recN

Summary

Gene Symbol: recN
Description: recombination and repair protein
Alias: ECK2612, JW5416, radB
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Rotering H, Braun V. Lipid deficiency in a lipoprotein mutant of Escherichia coli. FEBS Lett. 1977;83:41-4 pubmed
  2. Dunman P, Ren L, Rahman M, Palejwala V, Murphy H, Volkert M, et al. Escherichia coli cells defective for the recN gene display constitutive elevation of mutagenesis at 3,N(4)-ethenocytosine via an SOS-induced mechanism. Mol Microbiol. 2000;37:680-6 pubmed
    ..mutant bank of cells defective for 1-methyl-3-nitro-1-nitrosoguanidine-inducible genes revealed that defects in the recN gene cause a constitutive elevation of mutagenesis at epsilon C residues...
  3. Keyamura K, Sakaguchi C, Kubota Y, Niki H, Hishida T. RecA protein recruits structural maintenance of chromosomes (SMC)-like RecN protein to DNA double-strand breaks. J Biol Chem. 2013;288:29229-37 pubmed publisher
    Escherichia coli RecN is an SMC (structural maintenance of chromosomes) family protein that is required for DNA double-strand break (DSB) repair...
  4. Finch P, Chambers P, Emmerson P. Identification of the Escherichia coli recN gene product as a major SOS protein. J Bacteriol. 1985;164:653-8 pubmed
    ..The 62-kDa protein, which was induced to high intracellular levels, is the product of recN, a gene required for recBC-independent recombination...
  5. Almeida E, Fuentes J, Cuetara E, Prieto E, Llagostera M. Amifostine protection against induced DNA damage in gamma-irradiated Escherichia coli cells depend on recN DNA repair gene product activity. Environ Toxicol. 2010;25:130-6 pubmed publisher
    ..coli wild-type genotypes and in uvr, recF, recB, recB-recC-recF mutant strains, but not in recN defective cells...
  6. Sargentini N, Smith K. Genetic and phenotypic analyses indicating occurrence of the recN262 and radB101 mutations at the same locus in Escherichia coli. J Bacteriol. 1988;170:2392-4 pubmed
    ..Complementation tests involving attempts to reconstitute a radB+ recN+ strain by transductions between radB101 and recN262 donors and recipients, and tests involving plasmids ..
  7. Lloyd R, Picksley S, Prescott C. Inducible expression of a gene specific to the RecF pathway for recombination in Escherichia coli K12. Mol Gen Genet. 1983;190:162-7 pubmed
    ..These observations demonstrate the existence of an inducible gene which is regulated by lexA and whose expression is required for RecF recombination and DNA repair. ..
  8. Sargentini N, Smith K. Characterization of an Escherichia coli mutant (radB101) sensitive to gamma and uv radiation, and methyl methanesulfonate. Radiat Res. 1983;93:461-78 pubmed
    ..The radB mutant was normal for gamma- and uv-radiation mutagenesis, it showed only a slight enhancement of gamma- and uv-..
  9. Kowalczykowski S, Dixon D, Eggleston A, Lauder S, Rehrauer W. Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev. 1994;58:401-65 pubmed
    ..At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as DNA polymerase I, DNA gyrase, DNA ..

