secA

Summary

Gene Symbol: secA
Description: preprotein translocase subunit, ATPase
Alias: ECK0099, JW0096, azi, pea, prlD
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Nouwen N, Berrelkamp G, Driessen A. Charged amino acids in a preprotein inhibit SecA-dependent protein translocation. J Mol Biol. 2009;386:1000-10 pubmed publisher
    ..translocated polypeptide chain that blocks the translocation site and no longer activates preprotein-stimulated SecA ATPase activity...
  2. Brundage L, Hendrick J, Schiebel E, Driessen A, Wickner W. The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation. Cell. 1990;62:649-57 pubmed
    ..protein translocation in vitro using purified proteins (the precursor proOmpA, the chaperone SecB, and the ATPase SecA) in addition to isolated inner membrane vesicles...
  3. Suzuki H, Nishiyama K, Tokuda H. Coupled structure changes of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and delta secG::kan double mutant. Mol Microbiol. 1998;29:331-41 pubmed
    ..The mutant SecA (csSecA) was found to possess a single amino acid substitution in the precursor-binding region and was defective in ..
  4. Uchida K, Mori H, Mizushima S. Stepwise movement of preproteins in the process of translocation across the cytoplasmic membrane of Escherichia coli. J Biol Chem. 1995;270:30862-8 pubmed
    ..Treatment with these proteases resulted in complete digestion of SecA on everted membrane vesicles, whereas Sec proteins integrated into membranes were considerably resistant to the ..
  5. Karamanou S, Vrontou E, Sianidis G, Baud C, Roos T, Kuhn A, et al. A molecular switch in SecA protein couples ATP hydrolysis to protein translocation. Mol Microbiol. 1999;34:1133-45 pubmed
    b>SecA, the dimeric ATPase subunit of bacterial protein translocase, catalyses translocation during ATP-driven membrane cycling at SecYEG...
  6. Duong F, Wickner W. The SecDFyajC domain of preprotein translocase controls preprotein movement by regulating SecA membrane cycling. EMBO J. 1997;16:4871-9 pubmed
    ..hexameric complex of SecY, SecE, SecG, SecD, SecF and YajC (SecYEGDFyajC) and the peripheral ATPase SecA. The energy of ATP binding and hydrolysis promotes cycles of membrane insertion and deinsertion of SecA and ..
  7. Or E, Navon A, Rapoport T. Dissociation of the dimeric SecA ATPase during protein translocation across the bacterial membrane. EMBO J. 2002;21:4470-9 pubmed
    The ATPase SecA mediates post-translational translocation of precursor proteins through the SecYEG channel of the bacterial inner membrane...
  8. Chen Y, Pan X, Tang Y, Quan S, Tai P, Sui S. Full-length Escherichia coli SecA dimerizes in a closed conformation in solution as determined by cryo-electron microscopy. J Biol Chem. 2008;283:28783-7 pubmed publisher
    b>SecA is an obligatory component of the Escherichia coli general secretion pathway. However, the oligomeric structure of SecA and SecA conformational changes during translocation processes are still unclear...
  9. Papanikolau Y, Papadovasilaki M, Ravelli R, McCarthy A, Cusack S, Economou A, et al. Structure of dimeric SecA, the Escherichia coli preprotein translocase motor. J Mol Biol. 2007;366:1545-57 pubmed
    b>SecA is the preprotein translocase ATPase subunit and a superfamily 2 (SF2) RNA helicase...

More Information

Publications90

  1. Erlandson K, Miller S, Nam Y, Osborne A, Zimmer J, Rapoport T. A role for the two-helix finger of the SecA ATPase in protein translocation. Nature. 2008;455:984-7 pubmed publisher
    ..are generally translocated after completion of their synthesis by the interaction of the cytoplasmic ATPase SecA and a protein-conducting channel formed by the SecY complex...
  2. Gelis I, Bonvin A, Keramisanou D, Koukaki M, Gouridis G, Karamanou S, et al. Structural basis for signal-sequence recognition by the translocase motor SecA as determined by NMR. Cell. 2007;131:756-69 pubmed
    ..Here, we present the solution structure of a signal peptide bound to SecA, the 204 kDa ATPase motor of the Sec translocase...
