Gene Symbol: selB
Description: selenocysteinyl-tRNA-specific translation factor
Alias: ECK3579, JW3563, fdhA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Thanbichler M, Bock A. The function of SECIS RNA in translational control of gene expression in Escherichia coli. EMBO J. 2002;21:6925-34 pubmed
    ..the triplet coding for selenocysteine, and they tether a complex of the selenocysteine-specific elongation factor SelB, GTP and selenocysteyl-tRNA(Sec) to the site of UGA decoding...
  2. Bock A, Hilgenfeld R, Tormay P, Wilting R, Kromayer M. Domain structure of the selenocysteine-specific translation factor SelB in prokaryotes. Biomed Environ Sci. 1997;10:125-8 pubmed
    Translation factor SelB is the key component for the specific decoding of UGA codons with selenocysteine at the ribosome...
  3. Heider J, Baron C, Bock A. Coding from a distance: dissection of the mRNA determinants required for the incorporation of selenocysteine into protein. EMBO J. 1992;11:3759-66 pubmed
    ..of a particular codon inside an mRNA with selenocysteine and a model is proposed in which translation factor SELB interacts with this recognition sequence, thus forming a quaternary complex at the mRNA together with GTP and ..
  4. Li C, Reches M, Engelberg Kulka H. The bulged nucleotide in the Escherichia coli minimal selenocysteine insertion sequence participates in interaction with SelB: a genetic approach. J Bacteriol. 2000;182:6302-7 pubmed
    ..coli fdhF SECIS is involved in the in vivo interaction with SelB. We used a genetic approach, generating and characterizing selB mutations that suppress mutations of the bulged ..
  5. Thanbichler M, Bock A. Functional analysis of prokaryotic SELB proteins. Biofactors. 2001;14:53-9 pubmed
    ..Elongation factor SELB, whose amino-terminal part shows homology to EF-Tu, was found to be the key component mediating delivery of ..
  6. Chen G, Fang L, Inouye M. Effect of the relative position of the UGA codon to the unique secondary structure in the fdhF mRNA on its decoding by selenocysteinyl tRNA in Escherichia coli. J Biol Chem. 1993;268:23128-31 pubmed
    ..codon is decoded by selenocysteinyl tRNA (the selC product) with the aid of its own specific elongation factor, SelB. For this decoding, a unique secondary structure immediately downstream of the UGA codon has been shown to be ..
  7. Leinfelder W, Forchhammer K, Zinoni F, Sawers G, Mandrand Berthelot M, Bock A. Escherichia coli genes whose products are involved in selenium metabolism. J Bacteriol. 1988;170:540-6 pubmed
    ..They were analyzed, together with previously characterized pleiotropic fdh mutants (fdhA, fdhB, and fdhC), for their ability to incorporate selenium into the selenopolypeptide subunits of FDHN and FDHH...
  8. Thanbichler M, Bock A, Goody R. Kinetics of the interaction of translation factor SelB from Escherichia coli with guanosine nucleotides and selenocysteine insertion sequence RNA. J Biol Chem. 2000;275:20458-66 pubmed
    The kinetics of the interaction of GTP and GDP with SelB, the specific translation factor for the incorporation of selenocysteine into proteins, have been investigated using the stopped-flow method...
  9. Huttenhofer A, Westhof E, Bock A. Solution structure of mRNA hairpins promoting selenocysteine incorporation in Escherichia coli and their base-specific interaction with special elongation factor SELB. RNA. 1996;2:354-66 pubmed
    ..for fdhF mRNA) located in a bulge 5' to the loop, are involved directly in binding to special elongation factor SELB in both mRNAs...

More Information


  1. Baron C, Heider J, Bock A. Interaction of translation factor SELB with the formate dehydrogenase H selenopolypeptide mRNA. Proc Natl Acad Sci U S A. 1993;90:4181-5 pubmed
    The SELB protein from Escherichia coli is a specialized elongation factor required for the UGA-directed insertion of the amino acid selenocysteine into selenopolypeptides...
  2. Kraal B, Bosch L, Mesters J, de Graaf J, Woudt L, Vijgenboom E, et al. Elongation factors in protein synthesis. Ciba Found Symp. 1993;176:28-43; discussion 44-52 pubmed
    ..We have found a synergistic effect between EF-Tu and EF-G in their uncoupled GTPase reactions on empty ribosomes. Only the EF-G reaction is perturbed by fluoroaluminates. ..
