Gene Symbol: rplM
Description: 50S ribosomal subunit protein L13
Alias: ECK3220, JW3200
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Graifer D, Babkina G, Matasova N, Vladimirov S, Karpova G, Vlassov V. Structural arrangement of tRNA binding sites on Escherichia coli ribosomes, as revealed from data on affinity labelling with photoactivatable tRNA derivatives. Biochim Biophys Acta. 1989;1008:146-56 pubmed
  2. Walleczek J, Martin T, Redl B, Stoffler Meilicke M, Stoffler G. Comparative cross-linking study on the 50S ribosomal subunit from Escherichia coli. Biochemistry. 1989;28:4099-105 pubmed
    ..Incorporation of our cross-linking results into the three-dimensional model of the 50S ribosomal subunit derived from immunoelectron microscopy yields the locations for 29 of the 33 proteins within the larger ribosomal subunit. ..
  3. Spierer P, Zimmerman R, Mackie G. RNA-protein interactions in the ribosome. Binding of 50-S-subunit proteins to 5' and 3' terminal segments of the 23-S RNA. Eur J Biochem. 1975;52:459-68 pubmed
    ..It was determined that the 5' terminal segment contains the specific binding sites for proteins L4, L20 and L24, whereas the 3' terminal segment contains the specific binding sites for proteins L1, L2, L3, L6, L13, L13 and L23. ..
  4. Semrad K, Green R, Schroeder R. RNA chaperone activity of large ribosomal subunit proteins from Escherichia coli. RNA. 2004;10:1855-60 pubmed
    ..Nearly a third of the 34 large ribosomal subunit proteins displayed RNA chaperone activity. We discuss a possible role of this function during ribosome assembly and during translation. ..
  5. Tangy F, Capmau M, Le Goffic F. Photo-induced labelling of Escherichia coli ribosomes by a tobramycin analog. Eur J Biochem. 1983;131:581-7 pubmed
    ..The 30-S subunit is, on the whole, labelled more efficiently than the 50-S subunit. ..
  6. Chistyakov P, Venjaminova A, Vladimirov S, Graifer D, Kazakov S, Karpova G. Differences in 23 S rRNA-protein neighbourhood in Escherichia coli 70 S ribosomes and 70 S initiation complex. Probing by bifunctional Pt(II)-containing reagent. FEBS Lett. 1989;246:197-201 pubmed
    ..16 S rRNA was found to be cross-linked preferentially to a single protein, S1, in both states of the ribosomes. ..
  7. Isono S, Thamm S, Kitakawa M, Isono K. Cloning and nucleotide sequencing of the genes for ribosomal proteins S9 (rpsI) and L13 (rplM) of Escherichia coli. Mol Gen Genet. 1985;198:279-82 pubmed
    The genes for the ribosomal proteins S9 (rpsI) and L13 (rplM) of Escherichia coli have been cloned into a lambda phage vector termed L47.1...
  8. Charollais J, Pflieger D, Vinh J, Dreyfus M, Iost I. The DEAD-box RNA helicase SrmB is involved in the assembly of 50S ribosomal subunits in Escherichia coli. Mol Microbiol. 2003;48:1253-65 pubmed
    ..This step may consist of a structural rearrangement that, at low temperature, cannot occur without the assistance of this putative RNA helicase. ..
  9. Kärgel H, Stahl J, Gross B, Knespel S, Bielka H, Saarma M. Studies on interaction of 5 S RNA with ribosomal proteins. FEBS Lett. 1987;220:126-8 pubmed
    ..Under identical conditions, blots with proteins of the small ribosomal subunit (TP 40) did not bind 5 S RNA. ..

More Information


  1. Walleczek J, Redl B, Stoffler Meilicke M, Stoffler G. Protein-protein cross-linking of the 50 S ribosomal subunit of Escherichia coli using 2-iminothiolane. Identification of cross-links by immunoblotting techniques. J Biol Chem. 1989;264:4231-7 pubmed
  2. Muralikrishna P, Cooperman B. Ribosomal components neighboring the 2475 loop in Escherichia coli 50S subunits. Biochemistry. 1995;34:115-21 pubmed
    ..The implications of these results for the evolving model of the internal structure of the 50S subunit are considered. ..
  3. Tumminia S, Hellmann W, Wall J, Boublik M. Visualization of protein-nucleic acid interactions involved in the in vitro assembly of the Escherichia coli 50 S ribosomal subunit. J Mol Biol. 1994;235:1239-50 pubmed
    ..These mass centers merge into one central body, which is gradually enhanced and decorated with structural elements characteristic of the 50 S subunit in the latter stages of assembly. ..
  4. Schönfeld H, Foulaki K. Preoperative isolation of a reversible protein-protein crosslink generated in 50 S subunits of Escherichia coli ribosomes and identification of its components. Anal Biochem. 1987;164:23-30 pubmed
    ..Two-dimensional gel electrophoresis and immunological results established L13 and L21 as the components of this crosslink. ..
