Gene Symbol: secE
Description: preprotein translocase membrane subunit
Alias: ECK3972, JW3944, mbrC, prlG
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Bieker K, Silhavy T. PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli. Cell. 1990;61:833-42 pubmed
    ..for genetic analysis show that two inner membrane components of the export machinery, PrlA (SecY) and PrlG (SecE), interact directly while catalyzing the translocation of secreted proteins across the cytoplasmic membrane of E...
  2. Matsuyama S, Akimaru J, Mizushima S. SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machinery. FEBS Lett. 1990;269:96-100 pubmed
    The secY and secE genes were individually cloned and placed under the control of the tac promoter on plasmids. Induction with isopropyl-beta-D-thiogalactopyranoside resulted in the overproduction of SecE, but not that of SecY...
  3. Pogliano K, Beckwith J. The Cs sec mutants of Escherichia coli reflect the cold sensitivity of protein export itself. Genetics. 1993;133:763-73 pubmed
    ..Cs mutations comprise the largest class of mutations affecting the membrane-localized Sec proteins SecD, SecE, SecF and SecY...
  4. Yi L, Jiang F, Chen M, Cain B, Bolhuis A, Dalbey R. YidC is strictly required for membrane insertion of subunits a and c of the F(1)F(0)ATP synthase and SecE of the SecYEG translocase. Biochemistry. 2003;42:10537-44 pubmed
    ..We found that membrane subunits of the F(0) sector of the F(1)F(0)ATP synthase and the SecE protein of the SecYEG translocase are highly dependent on YidC for membrane insertion, based on protease mapping ..
  5. Veenendaal A, van der Does C, Driessen A. The protein-conducting channel SecYEG. Biochim Biophys Acta. 2004;1694:81-95 pubmed
    ..The ribosome and other membrane proteins can associate with the protein-conducting channel complex and affect its activity or functionality. ..
  6. Facey S, Kuhn A. The sensor protein KdpD inserts into the Escherichia coli membrane independent of the Sec translocase and YidC. Eur J Biochem. 2003;270:1724-34 pubmed
    ..was extended by an epitope tag to 27 amino acid residues, the membrane insertion of this loop of KdpD depended on SecE and YidC...
  7. Smith M, Clemons W, DeMars C, Flower A. Modeling the effects of prl mutations on the Escherichia coli SecY complex. J Bacteriol. 2005;187:6454-65 pubmed
    ..Our results support and expand a model of Prl suppressor activity that proposes that all of the prlA and prlG alleles either destabilize the closed state of the channel or stabilize the open form...
  8. Lycklama A Nijeholt J, Wu Z, Driessen A. Conformational dynamics of the plug domain of the SecYEG protein-conducting channel. J Biol Chem. 2011;286:43881-90 pubmed publisher
    ..In contrast, binding and insertion of a ribosome-bound nascent membrane protein did not alter the plug conformation. These data indicate different plug dynamics depending on the ligand bound state of the SecYEG channel. ..
  9. van der Laan M, Nouwen N, Driessen A. SecYEG proteoliposomes catalyze the Deltaphi-dependent membrane insertion of FtsQ. J Biol Chem. 2004;279:1659-64 pubmed
    ..These data demonstrate that membrane protein insertion can be reconstituted with a minimal set of purified Sec components. ..

More Information


  1. Breyton C, Haase W, Rapoport T, Kuhlbrandt W, Collinson I. Three-dimensional structure of the bacterial protein-translocation complex SecYEG. Nature. 2002;418:662-5 pubmed
    ..the channel is formed by oligomers of a heterotrimeric membrane protein complex consisting of subunits SecY, SecE and SecG. In the endoplasmic reticulum membrane, the channel is formed from the related Sec61 complex...
  2. de Gier J, Luirink J. Biogenesis of inner membrane proteins in Escherichia coli. Mol Microbiol. 2001;40:314-22 pubmed
    ..Here, we review and discuss recent advances in our understanding of the biogenesis of inner membrane proteins in E. coli. ..
