recQ

Summary

Gene Symbol: recQ
Description: ATP-dependent DNA helicase
Alias: ECK3816, JW5855
Species: Escherichia coli str. K-12 substr. MG1655
Products:     recQ

Top Publications

  1. Nakayama H, Nakayama K, Nakayama R, Irino N, Nakayama Y, Hanawalt P. Isolation and genetic characterization of a thymineless death-resistant mutant of Escherichia coli K12: identification of a new mutation (recQ1) that blocks the RecF recombination pathway. Mol Gen Genet. 1984;195:474-80 pubmed
    ..The original mutant was shown to carry an additional mutation probably in the vicinity of the uhp locus, which was also required for the full TLD resistance of the mutant to be expressed. ..
  2. Hanada K, Ukita T, Kohno Y, Saito K, Kato J, Ikeda H. RecQ DNA helicase is a suppressor of illegitimate recombination in Escherichia coli. Proc Natl Acad Sci U S A. 1997;94:3860-5 pubmed
    ..responsible for these diseases, BLM and WRN, have been cloned and identified as homologs of the Escherichia coli recQ genes...
  3. Harmon F, Kowalczykowski S. Biochemical characterization of the DNA helicase activity of the escherichia coli RecQ helicase. J Biol Chem. 2001;276:232-43 pubmed
    We demonstrate that RecQ helicase from Escherichia coli is a catalytic helicase whose activity depends on the concentration of ATP, free magnesium ion, and single-stranded DNA-binding (SSB) protein...
  4. Harmon F, Brockman J, Kowalczykowski S. RecQ helicase stimulates both DNA catenation and changes in DNA topology by topoisomerase III. J Biol Chem. 2003;278:42668-78 pubmed
    Together, RecQ helicase and topoisomerase III (Topo III) of Escherichia coli comprise a potent DNA strand passage activity that can catenate covalently closed DNA (Harmon, F. G., DiGate, R. J., and Kowalczykowski, S. C. (1999) Mol...
  5. Dou S, Wang P, Xu H, Xi X. The DNA binding properties of the Escherichia coli RecQ helicase. J Biol Chem. 2004;279:6354-63 pubmed
    The RecQ helicase family is highly conserved from bacteria to men and plays a conserved role in the preservation of genome integrity...
  6. Hishida T, Han Y, Shibata T, Kubota Y, Ishino Y, Iwasaki H, et al. Role of the Escherichia coli RecQ DNA helicase in SOS signaling and genome stabilization at stalled replication forks. Genes Dev. 2004;18:1886-97 pubmed
    The RecQ protein family is a highly conserved group of DNA helicases that play roles in maintaining genomic stability...
  7. Handa N, Ichige A, Kobayashi I. Contribution of RecFOR machinery of homologous recombination to cell survival after loss of a restriction-modification gene complex. Microbiology. 2009;155:2320-32 pubmed publisher
    ..Such resistance was also dependent on RecJ and RecQ functions. UV resistance of these non-null recBCD mutants is also reduced by recF, recJ or recQ mutation...
  8. Bernstein D, Keck J. Conferring substrate specificity to DNA helicases: role of the RecQ HRDC domain. Structure. 2005;13:1173-82 pubmed
    b>RecQ DNA helicases are multidomain enzymes that play pivotal roles in genome maintenance pathways...
  9. Garcia P, Bradley G, Hayes C, Krintel S, Soultanas P, Janscak P. RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases. Nucleic Acids Res. 2004;32:3771-8 pubmed
    Bloom (BLM) and Werner (WRN) syndrome proteins are members of the RecQ family of SF2 DNA helicases...

More Information

Publications67

  1. Bernstein D, Zittel M, Keck J. High-resolution structure of the E.coli RecQ helicase catalytic core. EMBO J. 2003;22:4910-21 pubmed
    b>RecQ family helicases catalyze critical genome maintenance reactions in bacterial and eukaryotic cells, playing key roles in several DNA metabolic processes. Mutations in recQ genes are linked to genome instability and human disease...
