Gene Symbol: rpsO
Description: 30S ribosomal subunit protein S15
Alias: ECK3154, JW3134, secC
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Portier C, Dondon L, Grunberg Manago M. Translational autocontrol of the Escherichia coli ribosomal protein S15. J Mol Biol. 1990;211:407-14 pubmed
    When rpsO, the gene encoding the ribosomal protein S15 in Escherichia coli, is carried by a multicopy plasmid, the mRNA synthesis rate of S15 increases with the gene dosage but the rate of synthesis of S15 does not rise...
  2. Portier C, Regnier P. Expression of the rpsO and pnp genes: structural analysis of a DNA fragment carrying their control regions. Nucleic Acids Res. 1984;12:6091-102 pubmed
    Precise physical mapping of the genes rpsO and pnp coding respectively for ribosomal protein S15 and polynucleotide phosphorylase together with regions involved in the regulation of their expression has been obtained by the analysis of in ..
  3. Zimmermann R, Muto A, Fellner P, Ehresmann C, Branlant C. Location of ribosomal protein binding sites on 16S ribosomal RNA. Proc Natl Acad Sci U S A. 1972;69:1282-6 pubmed
    ..A preliminary map of the binding sites is presented. ..
  4. Haugel Nielsen J, Hajnsdorf E, Regnier P. The rpsO mRNA of Escherichia coli is polyadenylated at multiple sites resulting from endonucleolytic processing and exonucleolytic degradation. EMBO J. 1996;15:3144-52 pubmed
    The rpsO monocistronic messenger, encoding ribosomal protein S15, is destabilized upon polyadenylation occurring at the hairpin structure of the transcription terminator t1...
  5. Mougel M, Philippe C, Ebel J, Ehresmann B, Ehresmann C. The E. coli 16S rRNA binding site of ribosomal protein S15: higher-order structure in the absence and in the presence of the protein. Nucleic Acids Res. 1988;16:2825-39 pubmed
  6. Yano R, Yura T. Suppression of the Escherichia coli rpoH opal mutation by ribosomes lacking S15 protein. J Bacteriol. 1989;171:1712-7 pubmed
    ..Genetic mapping and complementation studies permitted us to localize suhD near rpsO (69 min), the structural gene for ribosomal protein S15...
  7. Ferro Novick S, Honma M, Beckwith J. The product of gene secC is involved in the synthesis of exported proteins in E. coli. Cell. 1984;38:211-7 pubmed
    ..coli we have isolated suppressors of a mutant (secAts) that is temperature-sensitive for secretion. One of these, secC, can suppress the secretion defect of secA and has a phenotype of its own...
  8. Plumbridge J, Howe J, Springer M, Touati Schwartz D, Hershey J, Grunberg Manago M. Cloning and mapping of a gene for translational initiation factor IF2 in Escherichia coli. Proc Natl Acad Sci U S A. 1982;79:5033-7 pubmed
    ..coli chromosome, inf B has been located at 68 min, very close to argG, nusA, rpsO, and pnp...
  9. Ehresmann C, Ehresmann B, Ennifar E, Dumas P, Garber M, Mathy N, et al. Molecular mimicry in translational regulation: the case of ribosomal protein S15. RNA Biol. 2004;1:66-73 pubmed
    ..They also highlight the high plasticity of RNA to adapt to evolutionary constraints. ..

More Information


  1. Folichon M, Arluison V, Pellegrini O, Huntzinger E, Regnier P, Hajnsdorf E. The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation. Nucleic Acids Res. 2003;31:7302-10 pubmed
    ..In this paper, we study the ability of the Escherichia coli Hfq protein to bind to a polyadenylated fragment of rpsO mRNA. Hfq exhibits a high specificity for a 100-nucleotide RNA harboring 18 3'-terminal A-residues...
  2. Ehresmann C, Philippe C, Westhof E, Benard L, Portier C, Ehresmann B. A pseudoknot is required for efficient translational initiation and regulation of the Escherichia coli rpsO gene coding for ribosomal protein S15. Biochem Cell Biol. 1995;73:1131-40 pubmed
    ..Based on footprinting experiments and computer graphic modelling, S15 shields the two stems of the pseudoknot, sitting in the major groove of the coaxial stack. ..