  3. Koch H, Muller M. Dissecting the translocase and integrase functions of the Escherichia coli SecYEG translocon. J Cell Biol. 2000;150:689-94 pubmed
    Recent evidence suggests that in Escherichia coli, SecA/SecB and signal recognition particle (SRP) are constituents of two different pathways targeting secretory and inner membrane proteins to the SecYEG translocon of the plasma membrane...
  4. Baud C, Karamanou S, Sianidis G, Vrontou E, Politou A, Economou A. Allosteric communication between signal peptides and the SecA protein DEAD motor ATPase domain. J Biol Chem. 2002;277:13724-31 pubmed
    b>SecA, the preprotein translocase ATPase is built of an amino-terminal DEAD helicase motor domain bound to a regulatory C-domain. SecA recognizes mature and signal peptide preprotein regions...
  5. Woodbury R, Hardy S, Randall L. Complex behavior in solution of homodimeric SecA. Protein Sci. 2002;11:875-82 pubmed
    b>SecA, a homodimeric protein involved in protein export in Escherichia coli, exists in the cell both associated with the membrane translocation apparatus and free in the cytosol...
  6. Gold V, Robson A, Clarke A, Collinson I. Allosteric regulation of SecA: magnesium-mediated control of conformation and activity. J Biol Chem. 2007;282:17424-32 pubmed
    In bacteria, the SecA protein associates with a ubiquitous protein channel SecYEG where it drives the post-translational secretion of pre-proteins across the plasma membrane...
  7. Ding H, Mukerji I, Oliver D. Nucleotide and phospholipid-dependent control of PPXD and C-domain association for SecA ATPase. Biochemistry. 2003;42:13468-75 pubmed
    The SecA ATPase motor is a central component of the eubacterial protein translocation machinery...
  8. Tomkiewicz D, Nouwen N, van Leeuwen R, Tans S, Driessen A. SecA supports a constant rate of preprotein translocation. J Biol Chem. 2006;281:15709-13 pubmed
    ..membrane by the Sec system composed of a protein-conducting channel, SecYEG, and an ATP-dependent motor protein, SecA. After binding of the preprotein to SecYEG-bound SecA, cycles of ATP binding and hydrolysis by SecA are thought to ..
  9. Zhou J, Xu Z. The structural view of bacterial translocation-specific chaperone SecB: implications for function. Mol Microbiol. 2005;58:349-57 pubmed
    ..them to the translocon via its high-affinity binding to the ligand as well as to the membrane-embedded ATPase SecA. Recent advances in elucidating the structures of SecB have enabled the examination of protein function in the ..
  10. Muller M, Koch H, Beck K, Schafer U. Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane. Prog Nucleic Acid Res Mol Biol. 2001;66:107-57 pubmed
    ..In concert with SecB, SecA targets the precursors to SecY and energizes their translocation by its ATPase activity...
  11. Vrontou E, Karamanou S, Baud C, Sianidis G, Economou A. Global co-ordination of protein translocation by the SecA IRA1 switch. J Biol Chem. 2004;279:22490-7 pubmed
    b>SecA, the dimeric ATPase subunit of protein translocase, contains a DEAD helicase catalytic core that binds to a regulatory C-terminal domain...
  12. Robson A, Gold V, Hodson S, Clarke A, Collinson I. Energy transduction in protein transport and the ATP hydrolytic cycle of SecA. Proc Natl Acad Sci U S A. 2009;106:5111-6 pubmed publisher
    The motor protein SecA drives the transport of polypeptides through the ubiquitous protein channel SecYEG...
  13. Chun S, Randall L. In vivo studies of the role of SecA during protein export in Escherichia coli. J Bacteriol. 1994;176:4197-203 pubmed
    b>SecA is found in Escherichia coli both tightly associated with the cytoplasmic membrane where it functions as a translocation ATPase during protein export and free in the cytosol (R. J. Cabelli, K. M. Dolan, L. Qian, and D. B. Oliver, J...
