rpsK

Summary

Gene Symbol: rpsK
Description: 30S ribosomal subunit protein S11
Alias: ECK3284, JW3259
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Held W, Mizushima S, Nomura M. Reconstitution of Escherichia coli 30 S ribosomal subunits from purified molecular components. J Biol Chem. 1973;248:5720-30 pubmed
  2. Cooperman B, Expert Bezançon A, Kahan L, Dondon J, Grunberg Manago M. IF-3 crosslinking to Escherichia coli ribosomal 30 S subunits by three different light-dependent procedures: identification of 30 S proteins crosslinked to IF-3--utilization of a new two-stage crosslinking reagent, p-nitrobenzylmaleimide. Arch Biochem Biophys. 1981;208:554-62 pubmed
  3. Kamp R, Wittmann Liebold B. Primary structure of protein S11 from Escherichia coli ribosomes. FEBS Lett. 1980;121:117-22 pubmed
  4. Vercruysse M, Köhrer C, Shen Y, Proulx S, Ghosal A, Davies B, et al. Identification of YbeY-Protein Interactions Involved in 16S rRNA Maturation and Stress Regulation in Escherichia coli. MBio. 2016;7: pubmed publisher
    ..e., ribosomal protein S11, Era). In addition, we identified key residues of YbeY involved in the interaction with S11 and propose a possible binding mode of YbeY to the ribosome using in silico docking. ..
  5. Adilakshmi T, Bellur D, Woodson S. Concurrent nucleation of 16S folding and induced fit in 30S ribosome assembly. Nature. 2008;455:1268-72 pubmed publisher
    ..Although early steps in assembly are linked to intrinsically stable rRNA structure, later steps correspond to regions of induced fit between the proteins and the rRNA. ..
  6. Bubunenko M, Baker T, Court D. Essentiality of ribosomal and transcription antitermination proteins analyzed by systematic gene replacement in Escherichia coli. J Bacteriol. 2007;189:2844-53 pubmed
    ..Interestingly, although most 30S ribosomal proteins were essential, the knockouts of six ribosomal protein genes, rpsF (S6), rpsI (S9), rpsM (S13), rpsO (S15), rpsQ (S17), and rpsT (S20), were viable. ..
  7. Robert F, Brakier Gingras L. A functional interaction between ribosomal proteins S7 and S11 within the bacterial ribosome. J Biol Chem. 2003;278:44913-20 pubmed
    ..Altogether, our results demonstrate that S7 and S11 interact in a functional manner and support the notion that protein-protein interactions contribute to the dynamics of the ribosome. ..
  8. Dallas A, Noller H. Interaction of translation initiation factor 3 with the 30S ribosomal subunit. Mol Cell. 2001;8:855-64 pubmed
    ..The N domain neighbors proteins S7 and S11 and may interfere with E site tRNA binding. Our model suggests that IF3 influences initiator tRNA selection indirectly. ..
  9. Powers T, Noller H. Hydroxyl radical footprinting of ribosomal proteins on 16S rRNA. RNA. 1995;1:194-209 pubmed
    ..These results reinforce and extend previous data on the localization of ribosomal proteins with respect to structural features of 16S rRNA, and offer many new constraints for three-dimensional modeling of the 30S ribosomal subunit. ..

