recT

Summary

Gene Symbol: recT
Description: Rac prophage; recombination and repair protein
Alias: ECK1346, JW1343
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Kusano K, Takahashi N, Yoshikura H, Kobayashi I. Involvement of RecE exonuclease and RecT annealing protein in DNA double-strand break repair by homologous recombination. Gene. 1994;138:17-25 pubmed
    ..These include exonuclease VIII (Exo VIII), a 5' to 3' exonuclease working from the end of a duplex DNA, and RecT, an annealing protein. We found that a rac- strain (lacking the Rac prophage) cannot support this repair...
  2. Kolodner R, Hall S, Luisi DeLuca C. Homologous pairing proteins encoded by the Escherichia coli recE and recT genes. Mol Microbiol. 1994;11:23-30 pubmed
    ..and biochemical analysis of the recE gene region indicates there are two partially overlapping genes, recE and recT, encoding at least two proteins: exoVIII and the RecT protein...
  3. Muyrers J, Zhang Y, Buchholz F, Stewart A. RecE/RecT and Redalpha/Redbeta initiate double-stranded break repair by specifically interacting with their respective partners. Genes Dev. 2000;14:1971-82 pubmed
    ..break (DSB) repair by homologous recombination mediated by the 5'-3' exonuclease/annealing protein pairs, RecE/RecT and Redalpha/Redbeta, were analyzed...
  4. Shiraishi K, Imai Y, Yoshizaki S, Tadaki T, Ogata Y, Ikeda H. The role of UvrD in RecET-mediated illegitimate recombination in Escherichia coli. Genes Genet Syst. 2006;81:291-7 pubmed
    ..We have previously reported that coexpression of RecE and RecT enhances the frequency of recA-independent illegitimate recombination...
  5. Ikeda H, Shiraishi K, Ogata Y. Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli. Adv Biophys. 2004;38:3-20 pubmed
    ..In addition, we have shown that the overexpression of RecE and RecT enhances the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, RecO, ..
  6. Handa N, Kobayashi I. Accumulation of large non-circular forms of the chromosome in recombination-defective mutants of Escherichia coli. BMC Mol Biol. 2003;4:5 pubmed
    ..ii) In a recBC sbcA background, in which RecE-mediated recombination is active, recA, recJ, recQ, recE, recT, recF, recO, and recR mutations led to their accumulation...
  7. Noirot P, Gupta R, Radding C, Kolodner R. Hallmarks of homology recognition by RecA-like recombinases are exhibited by the unrelated Escherichia coli RecT protein. EMBO J. 2003;22:324-34 pubmed
    ..Here, we investigated whether the unrelated Escherichia coli RecT protein, which mediates homologous pairing and strand exchange, also exhibits such properties...
  8. Hall S, Kane M, Kolodner R. Identification and characterization of the Escherichia coli RecT protein, a protein encoded by the recE region that promotes renaturation of homologous single-stranded DNA. J Bacteriol. 1993;175:277-87 pubmed
    ..It appears that p33 is functionally analogous to the bacteriophage lambda beta protein, which may explain why RecE pathway recombination does not require recA. ..
  9. Hall S, Kolodner R. Homologous pairing and strand exchange promoted by the Escherichia coli RecT protein. Proc Natl Acad Sci U S A. 1994;91:3205-9 pubmed
    b>RecT protein of Escherichia coli promotes the formation of joint molecules between homologous linear double-stranded M13mp19 replicative-form bacteriophage DNA and circular single-stranded M13mp19 DNA in the presence of exonuclease VIII, ..

More Information

Publications20

  1. Clark A, Sharma V, Brenowitz S, Chu C, Sandler S, Satin L, et al. Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene. J Bacteriol. 1993;175:7673-82 pubmed
    ..of the Rac prophage of Escherichia coli K-12 reveals the presence of a partially overlapping reading frame we call recT. Deletion mutations show that recT is required for the RecE pathway of conjugational recombination...
  2. Barbour S, Nagaishi H, Templin A, Clark A. Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations. Proc Natl Acad Sci U S A. 1970;67:128-35 pubmed
    ..The ATP-independent DNase may be one of these enzymes. ..
  3. Takahashi N, Yoshikura H, Kobayashi I. An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks. Gene. 2003;303:89-97 pubmed
    ..This region was easily replaced by a RECT::Tn10 through general transduction and the resulting recT-negative derivative was defective in the conservative ..
  4. Brombacher E, Dorel C, Zehnder A, Landini P. The curli biosynthesis regulator CsgD co-ordinates the expression of both positive and negative determinants for biofilm formation in Escherichia coli. Microbiology. 2003;149:2847-57 pubmed
    ..coli by contemporary activation of adhesion positive determinants (the curli-encoding csg operons and the product of the yaiC gene) and repression of negative effectors such as yagS and pepD. ..
  5. Noirot P, Kolodner R. DNA strand invasion promoted by Escherichia coli RecT protein. J Biol Chem. 1998;273:12274-80 pubmed
    The RecT protein of Escherichia coli is a DNA-pairing protein required for the RecA-independent recombination events promoted by the RecE pathway...
  6. Shiraishi K, Hanada K, Iwakura Y, Ikeda H. Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli. J Bacteriol. 2002;184:4715-21 pubmed
    We analyzed effects of overexpression of RecE and RecT on illegitimate recombination during prophage induction in Escherichia coli and found that frequencies of spontaneous and UV-induced illegitimate recombination are enhanced by ..
  7. Clark A, Sandler S. Homologous genetic recombination: the pieces begin to fall into place. Crit Rev Microbiol. 1994;20:125-42 pubmed
    ..A second RecA-independent synapsis catalyzed by RecT (and RecE?) protein is now under study and a third type independent of both RecA and RecT has apparently been ..
  8. Ikeda H, Shiraishi K, Ogata Y. Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli. Adv Biophys. 2004;38:3-20 pubmed
    ..In addition, we have shown that the overexpression of RecE and RecT enhances the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, RecO, ..
  9. Kowalczykowski S, Dixon D, Eggleston A, Lauder S, Rehrauer W. Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev. 1994;58:401-65 pubmed
    ..this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as DNA polymerase I, DNA gyrase, DNA topoisomerase I, DNA ligase, ..
  10. Handa N, Kobayashi I. Type III restriction is alleviated by bacteriophage (RecE) homologous recombination function but enhanced by bacterial (RecBCD) function. J Bacteriol. 2005;187:7362-73 pubmed
    ..system, EcoP1 or EcoP15, is increased by the bacteriophage-mediated homologous recombination functions recE and recT of Rac prophage...
  11. Thresher R, Makhov A, Hall S, Kolodner R, Griffith J. Electron microscopic visualization of RecT protein and its complexes with DNA. J Mol Biol. 1995;254:364-71 pubmed
    Electron microscopy has been used to examine Escherichia coli RecT protein alone and in the complexes it forms with DNA substrates, with which it catalyzes strand exchange in vitro...