  3. Portier C. Physical localisation and direction of transcription of the structural gene for Escherichia coli ribosomal protein S15. Gene. 1982;18:261-6 pubmed
    The coding sequence for the Escherichia coli ribosomal protein S15 (rpsO) has been shown to lie immediately adjacent to the structural gene for polynucleotide phosphorylase (pnp)...
  4. Gregory R, Zeller M, Thurlow D, Gourse R, Stark M, Dahlberg A, et al. Interaction of ribosomal proteins S6, S8, S15 and S18 with the central domain of 16 S ribosomal RNA from Escherichia coli. J Mol Biol. 1984;178:287-302 pubmed
    ..abstract truncated at 400 words) ..
  5. Hindennach I, Stoffler G, Wittmann H. Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli. Eur J Biochem. 1971;23:7-11 pubmed
  6. Katayama A, Tsujii A, Wada A, Nishino T, Ishihama A. Systematic search for zinc-binding proteins in Escherichia coli. Eur J Biochem. 2002;269:2403-13 pubmed
    ..TktA/TktB), translation elongation factor Ts (Tsf), ribosomal proteins L2 (RplB), L13 (RplM) and one of S15 (RpsO), S16 (RpsP) or S17 (RpsQ)...
  7. Jagannathan I, Culver G. Assembly of the central domain of the 30S ribosomal subunit: roles for the primary binding ribosomal proteins S15 and S8. J Mol Biol. 2003;330:373-83 pubmed
    ..Thus, while S8 binding is not absolutely required for assembly of the platform, it appears to affect significantly the 16S rRNA environment of S15 by influencing central domain organization. ..
  8. Adilakshmi T, Bellur D, Woodson S. Concurrent nucleation of 16S folding and induced fit in 30S ribosome assembly. Nature. 2008;455:1268-72 pubmed publisher
    ..Although early steps in assembly are linked to intrinsically stable rRNA structure, later steps correspond to regions of induced fit between the proteins and the rRNA. ..
  9. Marzi S, Myasnikov A, Serganov A, Ehresmann C, Romby P, Yusupov M, et al. Structured mRNAs regulate translation initiation by binding to the platform of the ribosome. Cell. 2007;130:1019-31 pubmed
    ..Different types of mRNA structures may be accommodated during translation preinitiation and regulate gene expression by transiently stalling the ribosome. ..
  10. Wiener L, Schuler D, Brimacombe R. Protein binding sites on Escherichia coli 16S ribosomal RNA; RNA regions that are protected by proteins S7, S9 and S19, and by proteins S8, S15 and S17. Nucleic Acids Res. 1988;16:1233-50 pubmed
    ..When protein S15 was omitted, S8 and S18 showed protection of part of helix 44 in addition to the latter regions. The results are discussed in terms of our model for the detailed arrangement of proteins and RNA in the 30S subunit. ..
  11. Serganov A, Ennifar E, Portier C, Ehresmann B, Ehresmann C. Do mRNA and rRNA binding sites of E.coli ribosomal protein S15 share common structural determinants?. J Mol Biol. 2002;320:963-78 pubmed
    ..Otherwise, unique specific features are utilized, such as the three-way junction in the case of 16S rRNA and the looped out A(-46) for the mRNA pseudoknot. ..
  12. Philippe C, Eyermann F, Benard L, Portier C, Ehresmann B, Ehresmann C. Ribosomal protein S15 from Escherichia coli modulates its own translation by trapping the ribosome on the mRNA initiation loading site. Proc Natl Acad Sci U S A. 1993;90:4394-8 pubmed
    ..Our results indicate that S15 prevents the formation of a functional ternary 30S-mRNA-tRNA(fMet) complex, the ribosome being trapped in a preternary 30S-mRNA-tRNA(fMet) complex. ..
