rng

Summary

Gene Symbol: rng
Description: ribonuclease G
Alias: ECK3235, JW3216, adhR, cafA, yhdF
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Li Z, Pandit S, Deutscher M. RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA. EMBO J. 1999;18:2878-85 pubmed
    ..Moreover, in the absence of CafA protein, a homolog of RNase E, formation of 16S RNA also slows down, but in this case a 16...
  2. Cairrao F, Arraiano C. The role of endoribonucleases in the regulation of RNase R. Biochem Biophys Res Commun. 2006;343:731-7 pubmed
    ..However, the accumulation of intermediary transcripts in an rng mutant suggests that RNase G may act in the degradation of the transcripts already cleaved by RNase E...
  3. Ow M, Perwez T, Kushner S. RNase G of Escherichia coli exhibits only limited functional overlap with its essential homologue, RNase E. Mol Microbiol. 2003;49:607-22 pubmed
    RNase G (rng) is an E. coli endoribonuclease that is homologous to the catalytic domain of RNase E (rne), an essential protein that is a major participant in tRNA maturation, mRNA decay, rRNA processing and M1 RNA processing...
  4. McDowall K, Hernandez R, Lin Chao S, Cohen S. The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus. J Bacteriol. 1993;175:4245-9 pubmed
    ..coli, which has been implicated previously in determining bacterial cell shape. ..
  5. Wachi M, Umitsuki G, Shimizu M, Takada A, Nagai K. Escherichia coli cafA gene encodes a novel RNase, designated as RNase G, involved in processing of the 5' end of 16S rRNA. Biochem Biophys Res Commun. 1999;259:483-8 pubmed
    We found that the Escherichia coli cafA::cat mutant accumulated a precursor of 16S rRNA. This precursor migrated to the same position with 16...
  6. Briant D, Hankins J, Cook M, Mackie G. The quaternary structure of RNase G from Escherichia coli. Mol Microbiol. 2003;50:1381-90 pubmed
    ..We propose that many of the cysteines in RNase G lie on its surface and define, in part, the subunit-subunit interface. ..
  7. Lee K, Bernstein J, Cohen S. RNase G complementation of rne null mutation identifies functional interrelationships with RNase E in Escherichia coli. Mol Microbiol. 2002;43:1445-56 pubmed
    The Escherichia coli endoribonucleases RNase E (Rne) and RNase G (Rng) have sequence similarity and broadly similar sequence specificity. Whereas the absence of Rne normally is lethal, we show here that E...
  8. Fang P, Wang J, Li X, Guo M, Xing L, Cao X, et al. Crystallization and preliminary X-ray analysis of Escherichia coli RNase G. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009;65:586-8 pubmed publisher
    ..Purified recombinant E. coli RNase G, which has 497 amino acids, was crystallized in the cubic space group F432, with unit-cell parameters a = b = c = 219.84 A. X-ray diffraction data were collected to a resolution of 3.4 A. ..
  9. Sakai T, Nakamura N, Umitsuki G, Nagai K, Wachi M. Increased production of pyruvic acid by Escherichia coli RNase G mutants in combination with cra mutations. Appl Microbiol Biotechnol. 2007;76:183-92 pubmed
    ..In fact, the rng cra double mutant produced increased amount of pyruvate in the medium...

