stx2A

Summary

Gene Symbol: stx2A
Description: shiga-like toxin II A subunit encoded by bacteriophage BP-933W
Species: Escherichia coli O157:H7 EDL933

Top Publications

  1. ncbi Cloning and nucleotide sequence of a variant Shiga-like toxin II gene from Escherichia coli OX3:H21 isolated from a case of sudden infant death syndrome
    A W Paton
    Department of Microbiology, Adelaide Children s Hospital, South Australia
    Microb Pathog 13:225-36. 1992
  2. ncbi Stx2 subtyping of Shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new Stx2 subtype and correlation with additional virulence factors
    Y Bertin
    Laboratoire de Microbiologie, Centre de Recherche INRA de Clermont Ferrand Theix, 63122 St Genes Champanelle, France
    J Clin Microbiol 39:3060-5. 2001
  3. ncbi Phylogenetic diversity and similarity of active sites of Shiga toxin (stx) in Shiga toxin-producing Escherichia coli (STEC) isolates from humans and animals
    H Asakura
    Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Epidemiol Infect 127:27-36. 2001
  4. ncbi Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli
    Udo Reischl
    Institute of Medical Microbiology and Hygiene, University of Regensburg, D 93053 Regensburg, Germany
    J Clin Microbiol 40:2555-65. 2002
  5. ncbi Genetic variation in the flanking regions of Shiga toxin 2 gene in Shiga toxin-producing Escherichia coli O157:H7 isolated in Japan
    Takashi Hamabata
    Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    FEMS Microbiol Lett 215:229-36. 2002
  6. ncbi Presence of activatable Shiga toxin genotype (stx(2d)) in Shiga toxigenic Escherichia coli from livestock sources
    Kari S Gobius
    Food Science Australia, Tingalpa DC, Queensland 4173, Australia
    J Clin Microbiol 41:3777-83. 2003
  7. ncbi Shiga toxin-producing Escherichia coli in Montana: bacterial genotypes and clinical profiles
    Jill K Jelacic
    University of Washington School of Medicine, Seattle, Washington, USA
    J Infect Dis 188:719-29. 2003
  8. ncbi A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli
    P H M Leung
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, People s Republic of China
    Appl Environ Microbiol 69:7549-53. 2003
  9. ncbi Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 279:27511-7. 2004
  10. ncbi Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis
    Liesbet De Baets
    Dienst Algemene Bacteriologie, Centrum voor Onderzoek in Diergeneeskunde en Agrochemie, Groeselenberg 99, B 1180 Ukkel, Belgium
    Appl Environ Microbiol 70:6309-14. 2004

Scientific Experts

  • Martina Bielaszewska
  • U Reischl
  • Kari S Gobius
  • Takashi Hamabata
  • Y Bertin
  • Cristina Garcia-Aljaro
  • Marie E Fraser
  • H Asakura
  • S Makino
  • Tsutomu Koitabashi
  • Wenlan Zhang
  • Liesbet De Baets
  • T Shirahata
  • T Ikeda
  • K Takeshi
  • Jill K Jelacic
  • P H M Leung
  • K A Bettelheim
  • V Ramachandran
  • T Bellin
  • D Pierard
  • H Kurazono
  • H Kobori
  • A W Paton
  • M Iwasa
  • T Tsukamoto
  • Son Radu
  • Norio Asai
  • Varaporn Vuddhakul
  • Mitsuaki Nishibuchi
  • Tadaaki Morigaki
  • Yoshitsugu Nakaguchi
  • Thorsten Kuczius
  • Alexander W Friedrich
  • Helge Karch
  • Jean Pierre Hernalsteens
  • Lesley Allison
  • Henri De Greve
  • Jean-Pierre Hernalsteens
  • S H Kim
  • Denis Pierard
  • Imme Van der Taelen
  • Marina De Filette
  • Hein Imberechts
  • W C Yam
  • Marcia Ciol
  • Angela R Melton-Celsa
  • Phillip I Tarr
  • R M Robins-Browne
  • J S M Peiris
  • Todd Damrow
  • Humberto M Carvalho
  • Srdjan Jelacic
  • Gilbert S Chen
  • W W S Ng
  • ALISON D O'BRIEN
  • M J Walker
  • M Watarai
  • H G Hempen
  • M Pulz
  • F Gunzer
  • M A Hornitzky
  • S P Djordjevic
  • A Matussek
  • P A Manning
  • S Franke
  • J C Paton
  • Y Morimoto
  • S Lauwers
  • D Stevens
  • G Muyldermans
  • L Moriau
  • Y C Lee
  • K S Kim
  • I H Cha
  • Y H Kim
  • H Karch
  • Z Lin
  • D Harmsen
  • A Caprioli
  • A J Bourne
  • L H Wieler
  • T Meyer
  • S Yamasaki
  • T Suganami
  • M Ohmura
  • S Sakamoto
  • T Karasawa
  • Y Taniguchi
  • T Inoue