  2. Umezu K, Nakayama K, Nakayama H. Escherichia coli RecQ protein is a DNA helicase. Proc Natl Acad Sci U S A. 1990;87:5363-7 pubmed
    The Escherichia coli recQ gene, a member of the RecF recombination gene family, was set in an overexpression plasmid, and its product was purified to near-homogeneity...
  3. Xu H, Deprez E, Zhang A, Tauc P, Ladjimi M, Brochon J, et al. The Escherichia coli RecQ helicase functions as a monomer. J Biol Chem. 2003;278:34925-33 pubmed
    The RecQ helicases belong to an important family of highly conserved DNA helicases that play a key role in chromosomal maintenance, and their defects have been shown to lead to several disorders and cancer in humans...
  4. Courcelle J, Donaldson J, Chow K, Courcelle C. DNA damage-induced replication fork regression and processing in Escherichia coli. Science. 2003;299:1064-7 pubmed
    ..The reversed replication fork intermediate is stabilized by RecA and RecF and is degraded by the RecQ-RecJ helicase-nuclease when these proteins are absent...
  5. Magner D, Blankschien M, Lee J, Pennington J, Lupski J, Rosenberg S. RecQ promotes toxic recombination in cells lacking recombination intermediate-removal proteins. Mol Cell. 2007;26:273-86 pubmed
    The RecQ-helicase family is widespread, is highly conserved, and includes human orthologs that suppress genomic instability and cancer...
  6. Lestini R, Michel B. UvrD and UvrD252 counteract RecQ, RecJ, and RecFOR in a rep mutant of Escherichia coli. J Bacteriol. 2008;190:5995-6001 pubmed publisher
    ..We show here that the synthetic lethality of rep and uvrD mutations is also suppressed by recQ and recJ inactivation but not by rarA inactivation...
  7. Bernstein D, Keck J. Domain mapping of Escherichia coli RecQ defines the roles of conserved N- and C-terminal regions in the RecQ family. Nucleic Acids Res. 2003;31:2778-85 pubmed
    b>RecQ DNA helicases function in DNA replication, recombination and repair...
  8. Nakayama K, Irino N, Nakayama H. The recQ gene of Escherichia coli K12: molecular cloning and isolation of insertion mutants. Mol Gen Genet. 1985;200:266-71 pubmed
    The recQ gene of Escherichia coli K12 was subcloned from plasmid pKO1 (Oeda et al...
  9. Courcelle J, Hanawalt P. RecQ and RecJ process blocked replication forks prior to the resumption of replication in UV-irradiated Escherichia coli. Mol Gen Genet. 1999;262:543-51 pubmed
    ..Two other recF pathway proteins, the RecQ helicase and the RecJ exonuclease, have been shown to affect the sites and frequencies at which illegitimate ..
  10. Handa N, Morimatsu K, Lovett S, Kowalczykowski S. Reconstitution of initial steps of dsDNA break repair by the RecF pathway of E. coli. Genes Dev. 2009;23:1234-45 pubmed publisher
    ..seven-protein reaction that recapitulates early steps of dsDNA break repair using purified RecA, RecF, RecO, RecR, RecQ, RecJ, and SSB proteins, components of the RecF system...
  11. Harmon F, DiGate R, Kowalczykowski S. RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination. Mol Cell. 1999;3:611-20 pubmed
    E. coli RecQ protein is a multifunctional helicase with homologs that include the S. cerevisiae Sgs1 helicase and the H. sapiens Wrn and Blm helicases...
  12. Kocsis Z, Sarlós K, Harami G, Martina M, Kovacs M. A nucleotide-dependent and HRDC domain-dependent structural transition in DNA-bound RecQ helicase. J Biol Chem. 2014;289:5938-49 pubmed publisher
    The allosteric communication between the ATP- and DNA-binding sites of RecQ helicases enables efficient coupling of ATP hydrolysis to translocation along single-stranded DNA (ssDNA) and, in turn, the restructuring of multistranded DNA ..
  13. Harmon F, Kowalczykowski S. RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination. Genes Dev. 1998;12:1134-44 pubmed
    b>RecQ helicase is important to homologous recombination and DNA repair in Escherichia coli. We demonstrate that RecQ helicase, in conjunction with RecA and SSB proteins, can initiate recombination events in vitro...
