Gene Symbol: rpsR
Description: 30S ribosomal subunit protein S18
Alias: ECK4198, JW4160
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Cooperman B, Grant P, Goldman R, Luddy M, Minnella A, Nicholson A, et al. Photoaffinity labeling of ribosomes. Methods Enzymol. 1979;59:796-815 pubmed
  2. Yaguchi M. Primary structure of protein S18 from the small Escherichia coli ribosomal subunit. FEBS Lett. 1975;59:217-20 pubmed
  3. Gu S, Jöckel J, Beinker P, Warnecke J, Semenkov Y, Rodnina M, et al. Conformation of 4.5S RNA in the signal recognition particle and on the 30S ribosomal subunit. RNA. 2005;11:1374-84 pubmed
    ..The functional role of 4.5S RNA binding to the 30S subunit is unclear, as the RNA had no effect on translation or tRNA translocation on the ribosome. ..
  4. Daigle D, Brown E. Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro. J Bacteriol. 2004;186:1381-7 pubmed
    ..Taken together, these data indicate that the YjeQ protein participates in a guanine nucleotide-dependent interaction with the ribosome and implicate this conserved, essential GTPase as a novel factor in ribosome function. ..
  5. Cooperman B, Expert Bezançon A, Kahan L, Dondon J, Grunberg Manago M. IF-3 crosslinking to Escherichia coli ribosomal 30 S subunits by three different light-dependent procedures: identification of 30 S proteins crosslinked to IF-3--utilization of a new two-stage crosslinking reagent, p-nitrobenzylmaleimide. Arch Biochem Biophys. 1981;208:554-62 pubmed
  6. Gregory R, Zeller M, Thurlow D, Gourse R, Stark M, Dahlberg A, et al. Interaction of ribosomal proteins S6, S8, S15 and S18 with the central domain of 16 S ribosomal RNA from Escherichia coli. J Mol Biol. 1984;178:287-302 pubmed
    ..abstract truncated at 400 words) ..
  7. Matelska D, Purta E, Panek S, Boniecki M, Bujnicki J, Dunin Horkawicz S. S6:S18 ribosomal protein complex interacts with a structural motif present in its own mRNA. RNA. 2013;19:1341-8 pubmed publisher
    ..These results provide a molecular basis for understanding protein-RNA recognition and suggest that the S6S18CBM is involved in an auto-regulatory mechanism. ..
  8. Isono K, Kitakawa M. Cluster of ribosomal protein genes in Escherichia coli containing genes for proteins S6, S18, and L9. Proc Natl Acad Sci U S A. 1978;75:6163-7 pubmed
    ..coli genetic map together with the genes coding for two other ribosomal proteins, S6 (rpsF) and S18 (rpsR). Furthermore, the region of the E...
  9. Pathania R, Zlitni S, Barker C, Das R, Gerritsma D, Lebert J, et al. Chemical genomics in Escherichia coli identifies an inhibitor of bacterial lipoprotein targeting. Nat Chem Biol. 2009;5:849-56 pubmed publisher
    ..We show that MAC13243 inhibits the function of the LolA protein and represents a new chemical probe of lipoprotein targeting in bacteria with promise as an antibacterial lead with Gram-negative selectivity. ..

More Information


  1. Isono K, Isono S. Ribosomal protein modification in Escherichia coli. II. Studies of a mutant lacking the N-terminal acetylation of protein S18. Mol Gen Genet. 1980;177:645-51 pubmed
    ..The mutation does not affect the acetylation of two other ribosomal proteins, S5 and L12, both of which are known to be acetylated in wild-type E. coli K12. ..
  2. Dabbs E. Kasugamycin-dependent mutants of Escherichia coli. J Bacteriol. 1978;136:994-1001 pubmed
    ..Three showed changes in protein S9. Each of two mutants had changes in two proteins, S18 and L11. Three of these mutants additionally had protein S18 occurring in a partly altered, partly unaltered form. ..
  3. Himeno H, Hanawa Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, et al. A novel GTPase activated by the small subunit of ribosome. Nucleic Acids Res. 2004;32:5303-9 pubmed
    ..We also found that 17S RNA, a putative precursor of 16S rRNA, was contained in the small subunit of the ribosome from the RsgA-deletion strain. RsgA is a novel GTPase that might provide a new insight into the function of ribosome. ..
  4. Bischof O, Kruft V, Wittmann Liebold B. Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level. J Biol Chem. 1994;269:18315-9 pubmed
    ..For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level. ..