  14. Wang H, Chen Y, Yang H, Chen X, Duan M, Tai P, et al. Ring-like pore structures of SecA: implication for bacterial protein-conducting channels. Proc Natl Acad Sci U S A. 2003;100:4221-6 pubmed
    b>SecA, an essential component of the general protein secretion pathway of bacteria, is present in Escherichia coli as soluble and membrane-integral forms...
  15. Randall L, Crane J, Lilly A, Liu G, Mao C, Patel C, et al. Asymmetric binding between SecA and SecB two symmetric proteins: implications for function in export. J Mol Biol. 2005;348:479-89 pubmed
    ..SecB also demonstrates specific recognition of, and binding to, SecA. SecB with the precursor tightly bound enters an export-active complex with SecA and must pass the ligand to SecA ..
  16. Robson A, Booth A, Gold V, Clarke A, Collinson I. A large conformational change couples the ATP binding site of SecA to the SecY protein channel. J Mol Biol. 2007;374:965-76 pubmed
    In bacteria, the SecYEG protein translocation complex employs the cytosolic ATPase SecA to couple the energy of ATP binding and hydrolysis to the mechanical force required to push polypeptides through the membrane...
  17. Osborne A, Rapoport T. Protein translocation is mediated by oligomers of the SecY complex with one SecY copy forming the channel. Cell. 2007;129:97-110 pubmed
    Many proteins are translocated across the bacterial plasma membrane by the interplay of the cytoplasmic ATPase SecA with a protein-conducting channel, formed from the evolutionarily conserved heterotrimeric SecY complex...
  18. Scotti P, Valent Q, Manting E, Urbanus M, Driessen A, Oudega B, et al. SecA is not required for signal recognition particle-mediated targeting and initial membrane insertion of a nascent inner membrane protein. J Biol Chem. 1999;274:29883-8 pubmed
    ..by its receptor, FtsY, mediates the transfer to a common translocation site in the inner membrane that contains SecA, SecG, and SecY...
  19. Douville K, Price A, Eichler J, Economou A, Wickner W. SecYEG and SecA are the stoichiometric components of preprotein translocase. J Biol Chem. 1995;270:20106-11 pubmed
    ..the Escherichia coli plasma membrane is catalyzed by preprotein translocase, comprised of the peripherally bound SecA subunit and an integrally bound heterotrimeric domain consisting of the SecY, SecE, and SecG subunits...
  20. Gouridis G, Karamanou S, Gelis I, Kalodimos C, Economou A. Signal peptides are allosteric activators of the protein translocase. Nature. 2009;462:363-7 pubmed publisher
    ..The main bacterial translocase comprises the SecYEG protein-conducting channel and the peripheral ATPase motor SecA. Most proteins destined for the periplasm and beyond are exported post-translationally by SecA...
  21. van Voorst F, Vereyken I, De Kruijff B. The high affinity ATP binding site modulates the SecA-precursor interaction. FEBS Lett. 2000;486:57-62 pubmed
    b>SecA is the central component of the protein-translocation machinery of Escherichia coli...
  22. Sun C, Rusch S, Kim J, Kendall D. Chloroplast SecA and Escherichia coli SecA have distinct lipid and signal peptide preferences. J Bacteriol. 2007;189:1171-5 pubmed
    Like prokaryotic Sec-dependent protein transport, chloroplasts utilize SecA. However, we observe distinctive requirements for the stimulation of chloroplast SecA ATPase activity; it is optimally stimulated in the presence of galactolipid ..
  23. Schmidt M, Brosh R, Oliver D. Escherichia coli SecA helicase activity is not required in vivo for efficient protein translocation or autogenous regulation. J Biol Chem. 2001;276:37076-85 pubmed
    b>SecA is an essential ATP-driven motor protein that binds to preproteins and the translocon to promote protein translocation across the eubacterial plasma membrane...
  24. Tziatzios C, Schubert D, Lotz M, Gundogan D, Betz H, Schagger H, et al. The bacterial protein-translocation complex: SecYEG dimers associate with one or two SecA molecules. J Mol Biol. 2004;340:513-24 pubmed
    ..The core complex SecYEG forms the protein channel and engages either ribosomes or the ATPase SecA, which drive translocation of unfolded polypeptide chains through the complex and into the periplasmic space...