  14. Lopez C, Yang S, Deibler R, Ray S, Pennington J, Digate R, et al. A role for topoisomerase III in a recombination pathway alternative to RuvABC. Mol Microbiol. 2005;58:80-101 pubmed
    ..Either recQ or recA null mutations, but not helD null or lexA3, partially rescued the synthetic lethality of the double ..
  15. Zhang B, Zhang A, Chen L, Xi X. Inhibition of DNA helicase, ATPase and DNA-binding activities of E. coli RecQ helicase by chemotherapeutic agents. J Biochem. 2008;143:773-9 pubmed publisher
    b>RecQ helicases play an essential role in maintaining genetic integrity in all organisms from Escherichia coli to humans...
  16. Ivanković S, Vujaklija D, Đermić D. Nucleolytic degradation of 3'-ending overhangs is essential for DNA-end resection in RecA-loading deficient recB mutants of Escherichia coli. DNA Repair (Amst). 2017;57:56-65 pubmed publisher
    ..illegitimate recombination in the recB1080 mutant but not in a wt strain, while working in concert with the RecQ helicase...
  17. Mashimo K, Kawata M, Yamamoto K. Roles of the RecJ and RecQ proteins in spontaneous formation of deletion mutations in the Escherichia coli K12 endogenous tonB gene. Mutagenesis. 2003;18:355-63 pubmed
    ..of Escherichia coli was used as a target for spontaneous deletion mutations which were isolated from recJ(-) and recQ(-) cells. Large deletions, due to simultaneous mutations of the trp operon, were also isolated...
  18. Hanada K, Hickson I. Molecular genetics of RecQ helicase disorders. Cell Mol Life Sci. 2007;64:2306-22 pubmed
    The RecQ helicases belong to the Superfamily II group of DNA helicases, and are defined by amino acid motifs that show sequence similarity to the catalytic domain of Escherichia coli RecQ...
  19. Li N, Henry E, Guiot E, Rigolet P, Brochon J, Xi X, et al. Multiple Escherichia coli RecQ helicase monomers cooperate to unwind long DNA substrates: a fluorescence cross-correlation spectroscopy study. J Biol Chem. 2010;285:6922-36 pubmed publisher
    The RecQ family helicases catalyze the DNA unwinding reaction in an ATP hydrolysis-dependent manner...
  20. Usongo V, Drolet M. Roles of type 1A topoisomerases in genome maintenance in Escherichia coli. PLoS Genet. 2014;10:e1004543 pubmed publisher
    In eukaryotes, type 1A topoisomerases (topos) act with RecQ-like helicases to maintain the stability of the genome. Despite having been the first type 1A enzymes to be discovered, much less is known about the involvement of the E...
  21. Harami G, Nagy N, Martina M, Neuman K, Kovács M. The HRDC domain of E. coli RecQ helicase controls single-stranded DNA translocation and double-stranded DNA unwinding rates without affecting mechanoenzymatic coupling. Sci Rep. 2015;5:11091 pubmed publisher
    DNA-restructuring activities of RecQ-family helicases play key roles in genome maintenance...
  22. Zhang X, Dou S, Xie P, Wang P, Xi X. RecQ helicase-catalyzed DNA unwinding detected by fluorescence resonance energy transfer. Acta Biochim Biophys Sin (Shanghai). 2005;37:593-600 pubmed
    A fluorometric assay was used to study the DNA unwinding kinetics induced by Escherichia coli RecQ helicase...
  23. Courcelle J, Hanawalt P. Participation of recombination proteins in rescue of arrested replication forks in UV-irradiated Escherichia coli need not involve recombination. Proc Natl Acad Sci U S A. 2001;98:8196-202 pubmed
    ..The products of the recJ and recQ genes process the blocked replication forks before the resumption of replication and may affect the fidelity of the ..
  24. Heyer W. Damage signaling: RecQ sends an SOS to you. Curr Biol. 2004;14:R895-7 pubmed
    The DNA helicase RecQ is required for proper induction of the SOS response to replication stress in Escherichia coli...
