Gene Symbol: rsgA
Description: ribosome small subunit-dependent GTPase A
Alias: ECK4157, JW4122, engC, yjeQ
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Daigle D, Rossi L, Berghuis A, Aravind L, Koonin E, Brown E. YjeQ, an essential, conserved, uncharacterized protein from Escherichia coli, is an unusual GTPase with circularly permuted G-motifs and marked burst kinetics. Biochemistry. 2002;41:11109-17 pubmed
    The Escherichia coli protein YjeQ represents a protein family whose members are broadly conserved in bacteria and have been shown to be indispensable to the growth of E. coli and Bacillus subtilis [Arigoni, F., et al. (1998) Nat...
  2. Daigle D, Brown E. Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro. J Bacteriol. 2004;186:1381-7 pubmed
    Escherichia coli YjeQ represents a conserved group of bacteria-specific nucleotide-binding proteins of unknown physiological function that have been shown to be essential to the growth of E. coli and Bacillus subtilis...
  3. Himeno H, Hanawa Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, et al. A novel GTPase activated by the small subunit of ribosome. Nucleic Acids Res. 2004;32:5303-9 pubmed
    The GTPase activity of Escherichia coli YjeQ, here named RsgA (ribosome small subunit-dependent GTPase A), has been shown to be significantly enhanced by ribosome or its small subunit...
  4. Sermon J, Vanoirbeek K, De Spiegeleer P, Van Houdt R, Aertsen A, Michiels C. Unique stress response to the lactoperoxidase-thiocyanate enzyme system in Escherichia coli. Res Microbiol. 2005;156:225-32 pubmed
    ..Several of the induced genes or pathways may be involved in bacterial defense against the toxic effects of the lactoperoxidase-thiocyanate enzyme system. ..
  5. Nichols C, Johnson C, Lamb H, Lockyer M, Charles I, Hawkins A, et al. Structure of the ribosomal interacting GTPase YjeQ from the enterobacterial species Salmonella typhimurium. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007;63:922-8 pubmed
    The YjeQ class of P-loop GTPases assist in ribosome biogenesis and also bind to the 30S subunit of mature ribosomes...
  6. Kimura T, Takagi K, Kyoko H, Kalachnyuk L, Muto A, Himeno H. Interaction between RsgA and the ribosome. Nucleic Acids Symp Ser (Oxf). 2007;:375-6 pubmed
    b>RsgA is a unique GTP hydrolytic protein that is widely found in bacteria and plants, and is activated by the small subunit of the ribosome...
  7. Campbell T, Brown E. Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis. J Bacteriol. 2008;190:2537-45 pubmed publisher
    The Escherichia coli protein YjeQ is a circularly permuted GTPase that is broadly conserved in bacteria...
  8. Kimura T, Takagi K, Hirata Y, Hase Y, Muto A, Himeno H. Ribosome-small-subunit-dependent GTPase interacts with tRNA-binding sites on the ribosome. J Mol Biol. 2008;381:467-77 pubmed publisher
    RsgA (ribosome-small-subunit-dependent GTPase A, also known as YjeQ) is a unique GTPase in that guanosine triphosphate hydrolytic activity is activated by the small subunit of the ribosome...
  9. Hase Y, Yokoyama S, Muto A, Himeno H. Removal of a ribosome small subunit-dependent GTPase confers salt resistance on Escherichia coli cells. RNA. 2009;15:1766-74 pubmed publisher
    b>RsgA is a unique GTP hydrolytic protein in which GTPase activity is significantly enhanced by the small ribosomal subunit...

More Information


  1. Jomaa A, Stewart G, Mears J, Kireeva I, Brown E, Ortega J. Cryo-electron microscopy structure of the 30S subunit in complex with the YjeQ biogenesis factor. RNA. 2011;17:2026-38 pubmed publisher
    b>YjeQ is a protein broadly conserved in bacteria containing an N-terminal oligonucleotide/oligosaccharide fold (OB-fold) domain, a central GTPase domain, and a C-terminal zinc-finger domain...
  2. Jeganathan A, Razi A, Thurlow B, Ortega J. The C-terminal helix in the YjeQ zinc-finger domain catalyzes the release of RbfA during 30S ribosome subunit assembly. RNA. 2015;21:1203-16 pubmed publisher
    YjeQ (also called RsgA) and RbfA proteins in Escherichia coli bind to immature 30S ribosome subunits at late stages of assembly to assist folding of the decoding center...
  3. Razi A, Guarné A, Ortega J. The cryo-EM structure of YjeQ bound to the 30S subunit suggests a fidelity checkpoint function for this protein in ribosome assembly. Proc Natl Acad Sci U S A. 2017;114:E3396-E3403 pubmed publisher
    Recent work suggests that bacterial YjeQ (RsgA) participates in the late stages of assembly of the 30S subunit and aids the assembly of the decoding center but also binds the mature 30S subunit with high affinity...
  4. Campbell T, Daigle D, Brown E. Characterization of the Bacillus subtilis GTPase YloQ and its role in ribosome function. Biochem J. 2005;389:843-52 pubmed
    ..of the cellular phenotype and biochemical activity of a conserved bacterial GTPase of unknown function (YloQ and YjeQ in Bacillus subtilis and Escherichia coli respectively) using a collection of antibiotics of diverse mechanisms and ..
  5. Comartin D, Brown E. Non-ribosomal factors in ribosome subunit assembly are emerging targets for new antibacterial drugs. Curr Opin Pharmacol. 2006;6:453-8 pubmed
    ..Accumulating evidence indicates that the proteins Era, Obg, YjeQ, YlqF and RimM are chaperones which may be crucial to bacterial ribosome assembly, and therefore represent novel ..
  6. Jomaa A, Stewart G, Martín Benito J, Zielke R, Campbell T, Maddock J, et al. Understanding ribosome assembly: the structure of in vivo assembled immature 30S subunits revealed by cryo-electron microscopy. RNA. 2011;17:697-709 pubmed publisher
    ..Here, we used a viable deletion in the yjeQ gene, a recognized ribosome assembly factor, to isolate and structurally characterize immature 30S subunits ..