More Information

Publications25

  1. Cerretti D, Dean D, Davis G, Bedwell D, Nomura M. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 1983;11:2599-616 pubmed
  2. Hardy S, Kurland C, Voynow P, Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969;8:2897-905 pubmed
  3. Bedwell D, Davis G, Gosink M, Post L, Nomura M, Kestler H, et al. Nucleotide sequence of the alpha ribosomal protein operon of Escherichia coli. Nucleic Acids Res. 1985;13:3891-903 pubmed
    ..We report here the complete 3.0 kb nucleotide sequence of the alpha operon. In addition, we have determined by S1 nuclease mapping the site of transcription termination in this operon. ..
  4. Stern S, Powers T, Changchien L, Noller H. Interaction of ribosomal proteins S5, S6, S11, S12, S18 and S21 with 16 S rRNA. J Mol Biol. 1988;201:683-95 pubmed
    ..Altogether these results show that many of the small subunit proteins, which have previously been shown to be functionally important, appear to be associated with functionally implicated segments of 16 S rRNA. ..
  5. Boileau G, Butler P, Hershey J, Traut R. Direct cross-links between initiation factors 1, 2, and 3 and ribosomal proteins promoted by 2-iminothiolane. Biochemistry. 1983;22:3162-70 pubmed
    ..Cross-links between factors IF1-IF2 and IF3-IF2 were also identified. A model integrating these findings with others on the protein topography of the ribosome is presented. ..
  6. Meek D, Hayward R. Nucleotide sequence of the rpoA-rplQ DNA of Escherichia coli: a second regulatory binding site for protein S4?. Nucleic Acids Res. 1984;12:5813-21 pubmed
    ..encoding ribosomal proteins (in order of transcription, and with their products named in brackets): rpsM (S13), rpsK (S11), rpsD (S4), rpoA (alpha-subunit of RNA polymerase), rplQ (L17)...
  7. MacKeen L, Kahan L, Wahba A, Schwartz I. Photochemical cross-linking of initiation factor-3 to Escherichia coli 30 S ribosomal subunits. J Biol Chem. 1980;255:10526-31 pubmed
    ..The target proteins have been identified as S7, S11, S12, S18, and S21 by immunochemical techniques. ..
  8. Jaskunas S, Burgess R, Nomura M. Identification of a gene for the alpha-subunit of RNA polymerase at the str-spc region of the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1975;72:5036-40 pubmed
    ..7.7.6) has been identified and mapped between spcA and trkA, near 64 min on the E. coli chromosome. It appears to be coordinately expressed and possibly cotranscribed with the genes for ribosomal proteins S11, S4, and L17. ..
  9. Post L, Arfsten A, Davis G, Nomura M. DNA sequence of the promoter region for the alpha ribosomal protein operon in Escherichia coli. J Biol Chem. 1980;255:4653-59 pubmed
    ..established that the S13 gene is a part of the alpha operon and the gene order is promoter (P alpha), rpsM (S13), rpsK (S11), rpsD(S4), rpoA(alpha), and rplQ(L17). The DNA sequence extending 650 bases before S13 gene was determined...
  10. Rosen K, Zimmerman R. Photoaffinity labeling of 30S-subunit proteins S7 and S11 by 4-thiouridine-substituted tRNA(Phe) situated at the P site of Escherichia coli ribosomes. RNA. 1997;3:1028-36 pubmed
  11. David C, Keener J, Aswad D. Isoaspartate in ribosomal protein S11 of Escherichia coli. J Bacteriol. 1999;181:2872-7 pubmed
    ..The near stoichiometric levels of isoaspartate in S11, estimated at 0.5 mol of isoaspartate per mol of S11, suggests that this unusual modification may be important for S11 function. ..
  12. Hindennach I, Stoffler G, Wittmann H. Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli. Eur J Biochem. 1971;23:7-11 pubmed
  13. Daigle D, Brown E. Studies of the interaction of Escherichia coli YjeQ with the ribosome in vitro. J Bacteriol. 2004;186:1381-7 pubmed
    ..Taken together, these data indicate that the YjeQ protein participates in a guanine nucleotide-dependent interaction with the ribosome and implicate this conserved, essential GTPase as a novel factor in ribosome function. ..
  14. Himeno H, Hanawa Suetsugu K, Kimura T, Takagi K, Sugiyama W, Shirata S, et al. A novel GTPase activated by the small subunit of ribosome. Nucleic Acids Res. 2004;32:5303-9 pubmed
    ..We also found that 17S RNA, a putative precursor of 16S rRNA, was contained in the small subunit of the ribosome from the RsgA-deletion strain. RsgA is a novel GTPase that might provide a new insight into the function of ribosome. ..
  15. Chiaruttini C, Milet M, Hayes D, Expert Bezançon A. Crosslinking of ribosomal proteins S4, S5, S7, S8, S11, S12 and S18 to domains 1 and 2 of 16S rRNA in the Escherichia coli 30S particle. Biochimie. 1989;71:839-52 pubmed
    ..These results are discussed in the light of current knowledge of the tertiary structure of 16S rRNA in the E. coli 30S ribosomal subunit. ..
  16. Noller H, Hoang L, Fredrick K. The 30S ribosomal P site: a function of 16S rRNA. FEBS Lett. 2005;579:855-8 pubmed
    ..Deletion of these tails now shows that the 16S rRNA contacts alone are sufficient to support protein synthesis in living cells. ..