Gene Symbol: rpmB
Description: 50S ribosomal subunit protein L28
Alias: ECK3627, JW3612
Species: Escherichia coli str. K-12 substr. MG1655
Products:     rpmB

Top Publications

  1. Dabbs E. Selection for Escherichia coli mutants with proteins missing from the ribosome. J Bacteriol. 1979;140:734-7 pubmed
    ..Mutants were found with protein S20, L11, L15, L28, L29, or L30 missing. ..
  2. Walleczek J, Redl B, Stoffler Meilicke M, Stoffler G. Protein-protein cross-linking of the 50 S ribosomal subunit of Escherichia coli using 2-iminothiolane. Identification of cross-links by immunoblotting techniques. J Biol Chem. 1989;264:4231-7 pubmed
  3. Isono K, Schnier J, Kitakawa M. Genetic fine structure of the pyrE region containing the genes for ribosomal proteins L28 and L33 in Escherichia coli. Mol Gen Genet. 1980;179:311-7 pubmed
    ..The genes affected by the mutations have been concluded to be the structural genes for proteins L28 (rpmB) and L33 (rpmG) by constructing merodiploids heterozygous for pyrE and for the two ribosomal proteins...
  4. Lee J, An G, Friesen J, Isono K. Cloning and the nucleotide sequence of the genes for Escherichia coli ribosomal proteins L28 (rpmB) and L33 (rpmG). Mol Gen Genet. 1981;184:218-23 pubmed
    ..transducing bacteriophage lambda dpyrE DNA was used as a source of DNA to clone two ribosomal protein genes rpmB (L28) and rpmG (L33) on the cloning vehicle pACYC184...
  5. Chistyakov P, Venjaminova A, Vladimirov S, Graifer D, Kazakov S, Karpova G. Differences in 23 S rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent. FEBS Lett. 1989;246:197-201 pubmed
    ..16 S rRNA was found to be cross-linked preferentially to a single protein, S1, in both states of the ribosomes. ..
  6. Osswald M, Greuer B, Brimacombe R. Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents. Nucleic Acids Res. 1990;18:6755-60 pubmed
    ..Cross-links to 5S RNA were observed with L5 (positions 34-41), and L18 (precise site not localised). ..
  7. Maguire B, Wild D. The roles of proteins L28 and L33 in the assembly and function of Escherichia coli ribosomes in vivo. Mol Microbiol. 1997;23:237-45 pubmed
    Strain BM108 of Escherichia coli has a chromosomal mutation in the rpmB,G operon that prevents synthesis of ribosomal proteins L28 and L33. The mutation was lethal unless synthesis of protein L28 was induced from a plasmid...
  8. Tejedor F, Ballesta J. Components of the macrolide binding site on the ribosome. J Antimicrob Chemother. 1985;16 Suppl A:53-62 pubmed
    ..Ribosomal protein L27 is the major labeled component, indicating that this polypeptide, which seems to be part of the peptidyl transferase centre of the ribosome, also plays an important role on the macrolide binding site. ..
  9. Maguire B, Wild D. Mutations in the rpmBG operon of Escherichia coli that affect ribosome assembly. J Bacteriol. 1997;179:2486-93 pubmed
    ..In the new backgrounds, the mutation in rpmB was complemented by synthesis of protein L28 from a plasmid; the rpmG mutation was not complemented by protein L33 ..

More Information


  1. Maguire B, Wild D. The effects of mutations in the rpmB,G operon of Escherichia coli on ribosome assembly and ribosomal protein synthesis. Biochim Biophys Acta. 1997;1353:137-47 pubmed
    The rpmB,G operon of Escherichia coli codes for proteins L28 and L33 of the larger (50S) ribosomal subunit. Strains with mutations in this operon can help define the roles of these proteins in ribosome synthesis and function...
  2. Wittmann Liebold B, Marzinzig E. Primary structure of protein L28 from the large subunit of Escherichia coli ribosomes. FEBS Lett. 1977;81:214-7 pubmed
  3. Urlaub H, Kruft V, Bischof O, Müller E, Wittmann Liebold B. Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies. EMBO J. 1995;14:4578-88 pubmed
  4. Zheng C, Yang L, Hoopmann M, Eng J, Tang X, Weisbrod C, et al. Cross-linking measurements of in vivo protein complex topologies. Mol Cell Proteomics. 2011;10:M110.006841 pubmed publisher
    ..Furthermore, our unbiased data provide novel in vivo topological information that can impact understanding of biological function, even for cases where high resolution structures are not yet available. ..
  5. Hoving S, Munchbach M, Schmid H, Signor L, Lehmann A, Staudenmann W, et al. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal Chem. 2000;72:1006-14 pubmed
    ..We show that chemical tagging is a viable first-pass high-throughput identification method to be used prior to an in depth MS/MS analysis. ..
  6. Sköld S. RNA-protein complexes identified by crosslinking of polysomes. Biochimie. 1981;63:53-60 pubmed
    ..Additional crosslinked complexes were observed between 16S RNA and S1, S2 as well as S6 from the 30S subunit; and between 23S RNA and L10, L11, L7/12 from the 50S subunit. ..
  7. Redl B, Walleczek J, Stoffler Meilicke M, Stoffler G. Immunoblotting analysis of protein-protein crosslinks within the 50S ribosomal subunit of Escherichia coli. A study using dimethylsuberimidate as crosslinking reagent. Eur J Biochem. 1989;181:351-6 pubmed
    ..We can now propose the approximate locations of ten proteins that have not previously been localized. ..
  8. Spitzer E, Weiss B. dfp Gene of Escherichia coli K-12, a locus affecting DNA synthesis, codes for a flavoprotein. J Bacteriol. 1985;164:994-1003 pubmed
    ..dfp is located between the rpmB and dut genes at 81 min on the linkage map of Escherichia coli K-12...
  9. Zhao Q, Ofverstedt L, Skoglund U, Isaksson L. Morphological variation of individual Escherichia coli 50S ribosomal subunits in situ, as revealed by cryo-electron tomography. Exp Cell Res. 2004;300:190-201 pubmed
    ..In addition, gross morphological heterogeneity was observed in the reconstructions. Our results demonstrate a considerable structural variability among individual 50S subunits in the intracellular environment. ..
  10. Gifford C, Wallace S. The genes encoding formamidopyrimidine and MutY DNA glycosylases in Escherichia coli are transcribed as part of complex operons. J Bacteriol. 1999;181:4223-36 pubmed
    ..fpg is the terminal gene in an operon with the gene order radC, rpmB, rpmG, and fpg...
  11. Walleczek J, Martin T, Redl B, Stoffler Meilicke M, Stoffler G. Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli. Biochemistry. 1989;28:4099-105 pubmed
    ..Incorporation of our cross-linking results into the three-dimensional model of the 50S ribosomal subunit derived from immunoelectron microscopy yields the locations for 29 of the 33 proteins within the larger ribosomal subunit. ..
  12. Dabbs E, Hasenbank R, Kastner B, Rak K, Wartusch B, Stoffler G. Immunological studies of Escherichia coli mutants lacking one or two ribosomal proteins. Mol Gen Genet. 1983;192:301-8 pubmed
    ..In several mutants in which a protein was not missing but altered, it was present as several moieties of differing charge and size. ..