rne

Summary

Gene Symbol: rne
Description: endoribonuclease; RNA-binding protein;RNA degradosome binding protein
Alias: ECK1069, JW1071, ams, hmp1, smbB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Afonyushkin T, Vecerek B, Moll I, Blasi U, Kaberdin V. Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB. Nucleic Acids Res. 2005;33:1678-89 pubmed
    ..These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover. ..
  2. Hajnsdorf E, Carpousis A, Regnier P. Nucleolytic inactivation and degradation of the RNase III processed pnp message encoding polynucleotide phosphorylase of Escherichia coli. J Mol Biol. 1994;239:439-54 pubmed
    ..Taken together, our results suggest that in wild-type E. coli the degradation of the RNase III processed mRNA is mediated by RNase E. ..
  3. Gao J, Lee K, Zhao M, Qiu J, Zhan X, Saxena A, et al. Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome. Mol Microbiol. 2006;61:394-406 pubmed
    ..coli. ..
  4. Moll I, Afonyushkin T, Vytvytska O, Kaberdin V, Bl si U. Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs. RNA. 2003;9:1308-14 pubmed
    ..It is commonly believed that the RNA chaperone Hfq facilitates or promotes the interaction between sRNAs and their mRNA targets. This study reveals another role for Hfq, that is, protection of sRNAs from endonucleolytic attack...
  5. Lin Chao S, Wei C, Lin Y. RNase E is required for the maturation of ssrA RNA and normal ssrA RNA peptide-tagging activity. Proc Natl Acad Sci U S A. 1999;96:12406-11 pubmed
    ..coli, also has the previously unsuspected ability to affect protein degradation through its role in maturation of the 3' end of ssrA RNA. ..
  6. Chandran V, Luisi B. Recognition of enolase in the Escherichia coli RNA degradosome. J Mol Biol. 2006;358:8-15 pubmed
    ..We suggest that enolase is recruited into putative RNA degradosome machinery in these bacilli, where it plays common regulatory functions. ..
  7. Aiba H. Mechanism of RNA silencing by Hfq-binding small RNAs. Curr Opin Microbiol. 2007;10:134-9 pubmed
    ..Hfq accelerates the rate of duplex formation between SgrS and the target mRNA. Membrane localization of target mRNA contributes to efficient SgrS action by competing with ribosome loading. ..
  8. Li Z, Pandit S, Deutscher M. RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA. EMBO J. 1999;18:2878-85 pubmed
    ..These data indicate that both RNase E and CafA protein are required for a two step, sequential maturation of the 5' end of 16S rRNA, and that CafA protein is a new ribonuclease. We propose that it be renamed RNase G. ..
  9. Khemici V, Carpousis A. The RNA degradosome and poly(A) polymerase of Escherichia coli are required in vivo for the degradation of small mRNA decay intermediates containing REP-stabilizers. Mol Microbiol. 2004;51:777-90 pubmed
  10. Lundberg U, Altman S. Processing of the precursor to the catalytic RNA subunit of RNase P from Escherichia coli. RNA. 1995;1:327-34 pubmed
    ..The enzymatic activity that removes the 5' nucleotides from the precursor to M1 RNA is not due to RNase E, RNase P, or RNase III alone. ..

Detail Information

Publications93

  1. Afonyushkin T, Vecerek B, Moll I, Blasi U, Kaberdin V. Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB. Nucleic Acids Res. 2005;33:1678-89 pubmed
    ..These data are discussed in terms of a model, which accounts for the observed roles of RNase E and RNase III in sodB mRNA turnover. ..
  2. Hajnsdorf E, Carpousis A, Regnier P. Nucleolytic inactivation and degradation of the RNase III processed pnp message encoding polynucleotide phosphorylase of Escherichia coli. J Mol Biol. 1994;239:439-54 pubmed
    ..Taken together, our results suggest that in wild-type E. coli the degradation of the RNase III processed mRNA is mediated by RNase E. ..
