rodA

Summary

Gene Symbol: rodA
Description: cell wall shape-determining protein
Alias: ECK0627, JW0629, rodA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Fange D, Elf J. Noise-induced Min phenotypes in E. coli. PLoS Comput Biol. 2006;2:e80 pubmed
    ..model, but that a stochastic model is necessary to account for several of the characteristics of the spherical (rodA-) and phospathedylethanolamide-deficient (PE-) phenotypes...
  2. Vats P, Shih Y, Rothfield L. Assembly of the MreB-associated cytoskeletal ring of Escherichia coli. Mol Microbiol. 2009;72:170-82 pubmed publisher
    ..We show here that the MreB cytoskeletal ring also contains the MreC, MreD, Pbp2 and RodA proteins...
  3. Corbin B, Yu X, Margolin W. Exploring intracellular space: function of the Min system in round-shaped Escherichia coli. EMBO J. 2002;21:1998-2008 pubmed publisher
    ..system might function in cells with multiple potential division planes, we investigated its role in a round-cell rodA mutant...
  4. Holtje J. Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli. Microbiol Mol Biol Rev. 1998;62:181-203 pubmed
    ..A model is presented that postulates that maintenance of bacterial shape is achieved by the enzyme complex copying the preexisting murein sacculus that plays the role of a template. ..
  5. Vinella D, Joseleau Petit D, Thevenet D, Bouloc P, D Ari R. Penicillin-binding protein 2 inactivation in Escherichia coli results in cell division inhibition, which is relieved by FtsZ overexpression. J Bacteriol. 1993;175:6704-10 pubmed
    ..We constructed a leuS(Ts) delta (rodA-pbpA)::Kmr strain, in which the PBP2 structural gene is deleted...
  6. Woods R, Schneider D, Winkworth C, Riley M, Lenski R. Tests of parallel molecular evolution in a long-term experiment with Escherichia coli. Proc Natl Acad Sci U S A. 2006;103:9107-12 pubmed publisher
    ..candidate genes were identified based on parallel phenotypic changes, here we sequenced four loci (pykF, nadR, pbpA-rodA, and hokB/sokB) in which mutations of unknown effect had been discovered in one population, and then we compared ..
  7. Begg K, Donachie W. Division planes alternate in spherical cells of Escherichia coli. J Bacteriol. 1998;180:2564-7 pubmed
    In the spherical cells of Escherichia coli rodA mutants, division is initiated at a single point, from which a furrow extends progressively around the cell...
  8. de Pedro M, Donachie W, Holtje J, Schwarz H. Constitutive septal murein synthesis in Escherichia coli with impaired activity of the morphogenetic proteins RodA and penicillin-binding protein 2. J Bacteriol. 2001;183:4115-26 pubmed
    ..in cells of Escherichia coli with impaired activity of the morphogenetic proteins penicillin-binding protein 2 and RodA has been investigated by the D-cysteine-biotin immunolabeling technique (M. A. de Pedro, J. C. Quintela, J.-V...
  9. Begg K, Donachie W. Cell shape and division in Escherichia coli: experiments with shape and division mutants. J Bacteriol. 1985;163:615-22 pubmed
    Double mutants which carry mutations in genes (rodA, pbpA) required for cell elongation (i.e., maintenance of rod shape) in combination with mutations in genes (ftsA, ftsI, ftsQ, or ftsZ) required for septation were constructed...