  13. Hajnsdorf E, Steier O, Coscoy L, Teysset L, Regnier P. Roles of RNase E, RNase II and PNPase in the degradation of the rpsO transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant. EMBO J. 1994;13:3368-77 pubmed
    The Escherichia coli rpsO gene gives rise to different mRNA species resulting either from termination of transcription or from processing of primary transcripts by RNase E and RNase III...
  14. R gnier P, Grunberg Manago M, Portier C. Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase. Homology of the primary structure of the protein with the RNA-binding domain of ribosomal protein S1. J Biol Chem. 1987;262:63-8 pubmed
    The pnp gene is located at 69 min on the Escherichia coli chromosome adjacent to the rpsO gene which encodes the ribosomal protein S15...
  15. Sands J, Regnier P, Cummings H, Grunberg Manago M, Hershey J. The existence of two genes between infB and rpsO in the Escherichia coli genome: DNA sequencing and S1 nuclease mapping. Nucleic Acids Res. 1988;16:10803-16 pubmed
    ..as two operons: the metY operon, containing metY, P15A, nusA, infB; and about a kilobase further downstream, the rpsO and pnp operon...
  16. Wittmann Liebold B. Studies on the primary structure of 20 proteins from Escherichia coli ribosomes by means of an improved protein sequenator. FEBS Lett. 1973;36:247-9 pubmed
  17. Hajnsdorf E, Braun F, Haugel Nielsen J, Regnier P. Polyadenylylation destabilizes the rpsO mRNA of Escherichia coli. Proc Natl Acad Sci U S A. 1995;92:3973-7 pubmed
    The rpsO mRNA, encoding ribosomal protein S15, is only partly stabilized when the three ribonucleases implicated in its degradation--RNase E, polynucleotide phosphorylase, and RNase II--are inactivated...
  18. Le Derout J, Regnier P, Hajnsdorf E. Both temperature and medium composition regulate RNase E processing efficiency of the rpsO mRNA coding for ribosomal protein S15 of Escherichia coli. J Mol Biol. 2002;319:341-9 pubmed
    ..We report that rpsO mRNA, 15 minutes after a shift to 44 degrees C, is stabilized in cells grown in minimal medium...
  19. Benard L, Mathy N, Grunberg Manago M, Ehresmann B, Ehresmann C, Portier C. Identification in a pseudoknot of a U.G motif essential for the regulation of the expression of ribosomal protein S15. Proc Natl Acad Sci U S A. 1998;95:2564-7 pubmed
    ..A unique motif, made of only two adjacent base pairs, U.G/C.G, is essential for S15 autoregulation and is presumably involved in direct recognition by the S15 protein. ..
  20. Kitakawa M, Dabbs E, Isono K. Genes coding for ribosomal proteins S15, L21, and L27 map near argG in Escherichia coli. J Bacteriol. 1979;138:832-8 pubmed
    ..1 min, whereas the gene for S15 (rpsO) is situated close to, but on the opposite side or, argG...
  21. Philippe C, Benard L, Eyermann F, Cachia C, Kirillov S, Portier C, et al. Structural elements of rps0 mRNA involved in the modulation of translational initiation and regulation of E. coli ribosomal protein S15. Nucleic Acids Res. 1994;22:2538-46 pubmed
  22. Benard L, Philippe C, Dondon L, Grunberg Manago M, Ehresmann B, Ehresmann C, et al. Mutational analysis of the pseudoknot structure of the S15 translational operator from Escherichia coli. Mol Microbiol. 1994;14:31-40 pubmed
    Expression of rpsO, the gene encoding the small ribosomal protein S15, is autoregulated at the translational level by S15, which binds to its mRNA in a region overlapping the ribosome-binding site...
  23. Watanabe T, Hayashi S, Wu H. Synthesis and export of the outer membrane lipoprotein in Escherichia coli mutants defective in generalized protein export. J Bacteriol. 1988;170:4001-7 pubmed
    ..examined in conditionally lethal mutants that were defective in protein export in general, including secA, secB, secC, and secD. Lipoprotein export was affected in a secA(Ts) mutant of E...
