Gene Symbol: rpmF
Description: 50S ribosomal subunit protein L32
Alias: ECK1075, JW1075
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Wittmann Liebold B, Greuer B, Pannenbecker R. The primary structure of protein L32 from the 50S subunit of Escherichia coli ribosomes. Hoppe Seylers Z Physiol Chem. 1975;356:1977-9 pubmed
  2. Tanaka Y, Tsujimura A, Fujita N, Isono S, Isono K. Cloning and analysis of an Escherichia coli operon containing the rpmF gene for ribosomal protein L32 and the gene for a 30-kilodalton protein. J Bacteriol. 1989;171:5707-12 pubmed
    ..The clones were first screened for PyrC+ and then for other genes, including rpmF encoding ribosomal protein L32 that had been mapped near pyrC (I. Janda, M. Kitakawa, and K. Isono, Mol. Gen. Genet...
  3. Janda I, Kitakawa M, Isono K. Gene rpmF for ribosomal protein L32 and gene rimJ for a ribosomal protein acetylating enzyme are located near pyrC (23.4 min) in Escherichia coli. Mol Gen Genet. 1985;201:433-6 pubmed
    ..The mutation leading to this alteration (rpmF) and the temperature-sensitive mutation (ts-1517) present in the same strain were found to map near pyrC (23...
  4. Muralikrishna P, Cooperman B. Ribosomal components neighboring the 2475 loop in Escherichia coli 50S subunits. Biochemistry. 1995;34:115-21 pubmed
    ..The implications of these results for the evolving model of the internal structure of the 50S subunit are considered. ..
  5. Podkovyrov S, Larson T. Lipid biosynthetic genes and a ribosomal protein gene are cotranscribed. FEBS Lett. 1995;368:429-31 pubmed
    By using insertional mutagenesis we demonstrated that the rpmF gene encoding ribosomal protein L32, the plsX gene encoding a protein involved in membrane lipid synthesis and several fatty acid biosynthetic genes (fabH, fabD and fabG) are ..
  6. Zhang Y, Cronan J. Transcriptional analysis of essential genes of the Escherichia coli fatty acid biosynthesis gene cluster by functional replacement with the analogous Salmonella typhimurium gene cluster. J Bacteriol. 1998;180:3295-303 pubmed
    ..approximately 60% of the plsX transcripts initiate at promoters located far upstream and include the upstream rpmF ribosomal protein gene, a promoter located upstream of the plsX coding sequence (probably within the upstream gene, ..
  7. Hoving S, Munchbach M, Schmid H, Signor L, Lehmann A, Staudenmann W, et al. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal Chem. 2000;72:1006-14 pubmed
    ..We show that chemical tagging is a viable first-pass high-throughput identification method to be used prior to an in depth MS/MS analysis. ..
  8. Zhong H, Zhang Y, Wen Z, Li L. Protein sequencing by mass analysis of polypeptide ladders after controlled protein hydrolysis. Nat Biotechnol. 2004;22:1291-6 pubmed
    ..This technique can potentially be automated for large-scale protein annotation. ..
  9. Dovichi N. Climbing the protein ladder. Nat Biotechnol. 2004;22:1242-3 pubmed

More Information


  1. Nakayashiki T, Mori H. Genome-wide screening with hydroxyurea reveals a link between nonessential ribosomal proteins and reactive oxygen species production. J Bacteriol. 2013;195:1226-35 pubmed publisher
    ..These mutants increased the copy number of the p15A-based plasmid and exhibited reduced basal levels of SOS response. The data suggest that nonessential proteins indirectly affect the DNA-damaging process...