  25. Bieker K, Silhavy T. The genetics of protein secretion in E. coli. Trends Genet. 1990;6:329-34 pubmed
    ..The picture emerging provides a useful paradigm for similar pathways in other organisms. ..
  26. Tang Y, Pan X, Tai P, Sui S. Electron microscopic visualization of asymmetric precursor translocation intermediates: SecA functions as a dimer. Sci China Life Sci. 2010;53:1049-56 pubmed publisher
    b>SecA, the ATPase of Sec translocase, mediates the post-translational translocation of preprotein through the protein-conducting channel SecYEG in the bacterial inner membrane...
  27. Kuruma Y, Nishiyama K, Shimizu Y, Muller M, Ueda T. Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins. Biotechnol Prog. 2005;21:1243-51 pubmed
    ..efficient membrane translocation of a presecretory protein (pOmpA) was obtained simply by the addition of only SecA and SecB. Proteinase K digestion clearly showed the successful translocation of pOmpA inside the vesicles...
  28. Eichler J, Brunner J, Wickner W. The protease-protected 30 kDa domain of SecA is largely inaccessible to the membrane lipid phase. EMBO J. 1997;16:2188-96 pubmed
    b>SecA binds to the inner membrane of Escherichia coli through low affinity lipid interactions or with high affinity at SecYEG, the integral domain of preprotein translocase...
  29. Yuan J, Zweers J, van Dijl J, Dalbey R. Protein transport across and into cell membranes in bacteria and archaea. Cell Mol Life Sci. 2010;67:179-99 pubmed publisher
    ..In this review, we will describe the different systems employed to translocate and insert proteins across or into the cytoplasmic membrane of archaea and bacteria. ..
  30. Manting E, Driessen A. Escherichia coli translocase: the unravelling of a molecular machine. Mol Microbiol. 2000;37:226-38 pubmed
    ..functional enzyme complex consists of the SecYEG integral membrane protein complex and a peripherally bound ATPase, SecA. Under translocation conditions, four SecYEG heterotrimers assemble into one large protein complex, forming a ..
  31. Cooper D, Smith V, Crane J, Roth H, Lilly A, Randall L. SecA, the motor of the secretion machine, binds diverse partners on one interactive surface. J Mol Biol. 2008;382:74-87 pubmed publisher
    ..and chloroplasts, the energy for the mechanical work of moving polypeptides through that channel is provided by SecA, a regulated ATPase...
  32. Yu D, Wowor A, Cole J, Kendall D. Defining the Escherichia coli SecA dimer interface residues through in vivo site-specific photo-cross-linking. J Bacteriol. 2013;195:2817-25 pubmed publisher
    The motor protein SecA is a core component of the bacterial general secretory (Sec) pathway and is essential for cell viability...
  33. Marrichi M, Camacho L, Russell D, DeLisa M. Genetic toggling of alkaline phosphatase folding reveals signal peptides for all major modes of transport across the inner membrane of bacteria. J Biol Chem. 2008;283:35223-35 pubmed publisher
  34. Rajapandi T, Oliver D. Integration of SecA protein into the Escherichia coli inner membrane is regulated by its amino-terminal ATP-binding domain. Mol Microbiol. 1996;20:43-51 pubmed
    b>SecA protein, the ATPase promoting translocation of proteins across the Escherichia coil inner membrane, contains two ATP-binding domains that differ greatly in their affinity for bound nucleotide...
  35. Scotti P, Urbanus M, Brunner J, de Gier J, von Heijne G, van der Does C, et al. YidC, the Escherichia coli homologue of mitochondrial Oxa1p, is a component of the Sec translocase. EMBO J. 2000;19:542-9 pubmed
    ..Previous work has also identified the peripherally associated SecA protein as well as the SecD, SecF and YajC inner membrane proteins as components of the translocase...
