secM

Summary

Gene Symbol: secM
Description: regulator of secA translation
Alias: ECK0098, JW5007, srrA, yacA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Schmidt M, Oliver D. SecA protein autogenously represses its own translation during normal protein secretion in Escherichia coli. J Bacteriol. 1989;171:643-9 pubmed
    ..Furthermore, overexpression of the SecA protein severely reduced expression of only the secA-lacZ protein fusion, indicating that SecA autogenously represses its own translation. ..
  2. Muto H, Nakatogawa H, Ito K. Genetically encoded but nonpolypeptide prolyl-tRNA functions in the A site for SecM-mediated ribosomal stall. Mol Cell. 2006;22:545-52 pubmed
    The arrest sequence, FXXXXWIXXXXGIRAGP, of E. coli SecM interacts with the ribosomal exit tunnel, thereby interfering with translation elongation. Here, we studied this elongation arrest in vitro using purified translation components...
  3. Garza Sánchez F, Janssen B, Hayes C. Prolyl-tRNA(Pro) in the A-site of SecM-arrested ribosomes inhibits the recruitment of transfer-messenger RNA. J Biol Chem. 2006;281:34258-68 pubmed
    ..binding site (A-site) mRNA cleavage occurs during regulatory translational pausing using the Escherichia coli SecM-mediated ribosome arrest as a model...
  4. Sunohara T, Jojima K, Tagami H, Inada T, Aiba H. Ribosome stalling during translation elongation induces cleavage of mRNA being translated in Escherichia coli. J Biol Chem. 2004;279:15368-75 pubmed
    ..We focused on a specific peptide sequence (AS17), derived from SecM, that is known to cause elongation arrest...
  5. Fikes J, Bassford P. Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide. J Bacteriol. 1989;171:402-9 pubmed
    ..The properties of these suppressor and insertion mutations provide some insight into the role of SecA in the protein export process...
  6. Nakatogawa H, Murakami A, Mori H, Ito K. SecM facilitates translocase function of SecA by localizing its biosynthesis. Genes Dev. 2005;19:436-44 pubmed
    quot;Arrest sequence" of Escherichia coli SecM interacts with the ribosomal exit tunnel and arrests its own translation elongation, which is released by cotranslational export of the nascent SecM chain...
  7. Sarker S, Oliver D. Critical regions of secM that control its translation and secretion and promote secretion-specific secA regulation. J Bacteriol. 2002;184:2360-9 pubmed
    ..A novel mechanism that promotes this regulation involves translational pausing within the gene upstream of secA, secM. The secM translational pause prevents formation of an RNA helix that normally blocks secA translational initiation...
  8. Ha H, Yeom J, Song W, Jeon C, Hahn Y, Lee K. Identification of a hyperactive variant of the SecM motif involved in ribosomal arrest. Curr Microbiol. 2012;64:17-23 pubmed publisher
    ..Amino acid sequences present at the C-terminal part of Escherichia coli SecM ((150)FSTPVWISQAQGIRAGP(166)) have a well-characterized role in ribosome stalling...
  9. Beall B, Lutkenhaus J. Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli. J Bacteriol. 1987;169:5408-15 pubmed
    ..Transcription of envA and the upstream fts genes terminated at this terminator and was probably uncoupled from the downstream genes, including secA. Gene disruption experiments indicated that the envA gene was an essential gene. ..

