Gene Symbol: rstB
Description: sensory histidine kinase of RstAB two-component system
Alias: ECK1604, JW1601, uspT
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Mizuno T. Compilation of all genes encoding two-component phosphotransfer signal transducers in the genome of Escherichia coli. DNA Res. 1997;4:161-8 pubmed
    ..In addition, E. coli has five hybrid sensory kinases. The precise location of each ORF was mapped on a physical map of the entire E. coli genome. All of these ORFs were then compiled and annotated extensively. ..
  2. Minagawa S, Ogasawara H, Kato A, Yamamoto K, Eguchi Y, Oshima T, et al. Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli. J Bacteriol. 2003;185:3696-702 pubmed
    ..Thus, we concluded that the phoPQ, mgrB, mgtA, hemL, nagA, rstAB, slyB, vboR, and yrbL genes make up the Mg(2+) stimulon in E. coli...
  3. Hagiwara D, Yamashino T, Mizuno T. A Genome-wide view of the Escherichia coli BasS-BasR two-component system implicated in iron-responses. Biosci Biotechnol Biochem. 2004;68:1758-67 pubmed
    ..No physiological stimulus has yet been identified for each of the remaining 6 His-kinases (BasS, CreC, RstB, YfhK, YehU, and YpdA)...
  4. Yamamoto K, Hirao K, Oshima T, Aiba H, Utsumi R, Ishihama A. Functional characterization in vitro of all two-component signal transduction systems from Escherichia coli. J Biol Chem. 2005;280:1448-56 pubmed
    ..A total of 30 sensor HKs, including as yet uncharacterized putative HKs (BaeS, BasS, CreC, CusS, HydH, RstB, YedV, and YfhK), and a total of 34 RRs, including putative RRs (BaeR, BasR, CreB, CusR, HydG, RstA, YedW, YfhA, ..
  5. Roecklein B, Pelletier A, Kuempel P. The tus gene of Escherichia coli: autoregulation, analysis of flanking sequences and identification of a complementary system in Salmonella typhimurium. Res Microbiol. 1991;142:169-75 pubmed
    ..Sequence homology can be demonstrated by Southern hybridizations, and the systems also exhibit functional complementation: the Tus protein of S. typhimurium blocks DNA replication at the TerA site of E. coli. ..
  6. Roecklein B, Kuempel P. In vivo characterization of tus gene expression in Escherichia coli. Mol Microbiol. 1992;6:1655-61 pubmed
    ..We have also demonstrated that a minority of tus transcripts are initiated from an upstream region that contains two additional open reading frames. This readthrough transcription into tus is reduced in the presence of Tus protein. ..
  7. Eguchi Y, Okada T, Minagawa S, Oshima T, Mori H, Yamamoto K, et al. Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia coli. J Bacteriol. 2004;186:3006-14 pubmed
    ..resulted in enhanced expression of 13 PhoP/PhoQ-regulated genes, crcA, hemL, mgtA, ompT, phoP, phoQ, proP, rstA, rstB, slyB, ybjG, yrbL, and mgrB...
  8. Kefala G, Kwiatkowski W, Esquivies L, Maslennikov I, Choe S. Application of Mistic to improving the expression and membrane integration of histidine kinase receptors from Escherichia coli. J Struct Funct Genomics. 2007;8:167-72 pubmed
    ..Mistic fusion methods will likely be instrumental for high-level expression of other integral membrane proteins. ..
  9. Ashenberg O, Rozen Gagnon K, Laub M, Keating A. Determinants of homodimerization specificity in histidine kinases. J Mol Biol. 2011;413:222-35 pubmed publisher
    ..We investigated the molecular basis of dimerization specificity, focusing on the model histidine kinase EnvZ and RstB, its closest paralog in Escherichia coli...

More Information


  1. Hill T, Tecklenburg M, Pelletier A, Kuempel P. tus, the trans-acting gene required for termination of DNA replication in Escherichia coli, encodes a DNA-binding protein. Proc Natl Acad Sci U S A. 1989;86:1593-7 pubmed
    ..Insertion of a kanamycin-resistance gene in this open reading frame abolished tus activity. We also demonstrated that crude extracts of tus+ cells contain a protein which binds to the T2 terminator sequence. ..