  5. Nicholson A, Hall C, Strycharz W, Cooperman B. Photoaffinity labeling of Escherichia coli ribosomes by an aryl azide analogue of puromycin. Evidence for the functional site specificity of labeling. Biochemistry. 1982;21:3809-17 pubmed
    ..The possible relationship of the major proteins labeled, S18, S7, and S14, to tRNA binding is considered. ..
  6. Hindennach I, Stoffler G, Wittmann H. Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli. Eur J Biochem. 1971;23:7-11 pubmed
  7. Ginzburg I, Miskin R, Zamir A. N-ethyl maleimide as a probe for the study of functional sites and conformations of 30 S ribosomal subunits. J Mol Biol. 1973;79:481-94 pubmed
  8. Stern S, Powers T, Changchien L, Noller H. Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA. J Mol Biol. 1988;201:683-95 pubmed
    ..Altogether these results show that many of the small subunit proteins, which have previously been shown to be functionally important, appear to be associated with functionally implicated segments of 16 S rRNA. ..
  9. Moine H, Bienaime C, Mougel M, Reinbolt J, Ebel J, Ehresmann C, et al. Crosslinking of ribosomal protein S18 to 16 S RNA in E.coli ribosomal 30 S subunits by the use of a reversible crosslinking agent: trans-diamminedichloroplatinum(II). FEBS Lett. 1988;228:1-6 pubmed
    ..This result is discussed in the light of current knowledge of the topographical localization of S18 in the 30 S subunit and of its relation with function. ..
  10. Boileau G, Butler P, Hershey J, Traut R. Direct cross-links between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolane. Biochemistry. 1983;22:3162-70 pubmed
    ..Cross-links between factors IF1-IF2 and IF3-IF2 were also identified. A model integrating these findings with others on the protein topography of the ribosome is presented. ..
  11. MacKeen L, Kahan L, Wahba A, Schwartz I. Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunits. J Biol Chem. 1980;255:10526-31 pubmed
    ..The target proteins have been identified as S7, S11, S12, S18, and S21 by immunochemical techniques. ..
  12. Held W, Mizushima S, Nomura M. Reconstitution of Escherichia coli 30 S ribosomal subunits from purified molecular components. J Biol Chem. 1973;248:5720-30 pubmed
  13. Schnier J, Kitakawa M, Isono K. The nucleotide sequence of an Escherichia coli chromosomal region containing the genes for ribosomal proteins S6, S18, L9 and an open reading frame. Mol Gen Genet. 1986;204:126-32 pubmed
    The DNA sequence of a cluster of genes for ribosomal proteins S6 (rpsF), S18 (rpsR) and L9 (rplI), and of their surrounding regions was determined. The order of the genes was established as promoter-rpsF-rpsR-rplI...
  14. Noller H, Hoang L, Fredrick K. The 30S ribosomal P site: a function of 16S rRNA. FEBS Lett. 2005;579:855-8 pubmed
    ..Deletion of these tails now shows that the 16S rRNA contacts alone are sufficient to support protein synthesis in living cells. ..
  15. Allen G, Kornberg A. The priB gene encoding the primosomal replication n protein of Escherichia coli. J Biol Chem. 1991;266:11610-3 pubmed
    The gene encoding protein n of the Escherichia coli primosome has been discovered in the rpsF-rpsR-rplI ribosomal protein operon and designated priB...
  16. Adilakshmi T, Bellur D, Woodson S. Concurrent nucleation of 16S folding and induced fit in 30S ribosome assembly. Nature. 2008;455:1268-72 pubmed publisher
    ..Although early steps in assembly are linked to intrinsically stable rRNA structure, later steps correspond to regions of induced fit between the proteins and the rRNA. ..
  17. Isono K, Krauss J, Hirota Y. Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Mol Gen Genet. 1976;149:297-302 pubmed
    ..The importance of these mutants for structural and functional analyses of ribosomes is discussed. ..
  18. Nicholson A, Cooperman B. Photoaffinity labeling of Escherichia coli ribosomes with an aryl azide analogue of puromycin. FEBS Lett. 1978;90:203-8 pubmed
  19. Hauser R, Ceol A, Rajagopala S, Mosca R, Siszler G, Wermke N, et al. A second-generation protein-protein interaction network of Helicobacter pylori. Mol Cell Proteomics. 2014;13:1318-29 pubmed publisher
    ..coli of which one third turned out to be conserved in both species. ..