Gene Symbol: rcsB
Description: response regulator in two-component regulatory system with RcsC and YojN
Alias: ECK2210, JW2205, viaA
Species: Escherichia coli str. K-12 substr. MG1655

Top Publications

  1. Majdalani N, Heck M, Stout V, Gottesman S. Role of RcsF in signaling to the Rcs phosphorelay pathway in Escherichia coli. J Bacteriol. 2005;187:6770-8 pubmed
    ..In addition to the transmembrane sensor kinase RcsC, the RcsA coregulator, and the response regulator RcsB, two new components have been characterized, RcsD and RcsF...
  2. Boulanger A, Francez Charlot A, Conter A, Castanie Cornet M, Cam K, Gutierrez C. Multistress regulation in Escherichia coli: expression of osmB involves two independent promoters responding either to sigmaS or to the RcsCDB His-Asp phosphorelay. J Bacteriol. 2005;187:3282-6 pubmed
    ..The upstream promoter, osmBp1, is independent of sigma(S) and is activated by RcsB, the response regulator of the His-Asp phosphorelay signal transduction system RcsCDB...
  3. Francez Charlot A, Laugel B, Van Gemert A, Dubarry N, Wiorowski F, Castanie Cornet M, et al. RcsCDB His-Asp phosphorelay system negatively regulates the flhDC operon in Escherichia coli. Mol Microbiol. 2003;49:823-32 pubmed
    ..The regulation is potentiated by the RcsB cofactor RcsA...
  4. Castanie Cornet M, Treffandier H, Francez Charlot A, Gutierrez C, Cam K. The glutamate-dependent acid resistance system in Escherichia coli: essential and dual role of the His-Asp phosphorelay RcsCDB/AF. Microbiology. 2007;153:238-46 pubmed
    The RcsCDB signal transduction system is an atypical His-Asp phosphorelay. Notably, the response regulator RcsB can be activated either by phosphorylation through the RcsCD pathway or by an accessory cofactor RcsA...
  5. Salscheider S, Jahn A, Schnetz K. Transcriptional regulation by BglJ-RcsB, a pleiotropic heteromeric activator in Escherichia coli. Nucleic Acids Res. 2014;42:2999-3008 pubmed publisher
    The bacterial Rcs phosphorelay signals perturbations of the bacterial cell envelope to its response regulator RcsB, which regulates transcription of multiple loci related to motility, biofilm formation and various stress responses...
  6. Majdalani N, Gottesman S. The Rcs phosphorelay: a complex signal transduction system. Annu Rev Microbiol. 2005;59:379-405 pubmed
    RcsC, RcsB, and RcsA were first identified as a sensor kinase, a response regulator, and an auxiliary regulatory protein, respectively, regulating the genes of capsular polysaccharide synthesis...
  7. Sturny R, Cam K, Gutierrez C, Conter A. NhaR and RcsB independently regulate the osmCp1 promoter of Escherichia coli at overlapping regulatory sites. J Bacteriol. 2003;185:4298-304 pubmed
    ..The distal promoter, osmCp1, is activated by NhaR and RcsB. NhaR is a positive regulator of the LysR family and is known to be an activator of the nhaA gene encoding an Na(+)/..
  8. Mouslim C, Latifi T, Groisman E. Signal-dependent requirement for the co-activator protein RcsA in transcription of the RcsB-regulated ugd gene. J Biol Chem. 2003;278:50588-95 pubmed
    The RcsC/YojN/RcsB phosphorelay system controls gene expression in response to a variety of signals, including changes in temperature, osmolarity, and overproduction of membrane proteins...
  9. Castanie Cornet M, Cam K, Bastiat B, Cros A, Bordes P, Gutierrez C. Acid stress response in Escherichia coli: mechanism of regulation of gadA transcription by RcsB and GadE. Nucleic Acids Res. 2010;38:3546-54 pubmed publisher
    ..We have previously shown by genetic approaches that as well as GadE, the response regulator of the Rcs system, RcsB is absolutely required for control of gadA/BC transcription...