Detail Information

Publications31

  1. ncbi Cloning and nucleotide sequence of a variant Shiga-like toxin II gene from Escherichia coli OX3:H21 isolated from a case of sudden infant death syndrome
    A W Paton
    Department of Microbiology, Adelaide Children s Hospital, South Australia
    Microb Pathog 13:225-36. 1992
    ..The B subunit was less similar, exhibiting 88.6 and 88.8% homology to the related gene at the DNA and amino acid level, respectively...
  2. ncbi Stx2 subtyping of Shiga toxin-producing Escherichia coli isolated from cattle in France: detection of a new Stx2 subtype and correlation with additional virulence factors
    Y Bertin
    Laboratoire de Microbiologie, Centre de Recherche INRA de Clermont Ferrand Theix, 63122 St Genes Champanelle, France
    J Clin Microbiol 39:3060-5. 2001
    ..In addition, Stx2-EDL933 and/or Stx2vh-b subtypes were highly associated with other putative virulence factors such as Stx1 and EspP extracellular serine protease, but not with EAST1 enterotoxin...
  3. ncbi Phylogenetic diversity and similarity of active sites of Shiga toxin (stx) in Shiga toxin-producing Escherichia coli (STEC) isolates from humans and animals
    H Asakura
    Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Epidemiol Infect 127:27-36. 2001
    ....
  4. ncbi Real-time fluorescence PCR assays for detection and characterization of Shiga toxin, intimin, and enterohemolysin genes from Shiga toxin-producing Escherichia coli
    Udo Reischl
    Institute of Medical Microbiology and Hygiene, University of Regensburg, D 93053 Regensburg, Germany
    J Clin Microbiol 40:2555-65. 2002
    ....
  5. ncbi Genetic variation in the flanking regions of Shiga toxin 2 gene in Shiga toxin-producing Escherichia coli O157:H7 isolated in Japan
    Takashi Hamabata
    Research Institute, International Medical Center of Japan, 1 21 1 Toyama, Shinjuku, Tokyo 162 8655, Japan
    FEMS Microbiol Lett 215:229-36. 2002
    ..These results suggest that the DNA sequences surrounding the stx(2) genes are preferably integrated by IS1203v in wild-type Shiga toxin-producing E. coli strains...
  6. ncbi Presence of activatable Shiga toxin genotype (stx(2d)) in Shiga toxigenic Escherichia coli from livestock sources
    Kari S Gobius
    Food Science Australia, Tingalpa DC, Queensland 4173, Australia
    J Clin Microbiol 41:3777-83. 2003
    ..Surveillance for STEC strains expressing activatable Stx2d Shiga toxin among clinical cases may indicate the significance of this toxin subtype to human health...
  7. ncbi Shiga toxin-producing Escherichia coli in Montana: bacterial genotypes and clinical profiles
    Jill K Jelacic
    University of Washington School of Medicine, Seattle, Washington, USA
    J Infect Dis 188:719-29. 2003
    ..033). Non-O157:H7 STEC are more heterogeneous and cause bloody diarrhea less frequently than do E. coli O157:H7. Bloody diarrhea cannot be attributed simply to the stx genotype of the infecting organism...
  8. ncbi A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli
    P H M Leung
    Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong SAR, People s Republic of China
    Appl Environ Microbiol 69:7549-53. 2003
    ..VT2g was cytotoxic for Vero and HeLa cells and was not activated by mouse intestinal mucus. The vt2g gene was detected in 3 of 409 (0.7%) bovine VTEC strains, including serotypes O2:H25, O2:H45 and Ont:H-...
  9. ncbi Structure of shiga toxin type 2 (Stx2) from Escherichia coli O157:H7
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada
    J Biol Chem 279:27511-7. 2004
    ..Any of these structural differences might result in different mechanisms of action of the two toxins and the development of hemolytic uremic syndrome upon exposure to Stx2...
  10. ncbi Genetic typing of shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis
    Liesbet De Baets
    Dienst Algemene Bacteriologie, Centrum voor Onderzoek in Diergeneeskunde en Agrochemie, Groeselenberg 99, B 1180 Ukkel, Belgium
    Appl Environ Microbiol 70:6309-14. 2004
    ..A phylogenetic tree, based on the nucleotide sequences of the toxin-encoding genes, demonstrates that stx(2) sequences with the same PvuII HaeIII HincII AccI type generally cluster together...
  11. ncbi Transcriptional analysis of genes encoding Shiga toxin 2 and its variants in Escherichia coli
    Wenlan Zhang
    Institut fur Hygiene, Universitatsklinikum Munster, Robert Koch Str 41, 48149 Munster, Germany