  36. Bowers C, Lau F, Silhavy T. Secretion of LamB-LacZ by the signal recognition particle pathway of Escherichia coli. J Bacteriol. 2003;185:5697-705 pubmed
    ..the absence of the ribosome-associated chaperone, trigger factor (Tig), LamB-LacZ localized to the periplasm in a SecA-dependent, SRP-independent fashion...
  37. Manting E, Kaufmann A, van der Does C, Driessen A. A single amino acid substitution in SecY stabilizes the interaction with SecA. J Biol Chem. 1999;274:23868-74 pubmed
    ..It binds the peripheral ATPase SecA to form the translocase...
  38. Fekkes P, de Wit J, Boorsma A, Friesen R, Driessen A. Zinc stabilizes the SecB binding site of SecA. Biochemistry. 1999;38:5111-6 pubmed
    The molecular chaperone SecB targets preproteins to SecA at the translocation sites in the cytoplasmic membrane of Escherichia coli...
  39. Nakatogawa H, Murakami A, Ito K. Control of SecA and SecM translation by protein secretion. Curr Opin Microbiol. 2004;7:145-50 pubmed
    b>SecA, the protein translocation ATPase of E. coli is subject to secretion-defect-response control. SecM (secretion monitor) encoded by the 5' region of the secM-secA mRNA is involved in this regulation...
  40. Wang H, Na B, Yang H, Tai P. Additional in vitro and in vivo evidence for SecA functioning as dimers in the membrane: dissociation into monomers is not essential for protein translocation in Escherichia coli. J Bacteriol. 2008;190:1413-8 pubmed
    b>SecA is an essential component in the Sec-dependent protein translocation pathway and, together with ATP, provides the driving force for the transport of secretory proteins across the cytoplasmic membrane of Escherichia coli...
  41. van der Wolk J, de Wit J, Driessen A. The catalytic cycle of the escherichia coli SecA ATPase comprises two distinct preprotein translocation events. EMBO J. 1997;16:7297-304 pubmed
    b>SecA is the ATP-dependent force generator in the Escherichia coli precursor protein translocation cascade, and is bound at the membrane surface to the integral membrane domain of the preprotein translocase...
  42. Benach J, Chou Y, Fak J, Itkin A, Nicolae D, Smith P, et al. Phospholipid-induced monomerization and signal-peptide-induced oligomerization of SecA. J Biol Chem. 2003;278:3628-38 pubmed
    The SecA ATPase drives the processive translocation of the N terminus of secreted proteins through the cytoplasmic membrane in eubacteria via cycles of binding and release from the SecYEG translocon coupled to ATP turnover...
  43. Lill R, Cunningham K, Brundage L, Ito K, Oliver D, Wickner W. SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli. EMBO J. 1989;8:961-6 pubmed
    ..This translocation ATPase requires inner membrane vesicles, SecA protein and translocation-competent proOmpA...
  44. Ernst F, Hoffschulte H, Thome Kromer B, Swidersky U, Werner P, Muller M. Precursor-specific requirements for SecA, SecB, and delta muH+ during protein export of Escherichia coli. J Biol Chem. 1994;269:12840-5 pubmed
    ..periplasmic protein Skp of Escherichia coli and demonstrate a precursor-specific dependence on the export factors SecA, SecB, and the proton-motive force (delta mu H+)...
  45. Houben E, Scotti P, Valent Q, Brunner J, de Gier J, Oudega B, et al. Nascent Lep inserts into the Escherichia coli inner membrane in the vicinity of YidC, SecY and SecA. FEBS Lett. 2000;476:229-33 pubmed
    ..experiments showed that the hydrophilic region P1 of nascent membrane-inserted Lep 100-mer was in the vicinity of SecA and SecY, whereas the first transmembrane domain H1 was in the vicinity of YidC...
  46. Den Blaauwen T, Terpetschnig E, Lakowicz J, Driessen A. Interaction of SecB with soluble SecA. FEBS Lett. 1997;416:35-8 pubmed
    ..by preventing the premature folding of the preprotein in the cytosol, and targeting it to the peripheral subunit SecA of the translocase at the cytoplasmic membrane...
