Gene Symbol: rpmE
Description: 50S ribosomal subunit protein L31
Alias: ECK3928, JW3907
Species: Escherichia coli str. K-12 substr. MG1655
Brosius J. Primary structure of Escherichia coli ribosomal protein L31. Biochemistry. 1978;17:501-8 pubmed
..Four half-cystine residues were identified at positions 16, 18, 37, and 40. Evidence is presented that suggests that these residues form two disulfide bridges in the protein, as isolated. ..
Hensley M, Gunasekera T, Easton J, Sigdel T, Sugarbaker S, Klingbeil L, et al
. Characterization of Zn(II)-responsive ribosomal proteins YkgM and L31 in E. coli. J Inorg Biochem. 2012;111:164-72 pubmed publisher
..These results are discussed in light of Zn(II) starvation model that has been postulated for B. subtilis...
Fanning T, Traut R. Topography of the C. coli 5S RNA-protein complex as determined by crosslinking with dimethyl suberimidate and dimethyl-3,3'-dithiobispropionimidate. Nucleic Acids Res. 1981;9:993-1004 pubmed
..No crosslinking was detected between L25 and the other three proteins. ..
Eistetter A, Butler P, Traut R, Fanning T. Characterization of Escherichia coli 50S ribosomal protein L31. FEMS Microbiol Lett. 1999;180:345-9 pubmed
Zhong H, Zhang Y, Wen Z, Li L. Protein sequencing by mass analysis of polypeptide ladders after controlled protein hydrolysis. Nat Biotechnol. 2004;22:1291-6 pubmed
..This technique can potentially be automated for large-scale protein annotation. ..
Lilleorg S, Reier K, Remme J, Liiv A. The Intersubunit Bridge B1b of the Bacterial Ribosome Facilitates Initiation of Protein Synthesis and Maintenance of Translational Fidelity. J Mol Biol. 2017;429:1067-1080 pubmed publisher
..We conclude that the only protein-protein intersubunit bridge of the bacterial ribosome facilitates translation initiation and is essential for maintaining the reading frame of mRNA translation. ..
Graifer D, Babkina G, Matasova N, Vladimirov S, Karpova G, Vlassov V. Structural arrangement of tRNA binding sites on Escherichia coli ribosomes, as revealed from data on affinity labelling with photoactivatable tRNA derivatives. Biochim Biophys Acta. 1989;1008:146-56 pubmed
Dabbs E. The gene for ribosomal protein L31, rpmE, is located at 88.5 minutes on the Escherichia coli chromosomal linkage map. J Bacteriol. 1981;148:379-82 pubmed
..5 min on the Escherichia coli chromosomal linkage map. They were located between metB and argH and cotransduced over 90% with metB. These mutations were shown to define the structural gene of protein L31, rpmE.
Hoving S, Munchbach M, Schmid H, Signor L, Lehmann A, Staudenmann W, et al
. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal Chem. 2000;72:1006-14 pubmed
..We show that chemical tagging is a viable first-pass high-throughput identification method to be used prior to an in depth MS/MS analysis. ..