stpA

Summary

Gene Symbol: stpA
Description: DNA binding protein, nucleoid-associated
Alias: ECK2663, JW2644, hnsB, hsm-3, rsv
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Lucchini S, McDermott P, Thompson A, Hinton J. The H-NS-like protein StpA represses the RpoS (sigma 38) regulon during exponential growth of Salmonella Typhimurium. Mol Microbiol. 2009;74:1169-86 pubmed publisher
    b>StpA is a paralogue of the nucleoid-associated protein H-NS that is conserved in a range of enteric bacteria and had no known function in Salmonella Typhimurium...
  2. Cusick M, Belfort M. Domain structure and RNA annealing activity of the Escherichia coli regulatory protein StpA. Mol Microbiol. 1998;28:847-57 pubmed
    The Escherichia coli regulatory protein StpA bears striking similarity to the chromatin-associated protein H-NS. These two proteins have many structural, functional and mechanistic parallels...
  3. Azam T, Ishihama A. Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999;274:33105-13 pubmed
    ..of chromosome initiation A), IHF (integration host factor), Lrp (leucine-responsive regulatory protein), and StpA (suppressor of td(-) phenotype A)...
  4. Leonard P, Ono S, Gor J, Perkins S, Ladbury J. Investigation of the self-association and hetero-association interactions of H-NS and StpA from Enterobacteria. Mol Microbiol. 2009;73:165-79 pubmed publisher
    The nucleoid-associated protein H-NS and its paralogue StpA are global regulators of gene expression and form an integral part of the protein scaffold responsible for DNA condensation in Escherichia coli and Salmonella typhimurium...
  5. Keatch S, Leonard P, Ladbury J, Dryden D. StpA protein from Escherichia coli condenses supercoiled DNA in preference to linear DNA and protects it from digestion by DNase I and EcoKI. Nucleic Acids Res. 2005;33:6540-6 pubmed
    The nucleoid-associated protein, StpA, of Escherichia coli binds non-specifically to double-stranded DNA (dsDNA) and apparently forms bridges between adjacent segments of the DNA...
  6. Johansson J, Uhlin B. Differential protease-mediated turnover of H-NS and StpA revealed by a mutation altering protein stability and stationary-phase survival of Escherichia coli. Proc Natl Acad Sci U S A. 1999;96:10776-81 pubmed
    ..was the result of a mutation that led to severalfold higher levels of the functionally and structurally related StpA protein...
  7. Zhang A, Rimsky S, Reaban M, Buc H, Belfort M. Escherichia coli protein analogs StpA and H-NS: regulatory loops, similar and disparate effects on nucleic acid dynamics. EMBO J. 1996;15:1340-9 pubmed
    Expression of the Escherichia coli StpA protein was investigated and a functional comparison undertaken with the structurally analogous nucleoid protein H-NS...
  8. Uyar E, Kurokawa K, Yoshimura M, Ishikawa S, Ogasawara N, Oshima T. Differential binding profiles of StpA in wild-type and h-ns mutant cells: a comparative analysis of cooperative partners by chromatin immunoprecipitation-microarray analysis. J Bacteriol. 2009;191:2388-91 pubmed publisher
    We performed chromatin immunoprecipitation-microarray analysis to investigate differences in function between StpA and H-NS in Escherichia coli cells...
  9. Sonden B, Uhlin B. Coordinated and differential expression of histone-like proteins in Escherichia coli: regulation and function of the H-NS analog StpA. EMBO J. 1996;15:4970-80 pubmed
    ..We have examined the regulation of the stpA gene, which encodes a protein sharing 58% identity with H-NS, by mRNA analysis and by using stpA-lacZ operon ..

More Information

Publications49

  1. Waldsich C, Grossberger R, Schroeder R. RNA chaperone StpA loosens interactions of the tertiary structure in the td group I intron in vivo. Genes Dev. 2002;16:2300-12 pubmed
    ..Alternatively, folding of the pre-mRNA can be promoted by the RNA chaperone StpA or by the group I intron-specific splicing factor Cyt-18...
  2. Bertin P, Benhabiles N, Krin E, Laurent Winter C, Tendeng C, Turlin E, et al. The structural and functional organization of H-NS-like proteins is evolutionarily conserved in gram-negative bacteria. Mol Microbiol. 1999;31:319-29 pubmed
    ..g. secondary structure prediction and DNA binding domain modelling) of the amino acid sequence of H-NS, StpA (an H-NS-like protein in E. coli), BpH3 and HvrA and by in vivo and in vitro experiments (e.g...