  3. Zinoni F, Birkmann A, Stadtman T, Bock A. Nucleotide sequence and expression of the selenocysteine-containing polypeptide of formate dehydrogenase (formate-hydrogen-lyase-linked) from Escherichia coli. Proc Natl Acad Sci U S A. 1986;83:4650-4 pubmed
    ..The results are consistent with a co-translational selenocysteine incorporation mechanism. ..
  4. Forchhammer K, R├╝cknagel K, Bock A. Purification and biochemical characterization of SELB, a translation factor involved in selenoprotein synthesis. J Biol Chem. 1990;265:9346-50 pubmed
    The product of the selB gene from Escherichia coli is required for co-translational insertion of selenocysteine into protein...
  5. Soler N, Fourmy D, Yoshizawa S. Crystallization and preliminary X-ray analysis of the mRNA-binding domain of elongation factor SelB from Escherichia coli in complex with RNA. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:419-21 pubmed
    In bacteria, selenocysteine (the 21st amino acid) is incorporated into proteins via machinery that includes SelB, a specific translational elongation factor...
  6. Bock A, Forchhammer K, Heider J, Baron C. Selenoprotein synthesis: an expansion of the genetic code. Trends Biochem Sci. 1991;16:463-7 pubmed
    ..In E. coli, this requires a specific translation factor. Selenocysteine thus represents a unique expansion of the genetic code. ..
  7. Stadtman T. Biosynthesis and function of selenocysteine-containing enzymes. J Biol Chem. 1991;266:16257-60 pubmed
  8. Hilgenfeld R, Bock A, Wilting R. Structural model for the selenocysteine-specific elongation factor SelB. Biochimie. 1996;78:971-8 pubmed
    A structural model was established for the N-terminal part of translation factor SelB which shares sequence similarity with EF-Tu, taking into account the coordinates of the EF-Tu 3D structure and the consensus of SelB sequences from four ..
  9. Kozmin S, Schaaper R. Molybdenum cofactor-dependent resistance to N-hydroxylated base analogs in Escherichia coli is independent of MobA function. Mutat Res. 2007;619:9-15 pubmed
    ..Further, we establish that protection against the analogs does not require the common molybdopterin guanine-dinucleotide (MGD) form of the cofactor, but instead the guanosine monophosphate (GMP)-free version of MoCo (MPT) is sufficient. ..
  10. Soler N, Fourmy D, Yoshizawa S. Molecular switch in tandem winged-helix motifs of elongation factor SelB. Nucleic Acids Symp Ser (Oxf). 2007;:377-8 pubmed
    Elongation factor SelB is responsible for cotranslational incorporation of the 21(st) amino acid selenocysteine (Sec) into proteins. UGA stop codon is recoded as a Sec codon in presence of a downstream mRNA hairpin...
  11. Yu H, Kim K. The involvement of SelB in the expression of cytotoxic necrotizing factor 1 in Escherichia coli. FEBS Lett. 2011;585:1934-40 pubmed publisher
    ..Mini-Tn5 mutagenesis of meningitis-causing E. coli revealed that transposon mutants of selA and selB genes failed to express CNF1...
  12. Bianco P, Kowalczykowski S. The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'. Proc Natl Acad Sci U S A. 1997;94:6706-11 pubmed
    ..This implies that recognition of Chi occurs following the unwinding of the DNA. ..
  13. Ringquist S, Schneider D, Gibson T, Baron C, Bock A, Gold L. Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB. Genes Dev. 1994;8:376-85 pubmed
    ..Incorporation of selenocysteine relies, in part, on the interaction between a specialized elongation factor, the SELB protein, and a cis-acting element within the mRNA...
  14. Baron C, Bock A. The length of the aminoacyl-acceptor stem of the selenocysteine-specific tRNA(Sec) of Escherichia coli is the determinant for binding to elongation factors SELB or Tu. J Biol Chem. 1991;266:20375-9 pubmed
    ..analyzed for their interaction with purified seryl-tRNA synthetase, selenocysteine synthase and translation factors SELB and EF-Tu...
  15. Huttenhofer A, Bock A. Selenocysteine inserting RNA elements modulate GTP hydrolysis of elongation factor SelB. Biochemistry. 1998;37:885-90 pubmed
    Elongation factor SelB is required for the incorporation of the amino acid selenocysteine into proteins in Escherichia coli...