  3. Bostina M, Mohsin B, Kuhlbrandt W, Collinson I. Atomic model of the E. coli membrane-bound protein translocation complex SecYEG. J Mol Biol. 2005;352:1035-43 pubmed
    ..The contacts arising at the dimer interface in the environment of the lipid bilayer may have activated the assembly. ..
  4. Manting E, van der Does C, Remigy H, Engel A, Driessen A. SecYEG assembles into a tetramer to form the active protein translocation channel. EMBO J. 2000;19:852-61 pubmed
    ..The mass and structure of this SecYEG as well as the subunit stoichiometry of SecA and SecY in a soluble translocase-precursor complex reveal that translocase consists of the SecA homodimer and four SecYEG complexes. ..
  5. Bieker K, Phillips G, Silhavy T. The sec and prl genes of Escherichia coli. J Bioenerg Biomembr. 1990;22:291-310 pubmed
    ..Subsequent characterization reveals that in at least three cases, prlA/secY, prlD/secA, and prlG/secE, both types of mutations are found within the same structural gene...
  6. Rusch S, Kendall D. Oligomeric states of the SecA and SecYEG core components of the bacterial Sec translocon. Biochim Biophys Acta. 2007;1768:5-12 pubmed
    ..This review focuses on what is known about the oligomeric states of these core Sec components and how the oligomeric state might change during the course of the translocation of a preprotein. ..
  7. van der Does C, Manting E, Kaufmann A, Lutz M, Driessen A. Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state. Biochemistry. 1998;37:201-10 pubmed
    Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain...
  8. Sugai M, Wu H. Export of the outer membrane lipoprotein is defective in secD, secE, and secF mutants of Escherichia coli. J Bacteriol. 1992;174:2511-6 pubmed
    The export of major outer membrane lipoprotein has been found to be affected in secD, secE, and secF mutants of Escherichia coli, which are defective in protein export in general...
  9. Angelini S, Deitermann S, Koch H. FtsY, the bacterial signal-recognition particle receptor, interacts functionally and physically with the SecYEG translocon. EMBO Rep. 2005;6:476-81 pubmed
    ..We now provide evidence for the direct interaction between FtsY and the SecY translocon. This interaction offers an explanation of how the bacterial SRP cycle is regulated in response to available translocation channels. ..
  10. Koch H, Hengelage T, Neumann Haefelin C, Macfarlane J, Hoffschulte H, Schimz K, et al. In vitro studies with purified components reveal signal recognition particle (SRP) and SecA/SecB as constituents of two independent protein-targeting pathways of Escherichia coli. Mol Biol Cell. 1999;10:2163-73 pubmed
    ..coli. Both targeting pathways intersect at the translocation pore because they are equally affected by a blocked translocation channel. ..
  11. du Plessis D, Nouwen N, Driessen A. Subunit a of cytochrome o oxidase requires both YidC and SecYEG for membrane insertion. J Biol Chem. 2006;281:12248-52 pubmed
    ..These data demonstrate that pre-CyoA is a substrate of a novel pathway that involves both SecYEG and YidC. ..
  12. Collinson I. The structure of the bacterial protein translocation complex SecYEG. Biochem Soc Trans. 2005;33:1225-30 pubmed
  13. Matsuo E, Mori H, Ito K. Interfering mutations provide in vivo evidence that Escherichia coli SecE functions in multimeric states. Mol Genet Genomics. 2003;268:808-15 pubmed
    SecY, SecE and SecG form a heterotrimer, which functions as a protein translocation channel in Escherichia coli. The cytosolic loop of SecE contains a segment that is conserved among different organisms...