    Appl Environ Microbiol 71:558-61. 2005
    ..These isolates produced significantly less stx mRNA than Stx2d, Stx2e, Stx2c, or Stx2 secretors did. Standard screening procedures miss a significant subset of E. coli harboring stx(2) variants...
  12. ncbi Genetic characterization of Escherichia coli O157: H7/- strains carrying the stx2 gene but not producing Shiga toxin 2
    Tsutomu Koitabashi
    Graduate School of Medicine, Kyoto University, Japan
    Microbiol Immunol 50:135-48. 2006
    ..These results suggest that stx(2)-positive O157 strains that produce little or no Stx2 may be widely distributed in the Asian environment...
  13. ncbi Chromosomal dynamism in progeny of outbreak-related sorbitol-fermenting enterohemorrhagic Escherichia coli O157:NM
    Martina Bielaszewska
    Institut fur Hygiene, Universitat Munster, Robert Koch Str 41, 48149 Munster, Germany
    Appl Environ Microbiol 72:1900-9. 2006
    ..The variability of PFGE patterns for the progeny of a single colony must be considered when interpreting PFGE patterns in SF EHEC O157-associated outbreaks...
  14. ncbi Newly identified bacteriophages carrying the stx2g Shiga toxin gene isolated from Escherichia coli strains in polluted waters
    Cristina Garcia-Aljaro
    Departament de Microbiologia, Universitat de Barcelona, Barcelona, Spain
    FEMS Microbiol Lett 258:127-35. 2006
    ..This first description of stx2g-carrying bacteriophages enlarges the list of stx-carrying bacteriophages involved in horizontal transmission of stx genes in the environment...
  15. ncbi The common ovine Shiga toxin 2-containing Escherichia coli serotypes and human isolates of the same serotypes possess a Stx2d toxin type
    V Ramachandran
    Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
    J Clin Microbiol 39:1932-7. 2001
    ..These studies suggest that a specific stx(2) subtype(s) associates with serotype and may have important epidemiological implications for tracing sources of E. coli during outbreaks of STEC-associated diseases in humans...
  16. ncbi Rapid detection of enterohemorrhagic Escherichia coli by real-time PCR with fluorescent hybridization probes
    T Bellin
    Hannover Medical School, Institute of Medical Microbiology, 30625 Hannover, Germany
    J Clin Microbiol 39:370-4. 2001
    ..In a multiplex assay, the genes encoding Shiga toxin 1 and Shiga toxin 2 are detected in a single reaction capillary. A complete analysis of up to 32 samples takes about 45 min...
  17. ncbi Cloning and sequencing of a Shiga-like toxin II-related gene from Escherichia coli O157:H7 strain 7279
    T Meyer
    Institut für Hygiene und Mikrobiologie, Universitat, Wurzburg
    Zentralbl Bakteriol 276:176-88. 1992
    ..Immunological differences of SLT-IIvhc and SLT-II as well as their different toxicity to HeLa cells presumably resulted from the small deviations within the primary structure of the B subunit...
  18. ncbi Cloning and nucleotide sequencing of Vero toxin 2 variant genes from Escherichia coli O91:H21 isolated from a patient with the hemolytic uremic syndrome
    H Ito
    Department of Microbiology, Faculty of Medicine, Kyoto University, Japan
    Microb Pathog 8:47-60. 1990
    ..These results indicate that nucleotide sequences encoding a family of VT2-related toxins are present in various strains of E. coli and that the sequences of the genes for A subunits are better conserved than those of the B subunit genes...
  19. ncbi Cloning and sequencing of a Shiga-like toxin type II variant from Escherichia coli strain responsible for edema disease of swine
    D L Weinstein
    Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814 4799
    J Bacteriol 170:4223-30. 1988
    ..Iron did not suppress SLT-IIv or SLT-II production, in contrast with its effect on Shiga toxin/SLT-I. Therefore, the regulation of synthesis of SLT-IIv and SLT-II differs from that of Shiga toxin/SLT-I...
  20. ncbi Cloning and nucleotide sequence analysis of the genes determining verocytotoxin production in a porcine edema disease isolate of Escherichia coli
    C L Gyles
    Department of Veterinary Microbiology and Immunology, University of Guelph, Ontario, Canada
    Microb Pathog 5:419-26. 1988
    ..The deduced amino acid sequences of the processed A and B subunits had 94% and 84% similarity, respectively. These findings confirm the close genetic relationship between SLT-II and edema disease principle...