More Information

Publications36

  1. BendezĂș F, de Boer P. Conditional lethality, division defects, membrane involution, and endocytosis in mre and mrd shape mutants of Escherichia coli. J Bacteriol. 2008;190:1792-811 pubmed
    Maintenance of rod shape in Escherichia coli requires the shape proteins MreB, MreC, MreD, MrdA (PBP2), and MrdB (RodA). How loss of the Mre proteins affects E. coli viability has been unclear...
  2. Meeske A, Riley E, Robins W, Uehara T, Mekalanos J, Kahne D, et al. SEDS proteins are a widespread family of bacterial cell wall polymerases. Nature. 2016;537:634-638 pubmed publisher
    ..Cells lacking these enzymes survive by inducing an envelope stress response that increases the expression of RodA, a widely conserved core component of the Rod complex...
  3. Suzuki H, Nishimura Y, Hirota Y. On the process of cellular division in Escherichia coli: a series of mutants of E. coli altered in the penicillin-binding proteins. Proc Natl Acad Sci U S A. 1978;75:664-8 pubmed
    ..With the series of mutants, the chromosomal locus of the gene for each PBC was mapped: ponA (PBC-1a) 73.5 min, ponB (PBC-1b) 3.3 min, rodA (PBC-2) 14.4 min, ftsI (PBC-3) 1.8 min, dacB (PBC-4) 68 min, and dacA (PBC-5/6) 13.7 min.
  4. Ishino F, Park W, Tomioka S, Tamaki S, Takase I, Kunugita K, et al. Peptidoglycan synthetic activities in membranes of Escherichia coli caused by overproduction of penicillin-binding protein 2 and rodA protein. J Biol Chem. 1986;261:7024-31 pubmed
    Penicillin-binding protein (PBP)-2 and the RodA protein are known to function in determining the rod shape of Escherichia coli cells...
  5. Matsuzawa H, Asoh S, Kunai K, Muraiso K, Takasuga A, Ohta T. Nucleotide sequence of the rodA gene, responsible for the rod shape of Escherichia coli: rodA and the pbpA gene, encoding penicillin-binding protein 2, constitute the rodA operon. J Bacteriol. 1989;171:558-60 pubmed
    The rodA gene, which is responsible for the rod shape of Escherichia coli, was located 5 nucleotides downstream of another rod-shape-determining gene, pbpA, encoding penicillin-binding protein 2...
  6. Matsuzawa H, Hayakawa K, Sato T, Imahori K. Characterization and genetic analysis of a mutant of Escherichia coli K-12 with rounded morphology. J Bacteriol. 1973;115:436-42 pubmed
    ..The gene concerned, designated rodA, has been shown to be on the chromosome between the purE and pyrC loci and to be located at about minute 15...
  7. Asoh S, Matsuzawa H, Matsuhashi M, Ohta T. Molecular cloning and characterization of the genes (pbpA and rodA) responsible for the rod shape of Escherichia coli K-12: analysis of gene expression with transposon Tn5 mutagenesis and protein synthesis directed by constructed plasmids. J Bacteriol. 1983;154:10-6 pubmed
    ..shape-determining genes of Escherichia coli K-12, pbpA, the structural gene for penicillin-binding protein 2, and rodA, whose protein is unknown, were subcloned into plasmid vectors from the transducing phage lambda MAd lip24, which ..
  8. Philippe N, Pelosi L, Lenski R, Schneider D. Evolution of penicillin-binding protein 2 concentration and cell shape during a long-term experiment with Escherichia coli. J Bacteriol. 2009;191:909-21 pubmed publisher
    ..previously identified mutations in the pbpA operon in one-half of these populations; this operon encodes PBP2 and RodA proteins that are involved in cell wall elongation...
  9. Vinella D, D Ari R, Jaffe A, Bouloc P. Penicillin binding protein 2 is dispensable in Escherichia coli when ppGpp synthesis is induced. EMBO J. 1992;11:1493-501 pubmed
    ..We conclude that ppGpp regulates the transcription of a gene whose product is involved in mecillinam sensitivity, possibly as part of a chain of interacting elements which coordinate ribosomal activity with that of the PBPs. ..
  10. Iwaya M, Goldman R, Tipper D, Feingold B, Strominger J. Morphology of an Escherichia coli mutant with a temperature-dependent round cell shape. J Bacteriol. 1978;136:1143-58 pubmed
    ..Thus the mutation [rodA(Ts)] is most likely in the gene for penicillin-binding protein 2 and causes the organism to grow as a sphere at 42 ..
  11. Cho H, Wivagg C, Kapoor M, Barry Z, Rohs P, Suh H, et al. Bacterial cell wall biogenesis is mediated by SEDS and PBP polymerase families functioning semi-autonomously. Nat Microbiol. 2016;:16172 pubmed publisher
    ..These findings thus necessitate a fundamental change in our conception of the cell wall assembly process in bacteria. ..
  12. Bernhardt T, de Boer P. Screening for synthetic lethal mutants in Escherichia coli and identification of EnvC (YibP) as a periplasmic septal ring factor with murein hydrolase activity. Mol Microbiol. 2004;52:1255-69 pubmed
    ..By uncovering genetic interactions, the synthetic lethal screen described here provides an attractive new tool for studying gene function in E. coli. ..
  13. Errington J, Daniel R, Scheffers D. Cytokinesis in bacteria. Microbiol Mol Biol Rev. 2003;67:52-65, table of contents pubmed
    ..The functions of most of these proteins are unclear with the exception of at least one penicillin-binding protein, which catalyzes a key step in cell wall synthesis in the division septum. ..