  16. Kromayer M, Wilting R, Tormay P, B ck A. Domain structure of the prokaryotic selenocysteine-specific elongation factor SelB. J Mol Biol. 1996;262:413-20 pubmed publisher
    ..of the non-canonical amino acid selenocysteine into proteins requires the activity of the elongation factor SelB which substitutes for the function of EF-Tu in contrast to EF-Tu, SelB binds selenocystylated tRNASec and an mRNA ..
  17. Rengby O, Johansson L, Carlson L, Serini E, Vlamis Gardikas A, Karsnas P, et al. Assessment of production conditions for efficient use of Escherichia coli in high-yield heterologous recombinant selenoprotein synthesis. Appl Environ Microbiol. 2004;70:5159-67 pubmed
    ..of a secondary structure in the mRNA forming a selenocysteine insertion sequence (SECIS) element compatible with SelB, the elongation factor for selenocysteine insertion at a predefined UGA codon...
  18. Leinfelder W, Stadtman T, Bock A. Occurrence in vivo of selenocysteyl-tRNA(SERUCA) in Escherichia coli. Effect of sel mutations. J Biol Chem. 1989;264:9720-3 pubmed
    ..The formation of selenocysteine depended on the presence of functional products of the selA and selD genes but not of the selB gene. The selB gene product, therefore, may have a function in the decoding step itself.
  19. Soler N, Fourmy D, Yoshizawa S. Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB. J Mol Biol. 2007;370:728-41 pubmed publisher
    Elongation factor SelB is responsible for co-translational incorporation of selenocysteine (Sec) into proteins. The UGA stop codon is recoded as a Sec codon in the presence of a downstream mRNA hairpin...
  20. Paleskava A, Konevega A, Rodnina M. Thermodynamic and kinetic framework of selenocysteyl-tRNASec recognition by elongation factor SelB. J Biol Chem. 2010;285:3014-20 pubmed publisher
    b>SelB is a specialized translation elongation factor that delivers selenocysteyl-tRNA(Sec) (Sec-tRNA(Sec)) to the ribosome. Here we show that Sec-tRNA(Sec) binds to SelB.GTP with an extraordinary high affinity (K(d) = 0.2 pm)...
  21. Kromayer M, Neuhierl B, Friebel A, Bock A. Genetic probing of the interaction between the translation factor SelB and its mRNA binding element in Escherichia coli. Mol Gen Genet. 1999;262:800-6 pubmed
    ..of the UGA codon in mRNAs for selenoproteins as selenocysteine requires interaction of the translation factor SelB with an mRNA structure, the SECIS element...
  22. Fagegaltier D, Carbon P, Krol A. Distinctive features in the SelB family of elongation factors for selenoprotein synthesis. A glimpse of an evolutionary complexified translation apparatus. Biofactors. 2001;14:5-10 pubmed
    ..or eukaryotes, this incorporation requires several gene products, among which the specialized elongation factor SelB and the tRNA(Sec) play a pivotal role...
  23. Forchhammer K, Leinfelder W, Bock A. Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein. Nature. 1989;342:453-6 pubmed
    ..By contrast, the selB gene product (SELB) is not required until after selenocysteyl-tRNA biosynthesis...
  24. Sandman K, Tardiff D, Neely L, Noren C. Revised Escherichia coli selenocysteine insertion requirements determined by in vivo screening of combinatorial libraries of SECIS variants. Nucleic Acids Res. 2003;31:2234-41 pubmed
    ..These results suggest an unexpected downstream sequence flexibility in prokaryotic selenoprotein expression. ..
  25. Sawers G, Heider J, Zehelein E, Bock A. Expression and operon structure of the sel genes of Escherichia coli and identification of a third selenium-containing formate dehydrogenase isoenzyme. J Bacteriol. 1991;173:4983-93 pubmed
    ..The selA and selB genes formed one transcriptional unit (sel vector AB), while selD was shown to be the central gene in an operon ..
  26. Forchhammer K, Boesmiller K, Bock A. The function of selenocysteine synthase and SELB in the synthesis and incorporation of selenocysteine. Biochimie. 1991;73:1481-6 pubmed
    The selAB operon codes for the proteins selenocysteine synthase and SELB which catalyse the synthesis and cotranslational insertion of selenocysteine into protein...