recE

Summary

Gene Symbol: recE
Description: Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease
Alias: ECK1347, JW1344, rmuB, sbcA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Clark A. rec genes and homologous recombination proteins in Escherichia coli. Biochimie. 1991;73:523-32 pubmed
    ..The genes are usually grouped into three pathways named RecBCD, RecE and RecF for prominent genes which function in each...
  2. Ikeda H, Shiraishi K, Ogata Y. Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli. Adv Biophys. 2004;38:3-20 pubmed
    ..In addition, we have shown that the overexpression of RecE and RecT enhances the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, ..
  3. Ishioka K, Iwasaki H, Shinagawa H. Roles of the recG gene product of Escherichia coli in recombination repair: effects of the delta recG mutation on cell division and chromosome partition. Genes Genet Syst. 1997;72:91-9 pubmed
    ..We suggest that the defect in the processing in the recG mutant results in accumulation of nonpartitioned chromosomes, which are linked by Holliday junctions. ..
  4. Gillen J, Willis D, Clark A. Genetic analysis of the RecE pathway of genetic recombination in Escherichia coli K-12. J Bacteriol. 1981;145:521-32 pubmed
    The RecE pathway of genetic recombination in Escherichia coli K-12 was defined to be the pathway that is utilized in deoxyribonucleic acid exonuclease V (ExoV)-defective cells which express constitutively recE+, the structural gene for ..
  5. Mahajan S, Chu C, Willis D, Templin A, Clark A. Physical analysis of spontaneous and mutagen-induced mutants of Escherichia coli K-12 expressing DNA exonuclease VIII activity. Genetics. 1990;125:261-73 pubmed
    ..The nucleotide sequence shows that a translational gene fusion has occurred. The N-terminal 294 codons of recE have been deleted and the remaining C-terminal codons have been fused to the N-terminal portion of another reading ..
  6. Kaiser K, Murray N. Physical characterisation of the "Rac prophage" in E. coli K12. Mol Gen Genet. 1979;175:159-74 pubmed
    ..derivatives of phage lambda in which the phage recombination functions have been replaced by analogous functions (RecE) derived from the host chromosome (Gottesman et al., 1974; Gillen et al., 1977)...
  7. Fouts K, Wasie Gilbert T, Willis D, Clark A, Barbour S. Genetic analysis of transposon-induced mutations of the Rac prophage in Escherichia coli K-12 which affect expression and function of recE. J Bacteriol. 1983;156:718-26 pubmed
    ..Because of their phenotypic similarity to sbcA and sbcB mutations, which also suppress recB21 recC22, we have called them sbc mutations...
  8. Clark A, Satin L, Chu C. Transcription of the Escherichia coli recE gene from a promoter in Tn5 and IS50. J Bacteriol. 1994;176:7024-31 pubmed
    Six sbc::Tn5 insertions and one sbc::IS50 insertion, which cause recE expression in Escherichia coli, have been cloned, and their DNA sequences have been determined...
  9. Luisi DeLuca C, Clark A, Kolodner R. Analysis of the recE locus of Escherichia coli K-12 by use of polyclonal antibodies to exonuclease VIII. J Bacteriol. 1988;170:5797-805 pubmed
    ..This analysis showed that two point sbcA mutations (sbcA5 and sbcA23) and the sbc insertion mutations led to the synthesis of the 140-kilodalton (kDa) ..