  3. Ali Azam T, Iwata A, Nishimura A, Ueda S, Ishihama A. Growth phase-dependent variation in protein composition of the Escherichia coli nucleoid. J Bacteriol. 1999;181:6361-70 pubmed
    ..of chromosome initiation A), IHF (integration host factor), Lrp (leucine-responsive regulatory protein), and StpA (suppressor of td mutant phenotype A)...
  4. Zhang A, Derbyshire V, Salvo J, Belfort M. Escherichia coli protein StpA stimulates self-splicing by promoting RNA assembly in vitro. RNA. 1995;1:783-93 pubmed
    An Escherichia coli gene, stpA, has been identified and cloned based on its ability to suppress the Td- phenotype of a resident, splicing-defective phage T4 td (thymidylate synthase) gene. The stpA gene, which was localized to 60...
  5. Mayer O, Waldsich C, Grossberger R, Schroeder R. Folding of the td pre-RNA with the help of the RNA chaperone StpA. Biochem Soc Trans. 2002;30:1175-80 pubmed
    ..In order to understand the mechanism of action of RNA chaperones, we probed the impact of the RNA chaperone StpA on the structure of the td intron in vivo and compared it with that of the well characterized Cyt-18 protein, which ..
  6. Sonnenfield J, Burns C, Higgins C, Hinton J. The nucleoid-associated protein StpA binds curved DNA, has a greater DNA-binding affinity than H-NS and is present in significant levels in hns mutants. Biochimie. 2001;83:243-9 pubmed
    The StpA protein is closely related to H-NS, the well-characterised global regulator of gene expression which is a major component of eubacterial chromatin...
  7. Johansson J, Eriksson S, Sonden B, Wai S, Uhlin B. Heteromeric interactions among nucleoid-associated bacterial proteins: localization of StpA-stabilizing regions in H-NS of Escherichia coli. J Bacteriol. 2001;183:2343-7 pubmed
    The nucleoid-associated proteins H-NS and StpA in Escherichia coli bind DNA as oligomers and are implicated in gene regulatory systems. There is evidence for both homomeric and heteromeric H-NS-StpA complexes...
  8. Shi X, Bennett G. Plasmids bearing hfq and the hns-like gene stpA complement hns mutants in modulating arginine decarboxylase gene expression in Escherichia coli. J Bacteriol. 1994;176:6769-75 pubmed
    ..2 min on the E. coli chromosome. We identified an open reading frame (stpA) with a deduced amino acid sequence showing more than 60% identity with the sequences of H-NS proteins from several ..
  9. Free A, Dorman C. The Escherichia coli stpA gene is transiently expressed during growth in rich medium and is induced in minimal medium and by stress conditions. J Bacteriol. 1997;179:909-18 pubmed
    The transcriptional regulation of the stpA gene, encoding the Escherichia coli H-NS-like protein StpA, has been studied as a function of a variety of environmental conditions, and its response to trans-acting factors has been ..
  10. Azam T, Hiraga S, Ishihama A. Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid. Genes Cells. 2000;5:613-26 pubmed
    ..The group-I proteins, including the major nucleoid-structural proteins, H-NS, HU, IHF, StpA and Dps, are distributed uniformly within the entire nucleoid...
  11. Clodi E, Semrad K, Schroeder R. Assaying RNA chaperone activity in vivo using a novel RNA folding trap. EMBO J. 1999;18:3776-82 pubmed
    ..However, known RNA chaperones such as Escherichia coli StpA and S12 and the HIV protein NCp7, only resolve the exonic trap without suppressing intron mutations...
  12. Zhang A, Belfort M. Nucleotide sequence of a newly-identified Escherichia coli gene, stpA, encoding an H-NS-like protein. Nucleic Acids Res. 1992;20:6735 pubmed
  13. Grossberger R, Mayer O, Waldsich C, Semrad K, Urschitz S, Schroeder R. Influence of RNA structural stability on the RNA chaperone activity of the Escherichia coli protein StpA. Nucleic Acids Res. 2005;33:2280-9 pubmed
    ..structural stabilities derived from the thymidylate synthase (td) group I intron and measured the effect of StpA, an Escherichia coli protein with RNA chaperone activity, on their splicing activity in vivo and in vitro...
  14. Dorman C, Hinton J, Free A. Domain organization and oligomerization among H-NS-like nucleoid-associated proteins in bacteria. Trends Microbiol. 1999;7:124-8 pubmed
    The bacterial nucleoid-associated proteins H-NS and StpA can form homomeric or heteromeric complexes, a parallel with protein HU...