  14. van der Laan M, Bechtluft P, Kol S, Nouwen N, Driessen A. F1F0 ATP synthase subunit c is a substrate of the novel YidC pathway for membrane protein biogenesis. J Cell Biol. 2004;165:213-22 pubmed
    ..In conclusion, a novel membrane protein insertion pathway in E. coli is described in which YidC plays an exclusive role. ..
  15. Pogliano J, Beckwith J. SecD and SecF facilitate protein export in Escherichia coli. EMBO J. 1994;13:554-61 pubmed
    ..Our results provide additional support for the notion that an early step in protein export is cold-sensitive. ..
  16. Bol R, de Wit J, Driessen A. The active protein-conducting channel of Escherichia coli contains an apolar patch. J Biol Chem. 2007;282:29785-93 pubmed
    ..Fluorescence measurements with defined proOmpA-DHFR translocation intermediates indicate mostly a water-exposed environment with a hydrophobic region in the center of the channel. ..
  17. Ojemalm K, Botelho S, Stüdle C, von Heijne G. Quantitative analysis of SecYEG-mediated insertion of transmembrane ?-helices into the bacterial inner membrane. J Mol Biol. 2013;425:2813-22 pubmed publisher
    ..coli inner membrane, but the hydrophobicity threshold for SecYEG-mediated insertion is distinctly lower than that for Sec61-mediated insertion. ..
  18. de Keyzer J, van der Does C, Driessen A. The bacterial translocase: a dynamic protein channel complex. Cell Mol Life Sci. 2003;60:2034-52 pubmed
    ..Here, we will discuss the mechanistic and structural basis of the translocation of proteins across and the integration of membrane proteins into the cytoplasmic membrane. ..
  19. Luirink J, von Heijne G, Houben E, de Gier J. Biogenesis of inner membrane proteins in Escherichia coli. Annu Rev Microbiol. 2005;59:329-55 pubmed
    ..This review deals mainly with recent structural and biochemical data from various experimental systems that offer new insight into the different stages of biogenesis of E. coli IMPs. ..
  20. Tian P, Bernstein H. Identification of a post-targeting step required for efficient cotranslational translocation of proteins across the Escherichia coli inner membrane. J Biol Chem. 2009;284:11396-404 pubmed publisher
    ..coli SecYEG complex does not ensure its export but that export also depends on a subsequent event (most likely the initiation of translocation) that involves sequences both within and just beyond the signal peptide. ..
  21. Harris C, Silhavy T. Mapping an interface of SecY (PrlA) and SecE (PrlG) by using synthetic phenotypes and in vivo cross-linking. J Bacteriol. 1999;181:3438-44 pubmed
    SecY and SecE are integral cytoplasmic membrane proteins that form an essential part of the protein translocation machinery in Escherichia coli...
  22. Moser M, Nagamori S, Huber M, Tokuda H, Nishiyama K. Glycolipozyme MPIase is essential for topology inversion of SecG during preprotein translocation. Proc Natl Acad Sci U S A. 2013;110:9734-9 pubmed publisher
    ..The dimer formation of SecG, crosslinked at TM 2, was not observed on SecG inversion, indicating that SecYEG undergoes a dynamic structural change during preprotein translocation. ..
  23. Gatsos X, Perry A, Anwari K, Dolezal P, Wolynec P, Likic V, et al. Protein secretion and outer membrane assembly in Alphaproteobacteria. FEMS Microbiol Rev. 2008;32:995-1009 pubmed publisher
  24. Bieker K, Silhavy T. The genetics of protein secretion in E. coli. Trends Genet. 1990;6:329-34 pubmed
    ..The picture emerging provides a useful paradigm for similar pathways in other organisms. ..
  25. Riggs P, Derman A, Beckwith J. A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene. Genetics. 1988;118:571-9 pubmed
    ..A mutation in one of these genes causes a pleiotropic defect in protein export and a cold-sensitive growth defect; this gene, which maps at approximately 90 min on the bacterial chromosome, has been named secE.
