Gene Symbol: rsuA
Description: 16S rRNA pseudouridine(516) synthase
Alias: ECK2177, JW2171, yejD
Species: Escherichia coli str. K-12 substr. MG1655
Conrad J, Niu L, Rudd K, Lane B, Ofengand J. 16S ribosomal RNA pseudouridine synthase RsuA of Escherichia coli: deletion, mutation of the conserved Asp102 residue, and sequence comparison among all other pseudouridine synthases. RNA. 1999;5:751-63 pubmed
The gene for RsuA, the pseudouridine synthase that converts U516 to pseudouridine in 16S ribosomal RNA of Escherichia coli, has been deleted in strains MG1655 and BL21/DE3...
Wrzesinski J, Bakin A, Nurse K, Lane B, Ofengand J. Purification, cloning, and properties of the 16S RNA pseudouridine 516 synthase from Escherichia coli. Biochemistry. 1995;34:8904-13 pubmed
..subtilis has the equivalent of psi 516 in its small subunit rRNA. On the other hand, no common sequence motifs could be detected among this enzyme and the two tRNA psi synthases which have been cloned up to now. ..
Sivaraman J, Sauv V, Larocque R, Stura E, Schrag J, Cygler M, et al
. Structure of the 16S rRNA pseudouridine synthase RsuA bound to uracil and UMP. Nat Struct Biol. 2002;9:353-8 pubmed publisher
In Escherichia coli, the pseudouridine synthase RsuA catalyzes formation of pseudouridine (psi) at position 516 in 16S rRNA during assembly of the 30S ribosomal subunit...
Hamma T, Ferré D Amaré A. Pseudouridine synthases. Chem Biol. 2006;13:1125-35 pubmed
Matte A, Jia Z, Sunita S, Sivaraman J, Cygler M. Insights into the biology of Escherichia coli through structural proteomics. J Struct Funct Genomics. 2007;8:45-55 pubmed
..Together, these results illustrate the power of structural genomics to contribute to a deeper biological understanding of bacterial processes. ..
Rosenberg S. Life, death, differentiation, and the multicellularity of bacteria. PLoS Genet. 2009;5:e1000418 pubmed publisher
Hur S, Stroud R, Finer Moore J. Substrate recognition by RNA 5-methyluridine methyltransferases and pseudouridine synthases: a structural perspective. J Biol Chem. 2006;281:38969-73 pubmed
Amitai S, Kolodkin Gal I, Hananya Meltabashi M, Sacher A, Engelberg Kulka H. Escherichia coli MazF leads to the simultaneous selective synthesis of both "death proteins" and "survival proteins". PLoS Genet. 2009;5:e1000390 pubmed publisher