Gene Symbol: rsxE
Description: SoxR iron-sulfur cluster reduction factor component; electron transport inner membrane NADH-quinone reductase
Alias: ECK1628, JW1624, rnfE, ydgQ
Species: Escherichia coli str. K-12 substr. MG1655
Kumagai H, Fujiwara T, Matsubara H, Saeki K. Membrane localization, topology, and mutual stabilization of the rnfABC gene products in Rhodobacter capsulatus and implications for a new family of energy-coupling NADH oxidoreductases. Biochemistry. 1997;36:5509-21 pubmed publisher
..Rhodobacter capsulatus are unique nitrogen fixation genes that encode potential membrane proteins (RnfA, RnfD, and RnfE) and potential iron-sulfur proteins (RnfB and RnfC)...
Saaf A, Johansson M, Wallin E, von Heijne G. Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues. Proc Natl Acad Sci U S A. 1999;96:8540-4 pubmed
..This example of divergent evolution of membrane protein topology suggests that a complete inversion of membrane topology is possible with relatively few mutational changes even for proteins with multiple transmembrane segments. ..
Rapp M, Granseth E, Seppälä S, von Heijne G. Identification and evolution of dual-topology membrane proteins. Nat Struct Mol Biol. 2006;13:112-6 pubmed
..Our results provide strong support for the existence of dual-topology proteins and shed new light on the evolution of membrane-protein topology and structure. ..
Jouanneau Y, Jeong H, Hugo N, Meyer C, Willison J. Overexpression in Escherichia coli of the rnf genes from Rhodobacter capsulatus--characterization of two membrane-bound iron-sulfur proteins. Eur J Biochem. 1998;251:54-64 pubmed
..coli as His-tagged polypeptides, whereas the products of rnfA, rnfD and rnfE, predicted to be transmembrane proteins, could not be stably maintained in E. coli...
Gifford C, Wallace S. The genes encoding endonuclease VIII and endonuclease III in Escherichia coli are transcribed as the terminal genes in operons. Nucleic Acids Res. 2000;28:762-9 pubmed
..The six open reading frames immediately upstream of nth show homology to the genes rnfA, rnfB, rnfC, rnfD, rnfG and rnfE from Rhodobacter capsulatis. The rnf genes are required for nitrogen fixation in R...
Koo M, Lee J, Rah S, Yeo W, Lee J, Lee K, et al
. A reducing system of the superoxide sensor SoxR in Escherichia coli. EMBO J. 2003;22:2614-22 pubmed publisher
..Electron paramagnetic resonance analysis of SoxR and measurement of re-reduction kinetics support the proposal that rsx and rseC gene products constitute a reducing system for SoxR...
Twiss E, Coros A, Tavakoli N, Derbyshire K. Transposition is modulated by a diverse set of host factors in Escherichia coli and is stimulated by nutritional stress. Mol Microbiol. 2005;57:1593-607 pubmed
..In summary, this work identifies a collection of proteins that allow the host to modulate transposition in response to cell stress. ..