  26. Pugsley A, Kornacker M, Poquet I. The general protein-export pathway is directly required for extracellular pullulanase secretion in Escherichia coli K12. Mol Microbiol. 1991;5:343-52 pubmed
    ..In addition, we show here that six sec genes (secA, secB, secD, secE, secF and secY) are all required for processing of the prepullulanase signal peptide to occur...
  27. Brundage L, Hendrick J, Schiebel E, Driessen A, Wickner W. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell. 1990;62:649-57 pubmed
    ..The SecY/E protein consists of SecY, SecE, and an additional polypeptide...
  28. Joly J, Leonard M, Wickner W. Subunit dynamics in Escherichia coli preprotein translocase. Proc Natl Acad Sci U S A. 1994;91:4703-7 pubmed
    SecY, SecE, and band 1 copurify as the SecY/E integral membrane domain of Escherichia coli preprotein translocase...
  29. Shiomi D, Yoshimoto M, Homma M, Kawagishi I. Helical distribution of the bacterial chemoreceptor via colocalization with the Sec protein translocation machinery. Mol Microbiol. 2006;60:894-906 pubmed
    ..Observation of GFP-SecE and a translocation-defective MalE-GFP mutant, as well as indirect immunofluorescence microscopy on SecG, suggested ..
  30. Homma T, Yoshihisa T, Ito K. Subunit interactions in the Escherichia coli protein translocase: SecE and SecG associate independently with SecY. FEBS Lett. 1997;408:11-5 pubmed
    We used hexahistidine-tagged SecE and SecY to study how the core subunits (SecY, SecE and SecG) of Escherichia coli protein translocase interact with each other...
  31. Sullivan S, Ward D, Gottesman M. Effect of Escherichia coli nusG function on lambda N-mediated transcription antitermination. J Bacteriol. 1992;174:1339-44 pubmed
    ..These results suggest that NusG may act as a component of the N antitermination complex. In addition, the data imply a role for NusG in Rho-dependent termination. ..
  32. Flower A, Doebele R, Silhavy T. PrlA and PrlG suppressors reduce the requirement for signal sequence recognition. J Bacteriol. 1994;176:5607-14 pubmed
    ..These genes, secY/prlA and secE/prlG, encode integral membrane components of the protein translocation system of Escherichia coli...
  33. Estevenon A, Lemonnier M, Rouquette C, Lane D. Genetic basis of the MbrC "ploidy" phenotype in Escherichia coli. Mol Gen Genet. 1997;256:291-7 pubmed indicated that the mbrC17 mutation is closely linked to rpoB, and allele exchange showed it to lie within the secE-nusG operon...
  34. Hanada M, Nishiyama K, Tokuda H. SecG plays a critical role in protein translocation in the absence of the proton motive force as well as at low temperature. FEBS Lett. 1996;381:25-8 pubmed
    ..Protein translocation into proteoliposomes in the absence of the proton motive force thus required SecG whereas that in cells, which always generate the proton motive force, did not. ..
  35. Schatz P, Bieker K, Ottemann K, Silhavy T, Beckwith J. One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery. EMBO J. 1991;10:1749-57 pubmed
    The E. coli secE (prlG) gene codes for an integral cytoplasmic membrane protein which is part of the cell's secretory machinery...
  36. Valent Q, Scotti P, High S, de Gier J, von Heijne G, Lentzen G, et al. The Escherichia coli SRP and SecB targeting pathways converge at the translocon. EMBO J. 1998;17:2504-12 pubmed
    ..Hence, as appears to be the case for several other translocation systems, multiple targeting mechanisms deliver a variety of precursor proteins to a common membrane translocation complex of the E.coli inner membrane. ..
  37. Downing W, Sullivan S, Gottesman M, Dennis P. Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon. J Bacteriol. 1990;172:1621-7 pubmed
    Two genes, secE and nusG, situated between the tufB and ribosomal protein rplKAJL operons in the rif region at 90 min on the Escherichia coli chromosome, have been sequenced and characterized...