More Information

Publications40

  1. Marcaida M, DePristo M, Chandran V, Carpousis A, Luisi B. The RNA degradosome: life in the fast lane of adaptive molecular evolution. Trends Biochem Sci. 2006;31:359-65 pubmed
  2. Wachi M, Umitsuki G, Nagai K. Functional relationship between Escherichia coli RNase E and the CafA protein. Mol Gen Genet. 1997;253:515-9 pubmed
    We analyzed the functional relationship between the Escherichia coli RNase E and the CafA protein, which show extensive sequence similarity...
  3. Deana A, Belasco J. The function of RNase G in Escherichia coli is constrained by its amino and carboxyl termini. Mol Microbiol. 2004;51:1205-17 pubmed
    ..coli RNAs other than tRNA. ..
  4. Wachi M, Kaga N, Umitsuki G, Clark D, Nagai K. A novel RNase G mutant that is defective in degradation of adhE mRNA but proficient in the processing of 16S rRNA precursor. Biochem Biophys Res Commun. 2001;289:1301-6 pubmed
    Escherichia coli RNase G, encoded by the rng gene, is involved in both the processing of 16S rRNA precursor and the degradation of adhE mRNA. Consequently, defects in RNase G result in elevation of AdhE levels...
  5. Clark D, Rod M. Regulatory mutations that allow the growth of Escherichia coli on butanol as carbon source. J Mol Evol. 1987;25:151-8 pubmed
    ..These mutations were found to map far from the adhC locus at around 90 min. Such adhR mutants were unable to grow on acetate or ethanol in certain media unless supplemented with extra manganese...
  6. Zajanckauskaite A, Truncaite L, Strazdaite Zieliene Z, Nivinskas R. Involvement of the Escherichia coli endoribonucleases G and E in the secondary processing of RegB-cleaved transcripts of bacteriophage T4. Virology. 2008;375:342-53 pubmed publisher
    ..Using plasmid-phage systems we demonstrate that T4 infection favours cleavage by the host endoribonucleases at these sites...
  7. Inagawa T, Okamoto S, Wachi M, Ochi K. RNase ES of Streptomyces coelicolor A3(2) can complement the rne and rng mutations in Escherichia coli. Biosci Biotechnol Biochem. 2003;67:1767-71 pubmed
    ..We expressed S. coelicolor RNase ES as a 6 x His-tagged protein in an Escherichia coli mutant carrying a rng (which encodes RNase G) or a rne (which encodes RNase E) mutation to study whether S...
  8. Feng Y, Vickers T, Cohen S. The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection. Proc Natl Acad Sci U S A. 2002;99:14746-51 pubmed
  9. Kaga N, Umitsuki G, Clark D, Nagai K, Wachi M. Extensive overproduction of the AdhE protein by rng mutations depends on mutations in the cra gene or in the Cra-box of the adhE promoter. Biochem Biophys Res Commun. 2002;295:92-7 pubmed
    Escherichia coli RNase G encoded by the rng gene is involved in degradation of adhE mRNA. Overproduction of the AdhE protein by rng mutants was found to depend on the genetic background of strains derived from DC272 (adhC81) or MC1061...
  10. Jourdan S, Kime L, McDowall K. The sequence of sites recognised by a member of the RNase E/G family can control the maximal rate of cleavage, while a 5'-monophosphorylated end appears to function cooperatively in mediating RNA binding. Biochem Biophys Res Commun. 2010;391:879-83 pubmed publisher
    ..Our results are discussed in terms of recent X-ray crystal structures and models for the initiation of bacterial mRNA degradation. ..
  11. Jiang X, Diwa A, Belasco J. Regions of RNase E important for 5'-end-dependent RNA cleavage and autoregulated synthesis. J Bacteriol. 2000;182:2468-75 pubmed
    ..This property is shared by the related E. coli ribonuclease CafA (RNase G) and by a cyanobacterial RNase E homolog derived from Synechocystis, indicating that the 5'-end dependence ..
  12. Wachi M, Doi M, Ueda T, Ueki M, Tsuritani K, Nagai K, et al. Sequence of the downstream flanking region of the shape-determining genes mreBCD of Escherichia coli. Gene. 1991;106:135-6 pubmed
    ..Two open reading frames (ORFs) were found, a 591-bp orfE and a 1467-bp orfF. Based on the sequence, it is suggested that the three mre genes (encoding the murein pathway), mreB, mreC and mreD, and these two ORFs possibly form an operon. ..
  13. Deutscher M. Maturation and degradation of ribosomal RNA in bacteria. Prog Mol Biol Transl Sci. 2009;85:369-91 pubmed publisher
    ..The second focus of this review is to describe these degradative reactions, the RNases that carry them out, and the conditions that initiate the turnover process. ..
  14. Tock M, Walsh A, Carroll G, McDowall K. The CafA protein required for the 5'-maturation of 16 S rRNA is a 5'-end-dependent ribonuclease that has context-dependent broad sequence specificity. J Biol Chem. 2000;275:8726-32 pubmed
    The CafA protein, which was initially described as having a role in either Escherichia coli cell division or chromosomal segregation, has recently been shown to be required for the maturation of the 5'-end of 16 S rRNA...
  15. Carpousis A, Luisi B, McDowall K. Endonucleolytic initiation of mRNA decay in Escherichia coli. Prog Mol Biol Transl Sci. 2009;85:91-135 pubmed publisher
    ..Here, we discuss current models for mRNA degradation in E. coli and we present current thinking on the structure and function of RNase E based on recent crystal structures of its catalytic core. ..
  16. Tamura M, Lee K, Miller C, Moore C, Shirako Y, Kobayashi M, et al. RNase E maintenance of proper FtsZ/FtsA ratio required for nonfilamentous growth of Escherichia coli cells but not for colony-forming ability. J Bacteriol. 2006;188:5145-52 pubmed publisher