More Information


  1. Venkatesh G, Kembou Koungni F, Paukner A, Stratmann T, Blissenbach B, Schnetz K. BglJ-RcsB heterodimers relieve repression of the Escherichia coli bgl operon by H-NS. J Bacteriol. 2010;192:6456-64 pubmed publisher
    b>RcsB is the response regulator of the complex Rcs two-component system, which senses perturbations in the outer membrane and peptidoglycan layer...
  2. Brill J, Quinlan Walshe C, Gottesman S. Fine-structure mapping and identification of two regulators of capsule synthesis in Escherichia coli K-12. J Bacteriol. 1988;170:2599-611 pubmed
    ..b>RcsB, a positive regulator for transcription of the structural genes of colanic acid synthesis (cps), is a protein of ..
  3. Sledjeski D, Gottesman S. A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli. Proc Natl Acad Sci U S A. 1995;92:2003-7 pubmed
    ..coli. Since the stimulation of rcsA transcription by this small RNA does not depend on any sequences from within the rcsA transcript, DsrA acts, either directly or indirectly, on rcsA transcription initiation. ..
  4. Carballès F, Bertrand C, Bouche J, Cam K. Regulation of Escherichia coli cell division genes ftsA and ftsZ by the two-component system rcsC-rcsB. Mol Microbiol. 1999;34:442-50 pubmed
    Genes rcsC and rcsB form a two-component system in which rcsC encodes the sensor element and rcsB the regulator...
  5. Laubacher M, Ades S. The Rcs phosphorelay is a cell envelope stress response activated by peptidoglycan stress and contributes to intrinsic antibiotic resistance. J Bacteriol. 2008;190:2065-74 pubmed publisher
    ..and survival required signal transduction via the outer membrane lipoprotein RcsF and the response regulator RcsB. We propose that the Rcs pathway responds to peptidoglycan damage and contributes to the intrinsic resistance of E...
  6. Wehland M, Bernhard F. The RcsAB box. Characterization of a new operator essential for the regulation of exopolysaccharide biosynthesis in enteric bacteria. J Biol Chem. 2000;275:7013-20 pubmed
    The interaction of the two transcriptional regulators RcsA and RcsB with a specific operator is a common mechanism in the activation of capsule biosynthesis in enteric bacteria...
  7. Francez Charlot A, Castanie Cornet M, Gutierrez C, Cam K. Osmotic regulation of the Escherichia coli bdm (biofilm-dependent modulation) gene by the RcsCDB His-Asp phosphorelay. J Bacteriol. 2005;187:3873-7 pubmed
    ..The regulation is direct and requires an RcsB box next to the bdm -35 element. In addition, bdm is shown to be activated by osmotic shock in an Rcs-dependent way. ..
  8. Stratmann T, Pul U, Wurm R, Wagner R, Schnetz K. RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants. Mol Microbiol. 2012;83:1109-23 pubmed publisher
    ..Here, we show that the heterodimer of the FixJ/NarL-type transcription regulators RcsB and BglJ strongly activates expression of leuO and that RcsB-BglJ regulates additional loci...
  9. Kelley W, Georgopoulos C. Positive control of the two-component RcsC/B signal transduction network by DjlA: a member of the DnaJ family of molecular chaperones in Escherichia coli. Mol Microbiol. 1997;25:913-31 pubmed
    ..Our genetic analysis suggests that DjIA acts in concert with the RcsB/C two-component signal transduction system to augment induction of the cps (capsular polysaccharide) operon and ..
  10. Davalos Garcia M, Conter A, Toesca I, Gutierrez C, Cam K. Regulation of osmC gene expression by the two-component system rcsB-rcsC in Escherichia coli. J Bacteriol. 2001;183:5870-6 pubmed
    ..This work reports the identification of an additional regulatory element, the two-component system rcsB-rcsC, affecting positively the distal promoter osmCp(1)...