More Information

Publications37

  1. Contreras Martinez L, DeLisa M. Intracellular ribosome display via SecM translation arrest as a selection for antibodies with enhanced cytosolic stability. J Mol Biol. 2007;372:513-24 pubmed
    ..a novel method for intracellular ribosome display that takes advantage of the recently discovered Escherichia coli SecM translation arrest mechanism...
  2. Butkus M, Prundeanu L, Oliver D. Translocon "pulling" of nascent SecM controls the duration of its translational pause and secretion-responsive secA regulation. J Bacteriol. 2003;185:6719-22 pubmed
    ..In Escherichia coli SecA levels are regulated by the secretion needs of the cell utilizing secM, which encodes a secreted protein...
  3. Collier J, Bohn C, Bouloc P. SsrA tagging of Escherichia coli SecM at its translation arrest sequence. J Biol Chem. 2004;279:54193-201 pubmed
    b>SecM is expressed from the secM-secA operon and activates the expression of secA in response to secretion defects...
  4. Tanner D, Cariello D, Woolstenhulme C, Broadbent M, Buskirk A. Genetic identification of nascent peptides that induce ribosome stalling. J Biol Chem. 2009;284:34809-18 pubmed publisher
    ..We identified three classes of stalling peptides: C-terminal Pro residues, SecM-like peptides, and the novel stalling sequence FXXYXIWPP...
  5. Oliver D, Norman J, Sarker S. Regulation of Escherichia coli secA by cellular protein secretion proficiency requires an intact gene X signal sequence and an active translocon. J Bacteriol. 1998;180:5240-2 pubmed
    ..Furthermore, gene X signal sequence mutations disrupted secA regulation only in the cis configuration. We propose that nascent pre-gene X protein interacts with the translocon during its secretion to constitute the secretion sensor. ..
  6. Schmidt M, Brosh R, Oliver D. Escherichia coli SecA helicase activity is not required in vivo for efficient protein translocation or autogenous regulation. J Biol Chem. 2001;276:37076-85 pubmed
    ..SecA has also been shown to be an autogenous repressor that binds to its translation initiation region on secM-secA mRNA, thereby blocking and dissociating 30 S ribosomal subunits...
  7. Fortin Y, Phoenix P, Drapeau G. Mutations conferring resistance to azide in Escherichia coli occur primarily in the secA gene. J Bacteriol. 1990;172:6607-10 pubmed
    ..That azide inhibits SecA is consistent with the fact that SecA has ATPase activity, an activity that is often prone to inhibition by azide. ..
  8. Salavati R, Oliver D. Identification of elements on GeneX-secA RNA of Escherichia coli required for SecA binding and secA auto-regulation. J Mol Biol. 1997;265:142-52 pubmed
    ..Finally, it was shown that SecA binding was not sufficient to promote auto-regulation and that sequences both upstream (helix I) and within the binding site can contribute to auto-regulation without affecting SecA-binding affinity. ..
  9. Nakatogawa H, Ito K. The ribosomal exit tunnel functions as a discriminating gate. Cell. 2002;108:629-36 pubmed
    Translation of SecM stalls unless its N-terminal part is "pulled" by the protein export machinery...
  10. Nakatogawa H, Ito K. Intraribosomal regulation of expression and fate of proteins. Chembiochem. 2004;5:48-51 pubmed
    Our studies of SecM (secretion monitor) in E...
  11. Tian H, Beckwith J. Genetic screen yields mutations in genes encoding all known components of the Escherichia coli signal recognition particle pathway. J Bacteriol. 2002;184:111-8 pubmed
    ..5S RNA, and FtsY, respectively. In addition, the screen yielded mutations in secM, which is involved in regulating levels of the SecA component of the bacterium's protein export pathway...
  12. Nakatogawa H, Ito K. Secretion monitor, SecM, undergoes self-translation arrest in the cytosol. Mol Cell. 2001;7:185-92 pubmed
    The product of the Escherichia coli secM gene (secretion monitor, formerly gene X), upstream of secA, is involved in secretion-responsive control of SecA translation...
  13. Schmidt M, Rollo E, Grodberg J, Oliver D. Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli. J Bacteriol. 1988;170:3404-14 pubmed
    ..This and additional evidence are presented indicating that gene X and secA are cotranscribed. ..
  14. McNicholas P, Salavati R, Oliver D. Dual regulation of Escherichia coli secA translation by distinct upstream elements. J Mol Biol. 1997;265:128-41 pubmed
    ..Our results suggest strongly that there is a system of dual regulation of secA translation, whereby helix I serves as an activator element while helix II serves as a repressor element. ..