  25. Buljubasic M, Zahradka D, Zahradka K. RecQ helicase acts before RuvABC, RecG and XerC proteins during recombination in recBCD sbcBC mutants of Escherichia coli. Res Microbiol. 2013;164:987-97 pubmed publisher
    The RecQ helicase is required by the RecF recombination pathway that is operative in recBC(D) sbcB sbcC(D) mutants of Escherichia coli...
  26. Sukhodolets V. [Formation of the heterozygous tandem duplication in the process of conjugation recombination in Escherichia coli: study of the effect of mutations for the recQ, uvrD, and recJ genes]. Genetika. 1999;35:450-8 pubmed
    ..The effect of mutations for the recQ, uvrD, and recJ genes on the frequency of duplications was studied...
  27. Seki M, Tada S, Enomoto T. Function of recQ family helicase in genome stability. Subcell Biochem. 2006;40:49-73 pubmed
    The recQ gene of Escherichia coli is the founding member of the RecQ family of helicases. Like E. coli, lower eukaryotic species also possess single RecQ proteins, such as Sgs1 and Rqh1 in budding and fission yeast, respectively...
  28. Nakayama H. Escherichia coli RecQ helicase: a player in thymineless death. Mutat Res. 2005;577:228-36 pubmed
    DNA helicases of the RecQ family are distributed among most organisms and are thought to play important roles in various aspects of DNA metabolism...
  29. Ivancic Bace I, Salaj Smic E, Brcic Kostic K. Effects of recJ, recQ, and recFOR mutations on recombination in nuclease-deficient recB recD double mutants of Escherichia coli. J Bacteriol. 2005;187:1350-6 pubmed
    ..RecBCD enzyme performs all of these activities, whereas the recombination machinery of the RecF pathway consists of RecQ (helicase), RecJ (5'-->3' exonuclease), and RecFOR (RecA-single-stranded DNA filament formation)...
  30. Salem A, Nakano T, Takuwa M, Matoba N, Tsuboi T, Terato H, et al. Genetic analysis of repair and damage tolerance mechanisms for DNA-protein cross-links in Escherichia coli. J Bacteriol. 2009;191:5657-68 pubmed publisher
    ..for HR, RR, and NER, the mutation of genes involved in several aspects of DNA repair and transactions, such as recQ, xth nfo, dksA, and topA, rendered cells slightly but significantly sensitive to FA but not azaC, possibly ..
  31. Courcelle C, Chow K, Casey A, Courcelle J. Nascent DNA processing by RecJ favors lesion repair over translesion synthesis at arrested replication forks in Escherichia coli. Proc Natl Acad Sci U S A. 2006;103:9154-9 pubmed
    ..Here, we show that the RecJ nuclease and RecQ helicase, which partially degrade the nascent DNA at blocked replication forks, are required for the rapid recovery ..
  32. Sukhodolets V, Botina S, Trenina M, Ukhabotina L. [Homologous recombination and chromosomal rearrangements in Escherichia coli strains carrying a heterozygous tandem duplication]. Genetika. 2001;37:591-601 pubmed
    ..DNA repeats in the duplication was studied in strains carrying different combinations of recBC, sbcBC, recB::Tn10, recQ::Tn3 mutations...
  33. Chow K, Courcelle J. RecBCD and RecJ/RecQ initiate DNA degradation on distinct substrates in UV-irradiated Escherichia coli. Radiat Res. 2007;168:499-506 pubmed
    ..Instead, we observed that the nascent DNA at arrested replication forks in recA cultures is degraded by RecJ/RecQ, similar to what occurs in wild-type cultures...
  34. Shereda R, Reiter N, Butcher S, Keck J. Identification of the SSB binding site on E. coli RecQ reveals a conserved surface for binding SSB's C terminus. J Mol Biol. 2009;386:612-25 pubmed publisher
    b>RecQ DNA helicases act in conjunction with heterologous partner proteins to catalyze DNA metabolic activities, including recombination initiation and stalled replication fork processing...
  35. Umezu K, Nakayama H. RecQ DNA helicase of Escherichia coli. Characterization of the helix-unwinding activity with emphasis on the effect of single-stranded DNA-binding protein. J Mol Biol. 1993;230:1145-50 pubmed
    b>RecQ protein of Escherichia coli is a DNA helicase implicated in the RecF pathway of genetic recombination...