  47. Maki J, Krishnan B, Gierasch L. Using a low denaturant model to explore the conformational features of translocation-active SecA. Biochemistry. 2012;51:1369-79 pubmed publisher
    The SecA molecular nanomachine in bacteria uses energy from ATP hydrolysis to drive post-translational secretion of preproteins through the SecYEG translocon...
  48. Bieker K, Phillips G, Silhavy T. The sec and prl genes of Escherichia coli. J Bioenerg Biomembr. 1990;22:291-310 pubmed
    ..Subsequent characterization reveals that in at least three cases, prlA/secY, prlD/secA, and prlG/secE, both types of mutations are found within the same structural gene...
  49. Khalili Yazdi A, Namjoshi S, Hackett J, Ghonaim N, Shilton B. Characterization of a polypeptide-binding site in the DEAD Motor of the SecA ATPase. FEBS Lett. 2017;591:3378-3390 pubmed publisher
    ..b>SecA-N95, SecA-N68, and SecA-DM (which consists of only the DEAD Motor domains NBD1 and NBD2) bound to the immobilized ..
  50. Kosic N, Sugai M, Fan C, Wu H. Processing of lipid-modified prolipoprotein requires energy and sec gene products in vivo. J Bacteriol. 1993;175:6113-7 pubmed
    ..e., secA, secE, secY, secD, and secF, and by metabolic poisons which affect proton motive force (PMF)...
  51. de Keyzer J, van der Sluis E, Spelbrink R, Nijstad N, de Kruijff B, Nouwen N, et al. Covalently dimerized SecA is functional in protein translocation. J Biol Chem. 2005;280:35255-60 pubmed
    The ATPase SecA provides the driving force for the transport of secretory proteins across the cytoplasmic membrane of Escherichia coli...
  52. Bankaitis V, Bassford P. Proper interaction between at least two components is required for efficient export of proteins to the Escherichia coli cell envelope. J Bacteriol. 1985;161:169-78 pubmed
    ..coli linkage map, and that 1 maps at a new locus, prlD, at 2.5 min on the linkage map...
  53. van Bloois E, ten Hagen Jongman C, Luirink J. Flexibility in targeting and insertion during bacterial membrane protein biogenesis. Biochem Biophys Res Commun. 2007;362:727-33 pubmed
    ..particle (SRP), the Sec-translocon and YidC with translocation of (large) periplasmic domains energized by SecA and the proton motive force (pmf)...
  54. Nithianantham S, Shilton B. Analysis of the isolated SecA DEAD motor suggests a mechanism for chemical-mechanical coupling. J Mol Biol. 2008;383:380-9 pubmed publisher
    The preprotein cross-linking domain and C-terminal domains of Escherichia coli SecA were removed to create a minimal DEAD motor, SecA-DM. SecA-DM hydrolyzes ATP and has the same affinity for ADP as full-length SecA...
  55. Papanikou E, Karamanou S, Baud C, Frank M, Sianidis G, Keramisanou D, et al. Identification of the preprotein binding domain of SecA. J Biol Chem. 2005;280:43209-17 pubmed
    b>SecA, the preprotein translocase ATPase, has a helicase DEAD motor. To catalyze protein translocation, SecA possesses two additional flexible domains absent from other helicases...
  56. Natale P, Swaving J, van der Does C, de Keyzer J, Driessen A. Binding of SecA to the SecYEG complex accelerates the rate of nucleotide exchange on SecA. J Biol Chem. 2004;279:13769-77 pubmed
    SecYEG translocase mediates the transport of preproteins across the inner membrane of Escherichia coli. SecA binds the membrane-embedded SecYEG protein-conducting channel with high affinity and then drives the stepwise translocation of ..
  57. van der Does C, Manting E, Kaufmann A, Lutz M, Driessen A. Interaction between SecA and SecYEG in micellar solution and formation of the membrane-inserted state. Biochemistry. 1998;37:201-10 pubmed
    Preprotein translocation in Escherichia coli is mediated by the translocase with SecA as peripheral ATPase and SecY, SecE, and SecG as membrane domain...