  11. Sule P, Wadhawan T, Carr N, Horne S, Wolfe A, Prüss B. A combination of assays reveals biomass differences in biofilms formed by Escherichia coli mutants. Lett Appl Microbiol. 2009;49:299-304 pubmed publisher
    ..The ATP assay, in combination with other quantitative and qualitative assays, will allow us to perform genetic studies on the regulatory network that underlies the early steps in E. coli biofilm formation. ..
  12. Filippova E, Wawrzak Z, Ruan J, Pshenychnyi S, Schultz R, Wolfe A, et al. Crystal structure of nonphosphorylated receiver domain of the stress response regulator RcsB from Escherichia coli. Protein Sci. 2016;25:2216-2224 pubmed publisher
    b>RcsB, the transcription-associated response regulator of the Rcs phosphorelay two-component signal transduction system, activates cell stress responses associated with desiccation, cell wall biosynthesis, cell division, virulence, biofilm ..
  13. Corbett D, Roberts I. Capsular polysaccharides in Escherichia coli. Adv Appl Microbiol. 2008;65:1-26 pubmed publisher
  14. Callewaert L, Vanoirbeek K, Lurquin I, Michiels C, Aertsen A. The Rcs two-component system regulates expression of lysozyme inhibitors and is induced by exposure to lysozyme. J Bacteriol. 2009;191:1979-81 pubmed publisher
    ..We report activation of this pathway by lysozyme and increased lysozyme sensitivity when Rcs induction is genetically blocked. This lysozyme sensitivity could be alleviated by complementation with Ivy and MliC. ..
  15. Nagahama H, Oshima T, Mori H, Matsumoto K, Hara H. Hyperexpression of the osmB gene in an acidic phospholipid-deficient Escherichia coli mutant. J Gen Appl Microbiol. 2007;53:143-51 pubmed
  16. Aiso T, Ohki R. Instability of sensory histidine kinase mRNAs in Escherichia coli. Genes Cells. 2003;8:179-87 pubmed
    ..Monocistronic transcripts of the cognate response regulator genes, arcA, rcsB, narP and narL have a half-life of 1.5-3.4 min. mRNAs of the six histidine kinase genes in E...
  17. Gottesman S, Stout V. Regulation of capsular polysaccharide synthesis in Escherichia coli K12. Mol Microbiol. 1991;5:1599-606 pubmed
    ..Two positive regulators, RcsA and RcsB, are necessary for maximal capsule expression...
  18. Stout V. Regulation of capsule synthesis includes interactions of the RcsC/RcsB regulatory pair. Res Microbiol. 1994;145:389-92 pubmed
  19. Chen M, Takeda S, Yamada H, Ishii Y, Yamashino T, Mizuno T. Characterization of the RcsC-->YojN-->RcsB phosphorelay signaling pathway involved in capsular synthesis in Escherichia coli. Biosci Biotechnol Biochem. 2001;65:2364-7 pubmed
    ..This capsular synthesis is regulated by the RcsC (sensor kinase)-->YojN (phosphotransfer intermediate)-->RcsB (response regulator) phosphorelay signal transduction under certain growth conditions...
  20. Gottesman S, Trisler P, Torres Cabassa A. Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes. J Bacteriol. 1985;162:1111-9 pubmed
    ..rcsA and rcsB are positive regulators of capsule synthesis. rcsA is located at min 43 on the E...
  21. Pannen D, Fabisch M, Gausling L, Schnetz K. Interaction of the RcsB Response Regulator with Auxiliary Transcription Regulators in Escherichia coli. J Biol Chem. 2016;291:2357-70 pubmed publisher
    The Rcs phosphorelay is a two-component signal transduction system that is induced by cell envelope stress. RcsB, the response regulator of this signaling system, is a pleiotropic transcription regulator, which is involved in the control ..