  21. ncbi Comparative toxicity and virulence of Escherichia coli clones expressing variant and chimeric Shiga-like toxin type II operons
    A W Paton
    Department of Microbiology, Women s and Children s Hospital, North Adelaide, South Australia
    Infect Immun 63:2450-8. 1995
    ..These findings raise the possibility that naturally occurring SLT-II sequence variations may impact directly on the capacity of a given SLT-producing E. coli strain to cause disease...
  22. ncbi Cloning and sequencing of two new Verotoxin 2 variant genes of Escherichia coli isolated from cases of human and bovine diarrhea
    Z Lin
    Department of Microbiology, Faculty of Medicine, Kyoto University, Japan
    Microbiol Immunol 37:451-9. 1993
    ..Search for these two new genes in other Verocytotoxin-producing E. coli strains resulted in the isolation of 2 strains carrying one of the new VT2 variant genes, one strain from Tokyo and the other from Canada...
  23. ncbi Polymerase chain reaction amplification, cloning and sequencing of variant Escherichia coli Shiga-like toxin type II operons
    A W Paton
    Department of Microbiology, Adelaide Children s Hospital, South Australia
    Microb Pathog 15:77-82. 1993
    ..These fragments were cloned and DNA sequence analysis identified further variations compared with published SLT-II sequences...
  24. ncbi Clonal relatedness of Shiga-like toxin-producing Escherichia coli O101 strains of human and porcine origin
    S Franke
    Institut für Hygiene und Mikrobiology, Universitat Wurzburg, Germany
    J Clin Microbiol 33:3174-8. 1995
    ..coli O101 infection of humans. For diagnostic purposes, we recommend the use of PCR primers and DNA probes complementary to slt-IIe to correctly identify such strains and to further evaluate their role in human diseases...
  25. ncbi Cloning and sequence analysis of another Shiga-like toxin IIe variant gene (slt-IIera) from an Escherichia coli R107 strain isolated from rabbit
    S H Kim
    College of Veterinary Medicine, Gyeongsang National University, Chin Ju, South Korea
    Microbiol Immunol 41:805-8. 1997
    ..Recombinant SLT-IIera and SLT-IIe produced using an E. coli host-vector system showed similar cytotoxicity, suggesting that the variations in the structural gene of SLT-IIera have no significant effect on cytotoxic level...
  26. ncbi Identification of new verocytotoxin type 2 variant B-subunit genes in human and animal Escherichia coli isolates
    D Pierard
    Department of Microbiology, VTEC Reference Laboratory, Academisch Ziekenhuis Vrije Universiteit Brussel, Brussels, Belgium
    J Clin Microbiol 36:3317-22. 1998
    ..The extended PCR-RFLP typing method is a useful tool to identify less-virulent VTEC isolates and for VT genotyping in epidemiological studies with non-O157 strains...
  27. ncbi Detection and long-term existence of Shiga toxin (Stx)-producing Escherichia coli in sheep
    H Asakura
    Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Microbiol Immunol 42:683-8. 1998
    ..These results demonstrate that sheep may be an important animal for studying human STEC infections, and that further epidemiological surveys on STEC are necessary...
  28. ncbi Detection and genetical characterization of Shiga toxin-producing Escherichia coli from wild deer
    H Asakura
    Department of Veterinary Microbiology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    Microbiol Immunol 42:815-22. 1998
    ..These results suggest that STEC contamination of deer carcasses should be considered as a potential source of human infection and adequate sanitary inspection of meat for human consumption is also essential for wild animals...
  29. ncbi Detection of Escherichia coli O157:H7 from Musca domestica (Diptera: Muscidae) at a cattle farm in Japan
    M Iwasa
    Laboratory of Entomology, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan
    J Med Entomol 36:108-12. 1999
    ..These results indicate that the house fly is capable of carrying the toxigenic EHEC O157:H7 involved in human disease...
  30. ncbi Binding of adenine to Stx2, the protein toxin from Escherichia coli O157:H7
    Marie E Fraser
    Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary AB T2N 1N4, Canada
    Acta Crystallogr Sect F Struct Biol Cryst Commun 62:627-30. 2006
    ..Adenosine had been cleaved, proving that Stx2 is an active N-glycosidase. While the holotoxin is active against small substrates, it would be expected that the B subunits would interfere with the binding of the 28S rRNA...