  38. Liang F, Bageshwar U, Musser S. Bacterial Sec protein transport is rate-limited by precursor length: a single turnover study. Mol Biol Cell. 2009;20:4256-66 pubmed publisher
    ..Instead, we propose that precursor movement results predominantly from Brownian motion and that the SecA ATPase regulates pore accessibility. ..
  39. Trun N, Gottesman S, Løbner Olesen A. Analysis of Escherichia coli mutants with altered DNA content. Cold Spring Harb Symp Quant Biol. 1991;56:353-8 pubmed
  40. Knyazev D, Lents A, Krause E, Ollinger N, Siligan C, Papinski D, et al. The bacterial translocon SecYEG opens upon ribosome binding. J Biol Chem. 2013;288:17941-6 pubmed publisher
    ..Thus, the open probability of the channel must be close to unity. To prevent the otherwise lethal proton leak, a closed post-translational conformation of the SecYEG complex bound to a ribosome must exist...
  41. Scheuring J, Braun N, Nothdurft L, Stumpf M, Veenendaal A, Kol S, et al. The oligomeric distribution of SecYEG is altered by SecA and translocation ligands. J Mol Biol. 2005;354:258-71 pubmed
    ..the peripheral membrane-associated ATPase SecA and the heterotrimeric channel-forming complex consisting of SecY, SecE and SecG. We have investigated the quaternary structure of the SecYEG complex in proteoliposomes...
  42. Tziatzios C, Schubert D, Lotz M, Gundogan D, Betz H, Schagger H, et al. The bacterial protein-translocation complex: SecYEG dimers associate with one or two SecA molecules. J Mol Biol. 2004;340:513-24 pubmed
    ..The observed variability in the stoichiometry of SecYEG and SecA association and its nucleotide modulation may be important and necessary for the protein translocation reaction. ..
  43. Akimaru J, Matsuyama S, Tokuda H, Mizushima S. Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli. Proc Natl Acad Sci U S A. 1991;88:6545-9 pubmed
    ..SecY was solubilized from SecY/SecE-overproducing Escherichia coli cells and purified by chromatography on ion-exchange and size-exclusion columns...
  44. Danese P, Murphy C, Silhavy T. Multicopy suppression of cold-sensitive sec mutations in Escherichia coli. J Bacteriol. 1995;177:4969-73 pubmed
    ..This multicopy suppression does not bypass the Sec pathway because a deletion of secE is not suppressed under these conditions...
  45. Nishiyama K, Mizushima S, Tokuda H. The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli. J Biol Chem. 1992;267:7170-6 pubmed
    Genes encoding the C- and N-terminal regions of SecE were constructed and placed under the control of the tac promoter on plasmids...
  46. Belin D, Plaia G, Boulfekhar Y, Silva F. Escherichia coli SecG is required for residual export mediated by mutant signal sequences and for SecY-SecE complex stability. J Bacteriol. 2015;197:542-52 pubmed publisher
    Protein export to the bacterial periplasm is achieved by SecYEG, an inner membrane heterotrimer. SecY and SecE are encoded by essential genes, while SecG is not essential for growth under standard laboratory conditions...
  47. Watkins W, Menon A. Reconstitution of phospholipid flippase activity from E. coli inner membrane: a test of the protein translocon as a candidate flippase. Biol Chem. 2002;383:1435-40 pubmed
    ..We conclude that the protein translocon contributes minimally, if at all, to phospholipid flippase activity in the inner membrane. ..
  48. du Plessis D, Berrelkamp G, Nouwen N, Driessen A. The lateral gate of SecYEG opens during protein translocation. J Biol Chem. 2009;284:15805-14 pubmed publisher
    ..The cross-linking data further suggests that SecYEG lateral gate opening and activation of the SecA ATPase are coupled processes. It is concluded that lateral gate opening is a critical step during SecA-dependent protein translocation. ..