  36. Morimatsu K, Kowalczykowski S. RecQ helicase and RecJ nuclease provide complementary functions to resect DNA for homologous recombination. Proc Natl Acad Sci U S A. 2014;111:E5133-42 pubmed publisher
    ..DNA repair by the RecF pathway of Escherichia coli requires the coordinated activities of RecA, RecFOR, RecQ, RecJ, and single-strand DNA binding (SSB) proteins...
  37. Zittel M, Keck J. Coupling DNA-binding and ATP hydrolysis in Escherichia coli RecQ: role of a highly conserved aromatic-rich sequence. Nucleic Acids Res. 2005;33:6982-91 pubmed
    b>RecQ enzymes are broadly conserved Superfamily-2 (SF-2) DNA helicases that play critical roles in DNA metabolism...
  38. Seol Y, Strub M, Neuman K. Single molecule measurements of DNA helicase activity with magnetic tweezers and t-test based step-finding analysis. Methods. 2016;105:119-27 pubmed publisher
    ..single-molecule unwinding assay and the analysis routine, we provide DNA unwinding measurements of Escherichia coli RecQ helicase under a variety of conditions (Na+, ATP, temperature, and DNA substrate geometry)...
  39. Lohman T. Escherichia coli DNA helicases: mechanisms of DNA unwinding. Mol Microbiol. 1992;6:5-14 pubmed
    ..coli Rep, RecBCD and phage T7 gene 4 helicases. ..
  40. Zhang X, Dou S, Xie P, Hu J, Wang P, Xi X. Escherichia coli RecQ is a rapid, efficient, and monomeric helicase. J Biol Chem. 2006;281:12655-63 pubmed
    b>RecQ family helicases play a key role in chromosome maintenance. Despite extensive biochemical, biophysical, and structural studies, the mechanism by which helicase unwinds double-stranded DNA remains to be elucidated...
  41. Yang H, Wolff E, Kim M, Diep A, Miller J. Identification of mutator genes and mutational pathways in Escherichia coli using a multicopy cloning approach. Mol Microbiol. 2004;53:283-95 pubmed
    ..Other identified genes include those encoding DNA helicases (UvrD, RecG, RecQ), truncated forms of the DNA mismatch repair protein (MutS) and a primosomal component (DnaT), a negative modulator ..
  42. Irino N, Nakayama K, Nakayama H. The recQ gene of Escherichia coli K12: primary structure and evidence for SOS regulation. Mol Gen Genet. 1986;205:298-304 pubmed
    A 2,695 bp chromosomal segment of Escherichia coli K12 containing the recQ gene was sequenced...
  43. Bachrati C, Hickson I. Analysis of the DNA unwinding activity of RecQ family helicases. Methods Enzymol. 2006;409:86-100 pubmed
    The RecQ family of DNA helicases is highly conserved in evolution from bacteria to mammals. There are five human RecQ family members (RECQ1, BLM, WRN, RECQ4 and RECQ5), defects, three of which give rise to inherited human disorders...
  44. Isaksson L, Takata R. The temperature sensitive mutant 72c. I. Pleiotropic growth behaviour and changed response to some antibiotics and mutations in the transcription or translation apparatus. Mol Gen Genet. 1978;161:9-14 pubmed
    ..coli chromosome. The gene order is lip-fusB-supE. ..
  45. Handa N, Kobayashi I. Accumulation of large non-circular forms of the chromosome in recombination-defective mutants of Escherichia coli. BMC Mol Biol. 2003;4:5 pubmed
    ..ii) In a recBC sbcA background, in which RecE-mediated recombination is active, recA, recJ, recQ, recE, recT, recF, recO, and recR mutations led to their accumulation...
  46. Shiraishi K, Hanada K, Iwakura Y, Ikeda H. Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli. J Bacteriol. 2002;184:4715-21 pubmed
    ..On the other hand, the frequency of the RecET-mediated illegitimate recombination was enhanced by a recQ mutation, implying that the RecQ protein plays a role in suppression of RecET-mediated illegitimate recombination...
