recX

Summary

Gene Symbol: recX
Description: regulatory protein for RecA
Alias: ECK2693, JW2668, oraA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Venkatesh R, Ganesh N, Guhan N, Reddy M, Chandrasekhar T, Muniyappa K. RecX protein abrogates ATP hydrolysis and strand exchange promoted by RecA: insights into negative regulation of homologous recombination. Proc Natl Acad Sci U S A. 2002;99:12091-6 pubmed
    In many eubacteria, coexpression of recX with recA is essential for attenuation of the deleterious effects of recA overexpression; however, the molecular mechanism has remained enigmatic...
  2. Stohl E, Brockman J, Burkle K, Morimatsu K, Kowalczykowski S, Seifert H. Escherichia coli RecX inhibits RecA recombinase and coprotease activities in vitro and in vivo. J Biol Chem. 2003;278:2278-85 pubmed
    ..A gene designated recX (or oraA) is present directly downstream of recA in E. coli; however, the function of RecX is unknown...
  3. Lusetti S, Hobbs M, Stohl E, Chitteni Pattu S, Inman R, Seifert H, et al. The RecF protein antagonizes RecX function via direct interaction. Mol Cell. 2006;21:41-50 pubmed
    The RecX protein inhibits RecA filament extension, leading to net filament disassembly. The RecF protein physically interacts with the RecX protein and protects RecA from the inhibitory effects of RecX...
  4. Drees J, Lusetti S, Chitteni Pattu S, Inman R, Cox M. A RecA filament capping mechanism for RecX protein. Mol Cell. 2004;15:789-98 pubmed
    The RecX protein is a potent inhibitor of RecA protein activities. RecX functions by specifically blocking the extension of RecA filaments. In vitro, this leads to a net disassembly of RecA protein from circular single-stranded DNA...
  5. Drees J, Lusetti S, Cox M. Inhibition of RecA protein by the Escherichia coli RecX protein: modulation by the RecA C terminus and filament functional state. J Biol Chem. 2004;279:52991-7 pubmed
    The RecX protein is a potent inhibitor of RecA activities. We identified several factors that affect RecX-RecA interaction. The interaction is enhanced by the RecA C terminus and by significant concentrations of free Mg(2+) ion...
  6. Pages V, Koffel Schwartz N, Fuchs R. recX, a new SOS gene that is co-transcribed with the recA gene in Escherichia coli. DNA Repair (Amst). 2003;2:273-84 pubmed
    b>recX is a small open reading frame located downstream of recA that is conserved in many bacteria. In Escherichia coli, the recX gene (also named oraA) is a 501 bp open reading frame that encodes a predicted basic protein...
  7. Baitin D, Gruenig M, Cox M. SSB antagonizes RecX-RecA interaction. J Biol Chem. 2008;283:14198-204 pubmed publisher
    The RecX protein of Escherichia coli inhibits the extension of RecA protein filaments on DNA, presumably by binding to and blocking the growing filament end...
  8. Long J, Renzette N, Sandler S. Suppression of constitutive SOS expression by recA4162 (I298V) and recA4164 (L126V) requires UvrD and RecX in Escherichia coli K-12. Mol Microbiol. 2009;73:226-39 pubmed publisher
    ..RecA filament stability is negatively modulated by RecX and UvrD. recA730 (E38K) and recA4142 (F217Y) constitutively express the SOS response...
  9. Zaitsev E, Alexseyev A, Lanzov V, Satin L, Clark A. Nucleotide sequence between recA and alaSp in E. coli K12 and the sequence change in four recA mutations. Mutat Res. 1994;323:173-7 pubmed
    ..This sequence reveals an open reading frame of 166 codons we have named oraA. An NdeI restriction nuclease cleavage site also revealed by the sequence was used to clone, map and sequence three ..

More Information

Publications17

  1. Ragone S, Maman J, Furnham N, Pellegrini L. Structural basis for inhibition of homologous recombination by the RecX protein. EMBO J. 2008;27:2259-69 pubmed publisher
    ..Here, we describe a structural and functional analysis of the HR inhibitor protein RecX and its mode of interaction with the RecA filament...
  2. Lusetti S, Drees J, Stohl E, Seifert H, Cox M. The DinI and RecX proteins are competing modulators of RecA function. J Biol Chem. 2004;279:55073-9 pubmed
    The DinI and RecX proteins of Escherichia coli both modulate the function of RecA protein, but have very different effects. DinI protein stabilizes RecA filaments, preventing disassembly but permitting assembly...
  3. Mishra S, Mazumdar P, Dey J, Das A. Molecular modeling of RecX reveals its mode of interaction with RecA. Biochem Biophys Res Commun. 2003;312:615-22 pubmed
    The protein RecA is involved in homologous recombination, DNA repair and also catalyzes DNA strand exchange. RecX gene is downstream of recA and the gene product RecX is supposed to be important for RecA regulation...
  4. Stohl E, Blount L, Seifert H. Differential cross-complementation patterns of Escherichia coli and Neisseria gonorrhoeae RecA proteins. Microbiology. 2002;148:1821-31 pubmed
    ..Co-expression of the E. coli recA and recX genes in Gc suggests that in this heterologous system RecX modulates RecA-mediated processes.
  5. Van Dyk T, DeRose E, Gonye G. LuxArray, a high-density, genomewide transcription analysis of Escherichia coli using bioluminescent reporter strains. J Bacteriol. 2001;183:5496-505 pubmed publisher
    ..The responses of two of these, fusions to oraA and yigN, were induced in a LexA-dependent manner by both nalidixic acid and mitomycin C, identifying these as ..
  6. De Mot R, Schoofs G, Vanderleyden J. A putative regulatory gene downstream of recA is conserved in gram-negative and gram-positive bacteria. Nucleic Acids Res. 1994;22:1313-4 pubmed
  7. Chattoraj D. Tryptophanase in sRNA control of the Escherichia coli cell cycle. Mol Microbiol. 2007;63:1-3 pubmed
    ..Targeting a catabolic enzyme to buy time for recombination is an amazing example of adaptation, which illustrates the power of a selfish element (a plasmid in this case) to exploit the host cell machinery to its advantage. ..
  8. Renzette N, Gumlaw N, Sandler S. DinI and RecX modulate RecA-DNA structures in Escherichia coli K-12. Mol Microbiol. 2007;63:103-15 pubmed
    ..Biochemical observations show that at low ratios to RecA, DinI and RecX stabilize and destabilize RecA-DNA filaments, respectively, and that the C-terminal 17 residues of RecA are ..