sapD

Summary

Gene Symbol: sapD
Description: antimicrobial peptide ABC transporter ATPase
Alias: ECK1286, JW1284, trkE
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Sugiyama Y, Nakamura A, Matsumoto M, Kanbe A, Sakanaka M, Higashi K, et al. A Novel Putrescine Exporter SapBCDF of Escherichia coli. J Biol Chem. 2016;291:26343-26351 pubmed
    ..coli ?sapBCDF strain did not affect resistance to antimicrobial peptide LL-37. These results strongly suggest that the natural function of the SapBCDF proteins is the export of putrescine. ..
  2. Dosch D, Helmer G, Sutton S, Salvacion F, Epstein W. Genetic analysis of potassium transport loci in Escherichia coli: evidence for three constitutive systems mediating uptake potassium. J Bacteriol. 1991;173:687-96 pubmed
    ..Bakker, J. Biol. Chem. 264:16403-16410, 1989). The TrkH system is also dependent on the products of the trkH and trkE genes, while the TrkG system is also dependent on the product of the trkG gene and partially dependent on the ..
  3. Trchounian A, Bagramyan K, Vassilian A, Poladian A. Relationship between formate hydrogen lyase and proton-potassium pump under heterolactic fermentation in Escherichia coli: functional multienzyme associations in the cell membrane. Membr Cell Biol. 2000;13:511-26 pubmed
    ..The number of SH groups decreased in the presence of DCC. These results indicate a significance of the thiol groups for the function of the proposed association. ..
  4. Bagramyan K, Martirosov S. Formation of an ion transport supercomplex in Escherichia coli. An experimental model of direct transduction of energy. FEBS Lett. 1989;246:149-52 pubmed
    ..Within this supercomplex direct transduction of energy is accomplished via oxidation of 2 SH to S-S. ..
  5. Trchunian A, Ogandzhanian E, Martirosov S. [N,N'-dicyclohexylcarbodiimide-sensitive K+-dependent ATPase activity in Escherichia coli]. Nauchnye Doki Vyss Shkoly Biol Nauki. 1989;:90-5 pubmed
  6. Trchounian A, Vassilian A. Relationship between the F0F1-ATPase and the K(+)-transport system within the membrane of anaerobically grown Escherichia coli. N,N'-dicyclohexylcarbodiimide-sensitive ATPase activity in mutants with defects in K(+)-transport. J Bioenerg Biomembr. 1994;26:563-71 pubmed
    ..This effect was observed only in parent and in the trkG, but not in the trkA, trkE, or trkH mutants...
  7. Stewart L, Bakker E, Booth I. Energy coupling to K+ uptake via the Trk system in Escherichia coli: the role of ATP. J Gen Microbiol. 1985;131:77-85 pubmed
    ..Using inhibitors and unc mutants we show that Trk is not an ATPase but may be regulated by ATP. Possible mechanisms of energy coupling to Trk are discussed. ..
  8. Epstein W, Kim B. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971;108:639-44 pubmed
    ..The trk loci are widely scattered over the E. coli chromosome; none of them is very near any other trk locus or near the kdp genes. ..
  9. Trchunian A, Ogandzhanian E, Mironova G, Martirosov S. [K+-dependent enzyme activity of H+-ATPase in the E. coli membrane]. Biofizika. 1991;36:102-4 pubmed
    ..coli depended on K+ activity. F1F0 include two additional proteins which correspond to the Trk system. The data improve the possibility to form supercomplex (F1F0-Trk) functioning as the H(+)-K(+)-pump. ..

