. Nachin L, Loiseau L, Expert D, Barras F. SufC: an unorthodox cytoplasmic ABC/ATPase required for [Fe-S] biogenesis under oxidative stress. EMBO J. 2003;22:427-37 pubmed
    ..Of particular interest are the sufB, sufC and sufD genes, which are conserved among Eubacteria, Archaea, plants and parasites...
  9. Tokumoto U, Kitamura S, Fukuyama K, Takahashi Y. Interchangeability and distinct properties of bacterial Fe-S cluster assembly systems: functional replacement of the isc and suf operons in Escherichia coli with the nifSU-like operon from Helicobacter pylori. J Biochem. 2004;136:199-209 pubmed
    ..While the differences between ISC and SUF were small with respect to the complementing activity, the SUF system appears to be more advantageous for bacterial growth in the presence of hydrogen peroxide. ..

More Information


  1. Lee J, Yeo W, Roe J. Induction of the sufA operon encoding Fe-S assembly proteins by superoxide generators and hydrogen peroxide: involvement of OxyR, IHF and an unidentified oxidant-responsive factor. Mol Microbiol. 2004;51:1745-55 pubmed
  2. Wollers S, Layer G, Garcia Serres R, Signor L, Clemancey M, Latour J, et al. Iron-sulfur (Fe-S) cluster assembly: the SufBCD complex is a new type of Fe-S scaffold with a flavin redox cofactor. J Biol Chem. 2010;285:23331-41 pubmed publisher
    ..conditions, this process is achieved by the SUF system that contains six proteins as follows: SufA, SufB, SufC, SufD, SufS, and SufE...
  3. Patzer S, Hantke K. SufS is a NifS-like protein, and SufD is necessary for stability of the [2Fe-2S] FhuF protein in Escherichia coli. J Bacteriol. 1999;181:3307-9 pubmed
    Escherichia coli fhuF mutants, a sufS::MudI mutant, and a sufD::MudI mutant were found to have the same phenotype: the inability to use ferrioxamine B as an iron source in a plate assay...
  4. Badger J, Sauder J, Adams J, Antonysamy S, Bain K, Bergseid M, et al. Structural analysis of a set of proteins resulting from a bacterial genomics project. Proteins. 2005;60:787-96 pubmed
    ..More distant sequence-structure relationships between the SGX and PDB structures were investigated using PDB-BLAST and Combinatorial Extension (CE). Only one structure, SufD, has a truly unique topology compared to all folds in the PDB.
  5. Fontecave M, Choudens S, Py B, Barras F. Mechanisms of iron-sulfur cluster assembly: the SUF machinery. J Biol Inorg Chem. 2005;10:713-21 pubmed
  6. Barras F, Loiseau L, Py B. How Escherichia coli and Saccharomyces cerevisiae build Fe/S proteins. Adv Microb Physiol. 2005;50:41-101 pubmed
    ..It is our hope that this parallel coverage will facilitate the identification of both similarities and specificities of ISC and SUF systems. ..
  7. Yeo W, Lee J, Lee K, Roe J. IscR acts as an activator in response to oxidative stress for the suf operon encoding Fe-S assembly proteins. Mol Microbiol. 2006;61:206-18 pubmed
    ..These results support a model that IscR in its demetallated form directly activates sufA transcription, while it de-represses isc operon, under oxidative stress condition. ..
  8. Loiseau L, Ollagnier de Choudens S, Nachin L, Fontecave M, Barras F. Biogenesis of Fe-S cluster by the bacterial Suf system: SufS and SufE form a new type of cysteine desulfurase. J Biol Chem. 2003;278:38352-9 pubmed publisher
    ..SUF system is made of six proteins: SufC is an atypical cytoplasmic ABC-ATPase, which forms a complex with SufB and SufD; SufA plays the role of a scaffold protein for assembly of iron-sulfur clusters and delivery to target proteins; ..
  9. Chahal H, Dai Y, Saini A, Ayala Castro C, Outten F. The SufBCD Fe-S scaffold complex interacts with SufA for Fe-S cluster transfer. Biochemistry. 2009;48:10644-53 pubmed publisher
    ..Our results represent the first biochemical evidence that the SufBCD complex within the Suf pathway functions as a novel Fe-S scaffold system to assemble nascent clusters and transfer them to the SufA Fe-S shuttle. ..
  10. Djaman O, Outten F, Imlay J. Repair of oxidized iron-sulfur clusters in Escherichia coli. J Biol Chem. 2004;279:44590-9 pubmed
  11. Outten F, Wood M, Munoz F, Storz G. The SufE protein and the SufBCD complex enhance SufS cysteine desulfurase activity as part of a sulfur transfer pathway for Fe-S cluster assembly in Escherichia coli. J Biol Chem. 2003;278:45713-9 pubmed
    ..We also found that the SufB, SufC, and SufD proteins associate in a stable complex and that, in the presence of SufE, the SufBCD complex further stimulates ..
  12. Saini A, Mapolelo D, Chahal H, Johnson M, Outten F. SufD and SufC ATPase activity are required for iron acquisition during in vivo Fe-S cluster formation on SufB. Biochemistry. 2010;49:9402-12 pubmed publisher
    ..Although SufB, SufC, and SufD are all required for in vivo Suf function, their exact roles are unclear...