  3. Gao J, Lee K, Zhao M, Qiu J, Zhan X, Saxena A, et al. Differential modulation of E. coli mRNA abundance by inhibitory proteins that alter the composition of the degradosome. Mol Microbiol. 2006;61:394-406 pubmed
    ..coli. ..
  4. Moll I, Afonyushkin T, Vytvytska O, Kaberdin V, Bl si U. Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs. RNA. 2003;9:1308-14 pubmed
    ..It is commonly believed that the RNA chaperone Hfq facilitates or promotes the interaction between sRNAs and their mRNA targets. This study reveals another role for Hfq, that is, protection of sRNAs from endonucleolytic attack...
  5. Lin Chao S, Wei C, Lin Y. RNase E is required for the maturation of ssrA RNA and normal ssrA RNA peptide-tagging activity. Proc Natl Acad Sci U S A. 1999;96:12406-11 pubmed
    ..coli, also has the previously unsuspected ability to affect protein degradation through its role in maturation of the 3' end of ssrA RNA. ..
  6. Chandran V, Luisi B. Recognition of enolase in the Escherichia coli RNA degradosome. J Mol Biol. 2006;358:8-15 pubmed
    ..We suggest that enolase is recruited into putative RNA degradosome machinery in these bacilli, where it plays common regulatory functions. ..
  7. Aiba H. Mechanism of RNA silencing by Hfq-binding small RNAs. Curr Opin Microbiol. 2007;10:134-9 pubmed
    ..Hfq accelerates the rate of duplex formation between SgrS and the target mRNA. Membrane localization of target mRNA contributes to efficient SgrS action by competing with ribosome loading. ..
  8. Li Z, Pandit S, Deutscher M. RNase G (CafA protein) and RNase E are both required for the 5' maturation of 16S ribosomal RNA. EMBO J. 1999;18:2878-85 pubmed
    ..These data indicate that both RNase E and CafA protein are required for a two step, sequential maturation of the 5' end of 16S rRNA, and that CafA protein is a new ribonuclease. We propose that it be renamed RNase G. ..
  9. Khemici V, Carpousis A. The RNA degradosome and poly(A) polymerase of Escherichia coli are required in vivo for the degradation of small mRNA decay intermediates containing REP-stabilizers. Mol Microbiol. 2004;51:777-90 pubmed
  10. Lundberg U, Altman S. Processing of the precursor to the catalytic RNA subunit of RNase P from Escherichia coli. RNA. 1995;1:327-34 pubmed
    ..The enzymatic activity that removes the 5' nucleotides from the precursor to M1 RNA is not due to RNase E, RNase P, or RNase III alone. ..
  11. Lee K, Bernstein J, Cohen S. RNase G complementation of rne null mutation identifies functional interrelationships with RNase E in Escherichia coli. Mol Microbiol. 2002;43:1445-56 pubmed
    The Escherichia coli endoribonucleases RNase E (Rne) and RNase G (Rng) have sequence similarity and broadly similar sequence specificity. Whereas the absence of Rne normally is lethal, we show here that E...
  12. Callaghan A, Grossmann J, Redko Y, Ilag L, Moncrieffe M, Symmons M, et al. Quaternary structure and catalytic activity of the Escherichia coli ribonuclease E amino-terminal catalytic domain. Biochemistry. 2003;42:13848-55 pubmed
    ..These observations have implications for the structure and function of the RNase E/RNase G ribonuclease family and for the assembly of the E. coli RNA degradosome, in which RNase E is the central component. ..
  13. Marujo P, Braun F, Haugel Nielsen J, Le Derout J, Arraiano C, Regnier P. Inactivation of the decay pathway initiated at an internal site by RNase E promotes poly(A)-dependent degradation of the rpsO mRNA in Escherichia coli. Mol Microbiol. 2003;50:1283-94 pubmed
    ..We also discuss the role of poly(A)-dependent decay in mRNA metabolism. ..