More Information

Publications28

  1. Willis D, Satin L, Clark A. Mutation-dependent suppression of recB21 recC22 by a region cloned from the Rac prophage of Escherichia coli K-12. J Bacteriol. 1985;162:1166-72 pubmed
    ..By substituting the corresponding 2.45-kilobase EcoRI-toHindIII fragments of Rac prophage isolated from sbcA+, sbcA6, and sbcA23 strains for the shortened segment of one of the deletion mutant plasmids, we were able to show ..
  2. Shiraishi K, Imai Y, Yoshizaki S, Tadaki T, Ogata Y, Ikeda H. The role of UvrD in RecET-mediated illegitimate recombination in Escherichia coli. Genes Genet Syst. 2006;81:291-7 pubmed
    ..We have previously reported that coexpression of RecE and RecT enhances the frequency of recA-independent illegitimate recombination...
  3. Handa N, Kobayashi I. Accumulation of large non-circular forms of the chromosome in recombination-defective mutants of Escherichia coli. BMC Mol Biol. 2003;4:5 pubmed
    ..ii) In a recBC sbcA background, in which RecE-mediated recombination is active, recA, recJ, recQ, recE, recT, recF, recO, and recR mutations led to their ..
  4. Bitner R, Kuempel P. P1 transduction map spanning the replication terminus of Escherichia coli K12. Mol Gen Genet. 1981;184:208-12 pubmed
    ..1), zdc-235::Tn10 (min 32.3), zdd-230::Tn9 (min 33.3), and zde-234::Tn10 (min 34.2). The size of this region as determined by P1 cotransduction is very similar to previous estimates obtained by bacterial conjugation. ..
  5. Chu C, Templin A, Clark A. Suppression of a frameshift mutation in the recE gene of Escherichia coli K-12 occurs by gene fusion. J Bacteriol. 1989;171:2101-9 pubmed
    The nucleotide sequences of a small gene, racC, and the adjacent N-terminal half of the wild-type recE gene are presented...
  6. Evans R, Seeley N, Kuempel P. Loss of rac locus DNA in merozygotes of Escherichia coli K12. Mol Gen Genet. 1979;175:245-50 pubmed
    DNA-DNA hybridization was used to demonstrate that the substituted DNA in the bacteriophage lambda recE (formerly called lambda reverse) is homologous to DNA at the rac locus in Escherichia coli...
  7. Clark A, Sandler S. Homologous genetic recombination: the pieces begin to fall into place. Crit Rev Microbiol. 1994;20:125-42 pubmed
    ..A second RecA-independent synapsis catalyzed by RecT (and RecE?) protein is now under study and a third type independent of both RecA and RecT has apparently been discovered...
  8. Clark A, Sharma V, Brenowitz S, Chu C, Sandler S, Satin L, et al. Genetic and molecular analyses of the C-terminal region of the recE gene from the Rac prophage of Escherichia coli K-12 reveal the recT gene. J Bacteriol. 1993;175:7673-82 pubmed
    The nucleotide sequence of the C-terminal region of the recE gene of the Rac prophage of Escherichia coli K-12 reveals the presence of a partially overlapping reading frame we call recT...
  9. Takahashi N, Yoshikura H, Kobayashi I. An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks. Gene. 2003;303:89-97 pubmed
    ..In a recBC sbcA mutant strain, where recET genes on the Rac prophage are responsible for recombination (RecE pathway), recombination is often conservative, in the sense that it generates two recombinants out of two parental ..
  10. Kowalczykowski S, Dixon D, Eggleston A, Lauder S, Rehrauer W. Biochemistry of homologous recombination in Escherichia coli. Microbiol Rev. 1994;58:401-65 pubmed
    ..At present, this includes the RecA, RecBCD (exonuclease V), RecE (exonuclease VIII), RecF, RecG, RecJ, RecN, RecOR, RecQ, RecT, RuvAB, RuvC, SbcCD, and SSB proteins, as well as DNA ..
  11. Slupska M, Chiang J, Luther W, Stewart J, Amii L, Conrad A, et al. Genes involved in the determination of the rate of inversions at short inverted repeats. Genes Cells. 2000;5:425-37 pubmed
    ..one case, inactivation of the sbcC gene leads to an increase in rearrangements, and in another, insertions near the recE gene lead to an even larger increase...
  12. Shiraishi K, Hanada K, Iwakura Y, Ikeda H. Roles of RecJ, RecO, and RecR in RecET-mediated illegitimate recombination in Escherichia coli. J Bacteriol. 2002;184:4715-21 pubmed
    We analyzed effects of overexpression of RecE and RecT on illegitimate recombination during prophage induction in Escherichia coli and found that frequencies of spontaneous and UV-induced illegitimate recombination are enhanced by ..
  13. Kaiser K, Murray N. On the nature of sbcA mutations in E. coli K 12. Mol Gen Genet. 1980;179:555-63 pubmed
    ..The Rac genome contains recE, the determinant for the ATP-independent exonuclease, ExoVIII. E...
  14. Handa N, Kobayashi I. Type III restriction is alleviated by bacteriophage (RecE) homologous recombination function but enhanced by bacterial (RecBCD) function. J Bacteriol. 2005;187:7362-73 pubmed
    ..restriction system, EcoP1 or EcoP15, is increased by the bacteriophage-mediated homologous recombination functions recE and recT of Rac prophage...
  15. Joseph J, Kolodner R. Exonuclease VIII of Escherichia coli. II. Mechanism of action. J Biol Chem. 1983;258:10418-24 pubmed
    ..The products of degradation of linear duplex DNA were an equivalent amount of 5'-mononucleotides and single-stranded DNA. Possible roles for exonuclease VIII in genetic recombination are discussed. ..
  16. Kuzminov A. Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda. Microbiol Mol Biol Rev. 1999;63:751-813, table of contents pubmed
    ..Putting our knowledge about recombinational repair in the broader context of DNA replication will guide future experimentation. ..
  17. Zhang J, Xing X, Herr A, Bell C. Crystal structure of E. coli RecE protein reveals a toroidal tetramer for processing double-stranded DNA breaks. Structure. 2009;17:690-702 pubmed publisher
    Escherichia coli RecE protein is part of the classical RecET recombination system that has recently been used in powerful new methods for genetic engineering...
  18. Joseph J, Kolodner R. Exonuclease VIII of Escherichia coli. I. Purification and physical properties. J Biol Chem. 1983;258:10411-7 pubmed
    Exonuclease VIII is an enzyme whose synthesis is induced as a result of sbcA mutations...
  19. Ikeda H, Shiraishi K, Ogata Y. Illegitimate recombination mediated by double-strand break and end-joining in Escherichia coli. Adv Biophys. 2004;38:3-20 pubmed
    ..In addition, we have shown that the overexpression of RecE and RecT enhances the frequencies of spontaneous and UV-induced illegitimate recombination and that the RecJ, RecF, ..