  24. Ying B, Suzuki T, Shimizu Y, Ueda T. A novel screening system for self-mRNA targeting proteins. J Biochem. 2003;133:485-91 pubmed
  25. Portier C, Philippe C, Dondon L, Grunberg Manago M, Ebel J, Ehresmann B, et al. Translational control of ribosomal protein S15. Biochim Biophys Acta. 1990;1050:328-36 pubmed
    ..Binding of S15 to these two domains suggests that the pseudoknot could be stabilized by S15. A model is presented in which two alternative structures would explain the molecular basis of the S15 autocontrol. ..
  26. Boehringer D, Ban N. Trapping the ribosome to control gene expression. Cell. 2007;130:983-5 pubmed
    ..These results have implications for our understanding of the mechanism of translation initiation in general. ..
  27. Mathy N, Pellegrini O, Serganov A, Patel D, Ehresmann C, Portier C. Specific recognition of rpsO mRNA and 16S rRNA by Escherichia coli ribosomal protein S15 relies on both mimicry and site differentiation. Mol Microbiol. 2004;52:661-75 pubmed
    The ribosomal protein S15 binds to 16S rRNA, during ribosome assembly, and to its own mRNA (rpsO mRNA), affecting autocontrol of its expression...
  28. Folichon M, Marujo P, Arluison V, Le Derout J, Pellegrini O, Hajnsdorf E, et al. Fate of mRNA extremities generated by intrinsic termination: detailed analysis of reactions catalyzed by ribonuclease II and poly(A) polymerase. Biochimie. 2005;87:819-26 pubmed
    ..The detailed analysis of the rpsO mRNA of Escherichia coli presented here demonstrates that transcription terminates in vivo at two sites located ..
  29. Regnier P, Hajnsdorf E. Decay of mRNA encoding ribosomal protein S15 of Escherichia coli is initiated by an RNase E-dependent endonucleolytic cleavage that removes the 3' stabilizing stem and loop structure. J Mol Biol. 1991;217:283-92 pubmed
    The transcripts of the rpsO-pnp operon of Escherichia coli, coding for ribosomal protein S15 and polynucleotide phosphorylase, are processed at four sites in the 249 nucleotides of the intercistronic region...
  30. Braun F, Hajnsdorf E, Regnier P. Polynucleotide phosphorylase is required for the rapid degradation of the RNase E-processed rpsO mRNA of Escherichia coli devoid of its 3' hairpin. Mol Microbiol. 1996;19:997-1005 pubmed
    The monocistronic transcript of rpsO undergoes an endonucleolytic cleavage downstream of the coding sequence, which removes the hairpin of the transcription terminator and initiates the rapid degradation of the message...
  31. Regnier P, Portier C. Initiation, attenuation and RNase III processing of transcripts from the Escherichia coli operon encoding ribosomal protein S15 and polynucleotide phosphorylase. J Mol Biol. 1986;187:23-32 pubmed
    The rpsO gene of Escherichia coli, which encodes ribosomal protein S15 is located at 69 minutes on the chromosome. It is adjacent to the pnp gene, which encodes polynucleotide phosphorylase...
  32. Stark M, Gregory R, Gourse R, Thurlow D, Zwieb C, Zimmermann R, et al. Effects of site-directed mutations in the central domain of 16 S ribosomal RNA upon ribosomal protein binding, RNA processing and 30 S subunit assembly. J Mol Biol. 1984;178:303-22 pubmed
    ..Moreover, we have confirmed the essentiality of certain rRNA sequences for the formation and/or stabilization of these protein-rRNA interactions.(ABSTRACT TRUNCATED AT 400 WORDS) ..
  33. Himeno H, Hanawa Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, et al. A novel GTPase activated by the small subunit of ribosome. Nucleic Acids Res. 2004;32:5303-9 pubmed
    ..We also found that 17S RNA, a putative precursor of 16S rRNA, was contained in the small subunit of the ribosome from the RsgA-deletion strain. RsgA is a novel GTPase that might provide a new insight into the function of ribosome. ..