  22. Lee Y, Chang C, Kuo C, Chen M, Yu C, Lin P, et al. Subunit oligomerization and substrate recognition of the Escherichia coli ClpYQ (HslUV) protease implicated by in vivo protein-protein interactions in the yeast two-hybrid system. J Bacteriol. 2003;185:2393-401 pubmed
    ..Thus, we demonstrated in vivo hetero- and homointeractions of ClpQ and ClpY molecules, as well as a direct association between ClpY and substrate SulA, thereby supporting previous in vitro biochemical findings...
  23. Sim S, Yeom J, Shin C, Song W, Shin E, Kim H, et al. Escherichia coli ribonuclease III activity is downregulated by osmotic stress: consequences for the degradation of bdm mRNA in biofilm formation. Mol Microbiol. 2010;75:413-25 pubmed publisher
    ..that downregulation of the ribonucleolytic activity of RNase III is required for the sustained elevation of RcsB-induced bdm mRNA levels during osmotic stress and that cells overexpressing bdm form biofilms more efficiently...
  24. Tschowri N, Busse S, Hengge R. The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light response of Escherichia coli. Genes Dev. 2009;23:522-34 pubmed publisher
    ..These include YmgA and YmgB, which, via the RcsC/RcsD/RcsB two-component phosphorelay system, activate production of the biofilm matrix substance colanic acid as well as acid ..
  25. Schwan W, Shibata S, Aizawa S, Wolfe A. The two-component response regulator RcsB regulates type 1 piliation in Escherichia coli. J Bacteriol. 2007;189:7159-63 pubmed
    ..The orientation depends upon the ratios of the FimB and FimE recombinases. Here, we report that the two-component response regulator RcsB influences the piliation state by controlling fimB and fimE transcription.
  26. Shiba Y, Yokoyama Y, Aono Y, Kiuchi T, Kusaka J, Matsumoto K, et al. Activation of the Rcs signal transduction system is responsible for the thermosensitive growth defect of an Escherichia coli mutant lacking phosphatidylglycerol and cardiolipin. J Bacteriol. 2004;186:6526-35 pubmed
    ..Disruption of the rcsB gene coding for the response regulator of the system also suppressed the thermosensitivity, whereas disruption of ..
  27. Stout V, Gottesman S. RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli. J Bacteriol. 1990;172:659-69 pubmed
    Colanic acid capsule synthesis in Escherichia coli K-12 is regulated by RcsB and RcsC...
  28. Castanie Cornet M, Cam K, Jacq A. RcsF is an outer membrane lipoprotein involved in the RcsCDB phosphorelay signaling pathway in Escherichia coli. J Bacteriol. 2006;188:4264-70 pubmed
    ..One is RcsA, which can stimulate the activity of the response regulator RcsB independently of the phosphorelay to regulate a subset of RcsB targets...
  29. Majdalani N, Hernandez D, Gottesman S. Regulation and mode of action of the second small RNA activator of RpoS translation, RprA. Mol Microbiol. 2002;46:813-26 pubmed
    ..DsrA synthesis is increased at low temperature. We find that RprA synthesis is regulated by the RcsC/RcsB phosphorelay system, previously found to regulate capsule synthesis and promoters of ftsZ and osmC...
  30. Jayaratne P, Keenleyside W, MacLachlan P, Dodgson C, Whitfield C. Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides. J Bacteriol. 1993;175:5384-94 pubmed
    In Escherichia coli K-12, RcsC and RcsB are thought to act as the sensor and effector components, respectively, of a two-component regulatory system which regulates expression of the slime polysaccharide colanic acid (V. Stout and S...
  31. Parkinson J. Signal transduction schemes of bacteria. Cell. 1993;73:857-71 pubmed
  32. Stock J, Stock A, Mottonen J. Signal transduction in bacteria. Nature. 1990;344:395-400 pubmed publisher
    ..This phosphotransfer network couples environmental signals to an array of response elements that control cell motility and regulate gene expression...