  15. Yap M, Bernstein H. The translational regulatory function of SecM requires the precise timing of membrane targeting. Mol Microbiol. 2011;81:540-53 pubmed publisher
    In Escherichia coli, secA expression is regulated at the translational level by an upstream gene (secM) that encodes a presecretory protein. SecM contains a C-terminal sequence motif that induces a transient translation arrest...
  16. Woolhead C, Johnson A, Bernstein H. Translation arrest requires two-way communication between a nascent polypeptide and the ribosome. Mol Cell. 2006;22:587-98 pubmed
    When the export of E. coli SecM is blocked, a 17 amino acid motif near the C terminus of the protein induces a translation arrest from within the ribosome tunnel...
  17. Rajapandi T, Dolan K, Oliver D. The first gene in the Escherichia coli secA operon, gene X, encodes a nonessential secretory protein. J Bacteriol. 1991;173:7092-7 pubmed
    ..Analysis of this strain indicated that gene X is nonessential for cell growth and viability and does not appear to play an essential role in the process of protein export. ..
  18. van der Sluis E, Driessen A. Stepwise evolution of the Sec machinery in Proteobacteria. Trends Microbiol. 2006;14:105-8 pubmed
    ..was initially supplemented with SecB; subsequently, SecE was extended with two transmembrane segments and, finally, SecM was introduced...
  19. Murakami A, Nakatogawa H, Ito K. Translation arrest of SecM is essential for the basal and regulated expression of SecA. Proc Natl Acad Sci U S A. 2004;101:12330-5 pubmed
    The SecM protein of Escherichia coli contains an arrest sequence (F(150)XXXXWIXXXXGIRAGP(166)), which interacts with the ribosomal exit tunnel to halt translation elongation beyond Pro-166...
  20. Beringer M. Modulating the activity of the peptidyl transferase center of the ribosome. RNA. 2008;14:795-801 pubmed publisher
    ..Another way by which the function of the PT center is controlled is signaling from the peptide exit tunnel. The SecM nascent peptide induces ribosome stalling, presumably by inhibition of peptide bond formation...
  21. Nakatogawa H, Murakami A, Ito K. Control of SecA and SecM translation by protein secretion. Curr Opin Microbiol. 2004;7:145-50 pubmed
    SecA, the protein translocation ATPase of E. coli is subject to secretion-defect-response control. SecM (secretion monitor) encoded by the 5' region of the secM-secA mRNA is involved in this regulation...
  22. Schmidt M, Dolan K, Oliver D. Regulation of Escherichia coli secA mRNA translation by a secretion-responsive element. J Bacteriol. 1991;173:6605-11 pubmed
    ..This region has been shown to bind SecA protein in vitro. These studies open the way for a direct investigation of the mechanism of secA regulation and its coupling to the protein secretion capability of the cell. ..
  23. Mitra K, Schaffitzel C, Fabiola F, Chapman M, Ban N, Frank J. Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements. Mol Cell. 2006;22:533-43 pubmed
    In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit...
  24. Luirink J, von Heijne G, Houben E, de Gier J. Biogenesis of inner membrane proteins in Escherichia coli. Annu Rev Microbiol. 2005;59:329-55 pubmed
    ..This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. ..
  25. Evans M, Ugrinov K, Frese M, Clark P. Homogeneous stalled ribosome nascent chain complexes produced in vivo or in vitro. Nat Methods. 2005;2:757-62 pubmed
    ..bearing nascent chains of a specified length by using a 'stall sequence', derived from the Escherichia coli SecM protein, which interacts with residues in the ribosomal exit tunnel to stall SecM translation...
  26. Sarker S, Rudd K, Oliver D. Revised translation start site for secM defines an atypical signal peptide that regulates Escherichia coli secA expression. J Bacteriol. 2000;182:5592-5 pubmed
    ..Escherichia coli secA occurs by coupling its translation to the translation and secretion of an upstream regulator, secM (formerly geneX)...
  27. Yap M, Bernstein H. The plasticity of a translation arrest motif yields insights into nascent polypeptide recognition inside the ribosome tunnel. Mol Cell. 2009;34:201-11 pubmed publisher
    The recognition of a C-terminal motif in E. coli SecM ((150)FXXXXWIXXXXGIRAGP(166)) inside the ribosome tunnel causes translation arrest, but the mechanism of recognition is unknown...
  28. Goldman D, Kaiser C, Milin A, Righini M, Tinoco I, Bustamante C. Ribosome. Mechanical force releases nascent chain-mediated ribosome arrest in vitro and in vivo. Science. 2015;348:457-60 pubmed publisher
    ..The SecM protein arrests its own translation, and release of arrest at the translocon has been proposed to occur by ..