  14. Mackie G. Stabilization of circular rpsT mRNA demonstrates the 5'-end dependence of RNase E action in vivo. J Biol Chem. 2000;275:25069-72 pubmed
  15. Miczak A, Srivastava R, Apirion D. Location of the RNA-processing enzymes RNase III, RNase E and RNase P in the Escherichia coli cell. Mol Microbiol. 1991;5:1801-10 pubmed
    ..The implications of these findings for the cellular organization of the RNA-processing enzymes in the cell are discussed. ..
  16. Vytvytska O, Moll I, Kaberdin V, von Gabain A, Blasi U. Hfq (HF1) stimulates ompA mRNA decay by interfering with ribosome binding. Genes Dev. 2000;14:1109-18 pubmed
    ..The data are discussed in terms of a model wherein Hfq regulates the stability of ompA mRNA by competing with 30S ribosomes for binding to the ompA 5'-UTR. ..
  17. Claverie Martin F, Diaz Torres M, Yancey S, Kushner S. Analysis of the altered mRNA stability (ams) gene from Escherichia coli. Nucleotide sequence, transcriptional analysis, and homology of its product to MRP3, a mitochondrial ribosomal protein from Neurospora crassa. J Biol Chem. 1991;266:2843-51 pubmed
    The product of the altered mRNA stability (ams) gene of Escherichia coli is involved in decay of mRNA. The complete nucleotide sequence of a 4-kilobase BamHI restriction fragment containing the ams coding sequence was determined...
  18. Ow M, Perwez T, Kushner S. RNase G of Escherichia coli exhibits only limited functional overlap with its essential homologue, RNase E. Mol Microbiol. 2003;49:607-22 pubmed
    RNase G (rng) is an E. coli endoribonuclease that is homologous to the catalytic domain of RNase E (rne), an essential protein that is a major participant in tRNA maturation, mRNA decay, rRNA processing and M1 RNA processing...
  19. Liou G, Jane W, Cohen S, Lin N, Lin Chao S. RNA degradosomes exist in vivo in Escherichia coli as multicomponent complexes associated with the cytoplasmic membrane via the N-terminal region of ribonuclease E. Proc Natl Acad Sci U S A. 2001;98:63-8 pubmed
    ..Our findings, which establish the existence and cellular location of RNase E-based degradosomes in vivo in E. coli, also suggest that RNA processing and decay may occur at specific sites within cells. ..
  20. Miczak A, Kaberdin V, Wei C, Lin Chao S. Proteins associated with RNase E in a multicomponent ribonucleolytic complex. Proc Natl Acad Sci U S A. 1996;93:3865-9 pubmed
    ..7.7.8). To isolate and identify other components of the RNase E complex, FLAG-epitope-tagged RNase E (FLAG-Rne) fusion protein was purified on a monoclonal antibody-conjugated agarose column...
  21. Morita T, Maki K, Aiba H. RNase E-based ribonucleoprotein complexes: mechanical basis of mRNA destabilization mediated by bacterial noncoding RNAs. Genes Dev. 2005;19:2176-86 pubmed
    ..The formation of ribonucleoprotein complexes containing RNases could be a general way by which small RNAs destabilize target mRNAs in both prokaryotes and eukaryotes. ..
  22. Cairrao F, Arraiano C. The role of endoribonucleases in the regulation of RNase R. Biochem Biophys Res Commun. 2006;343:731-7 pubmed
    ..These results demonstrated that other ribonucleases can act as an additional level of regulation in the control of the expression of RNase R. ..
  23. Coburn G, Miao X, Briant D, Mackie G. Reconstitution of a minimal RNA degradosome demonstrates functional coordination between a 3' exonuclease and a DEAD-box RNA helicase. Genes Dev. 1999;13:2594-603 pubmed
    ..The Rne protein serves as an essential scaffold in the reconstitution process; however, RNase E activity is not required...