  33. Ogasawara H, Hasegawa A, Kanda E, Miki T, Yamamoto K, Ishihama A. Genomic SELEX search for target promoters under the control of the PhoQP-RstBA signal relay cascade. J Bacteriol. 2007;189:4791-9 pubmed
    ..With these data taken together, we conclude that the expression of both asr and csgD is under the direct control of the PhoQP-RstBA signal relay cascade. ..
  34. Houng H, Noon K, Ou J, Baron L. Expression of Vi antigen in Escherichia coli K-12: characterization of ViaB from Citrobacter freundii and identity of ViaA with RcsB. J Bacteriol. 1992;174:5910-5 pubmed
    ..Two widely separated chromosomal regions, ViaA and ViaB, are needed for Vi synthesis...
  35. Schmöe K, Rogov V, Rogova N, Lohr F, Güntert P, Bernhard F, et al. Structural insights into Rcs phosphotransfer: the newly identified RcsD-ABL domain enhances interaction with the response regulator RcsB. Structure. 2011;19:577-87 pubmed publisher
    ..component serves as a crucial factor in modulating the phosphorylation state of the transcription factor RcsB. We have identified a new domain, RcsD-ABL, located N terminally to RcsD-HPt that interacts with RcsB as well...
  36. Torres Cabassa A, Gottesman S. Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis. J Bacteriol. 1987;169:981-9 pubmed
    ..Our results are consistent with a model in which the presence of a lon mutation increases the synthesis of capsular polysaccharide via stabilization of RcsA. ..
  37. Dierksen K, Trempy J. Identification of a second RcsA protein, a positive regulator of colanic acid capsular polysaccharide genes, in Escherichia coli. J Bacteriol. 1996;178:5053-6 pubmed
    ..Ferguson plot analysis suggests that the two RcsA proteins differ by size rather than by charge. Both RcsA proteins are expressed from a single rcsA gene. Detection of both RcsA proteins in delta lon cells is RcsB dependent.
  38. Peterson C, Carabetta V, Chowdhury T, Silhavy T. LrhA regulates rpoS translation in response to the Rcs phosphorelay system in Escherichia coli. J Bacteriol. 2006;188:3175-81 pubmed
    ..In addition, mutations in the gene encoding the DNA motor protein FtsK induce lrhA synthesis, which may explain why such strains fail to accumulate RpoS in stationary phase. ..
  39. Breddermann H, Schnetz K. Correlation of Antagonistic Regulation of leuO Transcription with the Cellular Levels of BglJ-RcsB and LeuO in Escherichia coli. Front Cell Infect Microbiol. 2016;6:106 pubmed
    ..in Escherichia coli leuO is antagonistically regulated by the heterodimeric transcription regulator BglJ-RcsB and by LeuO. BglJ-RcsB activates leuO, while LeuO inhibits activation by BglJ-RcsB...
  40. Fredericks C, Shibata S, Aizawa S, Reimann S, Wolfe A. Acetyl phosphate-sensitive regulation of flagellar biogenesis and capsular biosynthesis depends on the Rcs phosphorelay. Mol Microbiol. 2006;61:734-47 pubmed
    ..Acetyl approximately P-dependent capsular biosynthesis required both RcsB and RcsA, while the lack of RcsC restored capsular biosynthesis to acetyl approximately P-deficient cells...
  41. Sledjeski D, Gottesman S. Osmotic shock induction of capsule synthesis in Escherichia coli K-12. J Bacteriol. 1996;178:1204-6 pubmed
    ..Two of these, RcsB and RcsC, share homology with two-component regulatory elements that respond to environmental stimuli...
  42. Johnson M, Burton N, Guti rrez B, Painter K, Lund P. RcsB is required for inducible acid resistance in Escherichia coli and acts at gadE-dependent and -independent promoters. J Bacteriol. 2011;193:3653-6 pubmed publisher
    b>RcsB interacts with GadE to mediate acid resistance in stationary-phase Escherichia coli K-12...