  14. Spratt B, Boyd A, Stoker N. Defective and plaque-forming lambda transducing bacteriophage carrying penicillin-binding protein-cell shape genes: genetic and physical mapping and identification of gene products from the lip-dacA-rodA-pbpA-leuS region of the Escherichia coli chrom. J Bacteriol. 1980;143:569-81 pubmed
    ..The phage defined the gene order as lac---lip-dacA-rodA-pbpA-leuS---gal...
  15. Asoh S, Matsuzawa H, Ishino F, Strominger J, Matsuhashi M, Ohta T. Nucleotide sequence of the pbpA gene and characteristics of the deduced amino acid sequence of penicillin-binding protein 2 of Escherichia coli K12. Eur J Biochem. 1986;160:231-8 pubmed
    ..In the higher-molecular-mass PBPs 1A, 1B, 2 and 3, Ser-Xaa-Xaa-Lys-Pro was conserved. In the putative peptidoglycan transpeptidase domain there were six amino acid residues, which are common only in the PBPs of higher molecular mass. ..
  16. Takase I, Ishino F, Wachi M, Kamata H, Doi M, Asoh S, et al. Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome. J Bacteriol. 1987;169:5692-9 pubmed
    ..These two lipoproteins may be important in duplication of the cells. ..
  17. Bylund J, Haines M, Walsh K, Bouloc P, D Ari R, Higgins M. Buoyant density studies of several mecillinam-resistant and division mutants of Escherichia coli. J Bacteriol. 1991;173:5396-402 pubmed
    ..similar to that of lov mutant cells was observed in cells carrying the mecillinam-resistant mutations pbpA(Ts) and rodA(Ts) and the division mutation ftsI(Ts) at nonpermissive temperatures as well as in wild-type cells treated with ..
  18. Stoker N, Pratt J, Spratt B. Identification of the rodA gene product of Escherichia coli. J Bacteriol. 1983;155:854-9 pubmed
    Plasmids that carry the Escherichia coli cell shape gene rodA directed the synthesis of a cytoplasmic membrane protein (Mr, 31,000 [31K protein] ) in minicells, maxicells, and an in vitro-coupled transcription-translation system...
  19. Stoker N, Broome Smith J, Edelman A, Spratt B. Organization and subcloning of the dacA-rodA-pbpA cluster of cell shape genes in Escherichia coli. J Bacteriol. 1983;155:847-53 pubmed
    ..pBS10 carries a small cluster of Escherichia coli penicillin-binding protein/cell shape genes, including pbpA, rodA, and dacA...
  20. Tamaki S, Matsuzawa H, Matsuhashi M. Cluster of mrdA and mrdB genes responsible for the rod shape and mecillinam sensitivity of Escherichia coli. J Bacteriol. 1980;141:52-7 pubmed
    ..P1 transduction suggested that the most plausible gene order is leuS-mrdA-mrdB-lip. The rodA mutation reported previously seems to be similar to the mrdB murations, but the identities of the two have not yet ..
  21. Begg K, Spratt B, Donachie W. Interaction between membrane proteins PBP3 and rodA is required for normal cell shape and division in Escherichia coli. J Bacteriol. 1986;167:1004-8 pubmed
    ..the combination of certain mutations in a division gene (ftsI) with a specific mutation in one of the shape genes (rodA) could produce cells with normal shape and division, although separately these mutations led to a loss of the ..
  22. Begg K, Takasuga A, Edwards D, Dewar S, Spratt B, Adachi H, et al. The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide. J Bacteriol. 1990;172:6697-703 pubmed
    The rodA(Sui) mutation allows cell division to take place at 42 degrees C in ftsI23 mutant cells, which produce a thermolabile penicillin-binding protein 3 (PBP3, the septation-specific peptidoglycan transpeptidase)...
  23. Young K. Why spherical Escherichia coli dies: the inside story. J Bacteriol. 2008;190:1497-8 pubmed publisher
  24. Joris B, Dive G, Henriques A, Piggot P, Ghuysen J. The life-cycle proteins RodA of Escherichia coli and SpoVE of Bacillus subtilis have very similar primary structures. Mol Microbiol. 1990;4:513-7 pubmed
    Comparison of the predicted amino acid sequence of the cell-cycle RodA protein with the National Research Foundation protein sequence database shows that the 370-amino-acid RodA, a protein that is essential for wall elongation in ..
  25. Garcia del Portillo F, de Pedro M. Penicillin-binding protein 2 is essential for the integrity of growing cells of Escherichia coli ponB strains. J Bacteriol. 1991;173:4530-2 pubmed
    Analysis of Escherichia coli pbpA(Ts) or rodA(Ts) strains defective for penicillin-binding protein (PBP) 1A or PBP 1B indicated that the activity of PBP 2 is essential to prevent cell lysis in PBP 1B(-) strains and suggested that PBP 2 is ..
  26. Shiomi D, Toyoda A, Aizu T, Ejima F, Fujiyama A, Shini T, et al. Mutations in cell elongation genes mreB, mrdA and mrdB suppress the shape defect of RodZ-deficient cells. Mol Microbiol. 2013;87:1029-44 pubmed publisher
    ..In addition, suppressor mutations were found in the periplasmic regions in PBP2 and RodA, encoded by mrdA and mrdB genes...
  27. Iwaya M, Jones C, Khorana J, Strominger J. Mapping of the mecillinam-resistant, round morphological mutants of Escherichia coli. J Bacteriol. 1978;133:196-202 pubmed
    ..These are either the same or very close to the loci reported, respectively, for the rodA (H. Matsuzawa, K. Hayakawa, T. Sato, and K. Imahori, J. Bacteriol. 115:436-442, 1973) and envB genes (B...