  58. Sugai R, Takemae K, Tokuda H, Nishiyama K. Topology inversion of SecG is essential for cytosolic SecA-dependent stimulation of protein translocation. J Biol Chem. 2007;282:29540-8 pubmed
    ..In the absence of externally added SecA, SecG-PhoA IMVs were as active in protein translocation as SecG(+) IMVs per SecA...
  59. Murakami A, Nakatogawa H, Ito K. Translation arrest of SecM is essential for the basal and regulated expression of SecA. Proc Natl Acad Sci U S A. 2004;101:12330-5 pubmed
    ..Arrest-alleviating mutations in the arrest sequence reduced the expression of secA, a downstream gene on the same mRNA...
  60. Veenendaal A, van der Does C, Driessen A. The protein-conducting channel SecYEG. Biochim Biophys Acta. 2004;1694:81-95 pubmed
    ..consists of a membrane embedded protein-conducting channel and a peripherally associated motor domain, the ATPase SecA. The channel is formed by SecYEG, a multimeric protein complex that assembles into oligomeric forms...
  61. Schmidt M, Dolan K, Oliver D. Regulation of Escherichia coli secA mRNA translation by a secretion-responsive element. J Bacteriol. 1991;173:6605-11 pubmed
    The Escherichia coli secA gene, whose translation is responsive to the proficiency of protein export within the cell, is the second gene in a three-gene operon and is flanked by gene X and mutT...
  62. Prabudiansyah I, Kusters I, Caforio A, Driessen A. Characterization of the annular lipid shell of the Sec translocon. Biochim Biophys Acta. 2015;1848:2050-6 pubmed publisher
    ..minimal form consists of a membrane-embedded protein-conducting pore SecYEG that interacts with the motor protein SecA to mediate the translocation of secretory proteins...
  63. van der Sluis E, Driessen A. Stepwise evolution of the Sec machinery in Proteobacteria. Trends Microbiol. 2006;14:105-8 pubmed
    ..The Sec machinery of Escherichia coli and other Enterobacteriales represents the end product of this stepwise evolution. ..
  64. Mitchell C, Oliver D. Two distinct ATP-binding domains are needed to promote protein export by Escherichia coli SecA ATPase. Mol Microbiol. 1993;10:483-97 pubmed
    Six putative ATP-binding motifs of SecA protein were altered by oligonucleotide-directed mutagenesis to try to define the ATP-binding regions of this multifunctional protein...
  65. Snyders S, Ramamurthy V, Oliver D. Identification of a region of interaction between Escherichia coli SecA and SecY proteins. J Biol Chem. 1997;272:11302-6 pubmed
    b>SecA ATPase promotes Escherichia coli protein translocation by its association with the preprotein or preprotein-SecB complex, anionic phospholipids, and the other core component of translocase, integral membrane protein SecYEG...
  66. de Gier J, Luirink J. Biogenesis of inner membrane proteins in Escherichia coli. Mol Microbiol. 2001;40:314-22 pubmed
    ..Here, we review and discuss recent advances in our understanding of the biogenesis of inner membrane proteins in E. coli. ..
  67. Koonin E, Gorbalenya A. Autogenous translation regulation by Escherichia coli ATPase SecA may be mediated by an intrinsic RNA helicase activity of this protein. FEBS Lett. 1992;298:6-8 pubmed
    ..motifs typical of the helicase superfamily II have been identified in the sequences of Escherichia coli protein SecA, an ATPase mediating protein translocation across the inner membrane of the bacterium, and its Bacillus subtilis ..
  68. Driessen A, Wickner W. Solubilization and functional reconstitution of the protein-translocation enzymes of Escherichia coli. Proc Natl Acad Sci U S A. 1990;87:3107-11 pubmed
    ..Translocation required ATP, SecY, and SecA and was stimulated by a proton-motive force...
  69. Hsieh Y, Zhang H, Jin J, Dai C, Jiang C, Wang B, et al. Biphasic actions of SecA inhibitors on Prl/Sec suppressors: Possible physiological roles of SecA-only channels. Biochem Biophys Res Commun. 2017;482:296-300 pubmed publisher
    b>SecA is an essential component in the bacterial Sec-dependent protein translocation process...