  34. Serganov A, Polonskaia A, Ehresmann B, Ehresmann C, Patel D. Ribosomal protein S15 represses its own translation via adaptation of an rRNA-like fold within its mRNA. EMBO J. 2003;22:1898-908 pubmed
    ..Our results highlight an astonishing plasticity of mRNA in its ability to adapt to evolutionary constraints, that contrasts with the extreme conservation of the rRNA-binding site. ..
  35. Morinaga T, Funatsu G, Funatsu M, Wittman H. Primary structure of the 16S rRNA binding protein S15 from Escherichia coli ribosomes. FEBS Lett. 1976;64:307-9 pubmed
  36. Zimmermann R, Mackie G, Muto A, Garrett R, Ungewickell E, Ehresmann C, et al. Location and characteristics of ribosomal protein binding sites in the 16S RNA of Escherichia coli. Nucleic Acids Res. 1975;2:279-302 pubmed
    ..Evidence is presented which indicates that additional proteins interact with the RNA at later stages of subunit assembly. ..
  37. Takata R, Mukai T, Aoyagi M, Hori K. Nucleotide sequence of the gene for Escherichia coli ribosomal protein S15 (rpsO). Mol Gen Genet. 1984;197:225-9 pubmed
    The nucleotide sequence of the ribosomal protein gene rpsO (S15) and its flanking region were determined...
  38. Mizushima S, Nomura M. Assembly mapping of 30S ribosomal proteins from E. coli. Nature. 1970;226:1214 pubmed
  39. Braun F, Le Derout J, Regnier P. Ribosomes inhibit an RNase E cleavage which induces the decay of the rpsO mRNA of Escherichia coli. EMBO J. 1998;17:4790-7 pubmed
    ..These experiments used an rpsO mRNA deleted of the translational operator where ribosomal protein S15 autoregulates its synthesis...
  40. Takata R, Mukai T, Hori K. Attenuation and processing of RNA from the rpsO-pnp transcription unit of Escherichia coli. Nucleic Acids Res. 1985;13:7289-97 pubmed
    Ribosomal protein S15 and polynucleotide phosphorylase of E. coli are encoded by two adjacent genes, rpsO and pnp, respectively. Analysis of in vivo transcripts from these two genes shows that they are within the same operon (S15 operon)...
  41. Marujo P, Braun F, Haugel Nielsen J, Le Derout J, Arraiano C, Regnier P. Inactivation of the decay pathway initiated at an internal site by RNase E promotes poly(A)-dependent degradation of the rpsO mRNA in Escherichia coli. Mol Microbiol. 2003;50:1283-94 pubmed
    ..The dominant pathway of decay of the rpsO transcripts is initiated by an RNase E cleavage occurring at a preferential site named M2...
  42. Noller H, Hoang L, Fredrick K. The 30S ribosomal P site: a function of 16S rRNA. FEBS Lett. 2005;579:855-8 pubmed
    ..Deletion of these tails now shows that the 16S rRNA contacts alone are sufficient to support protein synthesis in living cells. ..
  43. Held W, Mizushima S, Nomura M. Reconstitution of Escherichia coli 30 S ribosomal subunits from purified molecular components. J Biol Chem. 1973;248:5720-30 pubmed
  44. Andrade J, Hajnsdorf E, Regnier P, Arraiano C. The poly(A)-dependent degradation pathway of rpsO mRNA is primarily mediated by RNase R. RNA. 2009;15:316-26 pubmed publisher
    ..RNA exonucleases present in Escherichia coli, could not account for all the poly(A)-dependent degradation of the rpsO mRNA...
  45. Isono K, Krauss J, Hirota Y. Isolation and characterization of temperature-sensitive mutants of Escherichia coli with altered ribosomal proteins. Mol Gen Genet. 1976;149:297-302 pubmed
    ..The importance of these mutants for structural and functional analyses of ribosomes is discussed. ..