  5. Dabbs E. The gene for ribosomal protein L13, rplM, is located near argR, at about 70 minutes on the Escherichia coli chromosomal linkage map. J Bacteriol. 1982;149:779-82 pubmed
    ..of Escherichia coli with alterations in the electrophoretic mobility of ribosomal protein L13 were used to locate rplM, the gene for this protein, on the chromosomal linkage map...
  6. Redl B, Walleczek J, Stoffler Meilicke M, Stoffler G. Immunoblotting analysis of protein-protein crosslinks within the 50S ribosomal subunit of Escherichia coli. A study using dimethylsuberimidate as crosslinking reagent. Eur J Biochem. 1989;181:351-6 pubmed
    ..We can now propose the approximate locations of ten proteins that have not previously been localized. ..
  7. Bowen W, Van Dyke N, Murgola E, Lodmell J, Hill W. Interaction of thiostrepton and elongation factor-G with the ribosomal protein L11-binding domain. J Biol Chem. 2005;280:2934-43 pubmed
    ..The implications for recent models of thiostrepton activity and factor interactions are discussed. ..
  8. Barritault D, Expert Bezançon A, Milet M. [Nearest-neighbor relationships among 50S ribosomal proteins of E. coli]. C R Acad Sci Hebd Seances Acad Sci D. 1975;281:1043-6 pubmed
    ..The vicinity of these proteins in the ribosome is discussed. ..
  9. Osswald M, Greuer B, Brimacombe R. Localization of a series of RNA-protein cross-link sites in the 23S and 5S ribosomal RNA from Escherichia coli, induced by treatment of 50S subunits with three different bifunctional reagents. Nucleic Acids Res. 1990;18:6755-60 pubmed
    ..Cross-links to 5S RNA were observed with L5 (positions 34-41), and L18 (precise site not localised). ..
  10. Spierer P, Zimmermann R. RNA-protein interactions in the ribosome. Binding of proteins L1, L3, L6, L13 and L23 to specific fragments of the 23S RNA. FEBS Lett. 1976;68:71-5 pubmed
  11. Hoving S, Munchbach M, Schmid H, Signor L, Lehmann A, Staudenmann W, et al. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal Chem. 2000;72:1006-14 pubmed
    ..We show that chemical tagging is a viable first-pass high-throughput identification method to be used prior to an in depth MS/MS analysis. ..
  12. Tejedor F, Amils R, Ballesta J. Photoaffinity labeling of the pactamycin binding site on eubacterial ribosomes. Biochemistry. 1985;24:3667-72 pubmed
    ..The major labeled proteins are S2, S4, S18, S21, and L13. These proteins in the pactamycin binding site are probably related to the initiation step of protein synthesis. ..
  13. Sköld S. RNA-protein complexes identified by crosslinking of polysomes. Biochimie. 1981;63:53-60 pubmed
    ..Additional crosslinked complexes were observed between 16S RNA and S1, S2 as well as S6 from the 30S subunit; and between 23S RNA and L10, L11, L7/12 from the 50S subunit. ..
  14. Aseev L, Koledinskaya L, Boni I. Regulation of Ribosomal Protein Operons rplM-rpsI, rpmB-rpmG, and rplU-rpmA at the Transcriptional and Translational Levels. J Bacteriol. 2016;198:2494-502 pubmed publisher
    ..This paper describes for the first time the in vivo regulation of three r-protein operons, rplM-rpsI, rpmB-rpmG, and rplU-rpmA The results demonstrate that transcription of all three operons is subject to ppGpp/..
  15. Katayama A, Tsujii A, Wada A, Nishino T, Ishihama A. Systematic search for zinc-binding proteins in Escherichia coli. Eur J Biochem. 2002;269:2403-13 pubmed
    ..transketolase isozymes (TktA/TktB), translation elongation factor Ts (Tsf), ribosomal proteins L2 (RplB), L13 (RplM) and one of S15 (RpsO), S16 (RpsP) or S17 (RpsQ)...
  16. Mende L. The primary structure of protein L13 from the large subunit of Escherichia coli ribosomes. FEBS Lett. 1978;96:313-6 pubmed
  17. Yoshida H, Maki Y, Kato H, Fujisawa H, Izutsu K, Wada C, et al. The ribosome modulation factor (RMF) binding site on the 100S ribosome of Escherichia coli. J Biochem. 2002;132:983-9 pubmed
    ..We suggest that this conversion of 70S to 100S ribosomes represents a newly identified stage of the ribosomal cycle in stationary phase cells, and we have termed it the "Hibernation" stage. ..
  18. Kaczanowska M, Ryden Aulin M. Temperature sensitivity caused by mutant release factor 1 is suppressed by mutations that affect 16S rRNA maturation. J Bacteriol. 2004;186:3046-55 pubmed
    ..The first mutation disrupts a base pair in the transcription terminator stem for the rplM-rpsI operon, which encodes ribosomal proteins L13 and S9...
  19. Krassnigg F, Erdmann V, Fasold H. The synthesis of a photoreactive puromycin analogue and its application for labeling proteins in the 50-S subunit of Escherichia coli ribosomes. Eur J Biochem. 1978;87:439-43 pubmed
    ..On the basis of the affinity label used and supportive data from the literature, it is concluded that these proteins are at the active center of the 50-S particle and probably belong to the region of the ribosomal A site. ..