  24. Carpousis A. The Escherichia coli RNA degradosome: structure, function and relationship in other ribonucleolytic multienzyme complexes. Biochem Soc Trans. 2002;30:150-5 pubmed
    ..RhlB in vitro can facilitate the degradation of structured RNA by PNPase. Since the discovery of the RNA degradosome in E. coli, related complexes have been described in other organisms. ..
  25. Jiang X, Diwa A, Belasco J. Regions of RNase E important for 5'-end-dependent RNA cleavage and autoregulated synthesis. J Bacteriol. 2000;182:2468-75 pubmed
    ..coli cell growth or in regulating RNase E production, even when overproduced sixfold relative to wild-type RNase E levels. ..
  26. Huang H, Liao J, Cohen S. Poly(A)- and poly(U)-specific RNA 3' tail shortening by E. coli ribonuclease E. Nature. 1998;391:99-102 pubmed
    ..The ability of RNase E to shorten poly(A) tails, together with the effect of tail length on endonucleolytic cleavage within primary transcripts, suggests a mechanism by which RNase E may exercise overall control over RNA decay. ..
  27. Garrey S, Blech M, Riffell J, Hankins J, Stickney L, Diver M, et al. Substrate binding and active site residues in RNases E and G: role of the 5'-sensor. J Biol Chem. 2009;284:31843-50 pubmed publisher
    ..Our results point to the importance of alternative RNA binding sites in RNase E and to alternative pathways of RNA recognition. ..
  28. McDowall K, Kaberdin V, Wu S, Cohen S, Lin Chao S. Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops. Nature. 1995;374:287-90 pubmed
    ..Modulation of RNase E cleavages by stem-loop regions and to a lesser extent by higher-order structure may explain why this enzyme, which does not have stringent sequence specificity, cleaves complex RNAs at a limited number of sites. ..
  29. Celesnik H, Deana A, Belasco J. Initiation of RNA decay in Escherichia coli by 5' pyrophosphate removal. Mol Cell. 2007;27:79-90 pubmed
    ..Moreover, these results reveal a striking parallel between the mechanisms of mRNA decay in prokaryotic and eukaryotic organisms. ..
  30. Ghora B, Apirion D. Structural analysis and in vitro processing to p5 rRNA of a 9S RNA molecule isolated from an rne mutant of E. coli. Cell. 1978;15:1055-66 pubmed
    A temperature-sensitive mutant of E. coli, rne (RNAase E-), accumulates a number of relatively small RNA molecules at the nonpermissive temperature. One of these molecules, 9S, contains 5S rRNA sequences...
  31. Redko Y, Tock M, Adams C, Kaberdin V, Grasby J, McDowall K. Determination of the catalytic parameters of the N-terminal half of Escherichia coli ribonuclease E and the identification of critical functional groups in RNA substrates. J Biol Chem. 2003;278:44001-8 pubmed
    ..Combined, these observations establish a foundation for the functional interpretation of a three-dimensional structure of the catalytic domain of RNaseE when solved. ..
  32. Babitzke P, Kushner S. The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli. Proc Natl Acad Sci U S A. 1991;88:1-5 pubmed
    The in vitro and in vivo analysis of the ribonuclease E-deficient (rne-) and the altered mRNA stability protein-deficient (ams-) strains of Escherichia coli has demonstrated that they carry mutations in the same structural gene...
  33. Ow M, Kushner S. Initiation of tRNA maturation by RNase E is essential for cell viability in E. coli. Genes Dev. 2002;16:1102-15 pubmed
    ..By using several new rne alleles, we have confirmed these observations and have also ruled out that M1 processing by RNase E is required for ..
  34. Diwa A, Belasco J. Critical features of a conserved RNA stem-loop important for feedback regulation of RNase E synthesis. J Biol Chem. 2002;277:20415-22 pubmed
    ..This endonuclease controls its own synthesis via a feedback mechanism in which the longevity of rne (RNase E) mRNA is modulated by a cis-acting sensory element that responds to changes in cellular RNase E activity...
