Gene Symbol: rnlA
Description: CP4-57 prophage; RNase LS
Alias: ECK2626, JW2611, std, yfjN
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Kanesaki T, Hamada T, Yonesaki T. Opposite roles of the dmd gene in the control of RNase E and RNase LS activities. Genes Genet Syst. 2005;80:241-9 pubmed
    ..We found that RNase E was involved in the degradation of uvsY mRNA. Judging from the processing of gene-32 mRNA, RNase E activity declines after the beginning of the middle stage when dmd is defective. ..
  2. Naka K, Qi D, Yonesaki T, Otsuka Y. RnlB Antitoxin of the Escherichia coli RnlA-RnlB Toxin-Antitoxin Module Requires RNase HI for Inhibition of RnlA Toxin Activity. Toxins (Basel). 2017;9: pubmed publisher
    The Escherichia coli RnlA-RnlB toxin-antitoxin system is related to the anti-phage mechanism. Under normal growth conditions, an RnlA toxin with endoribonuclease activity is inhibited by binding of its cognate RnlB antitoxin...
  3. Otsuka Y, Ueno H, Yonesaki T. Escherichia coli endoribonucleases involved in cleavage of bacteriophage T4 mRNAs. J Bacteriol. 2003;185:983-90 pubmed
    ..In addition, we present evidence that 5'-truncated soc RNA, a stable form in T4-infected cells regardless of the presence of a dmd mutation, is generated by RNase E. ..
  4. Otsuka Y, Yonesaki T. A novel endoribonuclease, RNase LS, in Escherichia coli. Genetics. 2005;169:13-20 pubmed
    ..In this study, we demonstrated that rnlA (formerly yfjN) is essential for RNase LS activity both in vivo and in vitro...
  5. Yamanishi H, Yonesaki T. RNA cleavage linked with ribosomal action. Genetics. 2005;171:419-25 pubmed
    ..This effect of the ribosome could reflect remodeling of the high-order structure of the mRNA molecule. ..
  6. Otsuka Y, Yonesaki T. Dmd of bacteriophage T4 functions as an antitoxin against Escherichia coli LsoA and RnlA toxins. Mol Microbiol. 2012;83:669-81 pubmed
    ..Predicted proteins encoded by these two ORFs were found to share a weak homology with RnlA and RnlB, respectively, a toxin–antitoxin system encoded on the E. coli K-12 chromosome...
  7. Jain C. Degradation of mRNA in Escherichia coli. IUBMB Life. 2002;54:315-21 pubmed
    ..At the conclusion of this review, some of the anticipated directions of future research on this topic will be discussed. ..
  8. Otsuka Y, Koga M, Iwamoto A, Yonesaki T. A role of RnlA in the RNase LS activity from Escherichia coli. Genes Genet Syst. 2007;82:291-9 pubmed
    ..Our previous work demonstrated that E. coli rnlA is essential for RNase LS activity...
  9. Iwamoto A, Lemire S, Yonesaki T. Post-transcriptional control of Crp-cAMP by RNase LS in Escherichia coli. Mol Microbiol. 2008;70:1570-8 pubmed publisher
    Escherichia coli ribonuclease LS was first characterized as a potential antagonist of bacteriophage T4; the E. coli rnlA gene is required for this activity...

More Information


  1. Wei Y, Gao Z, Otsuka Y, Naka K, Yonesaki T, Zhang H, et al. Structure-function studies of Escherichia coli RnlA reveal a novel toxin structure involved in bacteriophage resistance. Mol Microbiol. 2013;90:956-65 pubmed publisher
    Escherichia coli RnlA-RnlB is a newly identified toxin-antitoxin (TA) system that plays a role in bacteriophage resistance...
  2. Naka K, Koga M, Yonesaki T, Otsuka Y. RNase HI stimulates the activity of RnlA toxin in Escherichia coli. Mol Microbiol. 2014;91:596-605 pubmed publisher
    A type II toxin-antitoxin system in Escherichia?coli, rnlA-rnlB, functions as an anti-phage mechanism. RnlA is a toxin with an endoribonuclease activity and the cognate RnlB inhibits RnlA toxicity in E.?coli cells...
  3. Koga M, Otsuka Y, Lemire S, Yonesaki T. Escherichia coli rnlA and rnlB compose a novel toxin-antitoxin system. Genetics. 2011;187:123-30 pubmed publisher
    ..Here we show that rnlA, a structural gene of RNase LS, encodes a novel toxin, and that rnlB (formally yfjO), located immediately ..