  15. Free A, Porter M, Deighan P, Dorman C. Requirement for the molecular adapter function of StpA at the Escherichia coli bgl promoter depends upon the level of truncated H-NS protein. Mol Microbiol. 2001;42:903-17 pubmed
    ..Previous studies have provided evidence for the involvement of either the homologous nucleoid protein StpA or the alternative sigma factor RpoS in this unusual silencing mechanism...
  16. Rajkowitsch L, Schroeder R. Dissecting RNA chaperone activity. RNA. 2007;13:2053-60 pubmed
    ..coli, host factor Hfq, ribosomal protein S1, and the histone-like protein StpA for their abilities to promote two simple reactions, RNA annealing and strand displacement...
  17. Srinivasan R, Chandraprakash D, Krishnamurthi R, Singh P, Scolari V, Krishna S, et al. Genomic analysis reveals epistatic silencing of "expensive" genes in Escherichia coli K-12. Mol Biosyst. 2013;9:2021-33 pubmed publisher
    ..The activity of H-NS is enhanced or diminished by other proteins including its homologue StpA, and Hha and YdgT...
  18. Wolf T, Janzen W, Blum C, Schnetz K. Differential dependence of StpA on H-NS in autoregulation of stpA and in regulation of bgl. J Bacteriol. 2006;188:6728-38 pubmed
    b>StpA has functional similarity to its homologue, the nucleoid structuring protein H-NS. It binds to AT-rich, planar, bent DNA and constrains DNA supercoils. In addition, StpA acts as an RNA chaperone. StpA and H-NS also form heterodimers...
  19. Johansson J, Balsalobre C, Wang S, Urbonaviciene J, Jin D, Sonden B, et al. Nucleoid proteins stimulate stringently controlled bacterial promoters: a link between the cAMP-CRP and the (p)ppGpp regulons in Escherichia coli. Cell. 2000;102:475-85 pubmed
    ..Bacteria lacking both H-NS and the paralog StpA show reduced growth rate...
  20. Guo F, Adhya S. Spiral structure of Escherichia coli HUalphabeta provides foundation for DNA supercoiling. Proc Natl Acad Sci U S A. 2007;104:4309-14 pubmed
  21. Riber L, Olsson J, Jensen R, Skovgaard O, Dasgupta S, Marinus M, et al. Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome. Genes Dev. 2006;20:2121-34 pubmed
    ..Based on these observations, we propose that both RIDA and dnaA gene autoregulation are required as homeostatic mechanisms to ensure that initiation of replication occurs at the same time relative to cell mass in each cell cycle. ..
  22. Müller C, Dobrindt U, Nagy G, Emody L, Uhlin B, Hacker J. Role of histone-like proteins H-NS and StpA in expression of virulence determinants of uropathogenic Escherichia coli. J Bacteriol. 2006;188:5428-38 pubmed
    ..However, no comprehensive study on the role of H-NS and its paralogue, StpA, in gene expression in pathogenic E. coli has been carried out so far...
  23. Zimmerman S. Cooperative transitions of isolated Escherichia coli nucleoids: implications for the nucleoid as a cellular phase. J Struct Biol. 2006;153:160-75 pubmed
    ..in nucleoids isolated from cells after exposure to chloramphenicol or from cells in which Dps, Fis, or H-NS and StpA had been deleted...
  24. Brambilla L, Morán Barrio J, Viale A. Expression of the Escherichia coli ompW colicin S4 receptor gene is regulated by temperature and modulated by the H-NS and StpA nucleoid-associated proteins. FEMS Microbiol Lett. 2014;352:238-44 pubmed publisher
    ..Also, ?hns?stpA double mutants simultaneously lacking H-NS and its paralog StpA showed more severe reductions in ompW expression at ..
  25. Rouquette C, Serre M, Lane D. Protective role for H-NS protein in IS1 transposition. J Bacteriol. 2004;186:2091-8 pubmed
  26. Deighan P, Free A, Dorman C. A role for the Escherichia coli H-NS-like protein StpA in OmpF porin expression through modulation of micF RNA stability. Mol Microbiol. 2000;38:126-39 pubmed
    When a wild-type strain of Escherichia coli and its stpA, hns and stpA hns mutant derivatives were compared by two-dimensional protein gel electrophoresis, the levels of expression of several proteins were found to vary...
  27. Lim C, Whang Y, Kenney L, Yan J. Gene silencing H-NS paralogue StpA forms a rigid protein filament along DNA that blocks DNA accessibility. Nucleic Acids Res. 2012;40:3316-28 pubmed publisher
    ..Whereas the DNA-binding mechanism of H-NS is well-characterized, its paralogue, StpA which is also able to silence genes is less understood...