  43. Ferrières L, Clarke D. The RcsC sensor kinase is required for normal biofilm formation in Escherichia coli K-12 and controls the expression of a regulon in response to growth on a solid surface. Mol Microbiol. 2003;50:1665-82 pubmed
    ..Therefore, we suggest that the RcsC sensor kinase may play an important role in the remodelling of the bacterial surface during growth on a solid surface and biofilm formation. ..
  44. Huang Y, Ferrières L, Clarke D. The role of the Rcs phosphorelay in Enterobacteriaceae. Res Microbiol. 2006;157:206-12 pubmed
    The Rcs phosphorelay is composed of the sensor kinase, RcsC, the HPt-domain protein RcsD and the response regulator, RcsB. In this review we discuss the role of the Rcs phosphorelay in the Enterobacteriaceae, highlighting the observation ..
  45. Arslan Z, Stratmann T, Wurm R, Wagner R, Schnetz K, Pul m. RcsB-BglJ-mediated activation of Cascade operon does not induce the maturation of CRISPR RNAs in E. coli K12. RNA Biol. 2013;10:708-15 pubmed publisher
    ..This activation is LeuO-dependent as heterodimers of BglJ and RcsB activate leuO transcription...
  46. Shiba Y, Matsumoto K, Hara H. DjlA negatively regulates the Rcs signal transduction system in Escherichia coli. Genes Genet Syst. 2006;81:51-6 pubmed
    ..These results demonstrate that DjlA functions as a negative regulator of the Rcs system unless abnormally overproduced. ..
  47. Ferrières L, Aslam S, Cooper R, Clarke D. The yjbEFGH locus in Escherichia coli K-12 is an operon encoding proteins involved in exopolysaccharide production. Microbiology. 2007;153:1070-80 pubmed
    ..Finally, evidence is provided that the overexpression of yjbEFGH affects colony morphology and leads to the production of an extracellular polysaccharide that binds Congo red and toluidine blue-O. ..
  48. Gupte G, Woodward C, Stout V. Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12. J Bacteriol. 1997;179:4328-35 pubmed
    Regulation of colanic acid polysaccharide capsule synthesis in Escherichia coli requires the proteins RcsC and RcsB, in addition to several other proteins...
  49. Parkinson J, Kofoid E. Communication modules in bacterial signaling proteins. Annu Rev Genet. 1992;26:71-112 pubmed
  50. Hengge R. The two-component network and the general stress sigma factor RpoS (sigma S) in Escherichia coli. Adv Exp Med Biol. 2008;631:40-53 pubmed publisher
    ..These systems are involved in the control of motility, biofilm formation and/or virulence, suggesting that further studying a potential role of RpoS in these physiological functions may be rewarding. ..
  51. Stout V, Torres Cabassa A, Maurizi M, Gutnick D, Gottesman S. RcsA, an unstable positive regulator of capsular polysaccharide synthesis. J Bacteriol. 1991;173:1738-47 pubmed
    ..contains a possible helix-turn-helix DNA-binding motif that resembles sequences found at the carboxy terminus of RcsB, another positive regulator of capsule synthesis, and in several other transcriptional regulators including members ..
  52. Conter A, Sturny R, Gutierrez C, Cam K. The RcsCB His-Asp phosphorelay system is essential to overcome chlorpromazine-induced stress in Escherichia coli. J Bacteriol. 2002;184:2850-3 pubmed
    ..This induction is abolished in rcsB or rcsC mutant strains. In addition, treatment with CPZ inhibits growth...
  53. Hirakawa H, Nishino K, Yamada J, Hirata T, Yamaguchi A. Beta-lactam resistance modulated by the overexpression of response regulators of two-component signal transduction systems in Escherichia coli. J Antimicrob Chemother. 2003;52:576-82 pubmed
    ..Thirteen response regulators out of 32 genes, namely baeR, cheY, cpxR, creB, evgA, fimZ, narL, ompR, rcsB, rstA, yedW, yehT and dcuR, conferred increased beta-lactam resistance...