  35. Urban J, Vogel J. Translational control and target recognition by Escherichia coli small RNAs in vivo. Nucleic Acids Res. 2007;35:1018-37 pubmed
    ..We expect our GFP fusion approach to be applicable to sRNA targets of other bacteria, and also demonstrate that Vibrio RyhB sRNA represses a Vibrio sodB fusion when co-expressed in E.coli. ..
  36. Vanderpool C. Physiological consequences of small RNA-mediated regulation of glucose-phosphate stress. Curr Opin Microbiol. 2007;10:146-51 pubmed
    ..The current model is that SgrS promotes recovery by stopping the synthesis of glucose transport proteins, which in turn limits the accumulation of toxic sugar-phosphates. ..
  37. Folichon M, Arluison V, Pellegrini O, Huntzinger E, Regnier P, Hajnsdorf E. The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation. Nucleic Acids Res. 2003;31:7302-10 pubmed
    ..In addition, RNase E processing, which occurred close to the U-rich sequence, is impaired by the presence of Hfq. These data suggest that Hfq modulates the sensitivity of RNA to ribonucleases in the cell. ..
  38. Khemici V, Poljak L, Luisi B, Carpousis A. The RNase E of Escherichia coli is a membrane-binding protein. Mol Microbiol. 2008;70:799-813 pubmed publisher
    ..This is the first report demonstrating that RNase E is a membrane-binding protein and that its localization to the inner cytoplasmic membrane is important for normal cell growth. ..
  39. Mackie G. Ribonuclease E is a 5'-end-dependent endonuclease. Nature. 1998;395:720-3 pubmed
    ..coli, the phenomenon of 'all or none' mRNA decay, and the stabilization provided by 5' stem-loop structures. ..
  40. Mohanty B, Kushner S. Analysis of the function of Escherichia coli poly(A) polymerase I in RNA metabolism. Mol Microbiol. 1999;34:1094-108 pubmed
    ..In contrast, the transcripts for rne (RNase E) and pnp (polynucleotide phosphorylase, PNPase), enzymes involved in mRNA decay, were stabilized...
  41. Kaberdin V. Probing the substrate specificity of Escherichia coli RNase E using a novel oligonucleotide-based assay. Nucleic Acids Res. 2003;31:4710-6 pubmed
    ..Moreover, the simplicity and efficiency of the proposed assay suggest that it can be a valuable tool not only for the characterization of RNase E homologs but also for the analysis of other site-specific nucleases. ..
  42. Leroy A, Vanzo N, Sousa S, Dreyfus M, Carpousis A. Function in Escherichia coli of the non-catalytic part of RNase E: role in the degradation of ribosome-free mRNA. Mol Microbiol. 2002;45:1231-43 pubmed
    ..The rne gene was replaced with alleles encoding deletions in the non-catalytic part of RNase E...
  43. Viegas S, Pfeiffer V, Sittka A, Silva I, Vogel J, Arraiano C. Characterization of the role of ribonucleases in Salmonella small RNA decay. Nucleic Acids Res. 2007;35:7651-64 pubmed
    ..Taken together, the results of this study provide initial insight into the mechanisms of sRNA decay in Salmonella, and indicate specific contributions of the RNA decay machinery components to the turnover of individual sRNAs. ..
  44. Li Z, Deutscher M. RNase E plays an essential role in the maturation of Escherichia coli tRNA precursors. RNA. 2002;8:97-109 pubmed
    ..coli tRNA transcripts, providing substrates for final maturation by RNase P and the 3' exoribonucleases. Based on this new information, a detailed model for tRNA maturation is proposed. ..
  45. Hajnsdorf E, Regnier P. E. coli RpsO mRNA decay: RNase E processing at the beginning of the coding sequence stimulates poly(A)-dependent degradation of the mRNA. J Mol Biol. 1999;286:1033-43 pubmed
    ..We conclude that 5' extremities modulate the poly(A)-dependent degradation of mRNA fragments and that the 5' cleavage by RNase E at M3 activates the chemical degradation of the rpsO mRNA. ..