  49. Nishiyama K, Mizushima S, Tokuda H. Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane. Biochem Biophys Res Commun. 1995;217:217-23 pubmed
    SecY, SecE and SecG form a membrane part of the protein translocation machinery. A SecG-overproducing plasmid was constructed by placing the secG gene under the control of the tac promoter...
  50. van der Sluis E, Nouwen N, Koch J, de Keyzer J, van der Does C, Tampe R, et al. Identification of two interaction sites in SecY that are important for the functional interaction with SecA. J Mol Biol. 2006;361:839-49 pubmed
    ..Our data explain how conformational changes in SecA could be directly coupled to the previously proposed opening mechanism of the SecYEG channel. ..
  51. Flower A, Osborne R, Silhavy T. The allele-specific synthetic lethality of prlA-prlG double mutants predicts interactive domains of SecY and SecE. EMBO J. 1995;14:884-93 pubmed
    ..from the cytoplasm of Escherichia coli requires the interaction of two integral inner membrane components, SecY and SecE. We have devised a genetic approach to probe the molecular nature of the SecY-SecE interaction...
  52. Bessonneau P, Besson V, Collinson I, Duong F. The SecYEG preprotein translocation channel is a conformationally dynamic and dimeric structure. EMBO J. 2002;21:995-1003 pubmed
    Escherichia coli preprotein translocase comprises a membrane-embedded trimeric complex of SecY, SecE and SecG...
  53. Flower A. SecG function and phospholipid metabolism in Escherichia coli. J Bacteriol. 2001;183:2006-12 pubmed
    ..It is hypothesized that this state of phospholipid imbalance imparts a dependence on SecG for proper function or stabilization of the translocation apparatus. ..
  54. Manting E, Driessen A. Escherichia coli translocase: the unravelling of a molecular machine. Mol Microbiol. 2000;37:226-38 pubmed
    ..Recent findings concerning the translocase structure and mechanism of protein translocation are discussed and shine new light on controversies in the field. ..
  55. Trun N, Gottesman S. Characterization of Escherichia coli mutants with altered ploidy. Res Microbiol. 1991;142:195-200 pubmed
    ..5 min; mbrC at 89.5 min; and mbrD at 90 min...
  56. Taura T, Baba T, Akiyama Y, Ito K. Determinants of the quantity of the stable SecY complex in the Escherichia coli cell. J Bacteriol. 1993;175:7771-5 pubmed
    ..Simultaneous overexpression of SecE led to complete stabilization of excess SecY...
  57. Zhu L, Wasey A, White S, Dalbey R. Charge composition features of model single-span membrane proteins that determine selection of YidC and SecYEG translocase pathways in Escherichia coli. J Biol Chem. 2013;288:7704-16 pubmed publisher
    ..These findings provide deeper insights into how pathways are selected for the insertion of proteins into the Escherichia coli inner membrane. ..
  58. Murphy C, Stewart E, Beckwith J. A double counter-selection system for the study of null alleles of essential genes in Escherichia coli. Gene. 1995;155:1-7 pubmed
    ..Using this system, we have (i) searched for bypass suppressors of a deletion of the essential secE gene, (ii) ascertained the ability of various mutant secE genes to complement a deletion of the wt copy and (iii) ..
  59. Duong F. Binding, activation and dissociation of the dimeric SecA ATPase at the dimeric SecYEG translocase. EMBO J. 2003;22:4375-84 pubmed
    ..The nucleotides and translocation-dependent changes of SecA-SecYEG associations and the SecA dimeric state may reflect important facets of the preprotein translocation reaction. ..
  60. Ito K, Wittekind M, Nomura M, Shiba K, Yura T, Miura A, et al. A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins. Cell. 1983;32:789-97 pubmed
    ..The gene secY is probably identical with prlA, previously identified as a suppressor of signal sequence mutations. ..