  54. Glover W, Yang Y, Zhang Y. Insights into the molecular basis of L-form formation and survival in Escherichia coli. PLoS ONE. 2009;4:e7316 pubmed publisher
    ..Four (Group 1) mutants, rcsB, a positive response regulator of colanic acid capsule synthesis, ruvA, a recombinational junction binding protein, ..
  55. Hu L, Chi B, Kuhn M, Filippova E, Walker Peddakotla A, Bäsell K, et al. Acetylation of the response regulator RcsB controls transcription from a small RNA promoter. J Bacteriol. 2013;195:4174-86 pubmed publisher
    ..To help answer these questions, we studied the Escherichia coli response regulator and transcription factor RcsB, which is reported to be acetylated in vitro...
  56. Vianney A, Jubelin G, Renault S, Dorel C, Lejeune P, Lazzaroni J. Escherichia coli tol and rcs genes participate in the complex network affecting curli synthesis. Microbiology. 2005;151:2487-97 pubmed
    ..In the tol mutants, activation of csgDEFG occurred via OmpR and was dominant upon repression by RcsB and RcsA, while these two regulatory proteins repressed csgBA through a dominant effect on the activator protein ..
  57. Stout V. Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12. J Bacteriol. 1996;178:4273-80 pubmed
    ..of proteins involved in regulating the expression of these genes includes the two positive regulators RcsA and RcsB. This work describes the site of action of these two activator proteins and the promoter of the cps genes...
  58. Kuo M, Chen K, Wu W. Regulation of RcsA by the ClpYQ (HslUV) protease in Escherichia coli. Microbiology. 2004;150:437-46 pubmed
    ..By contrast, overproduction of cellular ClpYQ resulted in decreased beta-galactosidase levels of RcsA(Red)-LacZ. Taken together, the data indicate that ClpYQ acts as a secondary protease in degrading the Lon substrate RcsA. ..
  59. Rogov V, Bernhard F, Lohr F, Dötsch V. Solution structure of the Escherichia coli YojN histidine-phosphotransferase domain and its interaction with cognate phosphoryl receiver domains. J Mol Biol. 2004;343:1035-48 pubmed
    ..The activity of the central regulator RcsB is modulated by phosphorylation through the sensor kinases YojN and RcsC, with the YojN histidine ..
  60. Morgan J, Vendura K, Stevens S, Riordan J. RcsB determines the locus of enterocyte effacement (LEE) expression and adherence phenotype of Escherichia coli O157 : H7 spinach outbreak strain TW14359 and coordinates bicarbonate-dependent LEE activation with repression of motility. Microbiology. 2013;159:2342-53 pubmed publisher
    ..the unique virulence expression and adherence phenotype of this strain, and further expands the role for regulator RcsB in control of the E. coli locus of enterocyte effacement (LEE) pathogenicity island...
  61. Krin E, Danchin A, Soutourina O. RcsB plays a central role in H-NS-dependent regulation of motility and acid stress resistance in Escherichia coli. Res Microbiol. 2010;161:363-71 pubmed publisher
    ..In the present study, suppressor mutations were identified in the rcsB gene...
  62. Takeda S, Fujisawa Y, Matsubara M, Aiba H, Mizuno T. A novel feature of the multistep phosphorelay in Escherichia coli: a revised model of the RcsC --> YojN --> RcsB signalling pathway implicated in capsular synthesis and swarming behaviour. Mol Microbiol. 2001;40:440-50 pubmed
    In this study, we re-investigated the previously characterized RcsC (sensor His-kinase) --> RcsB (response regulator) phosphorelay system that is involved in the regulation of capsular polysaccharide synthesis in Escherichia coli...