  28. Free A, Williams R, Dorman C. The StpA protein functions as a molecular adapter to mediate repression of the bgl operon by truncated H-NS in Escherichia coli. J Bacteriol. 1998;180:994-7 pubmed
    ..The DNA-binding function of the H-NS-like protein StpA was found to be necessary for repression, which is consistent with a role for StpA as a DNA-binding adapter for ..
  29. Neusser T, Gildehaus N, Wurm R, Wagner R. Studies on the expression of 6S RNA from E. coli: involvement of regulators important for stress and growth adaptation. Biol Chem. 2008;389:285-97 pubmed publisher
    ..aim, we have analyzed the effects of the typical bacterial growth phase and stress regulators FIS, H-NS, LRP and StpA on 6S RNA expression...
  30. Ikeda H, Shiraishi K, Ogata Y. Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli. Adv Biophys. 2004;38:3-20 pubmed
    ..Many features of these models remain to be tested in future studies. ..
  31. Bertin P, Hommais F, Krin E, Soutourina O, Tendeng C, Derzelle S, et al. H-NS and H-NS-like proteins in Gram-negative bacteria and their multiple role in the regulation of bacterial metabolism. Biochimie. 2001;83:235-41 pubmed
    ..Finally, expression profiling and phenotypic analysis suggest that, unlike H-NS, its paralogous protein StpA does not play any role in these processes.
  32. Shiraishi K, Ogata Y, Hanada K, Kano Y, Ikeda H. Roles of the DNA binding proteins H-NS and StpA in homologous recombination and repair of bleomycin-induced damage in Escherichia coli. Genes Genet Syst. 2007;82:433-9 pubmed
    The DNA binding protein H-NS promotes homologous recombination in Escherichia coli, but the role of its paralog StpA in this process remains unclear...
  33. Weber H, Pesavento C, Possling A, Tischendorf G, Hengge R. Cyclic-di-GMP-mediated signalling within the sigma network of Escherichia coli. Mol Microbiol. 2006;62:1014-34 pubmed
  34. Paytubi S, Madrid C, Forns N, Nieto J, Balsalobre C, Uhlin B, et al. YdgT, the Hha paralogue in Escherichia coli, forms heteromeric complexes with H-NS and StpA. Mol Microbiol. 2004;54:251-63 pubmed
    ..In addition to the hns gene, the chromosome of E. coli and other enteric bacteria also includes the stpA gene that encodes the StpA protein, an H-NS paralogue. We report here the identification of the Hha paralogue in E...
  35. Gueuné H, Durand M, Thouand G, DuBow M. The ygaVP genes of Escherichia coli form a tributyltin-inducible operon. Appl Environ Microbiol. 2008;74:1954-8 pubmed publisher
    ..We demonstrate that transcription from the promoter upstream of ygaVP is constitutive in a ygaVP mutant, suggesting that YgaV is an autoregulated, TBT-inducible repressor. ..
  36. Williams R, Rimsky S, Buc H. Probing the structure, function, and interactions of the Escherichia coli H-NS and StpA proteins by using dominant negative derivatives. J Bacteriol. 1996;178:4335-43 pubmed
    ..b>StpA is a protein with known structural and functional homologies to H-NS...
  37. Delihas N, Forst S. MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors. J Mol Biol. 2001;313:1-12 pubmed
    ..In addition, other factors, such as the RNA chaperone protein StpA also play a role in this regulatory system...
  38. Johansson J, Dagberg B, Richet E, Uhlin B. H-NS and StpA proteins stimulate expression of the maltose regulon in Escherichia coli. J Bacteriol. 1998;180:6117-25 pubmed
    ..Here we report findings showing that H-NS and the largely homologous protein StpA play a positive role in the expression of genes in the maltose regulon...
  39. Mayer O, Rajkowitsch L, Lorenz C, Konrat R, Schroeder R. RNA chaperone activity and RNA-binding properties of the E. coli protein StpA. Nucleic Acids Res. 2007;35:1257-69 pubmed
    The E. coli protein StpA has RNA annealing and strand displacement activities and it promotes folding of RNAs by loosening their structures...
  40. Talukder A, Ishihama A. Growth phase dependent changes in the structure and protein composition of nucleoid in Escherichia coli. Sci China Life Sci. 2015;58:902-11 pubmed publisher
    ..protein from starved cells (Dps), host factor for phage Qβ (Hfq) and suppressor of td(-) phenotype A (StpA)...