    ..Our results demonstrate that RNase E activity is required to maintain a proper cellular ratio of the FtsZ and FtsA proteins in E. coli but that FtsZ deficiency does not account for the nonviability of cells lacking RNase E...
  17. Carpousis A. The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E. Annu Rev Microbiol. 2007;61:71-87 pubmed
  18. Kime L, Jourdan S, McDowall K. Identifying and characterizing substrates of the RNase E/G family of enzymes. Methods Enzymol. 2008;447:215-41 pubmed publisher
    ..Most of the approaches that we describe can be applied to the study of homologs of E. coli RNase E, which have been found in approximately half of the eubacteria that have been sequenced. ..
  19. Okada Y, Shibata T, Matsuhashi M. Possible function of the cytoplasmic axial filaments in chromosomal segregation and cellular division of Escherichia coli. Sci Prog. 1993;77 ( Pt 3-4):253-64 pubmed
    Expression of the cafA gene at 71 min on the E...
  20. Chung D, Min Z, Wang B, Kushner S. Single amino acid changes in the predicted RNase H domain of Escherichia coli RNase G lead to complementation of RNase E deletion mutants. RNA. 2010;16:1371-85 pubmed publisher
    ..derived single amino acid substitutions within the predicted RNase H domain of RNase G, generating the rng-219 and rng-248 alleles, result in complementation of the growth defect associated with various RNase E mutants, ..
  21. Miczak A, Ford J, Marian M, Apirion D. RNA processing: new mutants that affect endonucleolytic processing of RNA. Biochem Biophys Res Commun. 1983;114:690-8 pubmed
    ..From one of the double mutants the rne mutation was removed, and the new single mutant, designated rng, was examined. In this mutant the maturation of host RNA as well as of bacteriophage T4 RNA is affected...
  22. Jourdan S, McDowall K. Sensing of 5' monophosphate by Escherichia coli RNase G can significantly enhance association with RNA and stimulate the decay of functional mRNA transcripts in vivo. Mol Microbiol. 2008;67:102-15 pubmed
  23. Kaga N, Umitsuki G, Nagai K, Wachi M. RNase G-dependent degradation of the eno mRNA encoding a glycolysis enzyme enolase in Escherichia coli. Biosci Biotechnol Biochem. 2002;66:2216-20 pubmed
    Escherichia coli RNase G, encoded by the rng gene, is involved in the processing of 16S rRNA and degradation of the adhE mRNA encoding a fermentative alcohol dehydrogenase...
  24. Okada Y, Wachi M, Hirata A, Suzuki K, Nagai K, Matsuhashi M. Cytoplasmic axial filaments in Escherichia coli cells: possible function in the mechanism of chromosome segregation and cell division. J Bacteriol. 1994;176:917-22 pubmed
    Overproduction of CafA caused formation of chained cells and minicells. The cafA gene is located downstream from the mre region at 71 min on the Escherichia coli chromosome map and was previously called orfF...
  25. Umitsuki G, Wachi M, Takada A, Hikichi T, Nagai K. Involvement of RNase G in in vivo mRNA metabolism in Escherichia coli. Genes Cells. 2001;6:403-10 pubmed
    Escherichia coli rng gene (previously called cafA) encodes a novel RNase, named RNase G, which is involved in the 5' end-processing of 16S rRNA. In rng mutant cells, a precursor form of 16S rRNA, 16.3S rRNA, is accumulated...
  26. Jain C. Degradation of mRNA in Escherichia coli. IUBMB Life. 2002;54:315-21 pubmed
    ..At the conclusion of this review, some of the anticipated directions of future research on this topic will be discussed. ..
  27. Mohanty B, Kushner S. Rho-independent transcription terminators inhibit RNase P processing of the secG leuU and metT tRNA polycistronic transcripts in Escherichia coli. Nucleic Acids Res. 2008;36:364-75 pubmed
    ..Taken together it is clear that there are multiple pathways involved in the maturation of tRNAs in E. coli. ..
  28. Viegas S, Pfeiffer V, Sittka A, Silva I, Vogel J, Arraiano C. Characterization of the role of ribonucleases in Salmonella small RNA decay. Nucleic Acids Res. 2007;35:7651-64 pubmed
    ..Taken together, the results of this study provide initial insight into the mechanisms of sRNA decay in Salmonella, and indicate specific contributions of the RNA decay machinery components to the turnover of individual sRNAs. ..
  29. Kim K, Lee Y. Regulation of 6S RNA biogenesis by switching utilization of both sigma factors and endoribonucleases. Nucleic Acids Res. 2004;32:6057-68 pubmed
    ..Our data indicate that the switching of the utilization of both sigma factors and endoribonucleases in the biogenesis of 6S RNA would play an essential role in modulating its levels in E.coli. ..
  30. Garrey S, Blech M, Riffell J, Hankins J, Stickney L, Diver M, et al. Substrate binding and active site residues in RNases E and G: role of the 5'-sensor. J Biol Chem. 2009;284:31843-50 pubmed publisher
    ..Our results point to the importance of alternative RNA binding sites in RNase E and to alternative pathways of RNA recognition. ..
  31. Jiang X, Belasco J. Catalytic activation of multimeric RNase E and RNase G by 5'-monophosphorylated RNA. Proc Natl Acad Sci U S A. 2004;101:9211-6 pubmed
    ..Among the molecular mechanisms that could account for these properties are those in which 5'-end binding by one enzyme subunit induces a protein structural change that accelerates RNA cleavage by another subunit. ..