  46. Bubunenko M, Baker T, Court D. Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli. J Bacteriol. 2007;189:2844-53 pubmed
    ..Interestingly, although most 30S ribosomal proteins were essential, the knockouts of six ribosomal protein genes, rpsF (S6), rpsI (S9), rpsM (S13), rpsO (S15), rpsQ (S17), and rpsT (S20), were viable.
  47. Hajnsdorf E, Regnier P. E. coli RpsO mRNA decay: RNase E processing at the beginning of the coding sequence stimulates poly(A)-dependent degradation of the mRNA. J Mol Biol. 1999;286:1033-43 pubmed
    The rpsO mRNA of E. coli encoding ribosomal protein S15 is destabilized by poly(A) tails posttranscriptionally added by poly(A)polymerase I...
  48. Evans S, Dennis P. Promoter activity and transcript mapping in the regulatory region for genes encoding ribosomal protein S15 and polynucleotide phosphorylase of Escherichia coli. Gene. 1985;40:15-22 pubmed
    The genes encoding ribosomal protein S15 (rpsO) and polynucleotide phosphorylase (pnp) occupy adjacent positions and are oriented in the same direction on the Escherichia coli chromosomes...
  49. Nakamura Y, Mizusawa S. In vivo evidence that the nusA and infB genes of E. coli are part of the same multi-gene operon which encodes at least four proteins. EMBO J. 1985;4:527-32 pubmed
    ..000 (argG), 21 000 (p21), 64 000 (nusA), 120 000 (IF2 alpha)-(infB), 91 000 (IF2 beta)(infB), 15 000 (p15), 10 000 (rpsO) and 85 000 (pnp)...
  50. Bubunenko M, Korepanov A, Court D, Jagannathan I, Dickinson D, Chaudhuri B, et al. 30S ribosomal subunits can be assembled in vivo without primary binding ribosomal protein S15. RNA. 2006;12:1229-39 pubmed
    ..To investigate the role of S15 in vivo, the essential nature of rpsO, the gene encoding S15, was examined. Surprisingly, E...
  51. Springer M, Portier C. More than one way to skin a cat: translational autoregulation by ribosomal protein S15. Nat Struct Biol. 2003;10:420-2 pubmed
  52. Philippe C, Benard L, Portier C, Westhof E, Ehresmann B, Ehresmann C. Molecular dissection of the pseudoknot governing the translational regulation of Escherichia coli ribosomal protein S15. Nucleic Acids Res. 1995;23:18-28 pubmed
    ..S15 sits on the deep groove of the co-axial stack and makes contacts with both stems, shielding the bridging adenine. The only specific sequence determinants are found in the helix common to the pseudoknot and the hairpin structures. ..
  53. Philippe C, Portier C, Mougel M, Grunberg Manago M, Ebel J, Ehresmann B, et al. Target site of Escherichia coli ribosomal protein S15 on its messenger RNA. Conformation and interaction with the protein. J Mol Biol. 1990;211:415-26 pubmed
    ..A mechanism can be postulated in which the regulatory protein stabilizes this particular structure, thus impeding ribosome initiation. ..
  54. Hajnsdorf E, Braun F, Haugel Nielsen J, Le Derout J, Regnier P. Multiple degradation pathways of the rpsO mRNA of Escherichia coli. RNase E interacts with the 5' and 3' extremities of the primary transcript. Biochimie. 1996;78:416-24 pubmed
    The degradation process of the rpsO mRNA is one of the best characterised in E coli. Two independent degradation pathways have been identified...
  55. Daigle D, Brown E. Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro. J Bacteriol. 2004;186:1381-7 pubmed
    ..Taken together, these data indicate that the YjeQ protein participates in a guanine nucleotide-dependent interaction with the ribosome and implicate this conserved, essential GTPase as a novel factor in ribosome function. ..
  56. Serganov A, Benard L, Portier C, Ennifar E, Garber M, Ehresmann B, et al. Role of conserved nucleotides in building the 16 S rRNA binding site for ribosomal protein S15. J Mol Biol. 2001;305:785-803 pubmed
    ..Thus, the role of site 1 is to anchor S15 to the rRNA, while binding at site 2 is aimed to induce a cascade of events required for subunit assembly. ..