  47. Ikeda H, Shiraishi K, Ogata Y. Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli. Adv Biophys. 2004;38:3-20 pubmed
    ..has been shown that the RecJ exonuclease promotes short homology-dependent illegitimate recombination, but that the RecQ helicase suppresses it...
  48. Sanchez H, Kidane D, Castillo Cozar M, Graumann P, Alonso J. Recruitment of Bacillus subtilis RecN to DNA double-strand breaks in the absence of DNA end processing. J Bacteriol. 2006;188:353-60 pubmed
    ..Mutations in addA and addB or null mutations in recJ (DeltarecJ), recQ (DeltarecQ), or recS (DeltarecS) genes, when present in otherwise-Rec+ cells, render cells moderately sensitive to ..
  49. Kowalczykowski S, Dixon D, Eggleston A, Lauder S, Rehrauer W. Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev. 1994;58:401-65 pubmed
    ..At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as DNA polymerase I, DNA gyrase, DNA topoisomerase I, DNA ..
  50. Aldea M, Maples V, Kushner S. Generation of a detailed physical and genetic map of the ilv-metE-udp region of the Escherichia coli chromosome. J Mol Biol. 1988;200:427-38 pubmed
    ..structural genes have been identified, the cya and metE genes have been physically located, and the direction of recQ gene transcription has been determined...
  51. Ivancic Bace I, Vlasic I, Salaj Smic E, Brcic Kostic K. Genetic evidence for the requirement of RecA loading activity in SOS induction after UV irradiation in Escherichia coli. J Bacteriol. 2006;188:5024-32 pubmed
    ..We found that RecA loading activity is essential for SOS induction. In the recB1080 mutant RecQ helicase is not important, whereas RecJ nuclease slightly decreases SOS induction after UV irradiation...
  52. Shereda R, Bernstein D, Keck J. A central role for SSB in Escherichia coli RecQ DNA helicase function. J Biol Chem. 2007;282:19247-58 pubmed
    b>RecQ DNA helicases are critical components of DNA replication, recombination, and repair machinery in all eukaryotes and bacteria...
  53. Liu J, Rigolet P, Dou S, Wang P, Xi X. The zinc finger motif of Escherichia coli RecQ is implicated in both DNA binding and protein folding. J Biol Chem. 2004;279:42794-802 pubmed
    The RecQ family of DNA helicases has been shown to be important for the maintenance of genomic integrity. Mutations in human RecQ genes lead to genomic instability and cancer...
  54. Hanada K, Iwasaki M, Ihashi S, Ikeda H. UvrA and UvrB suppress illegitimate recombination: synergistic action with RecQ helicase. Proc Natl Acad Sci U S A. 2000;97:5989-94 pubmed
    ..Moreover, illegitimate recombination was synergistically enhanced by the recQ uvrA double mutation...
  55. Ikeda H, Shiraishi K, Ogata Y. Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli. Adv Biophys. 2004;38:3-20 pubmed
    ..has been shown that the RecJ exonuclease promotes short homology-dependent illegitimate recombination, but that the RecQ helicase suppresses it...
  56. Mendonca V, Klepin H, Matson S. DNA helicases in recombination and repair: construction of a delta uvrD delta helD delta recQ mutant deficient in recombination and repair. J Bacteriol. 1995;177:1326-35 pubmed
    ..Escherichia coli helicases II (uvrD), IV (helD), and RecQ (recQ) have all been implicated in the presynaptic stage of recombination in the RecF pathway...
  57. Sarlós K, Gyimesi M, Kele Z, Kovács M. Mechanism of RecQ helicase mechanoenzymatic coupling reveals that the DNA interactions of the ADP-bound enzyme control translocation run terminations. Nucleic Acids Res. 2015;43:1090-7 pubmed publisher
    The processing of various DNA structures by RecQ helicases is crucial for genome maintenance in both bacteria and eukaryotes...
  58. Mills M, Harami G, Seol Y, Gyimesi M, Martina M, Kovács Z, et al. RecQ helicase triggers a binding mode change in the SSB-DNA complex to efficiently initiate DNA unwinding. Nucleic Acids Res. 2017;45:11878-11890 pubmed publisher
    ..One example is the genome maintenance protein RecQ helicase...