Gene Symbol: rpmD
Description: 50S ribosomal subunit protein L30
Alias: ECK3289, JW3264
Species: Escherichia coli str. K-12 substr. MG1655
Geyl D, Bock A, Wittmann H. Cold-sensitive growth of a mutant of Escherichia coli with an altered ribosomal protein S8: analysis of revertants. Mol Gen Genet. 1977;152:331-6 pubmed
Dabbs E. Selection for Escherichia coli mutants with proteins missing from the ribosome. J Bacteriol. 1979;140:734-7 pubmed
..Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing. ..
Ritter E, Wittmann Liebold B. The primary structure of protein L30 from Escherichia coli ribosomes. FEBS Lett. 1975;60:153-5 pubmed
Osswald M, Greuer B, Brimacombe R. Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents. Nucleic Acids Res. 1990;18:6755-60 pubmed
..Cross-links to 5S RNA were observed with L5 (positions 34-41), and L18 (precise site not localised). ..
Cerretti D, Dean D, Davis G, Bedwell D, Nomura M. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 1983;11:2599-616 pubmed
..proteins: L14, L24, L5, S14, S8, L6, L18, S5, L30 and L15 (rp1N, rp1X, rp1E, rpsN, rpsH, rp1F, rp1R, rpsE, rpmD, and rp1O). We now report the entire 5.9 kb nucleotide sequence of the spc operon...
Dabbs E, Hasenbank R, Kastner B, Rak K, Wartusch B, Stoffler G. Immunological studies of Escherichia coli mutants lacking one or two ribosomal proteins. Mol Gen Genet. 1983;192:301-8 pubmed
..In several mutants in which a protein was not missing but altered, it was present as several moieties of differing charge and size. ..
Hoving S, Munchbach M, Schmid H, Signor L, Lehmann A, Staudenmann W, et al
. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal Chem. 2000;72:1006-14 pubmed
..We show that chemical tagging is a viable first-pass high-throughput identification method to be used prior to an in depth MS/MS analysis. ..