  61. Yahr T, Wickner W. Evaluating the oligomeric state of SecYEG in preprotein translocase. EMBO J. 2000;19:4393-401 pubmed
    ..i) Formaldehyde cross- linking of translocase reveals cross-links between SecY, SecE and SecG, but not higher order oligomers...
  62. Mori H, Akiyama Y, Ito K. A SecE mutation that modulates SecY-SecE translocase assembly, identified as a specific suppressor of SecY defects. J Bacteriol. 2003;185:948-56 pubmed
    ..A specific secE mutation, designated secE105, was thus isolated...
  63. Soman R, Yuan J, Kuhn A, Dalbey R. Polarity and charge of the periplasmic loop determine the YidC and sec translocase requirement for the M13 procoat lep protein. J Biol Chem. 2014;289:1023-32 pubmed publisher
    ..This study provides a framework to understand why the YidC and Sec machineries exist in parallel and demonstrates that the YidC insertase has a limited capacity to translocate a peptide chain on its own. ..
  64. van der Does C, Swaving J, van Klompenburg W, Driessen A. Non-bilayer lipids stimulate the activity of the reconstituted bacterial protein translocase. J Biol Chem. 2000;275:2472-8 pubmed
    ..Optimal activity required a mixture of anionic and non-bilayer lipids at concentrations that correspond to concentrations found in the natural membrane. ..
  65. Mandon E, Trueman S, Gilmore R. Translocation of proteins through the Sec61 and SecYEG channels. Curr Opin Cell Biol. 2009;21:501-7 pubmed publisher
  66. Nouwen N, Driessen A. SecDFyajC forms a heterotetrameric complex with YidC. Mol Microbiol. 2002;44:1397-405 pubmed
    ..As SecF and YajC have previously been shown to interact with SecY, we propose that these two proteins link the heterotetrameric SecDFyajC-YidC complex to the SecYEG complex...
  67. Antonoaea R, Fürst M, Nishiyama K, Muller M. The periplasmic chaperone PpiD interacts with secretory proteins exiting from the SecYEG translocon. Biochemistry. 2008;47:5649-56 pubmed publisher
    ..Consistent with a coupling between translocation across the SecYEG translocon and folding by periplasmic chaperones, a lack of PpiD retards the release of a translocating outer membrane protein into the periplasm. ..
  68. Xie K, Hessa T, Seppälä S, Rapp M, von Heijne G, Dalbey R. Features of transmembrane segments that promote the lateral release from the translocase into the lipid phase. Biochemistry. 2007;46:15153-61 pubmed
    ..coli system. These results are significant because they define the features of a membrane protein transmembrane segment that induce lateral release from the YidC and Sec translocases into the lipid bilayer in bacteria. ..
  69. Veenendaal A, van der Does C, Driessen A. Mapping the sites of interaction between SecY and SecE by cysteine scanning mutagenesis. J Biol Chem. 2001;276:32559-66 pubmed
    ..Both genetic and biochemical data indicate interactions of several transmembrane segments (TMSs) of SecY with SecE. By means of cysteine scanning mutagenesis, we have identified intermolecular sites of contact between TMS7 of SecY ..
  70. van der Sluis E, Driessen A. Stepwise evolution of the Sec machinery in Proteobacteria. Trends Microbiol. 2006;14:105-8 pubmed
    ..The canonical Sec machinery was initially supplemented with SecB; subsequently, SecE was extended with two transmembrane segments and, finally, SecM was introduced...
  71. Natale P, Den Blaauwen T, van der Does C, Driessen A. Conformational state of the SecYEG-bound SecA probed by single tryptophan fluorescence spectroscopy. Biochemistry. 2005;44:6424-32 pubmed
    ..In addition, SecYEG-bound SecA undergoes ATP-dependent conformational changes that are not observed for soluble SecA. These data support a model in which binding to the SecYEG channel has a major impact on the SecA conformation. ..