Scientific Experts

More Information

Publications22

  1. Bossemeyer D, Borchard A, Dosch D, Helmer G, Epstein W, Booth I, et al. K+-transport protein TrkA of Escherichia coli is a peripheral membrane protein that requires other trk gene products for attachment to the cytoplasmic membrane. J Biol Chem. 1989;264:16403-10 pubmed
    ..Mutations in the three other genes implicated in Trk activity, trkE, trkG, and trkH, reduced or abolished the binding of TrkA to the membrane...
  2. Groisman E, Parra Lopez C, Salcedo M, Lipps C, Heffron F. Resistance to host antimicrobial peptides is necessary for Salmonella virulence. Proc Natl Acad Sci U S A. 1992;89:11939-43 pubmed
    ..Our data indicate that the ability to resist the killing effect of host antimicrobial peptides is a virulence property and that several resistance mechanisms operate in S. typhimurium. ..
  3. Parra Lopez C, Lin R, Aspedon A, Groisman E. A Salmonella protein that is required for resistance to antimicrobial peptides and transport of potassium. EMBO J. 1994;13:3964-72 pubmed
    ..SapG may modulate the activities of SapABCDF and SapJ to mediate the transport of peptides and potassium. ..
  4. Epstein W, Buurman E, McLaggan D, Naprstek J. Multiple mechanisms, roles and controls of K+ transport in Escherichia coli. Biochem Soc Trans. 1993;21:1006-10 pubmed
  5. Parra Lopez C, Baer M, Groisman E. Molecular genetic analysis of a locus required for resistance to antimicrobial peptides in Salmonella typhimurium. EMBO J. 1993;12:4053-62 pubmed
    ..from the wild-type plasmid conferring resistance to protamine contained five open reading frames: sapA, sapB, sapC, sapD and sapF, organized in an operon structure and transcribed as a 5.3 kb mRNA...
  6. Schlosser A, Hamann A, Bossemeyer D, Schneider E, Bakker E. NAD+ binding to the Escherichia coli K(+)-uptake protein TrkA and sequence similarity between TrkA and domains of a family of dehydrogenases suggest a role for NAD+ in bacterial transport. Mol Microbiol. 1993;9:533-43 pubmed
    ..Strong u.v. illumination at 252 nm led to cross-linking of NAD+ or NADH, but not of ATP to the isolated TrkA protein...
  7. Trchounian A, Kobayashi H. K+ uptake by fermenting Escherichia coli cells: pH dependent mode of the TrkA system operating. Biosci Rep. 2000;20:277-88 pubmed
    ..0 than that for cells grown at pH 7.3; (4) the DCCD-inhibited ATPase activity of membrane vesicles from bacteria grown at pH 9.0 was reduced a little in the presence of 100 mM K+, but stimulated more than 2.4-fold at pH 7.3. ..
  8. Trchounian A. Ion exchange in facultative anaerobes: does a proton-potassium pump exist in anaerobic Escherichia Coli?. Anaerobe. 1997;3:355-71 pubmed
  9. Schl sser A, Meldorf M, Stumpe S, Bakker E, Epstein W. TrkH and its homolog, TrkG, determine the specificity and kinetics of cation transport by the Trk system of Escherichia coli. J Bacteriol. 1995;177:1908-10 pubmed
    ..The kinetics of transport in strains with wild-type alleles at trkG and at trkH suggest that both products participate in transport...
  10. Stumpe -, Bakker -. Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch Microbiol. 1997;167:126-36 pubmed
    ..Cells that cannot take up K+ rapidly remain metabolically compromised to such an extent that extracytoplasmic protease activity is not induced, leading to a prolonged susceptibility of the cells to the toxic peptide. ..
  11. Trchunian A, Durgar ian S, Ogandzhanian E, Ter Nikogosian V, Vardanian A. [The capacity of anaerobically grown bacteria to exchange the 2H+ of the cell for the K+ of the medium and to maintain a high K+ distribution between cell and medium]. Nauchnye Doki Vyss Shkoly Biol Nauki. 1986;:82-8 pubmed
  12. Stumpe S, Bakker E. Requirement of a large K+-uptake capacity and of extracytoplasmic protease activity for protamine resistance of Escherichia coli. Arch Microbiol. 1997;167:126-36 pubmed
    ..Cells that cannot take up K+ rapidly remain metabolically compromised to such an extent that extracytoplasmic protease activity is not induced, leading to a prolonged susceptibility of the cells to the toxic peptide. ..
  13. Harms C, Domoto Y, Celik C, Rahe E, Stumpe S, Schmid R, et al. Identification of the ABC protein SapD as the subunit that confers ATP dependence to the K+-uptake systems Trk(H) and Trk(G) from Escherichia coli K-12. Microbiology. 2001;147:2991-3003 pubmed
    ..All five plasmid-encoded E. coli Sap proteins were made in E. coli mini-cells. The presence of the ATP-binding SapD protein from either E. coli or V...