Gene Symbol: rcsF
Description: putative outer membrane protein
Alias: ECK0196, JW0192
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Potrykus J, Wegrzyn G. The ypdI gene codes for a putative lipoprotein involved in the synthesis of colanic acid in Escherichia coli. FEMS Microbiol Lett. 2004;235:265-71 pubmed
    ..This phenotype depends on the function of the rcsA gene, but not on that of rcsF. These results suggest that the ypdI gene product might be an additional factor playing a role in colanic acid ..
  2. Laubacher M, Ades S. The Rcs phosphorelay is a cell envelope stress response activated by peptidoglycan stress and contributes to intrinsic antibiotic resistance. J Bacteriol. 2008;190:2065-74 pubmed publisher
    ..Both activation of the phosphorelay and survival required signal transduction via the outer membrane lipoprotein RcsF and the response regulator RcsB...
  3. Sato T, Takano A, Hori N, Izawa T, Eda T, Sato K, et al. Role of the inner-membrane histidine kinase RcsC and outer-membrane lipoprotein RcsF in the activation of the Rcs phosphorelay signal transduction system in Escherichia coli. Microbiology. 2017;163:1071-1080 pubmed publisher
    ..The outer-membrane lipoprotein RcsF is an essential component of the Rcs system...
  4. Konovalova A, Perlman D, Cowles C, Silhavy T. Transmembrane domain of surface-exposed outer membrane lipoprotein RcsF is threaded through the lumen of β-barrel proteins. Proc Natl Acad Sci U S A. 2014;111:E4350-8 pubmed publisher
    b>RcsF (regulator of capsule synthesis) is an outer membrane (OM) lipoprotein that functions to sense defects such as changes in LPS...
  5. Umekawa M, Miyagawa H, Kondo D, Matsuoka S, Matsumoto K, Hara H. Importance of the proline-rich region for the regulatory function of RcsF, an outer membrane lipoprotein component of the Escherichia coli Rcs signal transduction system. Microbiology. 2013;159:1818-27 pubmed publisher
    The outer membrane lipoprotein RcsF is an essential component of the Rcs phosphorelay signal transduction system in Escherichia coli...
  6. Castanie Cornet M, Treffandier H, Francez Charlot A, Gutierrez C, Cam K. The glutamate-dependent acid resistance system in Escherichia coli: essential and dual role of the His-Asp phosphorelay RcsCDB/AF. Microbiology. 2007;153:238-46 pubmed
  7. Castanie Cornet M, Cam K, Jacq A. RcsF is an outer membrane lipoprotein involved in the RcsCDB phosphorelay signaling pathway in Escherichia coli. J Bacteriol. 2006;188:4264-70 pubmed
    ..The other is RcsF, a putative outer membrane lipoprotein mediating the signaling to the sensor RcsC...
  8. Shiba Y, Yokoyama Y, Aono Y, Kiuchi T, Kusaka J, Matsumoto K, et al. Activation of the Rcs signal transduction system is responsible for the thermosensitive growth defect of an Escherichia coli mutant lacking phosphatidylglycerol and cardiolipin. J Bacteriol. 2004;186:6526-35 pubmed
    ..Using transposon mutagenesis, we found that this thermosensitivity was suppressed by disruption of the rcsC, rcsF, and yojN genes, which code for a sensor kinase, accessory positive factor, and phosphotransmitter, respectively, ..
  9. Gupte G, Woodward C, Stout V. Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12. J Bacteriol. 1997;179:4328-35 pubmed
    ..Lastly, several of our rcsB mutants were found to be allele specific (rcsC137 specific) for rcsC, suggesting a physical as well as functional interaction between RcsC and RcsB proteins. ..

More Information


  1. Nagahama H, Oshima T, Mori H, Matsumoto K, Hara H. Hyperexpression of the osmB gene in an acidic phospholipid-deficient Escherichia coli mutant. J Gen Appl Microbiol. 2007;53:143-51 pubmed
  2. Gervais F, Drapeau G. Identification, cloning, and characterization of rcsF, a new regulator gene for exopolysaccharide synthesis that suppresses the division mutation ftsZ84 in Escherichia coli K-12. J Bacteriol. 1992;174:8016-22 pubmed
    A new gene, designated rcsF, was located adjacent to drpA at the 5.2-min position of the genetic map of Escherichia coli...
  3. Leverrier P, Declercq J, Denoncin K, Vertommen D, Hiniker A, Cho S, et al. Crystal structure of the outer membrane protein RcsF, a new substrate for the periplasmic protein-disulfide isomerase DsbC. J Biol Chem. 2011;286:16734-42 pubmed publisher
    ..that includes the histidine kinase (RcsC) and the response regulator (RcsB) and also auxiliary proteins such as RcsF. RcsF is an outer membrane lipoprotein that transmits signals from the cell surface to RcsC...
  4. Shiba Y, Miyagawa H, Nagahama H, Matsumoto K, Kondo D, Matsuoka S, et al. Exploring the relationship between lipoprotein mislocalization and activation of the Rcs signal transduction system in Escherichia coli. Microbiology. 2012;158:1238-48 pubmed publisher
    ..The outer-membrane lipoprotein RcsF is an essential component of Rcs signalling...
  5. Cho S, Szewczyk J, Pesavento C, Zietek M, Banzhaf M, Roszczenko P, et al. Detecting envelope stress by monitoring β-barrel assembly. Cell. 2014;159:1652-64 pubmed publisher
    ..We elucidated how the OM lipoprotein RcsF, the upstream Rcs component, senses envelope stress and activates the signaling cascade...
  6. Konovalova A, Mitchell A, Silhavy T. A lipoprotein/?-barrel complex monitors lipopolysaccharide integrity transducing information across the outer membrane. elife. 2016;5: pubmed publisher
    Lipoprotein RcsF is the OM component of the Rcs envelope stress response...
  7. Callewaert L, Vanoirbeek K, Lurquin I, Michiels C, Aertsen A. The Rcs two-component system regulates expression of lysozyme inhibitors and is induced by exposure to lysozyme. J Bacteriol. 2009;191:1979-81 pubmed publisher
    ..We report activation of this pathway by lysozyme and increased lysozyme sensitivity when Rcs induction is genetically blocked. This lysozyme sensitivity could be alleviated by complementation with Ivy and MliC. ..
  8. Majdalani N, Heck M, Stout V, Gottesman S. Role of RcsF in signaling to the Rcs phosphorelay pathway in Escherichia coli. J Bacteriol. 2005;187:6770-8 pubmed
    ..RcsC, the RcsA coregulator, and the response regulator RcsB, two new components have been characterized, RcsD and RcsF. RcsD, the product of the yojN gene, now renamed rcsD, acts as a phosphorelay between RcsC and RcsB...