  70. du Plessis D, Nouwen N, Driessen A. Subunit a of cytochrome o oxidase requires both YidC and SecYEG for membrane insertion. J Biol Chem. 2006;281:12248-52 pubmed
    ..Efficient insertion of in vitro synthesized pre-CyoA into proteoliposomes requires YidC, SecYEG, and SecA and occurs independently of the proton motive force...
  71. Suo Y, Hardy S, Randall L. The basis of asymmetry in the SecA:SecB complex. J Mol Biol. 2015;427:887-900 pubmed publisher
    ..coli, SecB, a homotetramer structurally organized as a dimer of dimers, forms a complex with two protomers of SecA, which is the ATPase that provides energy to transfer a precursor polypeptide through the membrane via the SecYEG ..
  72. McMurry J, Kendall D. An artificial transmembrane segment directs SecA, SecB, and electrochemical potential-dependent translocation of a long amino-terminal tail. J Biol Chem. 1999;274:6776-82 pubmed
    ..transmembrane segments that demonstrate that translocation of a long N-tail across the membrane is dependent upon SecA, SecB, and the electrochemical potential in the absence of a signal peptide...
  73. Schmidt M, Ding H, Ramamurthy V, Mukerji I, Oliver D. Nucleotide binding activity of SecA homodimer is conformationally regulated by temperature and altered by prlD and azi mutations. J Biol Chem. 2000;275:15440-8 pubmed
    b>SecA ATPase is critical for protein translocation across the Escherichia coli inner membrane. To understand this activity further, the high affinity nucleotide binding activity of SecA was characterized...
  74. Nakatogawa H, Mori H, Matsumoto G, Ito K. Characterization of a mutant form of SecA that alleviates a SecY defect at low temperature and shows a synthetic defect with SecY alteration at high temperature. J Biochem. 2000;127:1071-9 pubmed
    ..cold-sensitive for protein export, with an in vitro defect in supporting ATP- and preprotein-dependent insertion of SecA into the membrane...
  75. Mori H, Ito K. The long alpha-helix of SecA is important for the ATPase coupling of translocation. J Biol Chem. 2006;281:36249-56 pubmed
    b>SecA contains two ATPase folds (NBF1 and NBF2) and other interaction/regulatory domains, all of which are connected by a long helical scaffold domain (HSD) running along the molecule...
  76. Liang F, Bageshwar U, Musser S. Bacterial Sec protein transport is rate-limited by precursor length: a single turnover study. Mol Biol Cell. 2009;20:4256-66 pubmed publisher
    ..driven by a series of rate-limiting, discrete translocation steps that result from conformational cycling of the SecA ATPase...
  77. Sanders C, Wethkamp N, Lill H. Transport of cytochrome c derivatives by the bacterial Tat protein translocation system. Mol Microbiol. 2001;41:241-6 pubmed
    ..We conclude that, in addition to the nature of the specific signal peptide, the folding state of a particular protein also governs its acceptance by a given transport system. ..
  78. Dapic V, Oliver D. Distinct membrane binding properties of N- and C-terminal domains of Escherichia coli SecA ATPase. J Biol Chem. 2000;275:25000-7 pubmed
    b>SecA is a motor protein that drives protein translocation at the Escherichia coli translocon. SecA membrane binding has been shown to occur with high affinity at SecYE and low affinity at anionic phospholipids...
  79. Lin B, Gierasch L, Jiang C, Tai P. Electrophysiological studies in Xenopus oocytes for the opening of Escherichia coli SecA-dependent protein-conducting channels. J Membr Biol. 2006;214:103-13 pubmed
    ..Here, we report a novel, sensitive method that detects the opening of the SecA-dependent protein-conducting channels at the nanogram level. E...
  80. Zhang Q, Li Y, Olson R, Mukerji I, Oliver D. Conserved SecA Signal Peptide-Binding Site Revealed by Engineered Protein Chimeras and Förster Resonance Energy Transfer. Biochemistry. 2016;55:1291-300 pubmed publisher
    ..peptides are critical for the initiation of protein transport in bacteria by virtue of their recognition by the SecA ATPase motor protein followed by their transfer to the lateral gate region of the SecYEG protein-conducting channel ..