More Information

Publications25

  1. Odsbu I, Skarstad K. DNA compaction in the early part of the SOS response is dependent on RecN and RecA. Microbiology. 2014;160:872-82 pubmed publisher
    ..proteins RecA, RecO and RecR as well as the SOS-inducible, SMC (structural maintenance of chromosomes)-like protein RecN. RecN protein is produced in high amounts during the first part of the SOS response...
  2. Lestini R, Michel B. UvrD controls the access of recombination proteins to blocked replication forks. EMBO J. 2007;26:3804-14 pubmed
    ..UvrD action on RecA is conserved in evolution as it can be performed in E. coli by the UvrD homologue from Bacillus subtilis, PcrA. ..
  3. Wade J, Struhl K. Association of RNA polymerase with transcribed regions in Escherichia coli. Proc Natl Acad Sci U S A. 2004;101:17777-82 pubmed
    ..These observations suggest that in vivo association of sigma(70) and NusA with elongating RNAP is regulated by growth conditions. ..
  4. Kosa J, Zdraveski Z, Currier S, Marinus M, Essigmann J. RecN and RecG are required for Escherichia coli survival of Bleomycin-induced damage. Mutat Res. 2004;554:149-57 pubmed
    ..Strains recBCD and recN, which lack proteins required for double strand break (DSB) repair, were highly sensitive to BLM, while recF cells ..
  5. Picksley S, Attfield P, Lloyd R. Repair of DNA double-strand breaks in Escherichia coli K12 requires a functional recN product. Mol Gen Genet. 1984;195:267-74 pubmed
    Mutation of the recN gene of Escherichia coli in a recBC sbcB genetic background blocks conjugational recombination and confers increased sensitivity to UV light and mitomycin C...
  6. Sargentini N, Smith K. Quantitation of the involvement of the recA, recB, recC, recF, recJ, recN, lexA, radA, radB, uvrD, and umuC genes in the repair of X-ray-induced DNA double-strand breaks in Escherichia coli. Radiat Res. 1986;107:58-72 pubmed
    ..of X-ray-induced DSB: (I) the recA, recB, recC, and lexA mutants were completely deficient; (II) the radB and recN mutants were about 90% deficient; (III) the recF and recJ mutants were about 70% deficient; (IV) the radA and uvrD ..
  7. Wang T, Smith K. Different effects of recJ and recN mutations on the postreplication repair of UV-damaged DNA in Escherichia coli K-12. J Bacteriol. 1988;170:2555-9 pubmed
    ..did not affect the UV radiation sensitivity of uvrB and uvrB recF cells, but it increased the sensitivity of uvrB recN (approximately 3-fold) and uvrB recB (approximately 8-fold) cells...
  8. Meddows T, Savory A, Grove J, Moore T, Lloyd R. RecN protein and transcription factor DksA combine to promote faithful recombinational repair of DNA double-strand breaks. Mol Microbiol. 2005;57:97-110 pubmed
    ..When breaks are induced at a single site, the SOS-inducible RecN protein and the transcription factor DksA combine to promote efficient repair...
  9. Picksley S, Morton S, Lloyd R. The recN locus of Escherichia coli K12: molecular analysis and identification of the gene product. Mol Gen Genet. 1985;201:301-7 pubmed
    The recN gene which is necessary for inducible DNA repair and recombination in Escherichia coli has been cloned into the low copy plasmid vector pHSG415. Analysis of the recombinant plasmid, pSP100, revealed a 5...
  10. Fujii S, Fuchs R. Biochemical basis for the essential genetic requirements of RecA and the beta-clamp in Pol V activation. Proc Natl Acad Sci U S A. 2009;106:14825-30 pubmed publisher
    ..The present article presents an integrated view of TLS under physiologically relevant conditions in E. coli that may represent a paradigm for lesion bypass in other organisms. ..
  11. Pruteanu M, Baker T. Proteolysis in the SOS response and metal homeostasis in Escherichia coli. Res Microbiol. 2009;160:677-83 pubmed publisher
  12. Grove J, Wood S, Briggs G, Oldham N, Lloyd R. A soluble RecN homologue provides means for biochemical and genetic analysis of DNA double-strand break repair in Escherichia coli. DNA Repair (Amst). 2009;8:1434-43 pubmed publisher
    b>RecN is a highly conserved, SMC-like protein in bacteria. It plays an important role in the repair of DNA double-strand breaks and is therefore a key factor in maintaining genome integrity...
  13. Cupido M, Bridges B. Uvr-independent repair of 8-methoxypsoralen crosslinks in Escherichia coli: evidence for a recombinational process. Mutat Res. 1985;146:135-41 pubmed
    ..coli strains lacking a functional uvrABC endonuclease, is shown to require the products of the recA, recB, recF and recN genes...
  14. Nagashima K, Kubota Y, Shibata T, Sakaguchi C, Shinagawa H, Hishida T. Degradation of Escherichia coli RecN aggregates by ClpXP protease and its implications for DNA damage tolerance. J Biol Chem. 2006;281:30941-6 pubmed
    ..Escherichia coli recN is a member of the structural maintenance of chromosomes family and is required for DNA double strand break (DSB) ..
  15. Rostas K, Morton S, Picksley S, Lloyd R. Nucleotide sequence and LexA regulation of the Escherichia coli recN gene. Nucleic Acids Res. 1987;15:5041-9 pubmed
    The nucleotide sequence of a 2224 bp region of the Escherichia coli chromosome that carries the LexA regulated recN gene has been determined...
  16. Miczak A, Chauhan A, Apirion D. Two new genes located between 2758 and 2761 kilobase pairs on the Escherichia coli genome. J Bacteriol. 1991;173:3271-2 pubmed