Gene Symbol: rplE
Description: 50S ribosomal subunit protein L5
Alias: ECK3295, JW3270
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Korepanov A, Gongadze G, Garber M, Court D, Bubunenko M. Importance of the 5 S rRNA-binding ribosomal proteins for cell viability and translation in Escherichia coli. J Mol Biol. 2007;366:1199-208 pubmed
    ..Here we studied the importance of Escherichia coli genes rplE, rplR and rplY, encoding 5 S rRNA-binding ribosomal proteins L5, L18 and L25, respectively, for cell growth, ..
  2. Gao H, Sengupta J, Valle M, Korostelev A, Eswar N, Stagg S, et al. Study of the structural dynamics of the E coli 70S ribosome using real-space refinement. Cell. 2003;113:789-801 pubmed
  3. Spierer P, Zimmermann R. Stoichiometry, cooperativity, and stability of interactions between 5S RNA and proteins L5, L18, and L25 from the 50S ribosomal subunit of Escherichia coli. Biochemistry. 1978;17:2474-9 pubmed
  4. Liou Y, Yoshikawa M, Tanaka N. Alteration of ribosomal protein L5 in a thiopeptin-resistant mutant of Escherichia coli. Biochem Biophys Res Commun. 1975;65:1096-101 pubmed
  5. Pye D, Barber J. Co-operative binding of ribosomal proteins to an erythromycin affinity column. Biochem Int. 1990;21:909-14 pubmed
    ..The binding of the E. coli ribosomal proteins L4, L5 and L21 to an erythromycin affinity column has been found to be co-operative. Binding does not occur in the absence of other ribosomal proteins. ..
  6. Cerretti D, Dean D, Davis G, Bedwell D, Nomura M. The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene. Nucleic Acids Res. 1983;11:2599-616 pubmed
  7. Olins P, Nomura M. Translational regulation by ribosomal protein S8 in Escherichia coli: structural homology between rRNA binding site and feedback target on mRNA. Nucleic Acids Res. 1981;9:1757-64 pubmed
    ..These results support the theory that the regulation of ribosomal protein synthesis is based on competition between rRNA and mRNA for regulatory ribosomal proteins. ..
  8. Huber P, Wool I. Nuclease protection analysis of ribonucleoprotein complexes: use of the cytotoxic ribonuclease alpha-sarcin to determine the binding sites for Escherichia coli ribosomal proteins L5, L18, and L25 on 5S rRNA. Proc Natl Acad Sci U S A. 1984;81:322-6 pubmed
    ..The procedure should be useful for the analysis of other ribonucleoprotein complexes. ..
  9. Lambert J, Traut R. The subunit interface of the Escherichia coli ribosome. Identification of proteins at the interface between the 30 S and 50 S subunits by crosslinking with 2-iminothiolane. J Mol Biol. 1981;149:451-76 pubmed

More Information


  1. Osswald M, Doring T, Brimacombe R. The ribosomal neighbourhood of the central fold of tRNA: cross-links from position 47 of tRNA located at the A, P or E site. Nucleic Acids Res. 1995;23:4635-41 pubmed
    ..In most (but not all) cases, more precise localizations of the cross-link sites could be made by primer extension analysis. ..
  2. Hoving S, Munchbach M, Schmid H, Signor L, Lehmann A, Staudenmann W, et al. A method for the chemical generation of N-terminal peptide sequence tags for rapid protein identification. Anal Chem. 2000;72:1006-14 pubmed
    ..We show that chemical tagging is a viable first-pass high-throughput identification method to be used prior to an in depth MS/MS analysis. ..
  3. Campbell T, Brown E. Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis. J Bacteriol. 2008;190:2537-45 pubmed publisher
    ..Taken in context, our work is most consistent with the hypothesis that YjeQ has a role in late 30S subunit biogenesis. ..
  4. Chen R, Ehrke G. The primary structure of the 5 S RNA binding protein L5 of Escherichia coli ribosomes. FEBS Lett. 1976;69:240-5 pubmed
  5. Shpanchenko O, Zvereva M, Dontsova O, Nierhaus K, Bogdanov A. 5S rRNA sugar-phosphate backbone protection in complexes with specific ribosomal proteins. FEBS Lett. 1996;394:71-5 pubmed
    ..The protection pattern differs from that for individual proteins because of the possible rearrangement of the structure. ..
  6. Ostergaard P, Phan H, Johansen L, Egebjerg J, Ostergaard L, Porse B, et al. Assembly of proteins and 5 S rRNA to transcripts of the major structural domains of 23 S rRNA. J Mol Biol. 1998;284:227-40 pubmed
    ..A similar approach was used to map the putative binding regions on domain V of protein L9 and the 5 S RNA-L5-L18 complex. ..
  7. Korepanov A, Korobeinikova A, Shestakov S, Garber M, Gongadze G. Protein L5 is crucial for in vivo assembly of the bacterial 50S ribosomal subunit central protuberance. Nucleic Acids Res. 2012;40:9153-9 pubmed publisher
    ..A possible model for the CP assembly in vivo is discussed in view of the data obtained...