  46. Lopez P, Marchand I, Joyce S, Dreyfus M. The C-terminal half of RNase E, which organizes the Escherichia coli degradosome, participates in mRNA degradation but not rRNA processing in vivo. Mol Microbiol. 1999;33:188-99 pubmed
    ..with other degradosome proteins; however, PNPase is not essential, as RNase E remains fully active towards mRNAs in rne+pnp mutants...
  47. Arraiano C, Yancey S, Kushner S. Stabilization of discrete mRNA breakdown products in ams pnp rnb multiple mutants of Escherichia coli K-12. J Bacteriol. 1988;170:4625-33 pubmed
    ..a series of multiple mutants containing the pnp-7 (polynucleotide phosphorylase), rnb-500 (RNase II), and ams-1 (altered message stability) alleles, it was possible to study general mRNA turnover as well as the degradation of ..
  48. McDowall K, Hernandez R, Lin Chao S, Cohen S. The ams-1 and rne-3071 temperature-sensitive mutations in the ams gene are in close proximity to each other and cause substitutions within a domain that resembles a product of the Escherichia coli mre locus. J Bacteriol. 1993;175:4245-9 pubmed
    Two temperature-sensitive mutations, ams-1 and rne-3071, in the ams (altered mRNA stability) gene have been used extensively to investigate the processing and decay of RNA in Escherichia coli...
  49. Claverie Martin F, Diaz Torres M, Yancey S, Kushner S. Cloning of the altered mRNA stability (ams) gene of Escherichia coli K-12. J Bacteriol. 1989;171:5479-86 pubmed
    A temperature-sensitive mutation in the ams gene of Escherichia coli causes an increase in the chemical half-life of pulse-labeled RNA at the nonpermissive temperature...
  50. Carpousis A, Van Houwe G, Ehretsmann C, Krisch H. Copurification of E. coli RNAase E and PNPase: evidence for a specific association between two enzymes important in RNA processing and degradation. Cell. 1994;76:889-900 pubmed
    ..coli 5S ribosomal RNA, bacteriophage T4 gene 32 mRNA and E. coli ompA mRNA at sites known to depend on the rne gene for cleavage in vivo...
  51. Callaghan A, Marcaida M, Stead J, McDowall K, Scott W, Luisi B. Structure of Escherichia coli RNase E catalytic domain and implications for RNA turnover. Nature. 2005;437:1187-91 pubmed
    ..The structure explains how the recognition of the 5' terminus of the substrate may trigger catalysis and also sheds light on the question of how RNase E might selectively process, rather than destroy, specific RNA precursors. ..
  52. Ehretsmann C, Carpousis A, Krisch H. Specificity of Escherichia coli endoribonuclease RNase E: in vivo and in vitro analysis of mutants in a bacteriophage T4 mRNA processing site. Genes Dev. 1992;6:149-59 pubmed
    ..Processing by RNase E was apparently inhibited by sequences that sequester the site in secondary structure. ..
  53. Casaregola S, Jacq A, Laoudj D, McGurk G, Margarson S, Tempete M, et al. Cloning and analysis of the entire Escherichia coli ams gene. ams is identical to hmp1 and encodes a 114 kDa protein that migrates as a 180 kDa protein. J Mol Biol. 1992;228:30-40 pubmed
    We have used an antibody to a previously identified 180 kDa (Hmp1) protein in Escherichia coli to clone the corresponding gene, which encodes a polypeptide of 114 kDa that has a mobility equivalent to 180 kDa in SDS/PAGE...
  54. Carpousis A. The RNA degradosome of Escherichia coli: an mRNA-degrading machine assembled on RNase E. Annu Rev Microbiol. 2007;61:71-87 pubmed
  55. Jiang X, Belasco J. Catalytic activation of multimeric RNase E and RNase G by 5'-monophosphorylated RNA. Proc Natl Acad Sci U S A. 2004;101:9211-6 pubmed
    ..Among the molecular mechanisms that could account for these properties are those in which 5'-end binding by one enzyme subunit induces a protein structural change that accelerates RNA cleavage by another subunit. ..