  72. Natale P, Swaving J, van der Does C, de Keyzer J, Driessen A. Binding of SecA to the SecYEG complex accelerates the rate of nucleotide exchange on SecA. J Biol Chem. 2004;279:13769-77 pubmed
    ..This defines a novel pretranslocation activated state of SecA that is primed for ATP hydrolysis upon preprotein interaction. ..
  73. Muller M, Koch H, Beck K, Schafer U. Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane. Prog Nucleic Acid Res Mol Biol. 2001;66:107-57 pubmed
    ..The majority are exported through pores formed by SecY and SecE. Two different molecular machineries are used to target proteins to the SecYE translocon...
  74. Douville K, Leonard M, Brundage L, Nishiyama K, Tokuda H, Mizushima S, et al. Band 1 subunit of Escherichia coli preportein translocase and integral membrane export factor P12 are the same protein. J Biol Chem. 1994;269:18705-7 pubmed
    ..isolated chromatographically or by immunoprecipitation, was found to be complex of three polypeptides, SecY, SecE, and band 1. Band 1 did not correspond to a known sec gene product...
  75. Pohlschroder M, Murphy C, Beckwith J. In vivo analyses of interactions between SecE and SecY, core components of the Escherichia coli protein translocation machinery. J Biol Chem. 1996;271:19908-14 pubmed
    We have carried out structure-function studies on the cytoplasmic membrane protein, SecE, a component of the Escherichia coli secretion machinery...
  76. Joly J, Wickner W. The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids. EMBO J. 1993;12:255-63 pubmed
    ..Strikingly, neither the SecE nor Band 1 subunits were cross-linked to any of the constructs and the membrane phospholipids were almost entirely ..
  77. Baars L, Wagner S, Wickström D, Klepsch M, Ytterberg A, van Wijk K, et al. Effects of SecE depletion on the inner and outer membrane proteomes of Escherichia coli. J Bacteriol. 2008;190:3505-25 pubmed publisher
    ..the role of the Sec translocon using subproteome analysis of cells depleted of the essential translocon component SecE. The steady-state proteomes and the proteome dynamics were evaluated using one- and two-dimensional gel analysis, ..
  78. Kaufmann A, Manting E, Veenendaal A, Driessen A, van der Does C. Cysteine-directed cross-linking demonstrates that helix 3 of SecE is close to helix 2 of SecY and helix 3 of a neighboring SecE. Biochemistry. 1999;38:9115-25 pubmed
    ..Unique cysteines were introduced into transmembrane segment (TMS) 2 of SecY and TMS 3 of SecE to probe possible sites of interaction between the integral membrane subunits...
  79. van Dalen A, van der Laan M, Driessen A, Killian J, de Kruijff B. Components required for membrane assembly of newly synthesized K+ channel KcsA. FEBS Lett. 2002;511:51-8 pubmed
    ..It is concluded that SecYEG and YidC are not required for the formation of tetrameric KcsA in vitro. ..
  80. Yi L, Celebi N, Chen M, Dalbey R. Sec/SRP requirements and energetics of membrane insertion of subunits a, b, and c of the Escherichia coli F1F0 ATP synthase. J Biol Chem. 2004;279:39260-7 pubmed
    ..Taken together, the in vivo data suggest that subunits a and b are inserted by the Sec/SRP pathway with the help of YidC, and subunit c is integrated into the membrane by the novel YidC pathway. ..
  81. Satoh Y, Mori H, Ito K. Nearest neighbor analysis of the SecYEG complex. 2. Identification of a SecY-SecE cytosolic interface. Biochemistry. 2003;42:7442-7 pubmed
    ..These cross-linkages were abolished by the Gly240 mutation in the SecY C4 region as well as by prlG alterations in SecE transmembrane segment 3, known to compromise SecY-SecE interaction...