Gene Symbol: rpsN
Description: 30S ribosomal subunit protein S14
Alias: ECK3294, JW3269
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Noller H, Hoang L, Fredrick K. The 30S ribosomal P site: a function of 16S rRNA. FEBS Lett. 2005;579:855-8 pubmed
    ..Deletion of these tails now shows that the 16S rRNA contacts alone are sufficient to support protein synthesis in living cells. ..
  2. Cooperman B, Grant P, Goldman R, Luddy M, Minnella A, Nicholson A, et al. Photoaffinity labeling of ribosomes. Methods Enzymol. 1979;59:796-815 pubmed
  3. Himeno H, Hanawa Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, et al. A novel GTPase activated by the small subunit of ribosome. Nucleic Acids Res. 2004;32:5303-9 pubmed
    ..We also found that 17S RNA, a putative precursor of 16S rRNA, was contained in the small subunit of the ribosome from the RsgA-deletion strain. RsgA is a novel GTPase that might provide a new insight into the function of ribosome. ..
  4. Powers T, Stern S, Changchien L, Noller H. Probing the assembly of the 3' major domain of 16 S rRNA. Interactions involving ribosomal proteins S2, S3, S10, S13 and S14. J Mol Biol. 1988;201:697-716 pubmed
    ..These results, in combination with the results from our previous study of proteins S7, S9 and S19, are discussed in terms of the assembly, topography and involvement in ribosomal function of the 3' major domain. ..
  5. Daigle D, Brown E. Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro. J Bacteriol. 2004;186:1381-7 pubmed
    ..Taken together, these data indicate that the YjeQ protein participates in a guanine nucleotide-dependent interaction with the ribosome and implicate this conserved, essential GTPase as a novel factor in ribosome function. ..
  6. Nicholson A, Hall C, Strycharz W, Cooperman B. Photoaffinity labeling of Escherichia coli ribosomes by an aryl azide analogue of puromycin. Evidence for the functional site specificity of labeling. Biochemistry. 1982;21:3809-17 pubmed
    ..The possible relationship of the major proteins labeled, S18, S7, and S14, to tRNA binding is considered. ..
  7. Hindennach I, Stoffler G, Wittmann H. Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli. Eur J Biochem. 1971;23:7-11 pubmed
  8. Graifer D, Babkina G, Matasova N, Vladimirov S, Karpova G, Vlassov V. Structural arrangement of tRNA binding sites on Escherichia coli ribosomes, as revealed from data on affinity labelling with photoactivatable tRNA derivatives. Biochim Biophys Acta. 1989;1008:146-56 pubmed
  9. Hoving S, Munchbach M, Schmid H, Signor L, Lehmann A, Staudenmann W, et al. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal Chem. 2000;72:1006-14 pubmed
    ..We show that chemical tagging is a viable first-pass high-throughput identification method to be used prior to an in depth MS/MS analysis. ..

Scientific Experts

More Information


  1. Cerretti D, Dean D, Davis G, Bedwell D, Nomura M. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 1983;11:2599-616 pubmed
    ..contains the genes for ten ribosomal proteins: L14, L24, L5, S14, S8, L6, L18, S5, L30 and L15 (rp1N, rp1X, rp1E, rpsN, rpsH, rp1F, rp1R, rpsE, rpmD, and rp1O). We now report the entire 5.9 kb nucleotide sequence of the spc operon...
  2. Nicholson A, Cooperman B. Photoaffinity labeling of Escherichia coli ribosomes with an aryl azide analogue of puromycin. FEBS Lett. 1978;90:203-8 pubmed
  3. Bischof O, Kruft V, Wittmann Liebold B. Analysis of the puromycin binding site in the 70 S ribosome of Escherichia coli at the peptide level. J Biol Chem. 1994;269:18315-9 pubmed
    ..For the first time the molecular environment of an antibiotic in the procaryotic ribosome is presented at the peptide level. ..
  4. Wittmann Liebold B. Studies on the primary structure of 20 proteins from Escherichia coli ribosomes by means of an improved protein sequenator. FEBS Lett. 1973;36:247-9 pubmed
  5. Weitzmann C, Cooperman B. On the structural specificity of puromycin binding to Escherichia coli ribosomes. Biochemistry. 1985;24:2268-74 pubmed
    ..They also force the conclusion that puromycin photoincorporation proceeds through its adenosyl moiety. ..
  6. Wiener L, Brimacombe R. Protein binding sites on Escherichia coli 16S RNA; RNA regions that are protected by proteins S7, S14 and S19 in the presence or absence of protein S9. Nucleic Acids Res. 1987;15:3653-70 pubmed
    ..The results are discussed in terms of the three-dimensional folding of 16S RNA in the 30S subunit. ..
  7. Jaynes E, Grant P, Giangrande G, Wieder R, Cooperman B. Photoinduced affinity labeling of the Escherichia coli ribosome puromycin site. Biochemistry. 1978;17:561-9 pubmed
    ..The detailed photochemical mechanism is not understood, although 8-alkylation of purine moiety has been excluded. Incorporation is largely inhibited in the presence of thiol reagents. ..
  8. Bischof O, Urlaub H, Kruft V, Wittmann Liebold B. Peptide environment of the peptidyl transferase center from Escherichia coli 70 S ribosomes as determined by thermoaffinity labeling with dihydrospiramycin. J Biol Chem. 1995;270:23060-4 pubmed
    ..This approach is a valuable tool to characterize the binding site of spiramycin as well as the peptidyl transferase center at the molecular level. ..
  9. Ramakrishnan V, Graziano V, Capel M. A role for proteins S3 and S14 in the 30 S ribosomal subunit. J Biol Chem. 1986;261:15049-52 pubmed
    ..Our results provide evidence that these two proteins are involved in assembly but may not be required once the 30 S subunit has been properly assembled. ..
  10. Held W, Mizushima S, Nomura M. Reconstitution of Escherichia coli 30 S ribosomal subunits from purified molecular components. J Biol Chem. 1973;248:5720-30 pubmed
  11. Kerlavage A, Cooperman B. Reconstitution of Escherichia coli ribosomes containing puromycin-modified S14: functional effects of the photoaffinity labeling of a protein essential for tRNA binding. Biochemistry. 1986;25:8002-10 pubmed
    ..Suitable control experiments strongly indicate that this loss of activity is a direct consequence of puromycin photoincorporation. ..
  12. Cabezon T, Delcuve G, Faelen M, Desmet L, Bollen A. Polarity of amber mutations in ribosomal protein genes of Escherichia coli. J Bacteriol. 1980;141:41-51 pubmed
    ..indicated that both mutations, 3162(Am) and 3161(Am), affect genes coding for ribosomal proteins: rplC (L3) and rpsN (S14), respectively...
  13. Adilakshmi T, Bellur D, Woodson S. Concurrent nucleation of 16S folding and induced fit in 30S ribosome assembly. Nature. 2008;455:1268-72 pubmed publisher
    ..Although early steps in assembly are linked to intrinsically stable rRNA structure, later steps correspond to regions of induced fit between the proteins and the rRNA. ..