  56. Caruthers J, Feng Y, McKay D, Cohen S. Retention of core catalytic functions by a conserved minimal ribonuclease E peptide that lacks the domain required for tetramer formation. J Biol Chem. 2006;281:27046-51 pubmed publisher
    ..and cleavage site specificity characteristic of full-length RNase E and that also confer colony forming ability on rne null mutant bacteria. Further truncation leads to loss of these properties...
  57. Mohanty B, Kushner S. Polynucleotide phosphorylase, RNase II and RNase E play different roles in the in vivo modulation of polyadenylation in Escherichia coli. Mol Microbiol. 2000;36:982-94 pubmed
    ..Conversely, toxicity is significantly reduced in the presence of excess RNase II. Overproduction of RNase E leads to increased polyadenylation and no reduction in toxicity. ..
  58. Schuck A, Diwa A, Belasco J. RNase E autoregulates its synthesis in Escherichia coli by binding directly to a stem-loop in the rne 5' untranslated region. Mol Microbiol. 2009;72:470-8 pubmed publisher
    RNase E autoregulates its production in Escherichia coli by governing the decay rate of rne (RNase E) mRNA. It does so by a mechanism that is dependent in part on hp2, a cis-acting stem-loop within the rne 5' untranslated region...
  59. Mackie G. Specific endonucleolytic cleavage of the mRNA for ribosomal protein S20 of Escherichia coli requires the product of the ams gene in vivo and in vitro. J Bacteriol. 1991;173:2488-97 pubmed
    ..the activity of the putative endonuclease(s) depends strongly, both in vivo and in vitro, on the product of the ams gene, which is known to influence mRNA lifetimes in vivo...
  60. Taraseviciene L, Miczak A, Apirion D. The gene specifying RNase E (rne) and a gene affecting mRNA stability (ams) are the same gene. Mol Microbiol. 1991;5:851-5 pubmed
    ..this DNA fragment (110,000 Da), the restriction map of this fragment, the fact that the same clone complements the ams mutation, and the observation that the rne-3071 and the ams mutations cause similar patterns of RNA synthesis, show ..
  61. Mass E, Escorcia F, Gottesman S. Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli. Genes Dev. 2003;17:2374-83 pubmed publisher
    ..Thus, this large class of regulatory RNAs share an unexpected intrinsic mechanism for shutting off their action...
  62. Mudd E, Carpousis A, Krisch H. Escherichia coli RNase E has a role in the decay of bacteriophage T4 mRNA. Genes Dev. 1990;4:873-81 pubmed
    ..Although the effect of the rne temperature-sensitive mutation could be indirect, the simplest interpretation of our results is that RNase E acts ..
  63. Kushner S. mRNA decay in Escherichia coli comes of age. J Bacteriol. 2002;184:4658-65; discussion 4657 pubmed
  64. Mudd E, Higgins C. Escherichia coli endoribonuclease RNase E: autoregulation of expression and site-specific cleavage of mRNA. Mol Microbiol. 1993;9:557-68 pubmed
    Mutations in the Escherichia coli rne (ams) gene have a general effect on the rate of mRNA decay in vivo. Using antibodies we have shown that the product of the rne gene is a polypeptide of relative mobility 180 kDa...
  65. Hajnsdorf E, Braun F, Haugel Nielsen J, Regnier P. Polyadenylylation destabilizes the rpsO mRNA of Escherichia coli. Proc Natl Acad Sci U S A. 1995;92:3973-7 pubmed
    ..In contrast polyadenylylation does not significantly modify the stability of rpsO mRNA undergoing RNase E-mediated degradation. ..
  66. Taraseviciene L, Björk G, Uhlin B. Evidence for an RNA binding region in the Escherichia coli processing endoribonuclease RNase E. J Biol Chem. 1995;270:26391-8 pubmed
    The processing endoribonuclease RNase E (Rne), which is encoded by the rne gene, is involved in the maturation process of messenger RNAs and a ribosomal RNA...
  67. Dam Mikkelsen N, Gerdes K. Sok antisense RNA from plasmid R1 is functionally inactivated by RNase E and polyadenylated by poly(A) polymerase I. Mol Microbiol. 1997;26:311-20 pubmed
    ..We also show that Sok RNA is polyadenylated by poly(A) polymerase I (PAP I), and that the poly(A)-tailing is prerequisite for the rapid 3'-exonucleolytic degradation by PNPase. ..
  68. Söderbom F, Binnie U, Masters M, Wagner E. Regulation of plasmid R1 replication: PcnB and RNase E expedite the decay of the antisense RNA, CopA. Mol Microbiol. 1997;26:493-504 pubmed
    ..We also find that, as predicted, under conditions in which CopA synthesis is unaffected, pcnB mutation reduces RepA translation and increases CopA stability to the same extent. ..
  69. Kido M, Yamanaka K, Mitani T, Niki H, Ogura T, Hiraga S. RNase E polypeptides lacking a carboxyl-terminal half suppress a mukB mutation in Escherichia coli. J Bacteriol. 1996;178:3917-25 pubmed
    ..A linkage group (smbB) of the suppressor mutations is located in the rne/ams/hmp gene encoding the processing endoribonuclease RNase E...
  70. Mohanty B, Kushner S. Rho-independent transcription terminators inhibit RNase P processing of the secG leuU and metT tRNA polycistronic transcripts in Escherichia coli. Nucleic Acids Res. 2008;36:364-75 pubmed
    ..Our data also demonstrate that the Rne-1 protein retains significant activity on tRNA substrates at the non-permissive temperature...
  71. Marcaida M, DePristo M, Chandran V, Carpousis A, Luisi B. The RNA degradosome: life in the fast lane of adaptive molecular evolution. Trends Biochem Sci. 2006;31:359-65 pubmed
  72. O Hara E, Chekanova J, Ingle C, Kushner Z, Peters E, Kushner S. Polyadenylylation helps regulate mRNA decay in Escherichia coli. Proc Natl Acad Sci U S A. 1995;92:1807-11 pubmed
    ..The half-lives of individual mRNAs were increased in both a delta pcnB single mutant and a delta pcnB pnp-7 rnb-500 rne-1 multiple mutant...
  73. Regnier P, Hajnsdorf E. Decay of mRNA encoding ribosomal protein S15 of Escherichia coli is initiated by an RNase E-dependent endonucleolytic cleavage that removes the 3' stabilizing stem and loop structure. J Mol Biol. 1991;217:283-92 pubmed
    ..The other two cleavages are dependent on the wild-type allele of the rne gene, which encodes the endonucleolytic enzyme RNase E...
  74. Morita T, Mochizuki Y, Aiba H. Translational repression is sufficient for gene silencing by bacterial small noncoding RNAs in the absence of mRNA destruction. Proc Natl Acad Sci U S A. 2006;103:4858-63 pubmed
    ..One possible physiological role of mRNA degradation mediated by sRNP is to rid the cell of translationally inactive mRNAs, making gene silencing irreversible. ..
  75. Vanzo N, Li Y, Py B, Blum E, Higgins C, Raynal L, et al. Ribonuclease E organizes the protein interactions in the Escherichia coli RNA degradosome. Genes Dev. 1998;12:2770-81 pubmed
  76. Hajnsdorf E, Steier O, Coscoy L, Teysset L, Regnier P. Roles of RNase E, RNase II and PNPase in the degradation of the rpsO transcripts of Escherichia coli: stabilizing function of RNase II and evidence for efficient degradation in an ams pnp rnb mutant. EMBO J. 1994;13:3368-77 pubmed
    ..The enzyme(s) involved in this degradation pathway is not known. We detected an unstable elongated rpsO mRNA presumably resulting from the addition of nucleotides at the 3' end of the transcript. ..
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